1) The study reviewed the documented phytochemistry of Vernonia amygdalina leaves, which revealed the presence of compounds like anthraquinones, tannins, flavonoids, alkaloids, saponins, and terpenoids.
2) Previous studies on the pharmacologic activities of V. amygdalina extract found it has hypoglycemic, antimalarial, hypolipidemic, anti-inflammatory, and antioxidant effects. These activities are attributed to the phytochemicals present in the plant.
3) One study found V. amygdalina extracts had significant antioxidant activity in various test systems due to their total phenolic and flavonoid
n Ayurveda, withania is widely claimed to have potent aphrodisiac, sedative, rejuvenative and life prolonging properties. It is also used as a general energy-enhancing tonic known as Medharasayana, which means 'that which promotes learning and a good memory' and in geriatric problems.
n Ayurveda, withania is widely claimed to have potent aphrodisiac, sedative, rejuvenative and life prolonging properties. It is also used as a general energy-enhancing tonic known as Medharasayana, which means 'that which promotes learning and a good memory' and in geriatric problems.
Ethnobotany and Ethnopharmacology-Approaches of Traditional Medicine Studies, Traditional use & management of medicinal plants in Asian countries, Application of Ethnobotany to community conservation and medicinal plant resource management
Phytochemical, Antioxidant and Antibacterial Studies on Bambusa arundinacea a...SUS GROUP OF INSTITUTIONS
This study was formulated to check the phytochemical, antioxidant, antibacterial potential of
Bambusa arundinacea (Bamboo) and Mangifera indica (Mango) trees. Aqueous, ethanolic and
methanolic extracts were prepared from leaves of former and stem bark of later. The phytochemical
screening of the extracts showed the presence of various bioactive compounds such as
carbohydrates, flavonoids, saponins and proteins in B. arundinacea, alkaloids, flavonoids, tannins,
saponins, steroids and cardiac glycosides in M. indica. Total phenolic concentration and
percentage of free radical scavenging activity was more in ethanolic extracts of B. arundinacea and
M. indica followed by methanolic extracts and aqueous extracts. Highest percentage of ferric
reducing antioxidant power was found in ethanolic extracts and lowest in aqueous extracts indicates
that ethanolic extracts has more antioxidant potential than the other two extracts. Ethanolic extracts
of both plants had higher inhibition on the tested Gram positive (B. subtilis & S. aureus) as well as
Gram negative (E.coli & P. aeruginosa) bacteria evidenced from the zones of inhibition. M. indica
showed more therapeutic potential as compared to B. arundinacea and ethanolic as well as
methanolic extracts of both the tested plants were more effective than aqueous extracts due to better
extraction power of organic solvents. Overall study indicates that B. arundinacea and M. indica are
potential source of natural antioxidants, phytochemicals and antibacterials that can be used for the
development of novel drugs and may represent new source of antimicrobials with stable, biologically
active components that can establish a scientific base for further use in modern medicines.
Herbal Drugs and its Plant Sources (Stoechas Linn, Adhatoda (Acanthaceae), Ammi (Apiaceae), Bacopa (Scrophulariaceae), Cedrus (Pinaceae), Diospyros (Meliaceae), Hibiscus (Malraceas), Ophiorhiza (Rubiaceae), Sesbania (Papilionaceae), Trifolium (Papilionaceae), Zea (Poaceae), Xanthium (Asteraceae), Vicia (Papilionaceae))
India is a large repository of herbal drugs and become a big centre and fountain in the field of herbal therapy and beautification. Treatment through the herbs is very popular in the ancient time in Indian subcontinent and now approaching to new boundaries. Plants had been used for medicinal purposes long before recorded history. It is becoming more main stream as improvements in analysis and quality control along with advances in clinical research show the value of herbal medicine in the treating and preventing disease. Herbal medicine is used to treat many conditions, such as asthma, eczema, migraine, etc. Medicinal plants have been used as a major source of therapeutic agents by human being for thousands of years.
See more
https://goo.gl/dMaVYF
https://goo.gl/HdATS6
https://goo.gl/GqCSBU
Contact us:
Niir Project Consultancy Services
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Herbal Drugs, List of Plants Used in Herbalism, Indian Herbs and Herbal Drugs, Plant Based Drugs and Medicines, Herbal Plants and their uses, Medicinal Plants in India, Medicinal Plants and Herbal Remedies, Medicinal Plants and Herbs, Herbal Medicine Plants, Herbal Medicinal Products, Ayurvedic, Herbal Drugs and Medicinal Plants, Herbal Drug from Natural Sources, Sources of Herbal Medicine, Plants as Source of Herbal Drugs, Drug from Medicinal Plants, Filariasis, Stoechas Linn, Herbal Preparations, Abroma (Sterculiacease), Acer (Aceraceae), Allamanda (Apocynaceae), Aloysia (Verbenaceae), Berberis (Berberidaceae), Brucea (Simaroubaceae), Capparis (Capparaceae), Centaurea (Asterceae), Cinnamomum (Cephalotoxaceae), Crotalaria (Papilionaceae), Dauncus (Apiaceae), Ervatamia (Apecynaceae), Fritillaria (Liliaceae), Erythroxylum (Erythroxylaceae), Ferula (Apiaceae), Gardenia (Rubiaceae), Glycyrrhiza (Papilianaceae), Helleborus (Ranunculaceae), Hypericum (Hypericaceae), Ipomoea (Convolvulaceae), Iberis (Brassicaceae), Lantana (Verbenaceae), Jatropha (Euphorbiaceae), Madhuca (Sapotaceae), Melilotus (Papilionaceae), Morus (Moraceae), Nelumbium (Nymphaeaceae), Nicotiana (Solanaceae), Nothapodytes (Icacinaceae), Ophiorhiza (Rubiaceae), Panax (Araliaceae), Parthenium (Asteraceae), Rosmarinus (Lamiaceae), Saccharum (Poaceae), Salvadora (Salvadraceae), Samadera (Simaroubaceae), Selinum (Apiaceae), Tiliacora (Menispermaceae), Toona (Meliaceae), Valeriana (Valerianaceae), Wikstroemia (Thymelaeaceae),
Nutraceuticals chapter of Advance Pharmacognosy 1 of M Pharm syllabus.
