The document analyzes the nutritional composition of Moringa oleifera leaves of the South African ecotype. It finds the dried leaves contain 30.3% crude protein with 19 amino acids. Notable mineral contents include 3.65% calcium, 1.5% potassium, and 0.3% phosphorus. Fatty acid analysis identified 17 fatty acids, with alpha-linolenic acid at 44.57%. Vitamin E was the highest vitamin at 77 mg/100g. The fiber contents were 11.4% NDF and 8.49% ADF. Overall, the amino acids, fatty acids, minerals, and vitamins reflected a desirable nutritional balance for the Moringa leaves.
Identification and evaluation of antifungal compounds from botanicals for the...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus, Colletotrichum falcatum. In this study, eighteen different botanicals were screened for identifying effective antifungal compound against C. falcatum. Among the plants screened, 15 per cent aqueous leaf extract of Psoralea corylifolia alone inhibited 100 per cent growth of both mycelium as well as spore germination under in vitro conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes viz., Pseudomonas fluorescens, Bacillus megaterium and Gluconacetobacter diazotrophicus which are normally used in sugarcane. The effective plant extracts exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC-MS analysis to identify the bioactive antifungal compound. It revealed the presence of 7H-furo [3,2-G] (1) benzopyran-7-one as main bioactive compound which is thought to be the intermediate of antifungal compound, 8 – methoxypsoralen formed during biosynthesis.
Article Citation:
Rajkumar D and Murugesan R.
Identification and Evaluation of Antifungal Compounds from Botanicals for the Control of Sugarcane Red Rot Pathogen, Colletotrichum falcatum.
Journal of Research in Agriculture (2013) 2(1): 164-172.
Full Text:
http://www.jagri.info/documents/AG0044.pdf
Screening and evaluation of medicinal properties of grape fruit varieties in ...EditorIJAERD
Theni districts could be considered one of the highest grapes producing area also export best quality of
Paneer dhrakshai Indian table grapes in Tamil Nadu. The research work focused to identify the nutritive compounds in
the grapes (Vitis vinifera) species which are a wonderful fruit having pulp content, vibrant color and exert an immense
health benefits. Grape fruits were found to be rich source of Anthocyanin content and vitamin C also macromolecules
contents such as carbohydrate contents of 29mg, Protein 18.7mg and fat content 3.7mg /100 g of f. wt. Micronutrients
were estimated by AAS which showed that calcium content was 3.1mg/g.f.wt, iron content 2.7 mg/g.f.wt, potassium 2.6
mg/g.f.wt found to be more than other minerals in fresh extracts of grape fruits. The comparative results of revealed
considerable amount of phytoconstituents in grape fruits in which anthocyanins alone has been taken to evaluate
therapeutic effects on breast cancer cell MCF-7 cell viability test was carried out to determine cell proliferation. The
results revealed presence of anthocyanins in high quantity in the process of purification through various biochemical
methods that could ultimately be used for preventing cancerous cell proliferation which reduces the development of
cancer diseases. On comparison Grapes (Vitis vinifera) may serve as functional food with vital pharmaceutical and
biological value.
Study of Bioactive Components Present in Oil Extracted from Fenugreek Seed: R...AI Publications
A fenugreek seed (Trigonella foenum-graecum) is a native of India, and also found in Mediterranean region, southern Europe, Africa and western Asia. These seeds have their importance due to the medicinal properties as well as nutraceutical value present in their chemical composition. This fenugreek seeds were used for treating diabetes, menstrual cramps, cancer, heart related disease, skin and hair fall treatments, high per tension, weight loss, to increase milk supply in women who were breastfeeding. This seeds is found to be abundant in N-Dimethylaminomethyl, 1-ptoprnol, 2, 2-dimethyl-aceto, Aziridine,1,2,3-trimethyl-trans, 2-pentanone,4-hydroxy-4 meth, 2-trimethylsily-1,3-dithiane, 3-0-methyl-d-glucose, d-mannopyranoiside,uronate,Diazidine1,3,3-trimethyl,Methyl 4,8dimethylnonanoate There are numerous methods available on extraction of these compounds right from conventional (Maceration, Percolation, Reflux extraction, Soxhlet extraction, microwave assist extraction, pressurised liquid extraction, super critical fluid extraction, Pulsed electric field extraction, Enzyme assisted extraction, Ultrasound assisted extraction) apart from this some water based extraction technique may also use to extract bioactive compounds from fenugreek seeds. In present article a focus has been given to highlight importance of fenugreek seeds extract in terms of extraction of bioactive compounds and their probable application in the area of food and pharmaceuticals.
Comparative study between the effects of mango and orange peels preparations ...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online.
Fruit and Vegetable Waste Hydrolysates as Growth Medium for Higher Biomass an...Premier Publishers
Fruit and vegetable wastes include peels, pulp and seeds that constitute about 40% of the total mass and constitute huge environmental problems. Cultivation of microalgae that utilizes fruit and vegetable wastes as feedstock to produce value added products such as biomass and lipids is a unique approach. Different concentrations of fruit waste hydrolysate (FWH) and vegetable waste hydrolysate (VWH) were used for heterotropic cultivation of Chlorella vulgaris thereby optimizing the suitable hydrolysate concentration for higher biomass and lipid production. FWH in the ratio of 8:2 has produced maximum specific growth rate of 1.92 µ d-1. Higher biomass was recorded in growth medium supplemented with FWH (0.16 mg L-1) than VWH medium. Highest chlorophyll content of 7.2 mg L-1 was observed in 8:2 ratio of FWH whereas it was 4.3 mg L-1 in VWH at the same concentration. Carotenoid content was highest in VWH than FWH media with a maximum content of 0.52 and 0.42 mg L-1 respectively. Fruit waste hydrolysates significantly increased the total lipid content than the vegetable waste hydrolysate medium. Highest lipid content of 6.63 mg L-1 was recorded in 8:2 ratio of FWH. This work demonstrates the feasibility of fruit waste hydrolysate as a nutrient source for algal cultivation and a cost reduction of growth medium in algal biomass and lipid production.
1 ijfaf oct-2017-4-the characteristics of rumenAI Publications
The ruminant animal feed ingredients consisting of forage with the combination of grass and legume which were almost limited in availability, the cocoa leaves as agricultural wastes or wastes containing low nutrient plantation low used as alternative feed ingredients. To overcome these problems required a simple technology that is fermentation using microorganisms indigenus so that the effluent quality cocoa can be improved. Indigenus microorganisms were microbes that exist in individuals without disturbing the activities. The purpose of this research was to improve the quality of rumen fluid characteristics and digestibility of cocoa leaves fermented by microorganisms indigenus. The method used in this study was completely randomized design factorial with three replications, treatment factors consist of A as the level of microbes (A1 = 5%, A2 = 10%, A3 = 15%) and factor B as the duration of fermentation (B1 = 2 day, B2 = 4 days, B3 = 6 days) and the variables; the nutrient content and digestibility of cocoa leaves before and after being fermented and the characteristics of rumen fluid in vitro methods. The results showed that the increasing levels of use of microbes and fermentation time can enhance the quality of cocoa leaves that reduce the content of dry material from 31.389 to 25.488% and increase the protein content of the coarse 6.153 to 7.483%. Dry Material digestibility increased from 27.379 to 32.942% and 27.432% Protein 21,435- rough. Rumen fluid characteristics were rumen fluid pH ranges from 7.427 to 7.603, NH3 production ranges from 16.820 - 19,975mg / 100ml, and Volatyl Fatty Acid production from 83.667 to 101.000 mM. The conclusion that increasing the digestibility quality cocoa fruit leather could be made by fermentation by microorganisms indigenus.
Identification and evaluation of antifungal compounds from botanicals for the...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus, Colletotrichum falcatum. In this study, eighteen different botanicals were screened for identifying effective antifungal compound against C. falcatum. Among the plants screened, 15 per cent aqueous leaf extract of Psoralea corylifolia alone inhibited 100 per cent growth of both mycelium as well as spore germination under in vitro conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes viz., Pseudomonas fluorescens, Bacillus megaterium and Gluconacetobacter diazotrophicus which are normally used in sugarcane. The effective plant extracts exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC-MS analysis to identify the bioactive antifungal compound. It revealed the presence of 7H-furo [3,2-G] (1) benzopyran-7-one as main bioactive compound which is thought to be the intermediate of antifungal compound, 8 – methoxypsoralen formed during biosynthesis.
Article Citation:
Rajkumar D and Murugesan R.
Identification and Evaluation of Antifungal Compounds from Botanicals for the Control of Sugarcane Red Rot Pathogen, Colletotrichum falcatum.
Journal of Research in Agriculture (2013) 2(1): 164-172.
Full Text:
http://www.jagri.info/documents/AG0044.pdf
Screening and evaluation of medicinal properties of grape fruit varieties in ...EditorIJAERD
Theni districts could be considered one of the highest grapes producing area also export best quality of
Paneer dhrakshai Indian table grapes in Tamil Nadu. The research work focused to identify the nutritive compounds in
the grapes (Vitis vinifera) species which are a wonderful fruit having pulp content, vibrant color and exert an immense
health benefits. Grape fruits were found to be rich source of Anthocyanin content and vitamin C also macromolecules
contents such as carbohydrate contents of 29mg, Protein 18.7mg and fat content 3.7mg /100 g of f. wt. Micronutrients
were estimated by AAS which showed that calcium content was 3.1mg/g.f.wt, iron content 2.7 mg/g.f.wt, potassium 2.6
mg/g.f.wt found to be more than other minerals in fresh extracts of grape fruits. The comparative results of revealed
considerable amount of phytoconstituents in grape fruits in which anthocyanins alone has been taken to evaluate
therapeutic effects on breast cancer cell MCF-7 cell viability test was carried out to determine cell proliferation. The
results revealed presence of anthocyanins in high quantity in the process of purification through various biochemical
methods that could ultimately be used for preventing cancerous cell proliferation which reduces the development of
cancer diseases. On comparison Grapes (Vitis vinifera) may serve as functional food with vital pharmaceutical and
biological value.