This presentation involves Introduction to Nutraceuticals,
Classification of Nutraceuticals, Herbs as a food, Inorganic /mineral supplements, brief benefits of vitamin supplements, digestive enzymes and its example, use of cereals and Grains, importance of Antioxidants and Polyunsaturated fatty acids, an example of formulation and standardization of Multivitamin Tablets, what all regulatory requirement we need to manufacture Nutraceuticals and FSSAI guidelines for Nutraceuticals, sources- mediinal use - marker compound of some usually used Nutraceuticals.
Phytochemical and anti proliferative activity of Nigella sativa (Kalonji)Saptarshi Samajdar
Highly potent plant having very useful pharmacological effect especially in anticancer studies and various other diseases. It is even called Panacea because it can cure all diseases except death. Various religious books mention its existence as potent herbal drug.
Ethnobotany and Ethnopharmacology-Approaches of Traditional Medicine Studies, Traditional use & management of medicinal plants in Asian countries, Application of Ethnobotany to community conservation and medicinal plant resource management
Phytochemical, Antioxidant and Antibacterial Studies on Bambusa arundinacea a...SUS GROUP OF INSTITUTIONS
This study was formulated to check the phytochemical, antioxidant, antibacterial potential of
Bambusa arundinacea (Bamboo) and Mangifera indica (Mango) trees. Aqueous, ethanolic and
methanolic extracts were prepared from leaves of former and stem bark of later. The phytochemical
screening of the extracts showed the presence of various bioactive compounds such as
carbohydrates, flavonoids, saponins and proteins in B. arundinacea, alkaloids, flavonoids, tannins,
saponins, steroids and cardiac glycosides in M. indica. Total phenolic concentration and
percentage of free radical scavenging activity was more in ethanolic extracts of B. arundinacea and
M. indica followed by methanolic extracts and aqueous extracts. Highest percentage of ferric
reducing antioxidant power was found in ethanolic extracts and lowest in aqueous extracts indicates
that ethanolic extracts has more antioxidant potential than the other two extracts. Ethanolic extracts
of both plants had higher inhibition on the tested Gram positive (B. subtilis & S. aureus) as well as
Gram negative (E.coli & P. aeruginosa) bacteria evidenced from the zones of inhibition. M. indica
showed more therapeutic potential as compared to B. arundinacea and ethanolic as well as
methanolic extracts of both the tested plants were more effective than aqueous extracts due to better
extraction power of organic solvents. Overall study indicates that B. arundinacea and M. indica are
potential source of natural antioxidants, phytochemicals and antibacterials that can be used for the
development of novel drugs and may represent new source of antimicrobials with stable, biologically
active components that can establish a scientific base for further use in modern medicines.
Herbal Drugs and its Plant Sources (Stoechas Linn, Adhatoda (Acanthaceae), Ammi (Apiaceae), Bacopa (Scrophulariaceae), Cedrus (Pinaceae), Diospyros (Meliaceae), Hibiscus (Malraceas), Ophiorhiza (Rubiaceae), Sesbania (Papilionaceae), Trifolium (Papilionaceae), Zea (Poaceae), Xanthium (Asteraceae), Vicia (Papilionaceae))
India is a large repository of herbal drugs and become a big centre and fountain in the field of herbal therapy and beautification. Treatment through the herbs is very popular in the ancient time in Indian subcontinent and now approaching to new boundaries. Plants had been used for medicinal purposes long before recorded history. It is becoming more main stream as improvements in analysis and quality control along with advances in clinical research show the value of herbal medicine in the treating and preventing disease. Herbal medicine is used to treat many conditions, such as asthma, eczema, migraine, etc. Medicinal plants have been used as a major source of therapeutic agents by human being for thousands of years.
See more
https://goo.gl/dMaVYF
https://goo.gl/HdATS6
https://goo.gl/GqCSBU
Contact us:
Niir Project Consultancy Services
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Herbal Drugs, List of Plants Used in Herbalism, Indian Herbs and Herbal Drugs, Plant Based Drugs and Medicines, Herbal Plants and their uses, Medicinal Plants in India, Medicinal Plants and Herbal Remedies, Medicinal Plants and Herbs, Herbal Medicine Plants, Herbal Medicinal Products, Ayurvedic, Herbal Drugs and Medicinal Plants, Herbal Drug from Natural Sources, Sources of Herbal Medicine, Plants as Source of Herbal Drugs, Drug from Medicinal Plants, Filariasis, Stoechas Linn, Herbal Preparations, Abroma (Sterculiacease), Acer (Aceraceae), Allamanda (Apocynaceae), Aloysia (Verbenaceae), Berberis (Berberidaceae), Brucea (Simaroubaceae), Capparis (Capparaceae), Centaurea (Asterceae), Cinnamomum (Cephalotoxaceae), Crotalaria (Papilionaceae), Dauncus (Apiaceae), Ervatamia (Apecynaceae), Fritillaria (Liliaceae), Erythroxylum (Erythroxylaceae), Ferula (Apiaceae), Gardenia (Rubiaceae), Glycyrrhiza (Papilianaceae), Helleborus (Ranunculaceae), Hypericum (Hypericaceae), Ipomoea (Convolvulaceae), Iberis (Brassicaceae), Lantana (Verbenaceae), Jatropha (Euphorbiaceae), Madhuca (Sapotaceae), Melilotus (Papilionaceae), Morus (Moraceae), Nelumbium (Nymphaeaceae), Nicotiana (Solanaceae), Nothapodytes (Icacinaceae), Ophiorhiza (Rubiaceae), Panax (Araliaceae), Parthenium (Asteraceae), Rosmarinus (Lamiaceae), Saccharum (Poaceae), Salvadora (Salvadraceae), Samadera (Simaroubaceae), Selinum (Apiaceae), Tiliacora (Menispermaceae), Toona (Meliaceae), Valeriana (Valerianaceae), Wikstroemia (Thymelaeaceae),
Nutraceuticals chapter of Advance Pharmacognosy 1 of M Pharm syllabus.
This presentation involves Introduction to Nutraceuticals,
Classification of Nutraceuticals, Herbs as a food, Inorganic /mineral supplements, brief benefits of vitamin supplements, digestive enzymes and its example, use of cereals and Grains, importance of Antioxidants and Polyunsaturated fatty acids, an example of formulation and standardization of Multivitamin Tablets, what all regulatory requirement we need to manufacture Nutraceuticals and FSSAI guidelines for Nutraceuticals, sources- mediinal use - marker compound of some usually used Nutraceuticals.
Phytochemical and anti proliferative activity of Nigella sativa (Kalonji)Saptarshi Samajdar
Highly potent plant having very useful pharmacological effect especially in anticancer studies and various other diseases. It is even called Panacea because it can cure all diseases except death. Various religious books mention its existence as potent herbal drug.
Effect of Different Processing Methods on Nutritional Composition of Bitter L...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Toxicity of Ocimum gratissimum leaf extract on the developmental stages of ...researchanimalsciences
The effect of some plant extract on the developmental stages of mosquito
species was conducted using
Anopheles gambiae
,
Culex quinquefasciatus
and
Aedes aegypti
at the first instar, second instar, third instar, fourth instar, pupae and
adult. The plant used was scentleaf (
Ocimum gratissimum
). Soxhlet apparatus was
used to extract each pulverized plant part using petroleum ether solvent. Plant
extract from the petroleum ether solvent was used to test their effects on the
developmental stages of each mosquito species. The result showed that the higher
the developmental stages, the lower the percentage of mortality. It was also
observed that as the time and concentration increases, the percentage of mortality
also increases. Generally, the result showed that
Anopheles gambiae
is more
susceptible in the extracts used followed by
Aedes aegypti
and then
Culex
quinquefasciatus
.
ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
Key-words- Medicinal plants, Antimicrobial activity, Antifungal activity
We are prime manufacturers of BioPesticide/Fertilizer/Fungicide,Plant Growth Regulator,Agri Enzymes & Beneficiary Microbial Formulations manufactured using World's Latest SSF Technology.
Antioxidant properties of some leafy and non leafy vegetables in west africaAkinbobola Akinwemoye
This is a seminar report on the Antioxidant properties of some leafy and non leafy vegetables in west africa. Get my contact on http://www.livestocking.com/p/contact-us.html to get a copy of this.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Pharmacognosy-III
If you seeking data about "herb as health food ",this assignment will be helpful to you and provide more and more information about different medicinal plant with their sourse, constituent, uses and for easily identification figure were added and mentioned shortly rules of medicinal plant in public health and economy .So hope that assignment willbe beneficial for everyone. Please forgive my mistake .
Terminalia avicennioides is an important medicinal plant used to manage many pathological
conditions among most inhabitants of rural communities in Nigeria and many other African countries. It
belongs to family Combretaceae and found commonly growing in the savannah region of West Africa. The
parts of the plant mostly used for medicinal purpose are root and stem barks extracts, which are rich sources
of phytochemicals such as anthraquinone, saponins, steroids, tannins and terpenes among others. In folk
medicine, there are claims that the plant has been used to cure various diseases such as dental caries, skin
infections, sore and ulcer, syphilis, bloody sputum, ringworm infection, gastrointestinal helminthes and several
others. In this review, we have tried to provide bases for most of these claims at the instance of the results of
modern investigations on the biological activities of the plant extract. The possibility of employing the plant
extract in the field of green chemistry for probable application in biomedical industries was also discussed.
Keywords: Terminalia avicennioides, uses, phytochemicals, biological activities, nanoparticles
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
PHYTO-NUTRITIONAL PROFILES OF BROOM WEED (SIDA ACUTA) LEAF EXTRACTSubmissionResearchpa
Medicinal plants are abundant in phytochemicals that has significant therapeutic effects. They are relatively cheap, effective and safe in prolong use. This experiment was carried out to investigate the phyto-nutritional profile of broom weed (Sida acuta) leaf extract. Proximate analysis Sida acuta leaf indicated the presence of dry matter (91.88 %), crude protein (18.01 %), ash (9.73 %), crude fibre (6.24 %), ether extract (1.77 %) and energy (2760 Kcal/kg). Phytochemical screening of the extract revealed the presence of condensed tannins, hydrolysable tannins, flavonoids, saponins, phenols, oxalate, phytate, alkaloids, terpenoids and glycosides at 0.82 %, 2.02 %, 4.25 %, 0.20 %, 0.17 %, 0.05 %, 0.23 %, 0.22 %, 0.95 % and 0.02 % respectively and the vitamin constituents are thiamine (0.33 mg/100g), ascorbic acid (30.17 mg/100g), riboflavin (0.05 mg/100g), β-carotene (0.79 mg/100g) and niacin (0.41 mg/100g). Mineral analysis showed that it contained calcium (127.6 mg/100g), phosphorus (78.6 mg/100g), potassium (31.6 mg/100g), magnesium (102.1 mg/100g), iron (2.14 mg/100g), manganese (0.60 mg/100g), copper (0.04 mg/100g) and zinc (1.75 mg/100g). Result on amino acid composition showed that they contained lysine (2.11 mg/100g), arginine (1.77 mg/100g), aspartic acid (2.13 mg/100g), threonine (1.85 mg/100g), histidine (4.71 mg/100g), serine (2.03 mg/100g), glycine (1.05 mg/100g), alanine (3.31 mg/100g), cystine (5.06 mg/100g), valine (0.88 mg/100g), leucine (2.04 mg/100g), phenyalanine (4.72 mg/100g), tyrosine (3.51 mg/100g), isoleucine (2.84 mg/100g), methionine (0.85 mg/100g) and proline (1.05 mg/100g). It was concluded that Sida acuta leaf extract is rich in various nutrients and phytochemicals conferring it the ability to perform multiple biological activities and as a natural alternative to antibiotics. by Shittu, M. D and Alagbe, J.O 2020. PHYTO-NUTRITIONAL PROFILES OF BROOM WEED (SIDA ACUTA) LEAF EXTRACT. International Journal on Integrated Education. 3, 11 (Nov. 2020), 119-124. DOI:https://doi.org/10.31149/ijie.v3i11.845. https://journals.researchparks.org/index.php/IJIE/article/view/845/806 https://journals.researchparks.org/index.php/IJIE/article/view/845
Is is a project proposal by which the antimicrobial and antioxidant property of Phyllanthus acidus will be explored that will open a new door for the innovation of new medicine.