Study of Bioactive Components Present in Oil Extracted from Fenugreek Seed: R...AI Publications
A fenugreek seed (Trigonella foenum-graecum) is a native of India, and also found in Mediterranean region, southern Europe, Africa and western Asia. These seeds have their importance due to the medicinal properties as well as nutraceutical value present in their chemical composition. This fenugreek seeds were used for treating diabetes, menstrual cramps, cancer, heart related disease, skin and hair fall treatments, high per tension, weight loss, to increase milk supply in women who were breastfeeding. This seeds is found to be abundant in N-Dimethylaminomethyl, 1-ptoprnol, 2, 2-dimethyl-aceto, Aziridine,1,2,3-trimethyl-trans, 2-pentanone,4-hydroxy-4 meth, 2-trimethylsily-1,3-dithiane, 3-0-methyl-d-glucose, d-mannopyranoiside,uronate,Diazidine1,3,3-trimethyl,Methyl 4,8dimethylnonanoate There are numerous methods available on extraction of these compounds right from conventional (Maceration, Percolation, Reflux extraction, Soxhlet extraction, microwave assist extraction, pressurised liquid extraction, super critical fluid extraction, Pulsed electric field extraction, Enzyme assisted extraction, Ultrasound assisted extraction) apart from this some water based extraction technique may also use to extract bioactive compounds from fenugreek seeds. In present article a focus has been given to highlight importance of fenugreek seeds extract in terms of extraction of bioactive compounds and their probable application in the area of food and pharmaceuticals.
Comparative study between the effects of mango and orange peels preparations ...iosrjce
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT) multidisciplinary peer-reviewed Journal with reputable academics and experts as board member. IOSR-JESTFT is designed for the prompt publication of peer-reviewed articles in all areas of subject. The journal articles will be accessed freely online.
Fruit and Vegetable Waste Hydrolysates as Growth Medium for Higher Biomass an...Premier Publishers
Fruit and vegetable wastes include peels, pulp and seeds that constitute about 40% of the total mass and constitute huge environmental problems. Cultivation of microalgae that utilizes fruit and vegetable wastes as feedstock to produce value added products such as biomass and lipids is a unique approach. Different concentrations of fruit waste hydrolysate (FWH) and vegetable waste hydrolysate (VWH) were used for heterotropic cultivation of Chlorella vulgaris thereby optimizing the suitable hydrolysate concentration for higher biomass and lipid production. FWH in the ratio of 8:2 has produced maximum specific growth rate of 1.92 µ d-1. Higher biomass was recorded in growth medium supplemented with FWH (0.16 mg L-1) than VWH medium. Highest chlorophyll content of 7.2 mg L-1 was observed in 8:2 ratio of FWH whereas it was 4.3 mg L-1 in VWH at the same concentration. Carotenoid content was highest in VWH than FWH media with a maximum content of 0.52 and 0.42 mg L-1 respectively. Fruit waste hydrolysates significantly increased the total lipid content than the vegetable waste hydrolysate medium. Highest lipid content of 6.63 mg L-1 was recorded in 8:2 ratio of FWH. This work demonstrates the feasibility of fruit waste hydrolysate as a nutrient source for algal cultivation and a cost reduction of growth medium in algal biomass and lipid production.
1 ijfaf oct-2017-4-the characteristics of rumenAI Publications
The ruminant animal feed ingredients consisting of forage with the combination of grass and legume which were almost limited in availability, the cocoa leaves as agricultural wastes or wastes containing low nutrient plantation low used as alternative feed ingredients. To overcome these problems required a simple technology that is fermentation using microorganisms indigenus so that the effluent quality cocoa can be improved. Indigenus microorganisms were microbes that exist in individuals without disturbing the activities. The purpose of this research was to improve the quality of rumen fluid characteristics and digestibility of cocoa leaves fermented by microorganisms indigenus. The method used in this study was completely randomized design factorial with three replications, treatment factors consist of A as the level of microbes (A1 = 5%, A2 = 10%, A3 = 15%) and factor B as the duration of fermentation (B1 = 2 day, B2 = 4 days, B3 = 6 days) and the variables; the nutrient content and digestibility of cocoa leaves before and after being fermented and the characteristics of rumen fluid in vitro methods. The results showed that the increasing levels of use of microbes and fermentation time can enhance the quality of cocoa leaves that reduce the content of dry material from 31.389 to 25.488% and increase the protein content of the coarse 6.153 to 7.483%. Dry Material digestibility increased from 27.379 to 32.942% and 27.432% Protein 21,435- rough. Rumen fluid characteristics were rumen fluid pH ranges from 7.427 to 7.603, NH3 production ranges from 16.820 - 19,975mg / 100ml, and Volatyl Fatty Acid production from 83.667 to 101.000 mM. The conclusion that increasing the digestibility quality cocoa fruit leather could be made by fermentation by microorganisms indigenus.
Terry Exports LLP (TEL) is an Indian based premium moringa powder exports-oriented firm, which promotes, market and distributes moringa oleifera products of the highest quality to pharmaceutical/nutraceuticals, cosmetic, personal care, wellness, food, beverage and spa industries in the USA, Europe.
Study on Characterization of Various Biofilms Prepared by Starch Isolated fro...ijtsrd
In the present study, the rhizome of Maranta arundinacea L., Arrowroot, was selected for a rich source of starch for the preparation of biofilm. Firstly, some physicochemical properties of the selected sample were determined by AOAC method. Furthermore, the elemental analysis of the selected sample was carried out by Energy Dispersive X ray Fluorescence EDXRF spectroscopy. Moreover, antimicrobial activities of various solvent extracts were examined by Agar well diffusion method on six tested organisms. And then, the qualitative determination of starch tests such as Iodine test and Tannic acid test were done. In addition, starch from Arrowroot powder was isolated and confirmed by FT IR spectrum. Finally, starch biofilms were prepared by using isolated starch and various ratios of plasticizers PVA, PEG, and Sorbitol. The characterizations of seven kinds of prepared biofilms were measured. Aye Mon Thida Nyo | Arnt Win | Baby San Chit Su | Mar Pi Myint | Phyu Phyu Khaing "Study on Characterization of Various Biofilms Prepared by Starch Isolated from Maranta Arundinacea L." Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26588.pdfPaper URL: https://www.ijtsrd.com/chemistry/other/26588/study-on-characterization-of-various-biofilms-prepared-by-starch-isolated-from-maranta-arundinacea-l/aye-mon-thida-nyo
The anti-nutritional composition of white, brown, spotted and black coat coloured African yam
bean varieties that are cultivated in the Afikpo town of Ebonyi State in Nigeria was determined in this study. The
oxalate, trypsin, inhibitor phytate, and tannin contents were determined. The results of the oxalate composition
showed that the white variety was lowest with the value of 0.949 mg/100, while the brown variety was highest
with the value of 5.973 mg/100. The trypsin, phytate and tannin contents ranged from 0.689 mg/l to 0.981 mg/l,
0.002 mg/100 to 0.003 mg/100 and 0.005 % to 0.008 %. There were no significant differences in the
compositions of inhibitor phytate and tannin in the four varieties of the African yam bean. The findings suggest
that the intake of the brown African yam bean should be minimized to avoid hindrance to calcium and
magnesium metabolism because of the high oxalate content.
Poster presentation on the research article carried out during Internship period LEE presented in National Horticulture society Seminar Held in Kirtipur.
Phytochemical Screening, Proximate and Elemental Analyses of Senna siamea (La...Premier Publishers
Senna siamea has been used by various ethnicities in Nigeria for the treatment of various ailments including syphilis, herpes, swine fever, typhoid fever, jaundice, abdominal pain, menstrual pain, among others. In this study, the leaves of Senna siamea were collected and made to undergo proximate, phytochemical and elemental analyses. Result for the proximate analysis revealed 7.43 % moisture content, 20.62 % ash content, 3.00 % lipid content, 21.88 % protein content, 13.00 % crude fibre content and 34.07 % carbohydrate content. Phytochemical screening results indicated high presence of terpenoids, tannins and volatile oils; moderate presence of alkaloids and cardiac glycosides; low presence of saponins, steroids, flavonoids and general glycosides. Quantitative elemental analysis revealed the presence of iron, magnesium and calcium in appreciable amounts of 11.03 %, 10.83 % and 3.08 % respectively. The secondary metabolites contained in S. siamea leaves are hugely responsible for its use in the afore-mentioned treatments and the appreciable amounts of vital minerals suggest they could be processed and inculcated in animal feeds.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
PHYTO-NUTRITIONAL PROFILES OF BROOM WEED (SIDA ACUTA) LEAF EXTRACTSubmissionResearchpa
Medicinal plants are abundant in phytochemicals that has significant therapeutic effects. They are relatively cheap, effective and safe in prolong use. This experiment was carried out to investigate the phyto-nutritional profile of broom weed (Sida acuta) leaf extract. Proximate analysis Sida acuta leaf indicated the presence of dry matter (91.88 %), crude protein (18.01 %), ash (9.73 %), crude fibre (6.24 %), ether extract (1.77 %) and energy (2760 Kcal/kg). Phytochemical screening of the extract revealed the presence of condensed tannins, hydrolysable tannins, flavonoids, saponins, phenols, oxalate, phytate, alkaloids, terpenoids and glycosides at 0.82 %, 2.02 %, 4.25 %, 0.20 %, 0.17 %, 0.05 %, 0.23 %, 0.22 %, 0.95 % and 0.02 % respectively and the vitamin constituents are thiamine (0.33 mg/100g), ascorbic acid (30.17 mg/100g), riboflavin (0.05 mg/100g), β-carotene (0.79 mg/100g) and niacin (0.41 mg/100g). Mineral analysis showed that it contained calcium (127.6 mg/100g), phosphorus (78.6 mg/100g), potassium (31.6 mg/100g), magnesium (102.1 mg/100g), iron (2.14 mg/100g), manganese (0.60 mg/100g), copper (0.04 mg/100g) and zinc (1.75 mg/100g). Result on amino acid composition showed that they contained lysine (2.11 mg/100g), arginine (1.77 mg/100g), aspartic acid (2.13 mg/100g), threonine (1.85 mg/100g), histidine (4.71 mg/100g), serine (2.03 mg/100g), glycine (1.05 mg/100g), alanine (3.31 mg/100g), cystine (5.06 mg/100g), valine (0.88 mg/100g), leucine (2.04 mg/100g), phenyalanine (4.72 mg/100g), tyrosine (3.51 mg/100g), isoleucine (2.84 mg/100g), methionine (0.85 mg/100g) and proline (1.05 mg/100g). It was concluded that Sida acuta leaf extract is rich in various nutrients and phytochemicals conferring it the ability to perform multiple biological activities and as a natural alternative to antibiotics. by Shittu, M. D and Alagbe, J.O 2020. PHYTO-NUTRITIONAL PROFILES OF BROOM WEED (SIDA ACUTA) LEAF EXTRACT. International Journal on Integrated Education. 3, 11 (Nov. 2020), 119-124. DOI:https://doi.org/10.31149/ijie.v3i11.845. https://journals.researchparks.org/index.php/IJIE/article/view/845/806 https://journals.researchparks.org/index.php/IJIE/article/view/845
Ecological environment effects on germination and seedling morphology in Park...AI Publications
Néré (Parkia biglobosa) is a wild species preferred and overexploited for its multiple uses by rural populations in Sub-Saharan Africa. The study of its germination and seedlings could constitute a prerequisite for its domestication, necessary for its conservation. This study aimed to assess the germination and morphology of seedlings taking into account distinct habitats from its natural environment.A total of 2160 seeds from different mother plants and 540 seedlings from germination were selected and evaluated. The trials were conducted on three sites (two nurseries in Côte d'Ivoire vs one greenhouse in France) with different microclimates. The results showed that the larger the mother trees are, the larger the seeds they produce, which in turn generate more vigorous seedlings. This study showed that the species grows better in a milder environment that is different from its region of origin (fertile soil with a stable or humid tropical climate: Montpellier greenhouse and Daloa nursery). Overall, parent trees did not statistically influence each germination and seedling development parameter for the three sites combined (P > 0.05). However, analysis of variance showed that germination and seedling development parameters differed between experimental sites (P < 0.05). These results are useful and could be used as decision support tools to guide conservation (domestication) and agroforestry programmes based on Parkia biglobosa. This study could be extended to other endangered species in order to preserve biodiversity.