Phytochemical studies on selected medicinal plant Gymnema sylvestre.researchplantsciences
Gymnema sylvestre R. (Br).(Asclepiadaceae) is a common medicinal plant available in Tamil Nadu is a woody, climbing plant that grows in the tropical forest of central and Southern India. It also happens to be a first-rate warrior against diabetes. The plant is called Gud-mar (Gud-Jaggery, mar-kills) in India and well known for masking sweet taste. It is reported to be effective against many chronic diseases is screened for its phytochemical content, microbial activity and anti-inflammatory activity. Extracts (Alcoholic, aqueous, acetone and hexane) from the plant is prepared and analyzed. Qualitative phytochemical tests are done to detect the presence of Carbohydrate, Alkaloid, Tannins, Phenols, Saponins, Fixed oils, Gums and Mucilage. Quantitative methods like phytochemical analysis, gravimetric estimation, RBC membrane stabilization and TLC profiles are used to determine the active principle Gymnemic acid, anti-inflammatory activity and antimicrobial activity. The results showed that the leaf extracts studied contain the bio active compounds phenols, alkaloids, tannins, saponins and Gymnemic acid. Water extract of normal var. had positive reaction for carbohydrate (Molisch reagent) hairy var. leaves had negative reaction. The presence of active phytochemical substances with antimicrobial and anti-inflammatory activities may provide substantial basis for the use of this plant in ethno medicine.
Article Citation:
Gnana Sangeetha D and Jegadeesan M.
Phytochemical studies on selected medicinal plant Gymnema sylvestre.
Journal of Research in Plant Sciences (2012) 1(1): 077-082.
Full Text:
http://plantsciences.co.in/documents/PS0027.pdf
Antibacterial activity of naturally obtained plant that gives a antibacterial activity and some anti malarial activity functions also.This plant majorly seen in hills areas.
A laboratory bioassay was conducted to investigate the antifeedant effect of Gomphrena serrata extracts on
sitophilus oryzae (rice weevil) belongs to the family Curculionidae. Antifeedants are natural or synthetic
compounds that stops or inhibits feeding by a pest and especially an insect. Gomphrena serrata- Amaranthacae
family comprises many species which are used in nutrition and traditional folk medicine. Study was done to
find the new active substance in the plant which could show antifeedant activity and compared with standard
Strychnos nuxvomica. The extracts of both sample and standard were obtained by cold maceration process. The
residue formed is collected and both the extracts were subjected to study the antifeedant activity. The activity is
performed by dilution method and found to be showing the antifeedant activity. The primary objective of our
work is simple and cost effective method to find out the active substance from natural resources.
Indiscriminate use of synthetic insecticides has led to problems such as the resurgence of primary pests,
secondary pest’s outbreak, resistance development, insecticide residue, health hazards, environmental
contamination and increased cost of insect control. So this study will be solution for these problems by utilizing
plant’s bioactive molecules. Plants are the most efficient producers of phytochemicals in the environment,
including secondary metabolites that are used by the plant in defence against insects. The secondary metabolites
produced from Gomphrena serrata could be utilized in the development of new biopesticides
The Moringa tree (Moringa Oleifera), is the only genus in the family Moringaceae. This plant also known as “Drumstick Tree“, is a tree which essentially grew in the Himalayan region of northern India, however is now cultivated in the Pacific and Caribbean Islands, Africa, Malaysia, Pakistan, Central and South America. Different parts of this plant such as the leaves, seed, bark, roots, flowers, fruit, and immature pods work as heart and circulatory stimulants, possess antitumor, antioxidant, antidiabetic, anti inflammatory, hepatoprotective, antihypertensive, antispasmodic, antifungal and antibacterial effects, and are being utilized for the therapy of numerous diseases.
Similar to A study review of documented phytochemistry of vernonia amygdalina as the basis for pharmacologic activity of plant extract (20)
A study to assess the knowledge regarding prevention of pneumonia among middl...
A study review of documented phytochemistry of vernonia amygdalina as the basis for pharmacologic activity of plant extract
1. Journal of Natural Sciences Research www.iiste.org
ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online)
0921
Vol.2, No.7, 2012
A Study Review of Documented Phytochemistry of Vernonia
amygdalina (Family Asteraceae) as the Basis for Pharmacologic
Activity of Plant Extract
Sani Ali Audu1 * Alemika Emmanuel Taiwo2 Abdulraheem Rafat Ojuolape3
Abdulkareem Sikirat Sani4 Abdulraheem Ramat Bukola5. Ilyas Mohammed5
1.Department of Pharmaceutical Chemistry, Faculty of Pharmacy University of Maiduguri , PMB 1069
Department
Maiduguri, Nigeria aliaudusani@gmail.com
2. Department of Pharmaceutical and Medicinal Chemistry, University of Jos, Jos, Nigeria
alemikat3@gmail.com
3. Department of Food Science and Technology, University of Maiduguri , Nigeria
rafatabdulraheem@yahoo.com
4. Department of Chemistry, Federal Polytechnic, Damaturu, Yobe State, Nigeria
sikirat_kareem@yahoo.com
5.Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Za
Department Zaria, Nigeria
ilyasmn@myway.com>
*aliaudusani@gmail.com+234(0)8035890984 ; +234(0)8055297698
aliaudusani@gmail.com+234(0)8035890984
Abstract
This study was conducted to review phytochemistry of Vernonia amygdalina leaves as a basis for documented
pharmacologic activity of the extract of Vernonia amygdalina. The documented phytochemical screening studies
reveals the presence of Phytochemical screening of various solvent (aqueous, methanol, acetone and n
n-hexane)
extract of Vernonia amygdalina has shown the presence of Anthraquinones (+++), Soluble tannins (++),
Condensed tannins (+), Flavonoids (+), Alkaloids (+++), Indole alkaloids (+), Steroidal alkaloids (+++),
Saponins (+++), Glycosides (++), Cyanogenic glycosides ( ), Terpenoids (+++). Studies conducted to determine
(-),
or/and ascertain the pharmacologic activities of Vernonia amygdalina extract has revealed that these
aforementioned phytochemicals are responsible for the majority of these observed pharmacologic activities
including but not limited to hypoglycaemic, antimalarial, hypolipide
hypolipidemic, anti-inflammatory, anti oxidant
inflammatory,
activities.
Keyword: Vernonia amygdalina Phytochemistry, Leave extract
Introduction
Medicinal plants are various plants thought by some to have medicinal properties, but few plants or their
phytochemical constituents have been proven by rigorous science or approved by regulatory agencies such as the
United States Food and Drug Administration or European Food Safety Authority to have medicinal effects.
Administration
A medicinal plant is any plant which, in one or more of its organs, contains substances that can be used for
the therapeutic purposes or which are precursors for the synthesis of useful drug 20
drugs
A herbal remedy is one in which the main therapeutic activity depends upon the plant or fungal metabolites
which it contains. Some plants are purely dietary and are necessary for health (fresh vegetables, carrots, fruits,
which we now know provide essential vitamins). Many plant products are consumed in reasonable quantity as
ntial
food but known to have medicinal effects (e.g., figs, prunes and mucilage acting as mild laxatives). There are
some purely medicinal plants, few apparently quite safe and others more potent (e.g. containing cardioactive
more
glycosides), which can only be consumed in small quantities but which as such dosage are suitable for the
treatments of disease16.
Preliminary Review of some Literatures
Scientific Classification of Vernonia amygdal
amygdalina
Kingdom: plantae
Division: Angiosperms
Order : Asterales
Family: Asteraceae
Genius: Vernonia
Species: V. amygdalina
Botanical Name: Vernonia amygdalina
Synonyms, Botanical source and habitat
Vernonia amygdalina, a member of the Asteraceae family, is a shrub or small tree of 2 – 5 m with petiolate
,
leaf of about 6 mm diameter and elliptic shape. The leaves are green with a characteristic odour and a bitter taste.
No seeds are produced and the tree has therefore to be distributed through cutt 3. It is known in Nigerian local
cutting
languages as etidot (Efik), uzi (Ebira), onugbu (Igbo) and chusar duki (Hausa). Elsewhere in Africa, it is called
muop or ndole (Cameroon), tuntwano (Tanzania), and mululuza (Uganda) 14.
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Ecology Grows under a range of ecological zones in Africa and produces large mass of forage and is drought
ecological
tolerant. There are about 200 species of Vernonia.
Major uses and functions The leaves are used for human consumption and washed before eating to get rid
of the bitter taste. They are used as vegetable and stimulate the digestive system, as well as they reduce fever.
e
Furthermore, are they used as local medicine against leech, which are transmitting bilharziose. Free living
chimpanzees eat the leaves, if they have attacked by parasites Vernonia amygdalina is also used, instead of hops
parasites.
to make beer in Nigeria3. Furthermore, is Vernonina amygdalina found in homes in villages as fence post and
pot-herbs. It is also used in the treatment of amoebic dysentery, gastrointestinal disorders, mi
herbs. microbial and parasitic
activities, hepatotoxicities2.
It is very unlikely that a single molecule is responsible for such varied activities; instead multiple molecules,
working alone or in combination with others aforementioned, are much more likely to be responsible for each of
these biological activities.
Feeding value Vernonia amygdalina has been observed to be eaten by goats in Central Zone of Delta State,
Nigeria. However, in general has there been found, that Vernonia amygdalina have an astringent tas which taste,
affects its intake. The bitter taste is due to anti nutritional factors such as alkaloids, saponins, tannins and
anti-nutritional
glycosides2.
It has been tried to mix Vernonia with molasses to make it more palatable, but 6.6 % of DM intake had to
be added to improve the intake of Vernonia. During the dry period Dairy farmers from Southern Ethiopia feed
prove
boiled Vernonia, since the boiling decreases the content of secondary plant compounds and makes the feed more
palatable.
Vernonia amygdalina has also been fed to broilers, where it was able to replace 300 g kg-1 of maize-based
diet without affecting feed intake, body weight gain and feed efficiency2.
Pharmacology The leaf of Vernonia amygdalina extract is used in medicine as a antimalarial, antimicrobial,
laxative, antihelmitic, antithrombotic and both hypoglycemic cum
e, hypolipidaemic effect in
diabetic-hyperlipidaemic and normoglycemic hyperlipidaemic rats have been reported. The leaf extract also
hyperlipidaemic normoglycemic-hyperlipidaemic
exhibits antimicrobial and anti-tumourigenic properties .
tumourigenic
Phytochemical screening of the plant has revealed the presence of saponins, glycosides and tannins, which are
mical
known to be bioactive purgative principles. Flavonoids are also present in bitter leaf and three flavones – luteolin,
luteolin 7-0-beta-glucuronoside and luteolin 7-0-beta-glucoside has been identified. These flavones possess
glucuronoside glucoside
antioxidant activity and may play a beneficial role in cancer prevention, and offer some protection against
diabetes and atherosclerosis. Also, the high content of the antioxidant vitamin C present in V. amygdalina leaves
vitamin
may play a role in these18..
Medicinal Uses
Anticancer activity Vernonia amygdalina (VA) is increasingly emerging as a very strong candidate for breast
cancer treatment. VA may be used alone or in combination (adjuvant) with known drugs. Previous reports show
that low concentrations (microgram/ml) of water soluble leaf extracts of a Nigerian edible plant, V. amygdalina
water-soluble
(VA), potently retards the proliferative activities of ER+ human breast cancerous cells (MCF
(MCF-7) in vitro in a
concentration-dependent fashion. However, the anti proliferative activities of VA in either ductal or ER
dependent anti-proliferative ER-
carcinoma cells have not been characterized13.
Vernonia amygdalina extracts may help suppress, delay, or kill cancerous cell in many ways, such as:
• Induction of apoptosis as determined in cell culture and animal studies21.
• Enhanced Chemotherapy Sensitivity - V. amygdalina extracts may render cancerous cells to be more
sensitive to chemotherapy22.
• Inhibition of the growth or growth signals of cancerous cells12.
• Suppression of metastasis of cancerous cells in the body by the inhibition of NFҡB is an anti
anti-apoptotic
transcription factors as demonstrated in animal studies21.