The study was carried out to investigate the effect of the aqueous extracts of
Myristicafragrans(Nutmeg), Murrayakoenigi(curry leaf) and Aframomummelegueta(Guinea pepper) on Some
Biochemical and haematologicalParameters. Sixteen (16) wister strain rats weighing between 130 – 180g
were divided into four (4) groups of four (4) rats each and for 21 days fed the following diets: Group A –
normal diet + myristicafragrans (Nutmeg) aqueous extract, Group B – normal diet + murrayakoenigi (curry
leaf) aqueous extract, Group C – normal diet + aframomummelegueta (Guinea pepper) aqueous extract, Group
D – normal diet (control). After a period of 21 days the rats were sacrificed and the serum was taken for the
following estimations: total protein, albumin, total bilirubin, direct bilirubin, aspartate transaminase, alanine
transaminase, alkaline phosphatase, total cholesterol, triglyceride, HDL cholesterol, LDL cholesterol and
glucose. The whole blood was taken for packed cell volume and white blood cell count. The results indicated
that oral administration of myristicafragrans, murrayakoenigi and aframomummelegueta to rat’s exhibit
remarkable hypolipidaemic activity and lowering glucose concentration. The oral administration of these
three spices exhibit protein increasing activities compared with the control rats. The packed cell volume and
white cell values of all the rats decreased after feeding with experimental diet (aqueous extract) compare
with the control rats. It is clear from this study thatMyristicafragrans(Nutmeg), Murrayakoenigi(curry leaf)
andAframomummelegueta (Guinea pepper) contain significant amounts of phytochemicals and exhibit
hypolipidaemic activity when consumed.
RESIDUAL ESTIMATION OF ISOPROTURON, ATRAZINE AND GRAIN PROTECTANTS IN STORED ...ijabjournal
This study was carried out to see the occurrence and level of pesticide residues in stored wheat grain samples. Wheat grains were collected from different godowns located in Faisalabad district. Weedicide residues in wheat grains were analyzed by using thin plate liquid chromatography and validated high
performance liquid chromatography. On the basis of extent of inhibition of the Hill reaction, it was found that the level of atrazine was (0-0.028 ngkg-1) and the level of isopproturon was (0-0.024 ngkg-1) in test samples. The calculated values were below the Maximum Residue Limit (MEL) i.e. 100 ngkg-1 for atrazine and 2200 ngkg-1 for isoproturon. It was found that presence of herbicide residues in stored wheat was negligible. Further, on wheat grain samples placed in jars and treated with deltamethrin and cypermethrin @0.4 and 0.8% concentration. The persistency data was calculated after 1 hour, 7, 15 and 30 days of treatment. The grain protectants were continuously degraded with the passage of time and after the 30 days
the amount of residues of the grain protectants was found to be very small. It was concluded that the use of the grain protectants in the powder form in combination with other botanicals on stored wheat samples is feasible to reduce the insect pest in stored grains.
A Study on the Removal of Pesticide Residues on Potatoes Using Moringa oleife...AI Publications
Moringa oleifera (MO) is a plant whose seeds possess strong coagulation properties capable of removing turbidity and heavy metals from water. In this study, the effectiveness of MO seeds as a potential pesticide removal apparatus for agricultural produce was investigated. The parameters of interest for each pesticide washing solution were pesticide-concentration reduction, cost, and taste alteration to the potatoes. Separate doses of 0.25 mL profenofos 500 EC were individually applied to distinct 250 g pesticide-free potato piles. A submersion of each tainted potato pile was carried out in one of the chosen pesticide baths (mineral water, 2% salt solution, 10% salt solution, vegetable bath wash, and 10 g MO seed) for 10 min, followed by a 10 second mineral water bath rinse. Remaining profenofos residue concentrations on potatoes were quantified using the quick, easy, cheap, rugged, and simple QuEChERs method coincided with High Performance Liquid Chromatography (HPLC). The experiments showed that the MO seed was the most effective at removing profenofos at 52.9% followed by the vegetable bath at 47.3%. Neither method caused an alteration in food taste, but the MO seed method is significantly cheaper at 2,000/L IDR ($0.14 USD) compared to the vegetable bath at IDR 10,668/L ($0.77 USD) at the time of the experiment, respectively.
Use of date syrup as alternative carbon source for microbial cultivationPremier Publishers
In the present work, date syrup and date fruit soaked water as alternative carbon source for biomass production of Bacillus megaterium as model organism was optimized. Maximum biomass production was obtained on 2.8, 4.1 g/l for molasses and date fruits soaked respectively. This source was substantially greater than could be attained on media that used various other carbon sources. The optimal medium for producing the biomass was a mineral medium formulated with 8% of date syrup as the carbon source and 0.5 g/L (NH4)2SO4as the nitrogen source. At optimal fermentation time of 48 hrs, at 30oC.Water soaked and date syrup can be used to inexpensively produce biomassin batch fermentations using B. megaterium as phosphorus solubilizing soil bacteria. Farther more studies should be focused on agriculture cheapest sources as nature alternatives for carbon ear nitrogen sources.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
Free radical scavenging activity, phytochemistry and antimicrobial properties...Premier Publishers
This study is aimed at investigating the phytochemistry, free radical scavenging activity and antimicrobial properties of Tetrapleura tetraptera seeds. The results of the analysis of Tetrapleura tetraptera seeds showed the presence of phenols (0.34 %), flavonoids (0.91 %), alkaloids (0.52 %), tannins (0.23 %) and saponins (0.51 %). Vitamins include ascorbic acid (5.23 %), riboflavin (0.11 %), niacin (1.25 %),-carotenoid (3.40 %) and thiamine (0.06 %). Proximate compositions were moisture (14.73 %), ash (7.30 %), crude protein (17.36 %), crude fibre (6.68 %) and lipids (35.19 %). Mineral elements were calcium (1.41 %), magnesium (0.48 %), potassium (0.56 %), sodium (0.21 %), phosphorus (0.32 %), iron (1.20 %), copper (0.13 %) and zinc (0.77 %). The seed extract showed potent free radical scavenging activity (6.72 – 22.8 %) using ascorbic acid as a standard (12.10 - 42.00 %). The seed extract exhibited significant antimicrobial activity (7.00 – 20.00 mm) and the zones of inhibition were compared with that of ciprofloxacin as standard (15.00 – 28.00 mm). From these investigations, seed extract of Tetrapleura tetraptera has proven to be considered an excellent source of nutriceuticals, a potential antioxidant and antimicrobial agents owing to its high level of phytochemical and vitamin constituents.
Terry Exports LLP (TEL) is an Indian based premium moringa powder exports-oriented firm, which promotes, market and distributes moringa oleifera products of the highest quality to pharmaceutical/nutraceuticals, cosmetic, personal care, wellness, food, beverage and spa industries in the USA, Europe.
Study on Characterization of Various Biofilms Prepared by Starch Isolated fro...ijtsrd
In the present study, the rhizome of Maranta arundinacea L., Arrowroot, was selected for a rich source of starch for the preparation of biofilm. Firstly, some physicochemical properties of the selected sample were determined by AOAC method. Furthermore, the elemental analysis of the selected sample was carried out by Energy Dispersive X ray Fluorescence EDXRF spectroscopy. Moreover, antimicrobial activities of various solvent extracts were examined by Agar well diffusion method on six tested organisms. And then, the qualitative determination of starch tests such as Iodine test and Tannic acid test were done. In addition, starch from Arrowroot powder was isolated and confirmed by FT IR spectrum. Finally, starch biofilms were prepared by using isolated starch and various ratios of plasticizers PVA, PEG, and Sorbitol. The characterizations of seven kinds of prepared biofilms were measured. Aye Mon Thida Nyo | Arnt Win | Baby San Chit Su | Mar Pi Myint | Phyu Phyu Khaing "Study on Characterization of Various Biofilms Prepared by Starch Isolated from Maranta Arundinacea L." Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26588.pdfPaper URL: https://www.ijtsrd.com/chemistry/other/26588/study-on-characterization-of-various-biofilms-prepared-by-starch-isolated-from-maranta-arundinacea-l/aye-mon-thida-nyo
The anti-nutritional composition of white, brown, spotted and black coat coloured African yam
bean varieties that are cultivated in the Afikpo town of Ebonyi State in Nigeria was determined in this study. The
oxalate, trypsin, inhibitor phytate, and tannin contents were determined. The results of the oxalate composition
showed that the white variety was lowest with the value of 0.949 mg/100, while the brown variety was highest
with the value of 5.973 mg/100. The trypsin, phytate and tannin contents ranged from 0.689 mg/l to 0.981 mg/l,
0.002 mg/100 to 0.003 mg/100 and 0.005 % to 0.008 %. There were no significant differences in the
compositions of inhibitor phytate and tannin in the four varieties of the African yam bean. The findings suggest
that the intake of the brown African yam bean should be minimized to avoid hindrance to calcium and
magnesium metabolism because of the high oxalate content.