• Reduction of estrogen level in the body by the suppression of Aromatase activity5. The Involvement of
suppression
blood estrogen level in the etiology of estrogen receptor (ER) positive breast cancer has been widely
reported6. Additional source of estrogen production in humans besides the ovary and adrenal gland is
the conversion of testosterone to estrogen in a reaction catalyzed by Aromatase. Many studies have
e
shown positive correlations between blood estrogen levels and breast cancer risks6. Therefore,
compounds that inhibit Aromatase activity are used for the treatment of breast cancer.
treatment
• anti-oxidant benefits8.
Antioxidants - V. amygdalina may provide anti
• Enhancement of the immune system - Many studies have shown that V. amygdalina extracts may
strengthen the immune system through many cytokines (including NFҡB, pro inflammatory molecule)
pro
regulation22.
Hypolipidaemic Activity
Administration of an aqueous V. amygdalina leaf extract to the hyperlipidaemic animals caused a decrease
in plasma TC, LDL-C, TAG, and VLDL and an increase in plasma HDL C concentration.
C, HDL-C
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Results obtained reveals hyperlipidaemia was successfully induced in albino New Zealand rabbits by
lts
feeding them a basal diet supplemented with a non phosphorylated egg yolk extract. Administration of an
non-phosphorylated
aqueous V. amygdalina leaf extract to the hyperlipidaemic animals caused a decrease in plasma TC, LDL
hyperlipidaemic LDL-C,
TAG, and VLDL and an increase in plasma HDL C concentration. This indicates that aqueous V. amygdalina
HDL-C
leaf extract may be useful for the control of these blood lipids in the prevention and treatment of CH 18.
CHD
Antidiabetic activity Previous study showed that the aqueous extract of V. amygdalina significantly lowered
alloxan-induced diabetic rabbits7.
blood glucose levels after oral administration to alloxan
Studies conducted using streptozotocin -induced diabetic laboratory animals showed that V. amygdalina
administration decreased blood glucose by 50% compared to untreated diabetic animals17.
Antimalarial activity Extracts from the leaves and root bark of Vernonia amygdalina are assessed for
antimalarial activity against drug-sensitive Plasmodium berghei in mice. Leaf extract produced 67% suppression
sensitive
of parasitaemia in the four-day test, while root bark extract produced 53.5% suppression. These results are
day root-bark
significant when compared to a placebo1.
Result
Evaluation of the Antioxidant Activity and Lipid Peroxidation of the Leaves of Vernonia amygdalina14
This study evaluated the in vitro antioxidant activity of water (VAWE) and ethanol (VAEE) extracts of the
leaf of Vernonia amygdalina. The antioxidant activity of each extract was evaluated using various radicals or
each
oxidation systems. The total phenolic or flavonoid contents and their correlation with total antioxidant activity
were also evaluated. VAEE exhibited antioxidant scavenging potential comparable to butylat hydroxyanisole
butylated
(BHA), but higher than butylated hydroxytoluene (BHT) or VAWE. The total antioxidant scavenging activity of
the extracts measured as the millimolar (mM) equivalent of vitamin C gave VAEE a 10.09 ± 1.63, which is
comparable to BHA with a 9.31 ± 1.17, but higher than VAWE with an 8.75 ± 1.28 or BHT with a 6.14 ± 2.01.
.31
Extracts inhibited bleaching of B carotene, oxidation of linoleic acid and lipid peroxidation induced by
B-carotene,
Fe2+/ascorbate in a rat liver microsomal preparation.
Table 1: Total phenolic and flavonoid content and total antioxidant activity of the extracts of V.
amygdalina.
Vernonia amygdalina extract Total antioxidant activity a Flavonoids b Phenolics c
Water Extract (VAWE) 8.75 ± 1.28 5 59.27 ± 3.61 271.14 ± 2.41
Ethanol Extract (VAEE) 10.09 ± 1.63 8 84.61 ± 3.58 397.48 ± 3.07
BHA 9 9.31 ± 1.17
BHT 6 6.14 ± 2.01.
Values are mean ± SD. n=4, significance is set at p≤0.001.
a: measured as mM equivalent of vitamin C.
b: measured as mg quercetin per 100 g dried plant material.
C: measured as mg gallic acid per 100 g dried plant material.
The Effect of Ethanolic Extract of Vernonia amygdalina Leaves on Some Pharmacokinetic Parameters of
Chloroquine in Rats10.
Studies were done to find out if there exist significant interactions between Chloroquine and Vernonia
amygdalina when the extract administered 1 hr before Chloroquine or simultaneously with Chloroquine to
different group of albino rats (Whister strain). Three groups of rats were used, with each group comprising of
groups
25 rats and one control group. The Chloroquine level was measured using UV spectrophotometer. The results
UV-spectrophotometer.
indicate significant interaction (p<0.5) in the group to which the extract was administered simu
simultaneously with
Chloroquine. There was a decrease in the level of drug in circulation as evidenced by lower values of AUC
(297.52±8.45 vs 333.22±24.99) and C max (74.6±1.02 vs 76.6±3.07) comparing experimental group with
C-max
control.
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Table 2: Serum concentration of Chloroquine
tion
Time points (mins) Group A (CQ only) µg/ml Group B (VA before CQ) Group A (VA + CQ)
µg/ml µg/ml
15 22.32±6.82 26.98±17.09 24.96±21.79
30 62.10±11.71 62.37±27.55 71.33±3.27
60 75.63±3.92 75.44±2.71 70.61±2.82
120 69.31±9.27 65.19±8.05 68.14±6.55
300 67.56±1.57 59.86±1.73 51.71±5.83
Mean ±SD n=5: *p<0.05:
CQ only: groups to which only Chloroquine was administered (control);
VA before CQ: groups to which chloroquine was administered 1 hr after administration of Vernonia
amygdalina;
CQ+VA: groups to which both Chloroquine and Vernonia amygdalina extract were administered together.
Table 3: Comparison of pharmacokinetic parame
parameters
Pharmacokinetic Group A (CQ only) Group B (VA before CQ) Group A (VA + CQ)
µg/ml µg/ml µg/ml
AUC (µg/h/ml) 333.22±24.99 307.50±21.44 297.52±8.45
Ka (µg/h/ml) 4.25±1.68 5.45±2.23 12.78±2.82
Ke (µg/h/ml) 0.03±0.01 0.04±0.02 0.09±0.04
Cmax (µg/h/ml) 78.60±3.07 77.40±2.24 74.60±1.02
tmax (h) 1.26±0.28 1.01±0.07 0.86±0.33
t1/2 el (h) 32.9±19.00 21.70±11.2 8.90±2.90
Mean ±SD n=5: *p<0.05:
In Vivo Antimalarial Activity of Vernonia Amygdalina1
Extracts from the leaves and root bark of Vernonia amygdalina are assessed for antimalarial activity against
drug-sensitive Plasmodium berghei in mice. A standard inoculums of 1 x 10 7 infected erythrocytes is used, and
sensitive
leaf and root-bark extracts of 500 m
bark mg/kg, 250 mg/kg or 125 mg/kg are used in a four-day suppression test and a
day
Rane test of established infection. Leaf extract produced 67% suppression of parasitaemia in the four
four-day test,
while root-bark extract produced 53.5% suppression. These results are significant when compared to a placebo
bark
Effect of aqueous extract of Vernonia amygdalina leaves on plasma
lipids of hyperlipidaemic adult male albino New Zealand rabbits18
This study investigated the effect of an aqueous extract of Vernonia amygdalina leaves on the lipid profile
leaves
of hyperlipidaemic adult male albino rabbits. Three groups of rabbits were employed in the study: a healthy
control group fed only 1ml groundnut oil/ kg body weight (A) and two groups (B and C), in which
hyperlipidaemia was induced by feeding 100mg/ ml groundnut oil/ kg body weight of a non
d non-phosphorylated egg
yolk extract for one week. For the next 14 days Group B of the hyperlipidaemic rabbits received the egg yolk
extract while Group C animals were given, in addition, 200mg/kg body weight of the aqueous extract of V.
body
amygdalina leaves twice daily. The control group (A) received only groundnut oil. All animals were fed
grower’s mash and water throughout the course of the experiment. Assay of lipids showed significant difference
(P<0.05) in total cholesterol (TC), and low density lipoprotein (LDL C) between groups A and C. Very low
<0.05) (LDL-C)
density lipoprotein (VLDL), triacylglycerol (TAG) and high density lipoprotein (HDL C) were not significantly
(HDL-C)
different between the two groups. Relative to the control, treatment with the extract decreased plasma TC and
LDL, but normalized VLDL, TAG and HDL C. Change in the lipid concentration was progressive, with TC,
HDL-C.
TAG, VLDL and LDL-C being lower and HDL C higher in the second week than in the first w
C HDL-C week after
commencement of treatment.
Vernonia amygdalina: Anticancer Activity13
Evidence suggests that most chemotherapeutic agents are less effective as treatment in patients with
estrogen receptor-negative (ER-) breast carcinomas compared to those with estrogen receptor
) receptor-positive (ER+)
breast carcinomas. Moreover, African American Women (AAW) is disproportionately diagnosed with ER ER-
breast cancer compared to their white counterparts. Novel therapies effective against ER breast carcinomas are
ER-
urgently needed to ameliorate the health disparity. Previous reports show that low concentrations (microgram/ml)
ded
of water-soluble leaf extracts of a Nigerian edible plant, V. amygdalina (VA), potently retards the proliferative
soluble
activities of ER+ human breast cancerou cells (MCF-7) in vitro in a concentration
cancerous 7) concentration-dependent fashion.
However, the anti-proliferative activities of VA in either ductal or ER carcinoma cells have not been
proliferative ER-
characterized. The exposure of BT 549 to increasing concentrations of VA (10, 100, and 10
BT-549 1000 µg/mL)
inhibited cell growth by approximately 14 % (P<0.05), 22 % (p<0.05), and 50 % (p<0.005) respectively. The
cell count studies were corroborated by DNA synthesis studies. Treatments of BT 549 with 10, 100, and 1000
BT-549
µg/mL VA inhibited DNA synthesis in a concentration dependent fashion by 22 %, 76 % (P<0.05), and 86 %
synthesis
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(p<0.01) respectively. BT-549 cells were insensitive to 10 and 100 nM paclitaxel (TAX) treatments. Isolation
549
of DNA from dried VA leaves yielded approximately 12.2 and 1 kbp genomic DNA that were Eco RI
DNA RI-insensitive
but Hind III and Bam HI-sensitive. These pieces of information may be used to enhance the safety of medicinal
sensitive.
botanical VA through authentication, and adulteration detection.
Evaluation of the anti-inflammatory activity of e
inflammatory extract of Vernonia Amygdalina9
Inflammatory response was induced by topical application of croton oil dissolved in suitable vehicle on the
rat ear. After 6 hrs, cutting out the ear quantified the response. The cut ear was weighed and the increase in
weight relative to control group was evaluated. When co applied with croton oil to the rat ear extract of V.A.
co-applied
produced a reduction in the inflammatory response when croton oil alone was applied to the rat ear. The extract
produced (69.1±2.0)% reduction of the inflammatory response produced by croton oil alone, lower than the
inflammatory
reduction of the inflammatory response produced by acetyl salicylic acid [(71.1±2.0)%].
Bioactive sesquiterpene lactones from the leaves of Vernonia amygdalina8
Phytochemical analysis of the leaves of Vernonia amygdalina yielded two known sesquiterpene lactones:
e
vernolide and vernodalol. The two compounds were tested by agar dilution method against 10 bacteria strains
and 5 fungi species. Both compounds exhibited a significant bactericidal activity against five Gram positive
activity
bacteria while lacking efficacy against the Gram negative strains. In the antifungal test, while vernolides
exhibited high activity with LC (50) values of 0.2, 0.3 and 0.4 mg/ml against Penicillium notatum, Aspergillus
flavus, Aspergillus niger and Mucor hiemalis, respectively, vernodalol showed moderate inhibitions against
avus,
Aspergillus flavus, Penicillium notatum and Aspergillus niger with LC(50) values of 0.3, 0.4 and 0.5 mg/ml,
respectively. Both compounds were ineffective against Fusarium oxysporum, a microbe known to be highly
ineffective
resistant to chemical agents. However, the antimicrobial results of this study correspond positively with the
claimed ethnomedical uses of the leaves of Vernonia amygdalina in the treatment of various infectious diseases.
various
Phytochemical investigation on the leaves of Vernonia amygdalina19
A number of known sesquiterpene lactones have been isolated from Vernonia species. Some sesquiterpene
lactones and flavonoids have isolated from Vernonia amygdalina are named below as luteolin, luteolin luteolin-
7-O-glucoside by the partial characterization of Vernonia amygdalina methanolic extract using IR and UV
glucoside
spectrum.