Poster presentation on the research article carried out during Internship period LEE presented in National Horticulture society Seminar Held in Kirtipur.
Phytochemical Screening, Proximate and Elemental Analyses of Senna siamea (La...Premier Publishers
Senna siamea has been used by various ethnicities in Nigeria for the treatment of various ailments including syphilis, herpes, swine fever, typhoid fever, jaundice, abdominal pain, menstrual pain, among others. In this study, the leaves of Senna siamea were collected and made to undergo proximate, phytochemical and elemental analyses. Result for the proximate analysis revealed 7.43 % moisture content, 20.62 % ash content, 3.00 % lipid content, 21.88 % protein content, 13.00 % crude fibre content and 34.07 % carbohydrate content. Phytochemical screening results indicated high presence of terpenoids, tannins and volatile oils; moderate presence of alkaloids and cardiac glycosides; low presence of saponins, steroids, flavonoids and general glycosides. Quantitative elemental analysis revealed the presence of iron, magnesium and calcium in appreciable amounts of 11.03 %, 10.83 % and 3.08 % respectively. The secondary metabolites contained in S. siamea leaves are hugely responsible for its use in the afore-mentioned treatments and the appreciable amounts of vital minerals suggest they could be processed and inculcated in animal feeds.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
PHYTO-NUTRITIONAL PROFILES OF BROOM WEED (SIDA ACUTA) LEAF EXTRACTSubmissionResearchpa
Medicinal plants are abundant in phytochemicals that has significant therapeutic effects. They are relatively cheap, effective and safe in prolong use. This experiment was carried out to investigate the phyto-nutritional profile of broom weed (Sida acuta) leaf extract. Proximate analysis Sida acuta leaf indicated the presence of dry matter (91.88 %), crude protein (18.01 %), ash (9.73 %), crude fibre (6.24 %), ether extract (1.77 %) and energy (2760 Kcal/kg). Phytochemical screening of the extract revealed the presence of condensed tannins, hydrolysable tannins, flavonoids, saponins, phenols, oxalate, phytate, alkaloids, terpenoids and glycosides at 0.82 %, 2.02 %, 4.25 %, 0.20 %, 0.17 %, 0.05 %, 0.23 %, 0.22 %, 0.95 % and 0.02 % respectively and the vitamin constituents are thiamine (0.33 mg/100g), ascorbic acid (30.17 mg/100g), riboflavin (0.05 mg/100g), β-carotene (0.79 mg/100g) and niacin (0.41 mg/100g). Mineral analysis showed that it contained calcium (127.6 mg/100g), phosphorus (78.6 mg/100g), potassium (31.6 mg/100g), magnesium (102.1 mg/100g), iron (2.14 mg/100g), manganese (0.60 mg/100g), copper (0.04 mg/100g) and zinc (1.75 mg/100g). Result on amino acid composition showed that they contained lysine (2.11 mg/100g), arginine (1.77 mg/100g), aspartic acid (2.13 mg/100g), threonine (1.85 mg/100g), histidine (4.71 mg/100g), serine (2.03 mg/100g), glycine (1.05 mg/100g), alanine (3.31 mg/100g), cystine (5.06 mg/100g), valine (0.88 mg/100g), leucine (2.04 mg/100g), phenyalanine (4.72 mg/100g), tyrosine (3.51 mg/100g), isoleucine (2.84 mg/100g), methionine (0.85 mg/100g) and proline (1.05 mg/100g). It was concluded that Sida acuta leaf extract is rich in various nutrients and phytochemicals conferring it the ability to perform multiple biological activities and as a natural alternative to antibiotics. by Shittu, M. D and Alagbe, J.O 2020. PHYTO-NUTRITIONAL PROFILES OF BROOM WEED (SIDA ACUTA) LEAF EXTRACT. International Journal on Integrated Education. 3, 11 (Nov. 2020), 119-124. DOI:https://doi.org/10.31149/ijie.v3i11.845. https://journals.researchparks.org/index.php/IJIE/article/view/845/806 https://journals.researchparks.org/index.php/IJIE/article/view/845
Ecological environment effects on germination and seedling morphology in Park...AI Publications
Néré (Parkia biglobosa) is a wild species preferred and overexploited for its multiple uses by rural populations in Sub-Saharan Africa. The study of its germination and seedlings could constitute a prerequisite for its domestication, necessary for its conservation. This study aimed to assess the germination and morphology of seedlings taking into account distinct habitats from its natural environment.A total of 2160 seeds from different mother plants and 540 seedlings from germination were selected and evaluated. The trials were conducted on three sites (two nurseries in Côte d'Ivoire vs one greenhouse in France) with different microclimates. The results showed that the larger the mother trees are, the larger the seeds they produce, which in turn generate more vigorous seedlings. This study showed that the species grows better in a milder environment that is different from its region of origin (fertile soil with a stable or humid tropical climate: Montpellier greenhouse and Daloa nursery). Overall, parent trees did not statistically influence each germination and seedling development parameter for the three sites combined (P > 0.05). However, analysis of variance showed that germination and seedling development parameters differed between experimental sites (P < 0.05). These results are useful and could be used as decision support tools to guide conservation (domestication) and agroforestry programmes based on Parkia biglobosa. This study could be extended to other endangered species in order to preserve biodiversity.
The study was carried out to investigate the effect of the aqueous extracts of
Myristicafragrans(Nutmeg), Murrayakoenigi(curry leaf) and Aframomummelegueta(Guinea pepper) on Some
Biochemical and haematologicalParameters. Sixteen (16) wister strain rats weighing between 130 – 180g
were divided into four (4) groups of four (4) rats each and for 21 days fed the following diets: Group A –
normal diet + myristicafragrans (Nutmeg) aqueous extract, Group B – normal diet + murrayakoenigi (curry
leaf) aqueous extract, Group C – normal diet + aframomummelegueta (Guinea pepper) aqueous extract, Group
D – normal diet (control). After a period of 21 days the rats were sacrificed and the serum was taken for the
following estimations: total protein, albumin, total bilirubin, direct bilirubin, aspartate transaminase, alanine
transaminase, alkaline phosphatase, total cholesterol, triglyceride, HDL cholesterol, LDL cholesterol and
glucose. The whole blood was taken for packed cell volume and white blood cell count. The results indicated
that oral administration of myristicafragrans, murrayakoenigi and aframomummelegueta to rat’s exhibit
remarkable hypolipidaemic activity and lowering glucose concentration. The oral administration of these
three spices exhibit protein increasing activities compared with the control rats. The packed cell volume and
white cell values of all the rats decreased after feeding with experimental diet (aqueous extract) compare
with the control rats. It is clear from this study thatMyristicafragrans(Nutmeg), Murrayakoenigi(curry leaf)
andAframomummelegueta (Guinea pepper) contain significant amounts of phytochemicals and exhibit
hypolipidaemic activity when consumed.
RESIDUAL ESTIMATION OF ISOPROTURON, ATRAZINE AND GRAIN PROTECTANTS IN STORED ...ijabjournal
This study was carried out to see the occurrence and level of pesticide residues in stored wheat grain samples. Wheat grains were collected from different godowns located in Faisalabad district. Weedicide residues in wheat grains were analyzed by using thin plate liquid chromatography and validated high
performance liquid chromatography. On the basis of extent of inhibition of the Hill reaction, it was found that the level of atrazine was (0-0.028 ngkg-1) and the level of isopproturon was (0-0.024 ngkg-1) in test samples. The calculated values were below the Maximum Residue Limit (MEL) i.e. 100 ngkg-1 for atrazine and 2200 ngkg-1 for isoproturon. It was found that presence of herbicide residues in stored wheat was negligible. Further, on wheat grain samples placed in jars and treated with deltamethrin and cypermethrin @0.4 and 0.8% concentration. The persistency data was calculated after 1 hour, 7, 15 and 30 days of treatment. The grain protectants were continuously degraded with the passage of time and after the 30 days
the amount of residues of the grain protectants was found to be very small. It was concluded that the use of the grain protectants in the powder form in combination with other botanicals on stored wheat samples is feasible to reduce the insect pest in stored grains.
A Study on the Removal of Pesticide Residues on Potatoes Using Moringa oleife...AI Publications
Moringa oleifera (MO) is a plant whose seeds possess strong coagulation properties capable of removing turbidity and heavy metals from water. In this study, the effectiveness of MO seeds as a potential pesticide removal apparatus for agricultural produce was investigated. The parameters of interest for each pesticide washing solution were pesticide-concentration reduction, cost, and taste alteration to the potatoes. Separate doses of 0.25 mL profenofos 500 EC were individually applied to distinct 250 g pesticide-free potato piles. A submersion of each tainted potato pile was carried out in one of the chosen pesticide baths (mineral water, 2% salt solution, 10% salt solution, vegetable bath wash, and 10 g MO seed) for 10 min, followed by a 10 second mineral water bath rinse. Remaining profenofos residue concentrations on potatoes were quantified using the quick, easy, cheap, rugged, and simple QuEChERs method coincided with High Performance Liquid Chromatography (HPLC). The experiments showed that the MO seed was the most effective at removing profenofos at 52.9% followed by the vegetable bath at 47.3%. Neither method caused an alteration in food taste, but the MO seed method is significantly cheaper at 2,000/L IDR ($0.14 USD) compared to the vegetable bath at IDR 10,668/L ($0.77 USD) at the time of the experiment, respectively.
Use of date syrup as alternative carbon source for microbial cultivationPremier Publishers
In the present work, date syrup and date fruit soaked water as alternative carbon source for biomass production of Bacillus megaterium as model organism was optimized. Maximum biomass production was obtained on 2.8, 4.1 g/l for molasses and date fruits soaked respectively. This source was substantially greater than could be attained on media that used various other carbon sources. The optimal medium for producing the biomass was a mineral medium formulated with 8% of date syrup as the carbon source and 0.5 g/L (NH4)2SO4as the nitrogen source. At optimal fermentation time of 48 hrs, at 30oC.Water soaked and date syrup can be used to inexpensively produce biomassin batch fermentations using B. megaterium as phosphorus solubilizing soil bacteria. Farther more studies should be focused on agriculture cheapest sources as nature alternatives for carbon ear nitrogen sources.
Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
Free radical scavenging activity, phytochemistry and antimicrobial properties...Premier Publishers
This study is aimed at investigating the phytochemistry, free radical scavenging activity and antimicrobial properties of Tetrapleura tetraptera seeds. The results of the analysis of Tetrapleura tetraptera seeds showed the presence of phenols (0.34 %), flavonoids (0.91 %), alkaloids (0.52 %), tannins (0.23 %) and saponins (0.51 %). Vitamins include ascorbic acid (5.23 %), riboflavin (0.11 %), niacin (1.25 %),-carotenoid (3.40 %) and thiamine (0.06 %). Proximate compositions were moisture (14.73 %), ash (7.30 %), crude protein (17.36 %), crude fibre (6.68 %) and lipids (35.19 %). Mineral elements were calcium (1.41 %), magnesium (0.48 %), potassium (0.56 %), sodium (0.21 %), phosphorus (0.32 %), iron (1.20 %), copper (0.13 %) and zinc (0.77 %). The seed extract showed potent free radical scavenging activity (6.72 – 22.8 %) using ascorbic acid as a standard (12.10 - 42.00 %). The seed extract exhibited significant antimicrobial activity (7.00 – 20.00 mm) and the zones of inhibition were compared with that of ciprofloxacin as standard (15.00 – 28.00 mm). From these investigations, seed extract of Tetrapleura tetraptera has proven to be considered an excellent source of nutriceuticals, a potential antioxidant and antimicrobial agents owing to its high level of phytochemical and vitamin constituents.
Studies on the Crude Extract of Moringa Oleifera Leaf for Preliminary Identif...iosrjce
IOSR Journal of Applied Chemistry (IOSR-JAC) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of applied chemistry and its applications. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Chemical Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Proximate Analysis, Mineral Contents and Functional Properties of Moringa Ole...IOSR Journals
Abstract: Freshly harvested Moringa oleifera was authenticated and processed for its vegetable leaf protein concentrates and analysed for proximate composition, functional properties and mineral composition. The proximate analysis was evaluated using standard analytical procedures. The proximate analysis reveal the moisture content to be 9.00mg/100g, ash content 6.00mg/100g, crude fat 2.43mg/100g, crude fibre 5.43mg/100g, crude protein 39.13mg/100g and carbohydrate content is 38.21 mg/100g. The mineral content of the leaves indicated that Ca, Mg, Zn, Mn, and Na are the most abundant. The water absorption capacity, fat absorption capacity, emulsion capacity, foaming stability revealed good functional properties.
Phytochemical, Proximate and Nutrient Analysis of Cassia Tora Seedsinventionjournals
Recently Proximate analysis and phytochemical analysis and nutrient analysis of seeds of Cassia tora L. from the campus of Government Vidharbha Institute of Science and Humanity, Amravati had been investigated. The seeds sample contained tannin, saponin, protein, steriods, terpenoids, carbohydrate, alkaloids, flavonoids and glycosides. Proximate analysis of moisture, ash, fat amd mineral analysis of calcium, magnesium, iron, nitrogen and solubility were check. The values of it is moisture (56%), cold water (52%), hot water (54%), 1%NaOH (43%), 1%HCl (61%), benzene +alcohol (35%), ash content (17%). These results indicate that the seeds of these Cassia tora L. contains mineral and nutrients elements that will be useful in nutrition. Also the existence of some phytochemicals like tannin, saponin and steroids illustrated medicinal action of the plant in its therapeutic uses. The result of their phytochemcial screening could justify the observed activities and validate their use in herbal medicine.
Phytochemical Analysis and Mineral Content of Ethanol Extract of Citrullus la...Premier Publishers
This study examined the phytochemical and elemental content of water melon (Citrullus lanatus) bark. The sample was spread in the laboratory to dry at room temperature after which it was grounded to get a coarse powder form using sterile mortar and pestle. The extracts (ethanol and aqueous) were subjected to phytochemical and elemental analysis using standard procedures. Qualitative phytochemical screening of the ethanol extract revealed the presence of tannins, alkaloid, saponins, oxalates, flavonoids, steroid, phytates and glycoside while the quantitative phytochemical screening indicated the contents of tannins to be (1.360g/100g), alkaloid (3.965 g/100g), saponins (1.380 g/100g), oxalates (3.675 g/100g), flavonoids (1.145g/100g), steroid (1.080g/100g), phytates (3.160g/100g) and glycoside (4.490 g/100g). The elemental analysis showed that the extracts contains Ca (0.025±0.002mg/100g), K (0.065±0.001mg/100g), Fe (0.189±0.007mg/100g), Zn (0.263±0.005mg/100g), Mg (1.771±0.005 mg/100g), Mn (0.670 ±0.003mg/100g), Na (0.033±0.001mg/100g), and Cu (0.245±0.003mg/100g) however Cr was not detected. The results of this study showed that the ethanol extract of C. lanatus contain bioactive compounds which might be responsible for pharmacological actions of the plant and the plant can also serve as a source of mineral elements in diet.
Phytochemical screening and proximate composition of the sea grasses Enhalus ...Innspub Net
Knowledge of the chemical composition of seagrass is important both for the assessment of the nutritional value of marine invertebrate or vertebrate herbivores and for the evaluation of potential sources of protein, carbohydrates, and lipids for commercial use or for possible human consumption. While phytochemical screening provides basic information about the medicinal importance of the plant extract and are lead compound for drug discoveries. Proximate composition and phytochemical analyses were determined on the seagrass Enhalus acoroides and Thalassia hemprichii using standard methods on a dry basis. The result of the proximate composition showed that Enhalus acoroides contained 9.35% crude protein, 0.07% crude lipid, 54.94% carbohydrate contents and 35.64% ash. While Thalassia hemprichii contained 14.98% crude protein, 0.17% crude lipid, 52.87% carbohydrate contents and 31.98% ash. The phytochemicals detected in the methanolic extracts of Enhalus acoroides were alkaloids, flavonoids, saponins, phenols, tannins, and steroids. While for Thalassia hemprichii were alkaloids, flavonoids, saponins, phenols, tannins, triterpenoids and steroids. The results indicate that seagrass Enhalus acoroides and Thalassia hemprichii are good potential sources of highly nutritious feed stuff and the different active secondary metabolites along with significant bioactive potential might be helpful for the future pharmaceutical applications.
Nutrient and Bioactive Potentials of some Agricultural Food WastesPremier Publishers
The current research investigated the proximate, mineral content and phytochemical constituents of five (5) food wastes, namely: Telfairia occidentalis (fluted pumpkin) seed peels and pod; Artocarpus camansi (breadnut) seed peels and creamy pulp and Mucuna urens (horse eye bean) seed peels. The results of the proximate analysis of the food wastes revealed that the samples have varying percentage range of 51.70-70.25 for carbohydrate, 7.35-11.90 for protein, 1.90-6.20 for fat, 2.80-11.60 for fiber, 3.40-15.25 for ash and 4.40-18.15 for moisture. The percentage carbohydrate content showed that pumpkin seed peel had the lowest yield while pumpkin pod peel had the highest. The result of the mineral analysis revealed that the five food wastes analysed contain Zn, K, P, Ca, Mg, Fe and Na, however, the levels of Fe, Ca, P and Mg were significantly higher (p<0.05) relative to the other minerals. The phytochemical estimation of the samples showed that the food wastes contain several bioactive compounds in different concentrations. Some of the phytochemicals found in the food wastes are spartein, phytate, anthocyanin, tannin, lunamarin, ribalinidine, catechin, rutin, kaempferol, oxalate, epicatechin, phenol and sapogenin. This study revealed that these food wastes possess some nutritive and medicinal potential.
In Vitro Assessment of Antioxidant Activity, Total Phenolic and Flavonoid Con...AI Publications
Sweet marjoram (Origanum majorana L.) is cultivated as a condiment for its aromatic leaves for culinary purposes and utilized as a medicinal plant for many diseases. The aim of this study was to evaluate in vitro antioxidant activity of marjoram extract by the 2, 2–diphenyl–1–picrylhydrazyl–hydrate (DPPH) free radical scavenging method while total phenolic and flavonoid contents were quantified by spectrophotometry using Folin−Ciocalteu and aluminum chloride colorimetric methods, respectively. The extraction yield of sweet marjoram obtained by maceration in absolute ethanol at a ratio of 1/5 (w/v) for 24h at room temperature was 8.41 ± 0.76 % (w/w). The obtained results showed that the investigated extract contained a higher amount of phenolics: 164.96 ± 4.61 mg GAE/g of dry plant, lower flavonoid contents: 44.61 ± 2.08 mg QE/g of dry plant, and exhibited a strong antioxidant activity (IC50 value: 40.09 µg/ml) almost like those of the used standard products, namely ascorbic acid and butylated hydroxytoluene (BHT). Based on the obtained results, marjoram (Origanum majorana L.) features a potential application as natural antioxidants that could be exploited by the pharmaceutical and food industries.
Twenty two genotypes of quality protein maize (QPM) and two local checks were assessed for their lysine and tryptophan levels, as well as grain yield characteristics at the Lower Niger River Basin Development Authority station, Oke-Oyi, Ilorin, Nigeria for three years (2009-2011). The results showed that the QPM genotypes and the standard checks varied from one another, with respect to crude protein, zein dry matter, zein crude, lysine and tryptophan. The best QPM hybrids for grain yield (Dada-ba, ART98-SW5-OB, ART98-SW4-OB and TZPB-OB had percentage lysine and tryptophan advantage of 34% compared with the local checks. These hybrids also out-yielded other genotypes with yield advantage of 10, 24 and 26% over the best inbred, open pollinated variety and the standard check respectively. However, grain yield showed positive association with all the characteristics except crude protein content. Kernel number per cob had maximum correlation with grain yield followed by kernel rows per cob, cob diameter and cob weight. The direct effect for crude protein was positive but the correlation was negative. Conclusively, the QPM hybrids that combined high yield with the essential amino acids could be tested in different savanna agro-ecologies to identify those that could be released to farmers, while the superior inbreds could be introgressed for further breeding programs.
Effects of Vegetative Zones on the Nutritional Composition of Vernonia Amygda...AI Publications
The study was carried out to determine the effects of vegetative zones (location) on the nutritional compositions of Vernonia amygdalina leaves in Ekiti State, Nigeria. Across locations (in each vegetative zones), A total land area of 9 m x 18 m was acquired and soil samples were randomly collected to determine the physical and chemical composition of the soils. The growth of Vernonia amygdalina (stem) with a spacing of 1 m x 1 m between rows was determined. Harvested fresh leaves of the plant were analyzed for nutritional composition using standard methods. Nutritional and mineral compositions were observed at different proportions in the leaves of the investigated plant. Higher proximate composition in the leaves of Vernonia amygdalina was also recorded in terms of Moisture (Efon; 11.34%) and protein (Ikere; 22.46 %,) in the rainforest. However, higher Fat (Otun; 4.58 %), Crude fiber (Omuo; 16.08 %), Ash (Omuo; 14.69%) and Carbohydrate (Otun; 38.68 %) was found in Vernonia amygdalina grown in the Derived Savannah area. Similarly, the Mineral element compositions in the leaves of Vernonia amygdalina were found to be higher in plant from the rainforest area than the derived savannah area of Ekiti State except sodium and iron, which were higher in Vernonia amygdalina grown in the Derived Savannah area. However, the significant differences recorded between the nutritional components on the studied medicinal plant reveals the potentiality of the influence of soil factor and different climatic conditions of the study areas.
Hepatoprotective and Antioxidant Effects of the Flavonoid-rich Fraction of th...IOSRJPBS
The leaves of Jatropha tanjorensis are edible and used in herbal medicine in the treatment of diseases associated with oxidative stress. The present study demonstrates the antioxidative effect of the flavonoid-rich fraction of the methanol extract of Jatropha tanjorensis leaves (FRJT) against CCl4-induced hepatotoxicity in rats. Hepatoprotective and antioxidant properties of FRJT were determined by serum biochemical enzymes; alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), antioxidant enzymes (SOD, CAT and GPx), heamatological pararmeters (PCV, Hb and WBC) and histology study. The results obtained showed a significant reduction (p < 0.05) in the activities of liver marker enzymes across the pre-treated groups compared with the untreated rats. Assay of antioxidant enzymes showed that the extract significantly (p < 0.05) enhanced SOD and GPx activities whereas CAT activity was non-significantly (p ˃ 0.05) increased when compared with the untreated animals. PCV, Hb and WBC levels were significantly (p < 0.05) lower in the untreated group. However, supplementation with FRJT and Silymarin ameliorated the induced depletion of blood in the pre-treated animals. Histological examination of the liver tissue showed marked reduction in fatty degeneration across the pre-treated groups when compared with the untreated group. The results in this study indicate that FRJT exhibited varying levels of protection against CCl4-induced oxidative stress in rat models. These results also indicate that the flavonoid-rich fraction contains antioxidants, which mop up free radicals in the system and support its use in the treatment of diseases resulting from oxidative damage.
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Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
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Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
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Moringa is a plantfood of high nutritional value, ecologically and economically beneficial and readily available in the countries hardest hit by the food crisis. http://miracletrees.org/ http://moringatrees.org/
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2. 12926 Afr. J. Biotechnol.
Recently, a high degree of renewed interest was placed
on the nutritional properties of Moringa in most countries
where it was not native (Reyes et al., 2006; Oduro et al.,
2008). This could be due to the claims that it increases
animal productivity as it has nutritional, therapeutic and
prophylactic properties (Fahey, 2005). Studies from other
countries indicate that the leaves have immense
nutritional value such as vitamins, minerals and amino
acids (Anwar et al., 2007). As such, the leaves have been
used to combat malnutrition, especially among infants
and nursing mothers. In addition, nutrition plays a crucial
role in both humans and livestock as short-term
alternative to chemoprophylaxis. In animals, nutrition
plays a major role in animal’s ability to overcome the
detrimental effects of parasitism and diseases (Anwar et
al., 2007). A well-nourished animal resists diseases even
when exposed to infection than the one, which is already
weakened through malnutrition. When an animal is
exposed to pathogens, the animal’s immune system
mounts a response to fight off infection. This includes
raising antibodies to fight the infection, as well as using
white blood cells to attack pathogens (FAO, 2002). To
gain immunity, the animal needs energy, proteins for
manufacture of antibodies and cells, minerals (zinc,
copper and iron) and vitamins (A and E) in
communicating messages in parts of the animal’s body to
fight infections (Conroy, 2005).
There are considerable variations among the nutritional
values of Moringa, which depend on factors like genetic
background, environment and cultivation methods
(Brisibe et al., 2009). As such, it necessitates
determination of the nutritive value of Moringa of South
African ecotype, which could assists in the formulation of
diets according to nutrients requirements. The nutritional
composition of Moringa of the South African ecotype has
to our knowledge not previously been evaluated; this is
the first report that includes the profiling of chemical
composition, fatty acids, amino acids and vitamins.
Amino acids, fatty acids, minerals and vitamins are
essential in animal feed. These nutrients are used for
osmotic adjustment; activate enzymes, hormones and
other organic molecules that enhance growth, function
and maintenance of life process (Anjorin et al., 2010).
Nutritional composition of the plant plays a significant role
in nutritional, medicinal and therapeutic values (Al-
Kharusi et al., 2009). In addition, the chemical
composition of the fibre fractions affects the digestibility
of the feed, which directly or indirectly affect the feed’s
utilization by animals. It was reported that nutritional
content in the leaves of Moringa varies with location
(Anjorin et al., 2010). This has prompted the study of
nutritional composition of Moringa of South African
ecotype. Therefore, the objective of the study was to
determine the nutritional value of Moringa leaves of the
South African ecotype.
MATERIALS AND METHODS
Plant collection and preparation
The plant leaves were collected at Sedikong sa Lerato in Tooseng
village Ga-Mphahlele (24°26´57.10˝S, 29°33´47.02˝E), Limpopo
Province of South Africa. The mean annual rainfall of the area is
approximately 300 mm and the mean annual temperature is 15°C.
The plant was authenticated at the University of Fort Hare,
Department of Botany and a voucher specimen (BM 01/2009) was
prepared and deposited in the Giffen Herbarium of the University of
Fort Hare. The leaves were harvested green, air-dried under shade
and milled into powder through 1 mm sieve using Restch Cross
Beater Mill SK 100, Monitoring and Control laboratories (Pty) Ltd,
Parkhurst, South Africa. They were stored in well-dried black plastic
containers inside the storeroom at room temperature of 25°C.
Nutritional composition determination
Dried powdered Moringa leaves were assessed for dry matter (DM),
crude protein (CP), crude fat, calcium (Ca), magnesium (Mg),
potassium (K), phosphorus (P) zinc (Zn), copper (Cu), iron (Fe),
manganese (Mn), selenium (Se) and sodium (Na) using the
Association of Official Agricultural Chemists (AOAC, 2005)
procedures. Neutral detergent fibre (NDF), acid detergent fibre
(ADF), acid detergent lignin (ADL), acid detergent cellulose (ADC)
and hemi-cellulose were determined following the techniques
established by Van Soest et al. (1991).
Condensed tannins and total phenolics determination
Condensed tannins (CT) assays were performed calorimetrically
with butanol-HCl method (Bate-Smith, 1981) using purified CT from
Desmodium intortum as a reference standard. This method is
based on oxidative cleavage of the interflavan bonds in the
presence of mineral acids in alcoholic solutions at about 95°C to
yield pink coloured anthocyanidins, which are measured at 550 nm.
Total phenolics were assayed calorimetrically according to Price
and Butler (1977). In this method, 6 ml of aqueous solution of
phenolics, 50 ml of distilled water were mixed and 0.1 ml ferric
chloride were added, immediately followed by timed addition of 3 ml
of 0.008 M of ferricyanide solution. The absorbance at 720 nm was
read after 10 min standing at room temperature. Distilled water was
used as a the blank. The method exploits an oxidation-reduction
reaction in which the phenolate ion is oxidized. The ferric ions are
reduced to the ferrous state and detected by the formation of the
Prussian Blue complex (Fe4[Fe(CN)6)]3) with a potassium
ferricyanide-containing reagent.
Fatty acid profile determination
Total lipids from plant material were quantitatively extracted, with a
Soxhlet extraction (AOAC, 2005). The extracted fats were stored in
a polytop (glass vial, with a push-in top) under a blanket of nitrogen
and frozen at -20°C, pending analyses.
Approximately 10 mg of extracted lipids were transferred into a
Teflon-lined screw-top test tube by means of a disposable glass
Pasteur pipette. Fatty acid methyl esters (FAME) were prepared for
gas chromatography by methylation of the extracted fat, using
methanol–BF3 (Christie et al., 2001). Fatty acid methyl esters were
quantified using a Varian GX 3400 flame ionization GC, with a
fused silica capillary column, Chrompack CPSIL 88 (100 m length,
3. Moyo et al. 12927
Table 1. Chemical composition of dried leaves of Moringa (M. oleifera Lam.)
Nutritive value Dry leaf Standard error
Moisture (%) 9.533 0.194
Crude protein (%) 30.29 1.480
Fat (%) 6.50 1.042
Ash (%) 7.64 0.433
Neutral detergent fibre (%) 11.40 0.425
Acid detergent fibre (%) 8.49 0.348
Acid detergent lignin (%) 1.8 2.204
Acid detergent cellulose (%) 4.01 0.101
Condensed tannins (mg/g) 3.12 0.104
Total polyphenols (%) 2.02 0.390
0.25 mm ID, 0.2 μm film thickness). Analysis was performed using
an initial isothermic period (40°C for 2 min). Thereafter, the
temperature was increased at a rate of 4°C/min to 230°C. Finally,
an isothermic period of 230°C for 10 min followed. Fatty acid methyl
esters in n-hexane (1 μl) were injected into the column using a
Varian 8200 CX Autosampler with a split ratio of 100:1. The
injection port and detector were both maintained at 250°C.
Hydrogen, at 45 psi, functioned as the carrier gas, while nitrogen
was employed as the makeup gas. Varian Star Chromatography
Software recorded the chromatograms.Fatty acid methyl ester
samples were identified by comparing the retention times of FAME
peaks from samples with those of standards obtained from Supelco
(Supelco 37 Component Fame Mix 47885-U, Sigma-Aldrich Aston
Manor, South Africa). The following fatty acid combinations and
ratios were calculated: total saturated fatty acids (SFA), total mono-unsaturated
fatty acids (MUFA), polyunsaturated fatty acids
(PUFA), PUFA/SFA ratio (P/S) and n-6/n-3 ratio. All other reagents
and solvents were of the analytical grade and obtained from Merck
Chemicals (Pty) Ltd Halfway House. South Africa.
Amino acid determination
The samples were hydrolyzed with 6 M HCl at 100°C for 24 h under
vacuum and amino acids were analyzed using an amino acid
analyser (Bassler and Buchholz, 1993).
Beta-carotene and vitamin E determination
Beta-carotene was measured according to AOAC methods 974.29,
992.04 and 992.06 and the method of Thompson and Duval (1989).
Vitamin E was measured according to the methods of McMurray et
al. (1980), Cort et al. (1983) and Speek et al. (1985) on dried
leaves.
Statistical analyses
Each nutrient analysis was done in triplicate. Data obtained was
processed using SAS proc means (2003) which computed the
means and standard errors.
RESULTS AND DISCUSSION
The dried leaves of Moringa had a CP content of 30.3%
(Table 1) with 19 amino acids (Table 2). The highest
value of the amino acids was alanine, which had a value
of 3.033% and the least content was cysteine with 0.01%.
Calcium had the highest value of 3.65% followed by
pottassium (1.5%) and phosphorus had the least value of
0.30% among the macro-elements (Table 3). The highest
value among the micro-minerals was Fe with 490 mg/kg
followed by Se with 3.63 mg/kg. Copper had the least
value of 8.25 mg/kg (Table 3). The dried Moringa leaves
were found to contain 17 fatty acids and α-linolenic acid
(44.57%) had the highest value followed by
heneicosanoic (14.41%), g-linolenic (0.20%) palmiteic
(0.17%) and capric acid (0.07%) (Table 4). Vitamin E had
the highest level with 77 mg/100 g, while Beta-carotene
had 18.5 mg/100 g. The fiber content been NDF, ADF,
ADL and ADC of the leaves were 11.4, 8.49, 1.8 and
4.01%, respectively. The condensed tannins had a value
of 3.2%, while total polyphenols were 2.02% (Table 1).
The study showed that Moringa leaves contain
nutritious compounds. Noteworthy is the crude protein
content of 30.3% observed in this study, although lower
than sunflower seed cake’s CP of 35.88% which is mostly
used as protein concentrate (Mapiye et al., 2010). This
makes the Moringa leaves to be a good potential source
of supplementary protein in animal diets. Other studies
have reported variable protein contents ranging between
16, 22.42, 23.27, 27.4 and 40% (Gidamis et al., 2003;
Sarwatt et al., 2004; Nouala et al., 2006; Reyes-Sanchez
et al., 2006; Oduro et al., 2008; Sanchez-Machado et al.,
2009). This level of crude protein content is of particular
nutritional significance as it may meet animal’s protein
and energy requirements and boost the immune system
4. 12928 Afr. J. Biotechnol.
Table 2. Amino acids composition of dried Moringa (M. oleifera Lam.)leaves
Amino acid Quantity (mean+/- %) Standard error
Arginine 1.78 0.010
Serine 1.087 0.035
Aspartic acid 1.43 0.045
Glutamic acid 2.53 0.062
Glycine 1.533 0.060
Threonine* 1.357 0.124
Alanine 3.033 0.006
Tyrosine* 2.650 0.015
Proline 1.203 0.006
HO-Proline 0.093 0.006
Methionine* 0.297 0.006
Valine* 1.413 0.021
Phenylalanine* 1.64 0.006
Isoleucine* 1.177 0.006
Leucine* 1.96 0.010
Histidine* 0.716 0.006
Lysine* 1.637 0.006
Cysteine 0.01 0.000
Tryptophan* 0.486 0.001
*General essential amino acids.
against diseases (Kyriazakis and Houdijk, 2006; Brisibe
et al., 2009). General growing ruminants like goats
require 16% CP (Luginbuhl and Poore, 1998). The CP
supplied by Moringa is above the protein of goats making
it ideal for use as a protein supplement.
Moringa is reported to have high quality protein which
is easily digested and that is influenced by the quality of
its amino acids (Foidl et al., 2001). In this study, the dried
Moringa leaves contained 19 amino acids, which slightly
differ from the findings of Foidl et al. (2001) and Sanchez-
Machado et al. (2009) who reported 18 and 16 amino
acids respectively. Only glutamine was not detected from
the common 20 amino acids, however, glutamine can be
derived from glutamic acid (Misner, 2008). Out of the 19
amino acids observed, 10 were classified as essential
amino acids namely threonine, tyrosine, methionine,
valine, phenylalanine, isoleucine, leucine, histadine,
lysine and tryptophan. Alanine had the highest value of
3.03%, which differed with Sanchez-Machado et al.
(2009) who reported the value of 1.25%. In their work,
Sanchez-Machado et al. (2009) reported leucine having
the highest value of 1.75%, which is lower than that of
our findings (1.96%). Findings from this study showed the
presence of HO-proline, cystine and tryptophan which
was detected in Sanchez-Machado et al. (2009) work.
Cystine and HO-proline had the least values followed by
methionine, which is commonly deficient in green leaves.
Methionine and cystine are powerful antioxidants that
help in the detoxification of harmful compounds and
protect the body from radiation (Brisibe et al., 2009). HO-proline
is a major component of the protein collagen; it
plays a key role in collagen stability. The variations in the
amino acid composition could be influenced by protein
quality and the origin of the plant (cultivated or wild). This
may indicate that the Moringa was grown in fertile soils.
Usually cultivated plants are fertilized, which could
influence the quality of proteins (Sanchez-Machado et al.,
2009).
Amino acids are organic compounds that combine to
form proteins; as such, they influence the quantity and
quality of protein. Amino acids are classified as essential
and non-essential, which vary according to animal
species and their production system (Swanepoel et al.,
2010). Rumen microbes synthesize the essential amino
acids from other amino acids or from nitrogen containing
substances. The efficiency of rumen microbial growth and
activity in the rumen is enhanced by the presence of
adequate amino acids, peptides and most macro and
micro minerals (Swanepoel et al., 2010). Each amino
acid has a specific function in the animal’s body. In
general, amino acids are required for the production of
enzymes, immunoglobins, hormones, growth, repair of
body tissues and form the structure of red blood cell
(Brisibe et al., 2009). In addition, they contribute to the
formation of glucose, acting as a buffer when other
precursors are in short supply (Swanepoel et al., 2010).
5. Moyo et al. 12929
Table 3. Mineral contents of dried Moringa (M. oleifera Lam.) leaves.
Mineral Dry leaf Standard error
Macro-elements (%)
Calcium % 3.65 0.036
Phosphorus % 0.30 0.004
Magnesium % 0.50 0.005
Potassium % 1.50 0.019
Sodium % 0.164 0.017
Sulphur % 0.63 0.146
Micro-elements (mg/kg)
Zinc (mg/kg) 31.03 3.410
Copper (mg/kg) 8.25 0.143
Manganese (mg/kg) 86.8 3.940
Iron (mg/kg) 490 49.645
Selenium (mg/kg) 363.00 0.413
Boron (mg/kg) 49.93 2.302
Amino acids also affect the function of other nutrients in
the animal’s body such as presence of lysine, which
ensures adequate calcium absorption and aids in the
antibody production.
The dry leaves could serve as a protein supplementary
source in animal and human diets. This protein content is
of particular nutritional significance since it has been
suggested that amino acids supplementation is important
in meeting a substantial proportion of an animal’s protein
and energy requirements (Brisibe et al., 2009). Diets rich
in amino acids help to boost the immune system against
gastro intestinal parasites infestations (Kyriazakis and
Houdijk, 2006). Proteins are also essential for continuous
replenishment of the endogenous protein that is lost due
to infections with gastro-intestinal helminthes (Coop and
Holmes, 1996).
This study identified 17 fatty acids in the dried leaves of
Moringa, of which 11 were classified as the saturated
fatty acids; however, they had lower values.
Henicosanoic had the highest value of 14.41% followed
by palmitic (11.79%) and capric, which had the least
value of 0.07%. Three polyunsaturated fatty acids were
detected namely α-linolenic, linoleic and g-linolenic with
α-linolenic having the highest value of 44.57%. Sanchez-
Machado et al. (2009) reported α-linolenic having a
higher value of 56.87%. Of interest was α-linolenic, which
is an n-3 fatty acid that belongs to the group of the
essential fatty acids. Our findings differ from that of
Sanchez-Machado et al. (2009) who found 14 fatty acids,
which could be attributed to the age of the leaves, soil
type and climatic conditions. Sanchez-Machado et al.
(2009), however, reported that caprylic acid (0.96%),
palmitic acid (3.66%) and arachidonic acid (0.12%) had
the lowest in value, whereas we found that capric,
palmiteic and g-linolenic had the lowest values. Of these
three fatty acids, only lauric was found in our analysis. As
observed in this study, Moringa contains more dietary
polyunsaturated fatty acids than the saturated fatty acids.
A higher content of PUFA and lower amount of SFA is
desirable (Hoffman and Wilklund, 2006), as such, its
inclusion in the diet is recommended as it prevents the
occurrence of diseases thereby promoting good health.
Wood et al. (2008) recommended more consumption of
α-linolenic acid, which promote the endogenous
synthesis of long chain n-3 fatty acids.
Polyunsaturated fatty acids are important for human
and animal health. They are of interest because they are
precursors of long chain n-3 PUFA in the eicosanoids
biosynthesis, which are viewed as important
bioregulators of many cellular processes (Khotimchenko,
2005). They are linked to the development and
functionality of the immune system. Consumers have
preference of food low in saturated fatty acids (SFA)
because they are associated with an increased risk of
cardio-vascular diseases and some cancers (Griffin,
2008; Alfaia et al., 2009). Human nutritionists urge
consumers to increase intake of polyunsaturated fatty
acids (PUFA), particularly the n-3 PUFA at the expense
of n-6 PUFA (Hoffman and Wiklund, 2006; Alfaia et al.,
2009). The quantity and composition of fatty acids in the
animals’ body are related to the presence of some of their
precursors in the diet, since some of the fatty acids are
absorbed in the body unchanged (Wood et al., 2003).
The observed low concentration of acid detergent fibres
and neutral detergent fibres in the study compared with
most forage plants is of interest because, fibre fraction
6. 12930 Afr. J. Biotechnol.
Table 4. Fatty acids composition of dried Moringa (M. oleifera Lam.) leaves.
Fatty acid Quantity (mean+/- %) Standard error
Ether extract 6.50 0.041
Capric (C10:0) 0.07 0.064
Lauric (C12:0) 0.58 0.402
Myritic (C14:0) 3.66 1.633
Palmitic (C16:0) 11.79 0.625
Palmitoleic (C16:1c9) 0.17 0.056
Margaric (C17:0) 3.19 0.155
Stearic acid (C18:0) 2.13 0.406
Oleic (C18:1c9) 3.96 2.000
Vaccenic (C18:1c7) 0.36 0.038
Linoleic (C18;2c9,12(n-6) 7.44 0.014
α-Linolenic (C18:3c9,12,15(n-3) 44.57 2.803
g-Linolenic (C18:3c6,9,12 (n-6) 0.20 0.013
Arachidic (C20:0) 1.61 0.105
Heneicosanoic (C21:0) 14.41 0.194
Behenic (C22:0) 1.24 0.383
Tricosanoic (C23:0) 0.66 0.025
Lignoceric (24:0) 2.91 0.000
Total saturated fatty acids (SFA) 43.31 0.815
Total mono unsaturated fatty acids (MUFA) 4.48 1.984
Total poly unsaturated fatty acids (PUFA) 52.21 2.792
Total Omega-6 fatty acids (n-6) 7.64 0.012
Total Omega-3 fatty acids (n-3) 44.57 2.805
PUFA: SFA (PUFA:SFA) 1.21 0.096
n-6/n-3 0.17 0.016
PUFA: MUFA (PUFA:MUFA) 14.80 7.168
defines the extent and rate of feed digestibility (Rubanza
et al., 2005). The values of NDF and ADF of 11.4 and
8.49% differed from that of the findings of Foidl et al.
(2001) that showed NDF and ADF values of 21.9 and
11.4%, respectively, suggesting that the leaves used in
this study were of high digestibility. These variations of
NDF and ADF values may be due to differences in agro-climatic
conditions, age of the trees and possibly due to
different stages of maturity of leaves. The observed
concentrations of acid detergent lignin (ADL) in this study
were however, consistent with values reported by Foidl et
al. (2001).
Another interesting aspect of the results reported here
is the low percentages of anti-nutritional factors in the
leaves, which though present were negligible. The value
of condensed tannins was 3.12%, while Foidl et al.
(2001) reported 1.4% of tannins and did not detect the
condensed tannins. Drying is reported to reduce or
remove extractable condensed tannins by 15 to 30%
relative to fresh foliage (Vitti et al., 2005). The decrease
of condensed tannins after drying may be due to
decomplexation between tannins and proteins and
depolymerisation and oxidation of tannins (Makkar,
2003). The content of total phenols (2.02%) in this study
was lower than previously reported values of 2.7 and
4.3% (Gupta et al., 1989; Foidl et al., 2001). At these
concentrations, simple phenols do not produce any
adverse effects when consumed by animals (Foidl et al.,
2001). However, these phenols have been reported to
have multiple beneficial biological effects that include
antioxidant activity, anti-inflammatory action, inhibition of
platelet aggregation, antimicrobial activities and antitumor
activities (Thurber and Fahey, 2009)
It is also of remarkable interest that the dried Moringa
leaves have high deposit of mineral elements. Calcium
was observed to be higher compared with other plant
sources (Nkafamiya et al., 2010). It is required for
formation and maintenance of bones and teeth thus,
preventing osteoporosis. It is also needed for normal
blood clotting and nervous function. Interestingly, even
7. Fe, which is commonly deficient in many plant-based
diets, was found in abundance in this plant’s leaves. Iron
is a necessary component of haemoglobin and myoglobin
for oxygen transport and cellular processes of growth and
division (Kozat, 2007). Iron is also an essential trace
element for normal functioning of the central nervous
system and in the oxidation of carbohydrates, proteins
and fats (Umar et al., 2007). Iron also has a role in ener-gy
metabolism as it facilitates transfer of electrons in the
electron transport chain for the formation of ATP (Kozat,
2007).
The presence of Zn in high amounts is of special
interest in view of the importance of the inclusion of Zn in
the diet of animals and humans. Results from this study
had higher levels of zinc (31.03 mg/kg) than the findings
of Barminas et al. (1998) who reported 25.5 mg/kg in
dried Moringa leaves. Zinc is essential for the synthesis
of DNA, RNA, insulin and function and/or structure of
several enzymes (Brisibe et al., 2009). Zinc is also
required for cell reproduction and growth especially
sperm cells. In addition, Zn is known for its anti-viral, anti-bacterial,
anti-fungal and anti-cancer properties (Brisibe
et al., 2009).
The Moringa dried leaves contained Cu, which is
considered to have strong effects on the immune
system (Anwar et al., 2007). Copper is involved in
stimulating body defence system, as it is active in
neutrophil production and affects phagocyte killing ability.
It is required for antibody development and lymphocyte
replication (Burke and Miller, 2006). Copper in combi-nation
with Zn, plays a role in superoxide dismutase
activity and the removal of oxygen free radicals. It is
therefore, a key component in the protective mechanism
of cellular membranes against superoxide free radicals
damage (Guo et al., 2010). In addition, the copper
containing enzyme, ceruloplasmin has been shown to
exhibit antinflammatory activity, which may prove
beneficial in mastitis cases (Guo et al., 2010). Copper
has been found to reduce internal parasite namely
Haemonchus contortus load in sheep and goat (Burke
and Miller, 2006). Moringa has sulphur that is necessary
for efficiency of rumen microbial growth and activity
(Brisibe et al., 2009). Moringa mineral composition plays
a significant role in nutritional, medicinal and thera-peutic
values (Al-kharusi et al., 2009).
The results showed that the dried powdered Moringa
leaves have high levels of vitamin E and βeta-carotene.
Moringa powder has been reported to be rich in beta-carotene,
thiamine, riboflavin, niacin, pyrodixine, biotin,
ascorbic acid, cholecalciferol, tocopherol and vitamin K
(Broin, 2006). As such in our study, we investigated the
presence of beta-carotene and vitamin E in the dried
leaves. The reason been that under normal conditions,
healthy ruminants synthesize adequate amounts of B
vitamins as well as vitamin C and K (Rinehart, 2008).
Moyo et al. 12931
Beta-carotene is the most potent precursor to vitamin A.
The animals are able to convert beta-carotene into
vitamin A within their body (Panday and Tiwari, 2002).
Moringa is reported to be rich in vitamin C which
increases iron absorption in the animal’s body (Anwar et
al., 2007). Vitamin A is necessary for many functions in
the ruminants including vision, bone growth, immunity
and maintenance of epithelial tissue. In addition, vitamin
A also maintains adequate levels of iron in plasma that
supply the different body tissues including the bone
marrow (Thurber and Fahey, 2009). Supplementation of
diets with both iron and vitamin A may increase the iron
status as measured by haemotological indices like
haemoglobin and haemocrit (Babu, 2000).
Beta-carotene rich Moringa leaves can thus be an
important source of vitamin A, can be used for releasing
the bound iron status and thus, help in reducing anaemia
as well as prevalence of vitamin A deficiency. Vitamin A
and E are some of the specific nutrients that assist
animals to develop disease resistance. Our findings are
in agreement with that of Fuglie (2001), where the
amount of vitamin was 113 mg/100 g of the dried leaves.
Vitamin E is known to help maintain and increase the
storage of vitamin A and iron in the body. Moringa powder
is, however, rich in vitamin such that it is one of the
richest plant sources of vitamin (Anwar et al., 2007).
Vitamin E with selenium contains antioxidant that work
co-dependently in the body to help destroy free radicals
(Rock et al., 2001). The interaction of selenium and
immune function focuses around the selenoprotein, gluta-hione
peroxidase. Gluthione peroxidase inactivates
oxygen radicals such as hydrogen peroxide and prevents
them causing cellular damage. Also, supplementing dairy
cattle with adequate levels of selenium (0.3 ppm of dry
mater) reduce the prevalence, severity and duration of
mastitis (Rock et al., 2001). Looking at all the properties
of the plant leaves, this probably explains the traditional
use of the plant as an herbal tonic in India, because of its
high levels of readily available essential nutrients and
mineral resources, which may be required for the
maintenance of electrical potential of nervous tissues and
cell membranes. It can as well be used for the treatment
of blood related disorders that is necessary for the
improvement of the overall well-being of the body
(Khalafalla et al., 2010).
The nutritional variations observed among the studies
could be attributed to the genetic background of the plant,
in terms of ecotype and cultivar, environmental factors
that include the soil and climate (Sanchez-Machado et
al., 2009). In addition, the cultivation method used
encompasses the frequency of harvesting and age of the
plant or leaves. Mode of conservation between collection
and analysis (drying, refridgeration, freezing) might
influence the leaves’ nutritional composition (Barminas et
al., 1998; Broin, 2006).
8. 12932 Afr. J. Biotechnol.
In conclusion, the data derived from nutrient
characterization of Moringa are clear indications that the
plant leaves are rich in nutrients and has potential to be
used as a feed additive with multiple purposes. These
include serving as a protein, fatty acid, mineral and
vitamin resource for animal and human feed formulations.
High nutritional content found in the dried leaves are
important nutritional indicators of the usefulness of the
plant as a likely feed resource. Drying the leaves assists
to concentrate the nutrients, facilitate conservation and
consumption, as such, it can be used during the time
when feed is scarce or can be transported to areas where
it is not cultivated. It is suggested that Moringa should be
consumed in the powder form. Moringa has been
reported to possess some medicinal properties (Fahey,
2005); its inclusion in the diets could function as curative
and therapeutic therapy. As such, it can be used to
improve health and nutrition in sub-Saharan countries.
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Moringa Miracle Tree