Hepatoprotective and antioxidant activities of Vernonia amygdalina on acetaminophen
acetaminophen-induced hepatic
damage in mice11
This study evaluated the hepatoprotective and antioxidant effects of an aqueous extract of V. amygdalina
leaves against acetaminophen-induced hepatotoxicity and oxidative stress in mice in vivo. Activities of liver
induced
marker enzymes in serum (glutamate
(glutamate-oxaloacetate transaminase, glutamate-pyruvate transaminase, lactate
pyruvate
dehydrogenase, and alkaline phosphatase) and bilirubin levels were determined colorimetrically, while catalase
activity, lipid peroxidation products, thiobarbituric aci reactive substances (TBARS), iron, and total protein
acid-reactive
concentrations were measured in liver homogenate. Acetaminophen challenge (300 mg/kg, i.p) for 7 days caused
significant (P < .01) increases in the levels of bilirubin, liver enzymes, TBARS, and iron, while catalase activity
and total protein level were reduced significantly (P < .01). Pre administration of V. amygdalina resulted in a
Pre-administration
dose-dependent (50-100 mg/kg) reversal of acetaminophen induced alterations of all the liver function
100 acetaminophen-induced
parameters by 51.9-84.9%. Suppression of acetaminophen induced lipid peroxidation and oxidative stress by the
84.9%. acetaminophen-induced
extract was also dose-dependent (50 100 mg/kg). The results of this study suggest that V. amygdalina elicits
dependent (50-100
hepatoprotectivity through antioxidant activity on acet
acetaminophen-induced hepatic damage in mice.
induced
Hypoglycemic activity of Vernonia amygdalina2.
Study conducted showed that the aqueous extract of Vernonia amygdalina significantly lowered blood glucose
levels after oral administration to alloxanalloxan-induced diabetic rabbits. Also, studies conducted using
streptozotocin-induced diabetic laboratory animals showed that V. amygdalina administration decreased blood
induced
glucose by 50% compared to untreated diabetic animals.
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Phytochemical screening of aqueous extract of Vernonia amygdalina4
Table 4: Phytoconstitution of Vernonia amygdalina aqueous extract.
Phytoconstituent Qualitative abundance
Anthraquinones +++
Soluble tannins ++
Condensed tannins +
Flavonoids +
Alkaloids +++
Indole alkaloids +++
Steroidal alkaloids +
Saponins +++
glycosides ++
Cyanogenic glycosides +
Terpenoids +++
Levels of phytoconstituents were qualitatively determined based on chemical group reactions and thin
thin-layer
chromatography on the following scale: , absent( present at low levels(+), present at moderate levels(++),
absent(-)
present at high levels(+++).
Discussion
Phytochemicals differ from phytonutrients in that they are not a necessity for normal metabolism and absence
will not result in deficiency disease. Phytochemicals are not required for the functioning of the body, but they
for
are of benefit on health and play an active role in the treatment of diseases. The presence of phytochemicals such
as sesquiterpene lactones and flavonoids namely vernolide and vernodalol, luteolin and luteolin 7-O-glucoside
luteolin-
isolated from Vernonia amygdalina has been revealed using various solvent system for extraction and analytic
techniques such as chromatography, IR and NMR for partial characterization.
Phytochemical screening of various extract of Vernonia amygdalina has shown the presence of
Anthraquinones (+++), Soluble tannins (++), Condensed tannins (+), Flavonoids (+), Alkaloids (+++), Indole
alkaloids (+), Steroidal alkaloids (+++), Saponins (+++), Glycosides (++), Cyanogenic glycosides ( (-),
Terpenoids (+++). Studies conducted to determine or/and ascertain the pharmacologic activity of Vernonia
+).
amygdalina extract has revealed that these aforementioned phytochemicals are responsible for the majority of
these observed pharmacologic activities.
Antioxidant Activity Of Vernonia amygdalina
The total flavonoids and phenolic contents correlated positively with total antioxidant activity. The total
flavonoid content also correlated well with total phenolic content. Extracts of V. amygdalina contain natural
antioxidants; have the potential to act as antioxidants against aqueous radicals and reactive species ions.
Phytochemical screening of the leaves of V. amygdalina showed the presence of saponins, tannins,
anthracene glycoside, and flavonoids. In recent years, there has been increased interest in V. amygdalina due to
ids.
its biological activities, especially regarding its antioxidant, anticancer, antiviral, and anti-inflammatory activities.
anti-
The biological activity may be due to its flavonoid content, a natural polyphenolic antioxidant that occurs in
content,
fruits and vegetables and exhibits a number of biological activities .Thus, interest in natural antioxidants has
been on the increase with much attention on plant flavonoids as possible therapeutic agent against free radical
agent
mediated diseases.
The Effect of Ethanolic Extract of Vernonia amygdalina Leaves on Some Pharmacokinetic Parameters of
Chloroquine in Rats.
There was increase rate of elimination of drugs between the experimental group and control group Ke
(.088±0.035 vs 0.027±0.017). There was no significant pharmacokinetic interaction (p>0.5) when the extract was
administered 1 hr before Chloroquine.
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The result indicates that concomitant administration of Chloroquine with Vernonia amygdalina results in
significant interaction (p<0.5) and should be avoided to maintain good plasma level of the drug and also to
prevent development of Plasmodium resistance secondary to subclinical concentration of the drug in blood.
In Vivo Antimalarial Activity Of Ve Vernonia amygdalina
The study show that in vivo administration of an ethanol extract of V. amygdalina is capable of suppressing
parasitaemia, especially during early infection; the antimalarial action being attributed to sesquiterpene lactones.
However, under the experimental conditions employed, the extracts failed to eliminate P. berghei parasites
r
completely.
Effect of Aqueous Extract of Vernonia amygdalina Leaves on Plasma
Lipids of Hyperlipidaemic Adult Male Albino New Zealand Rabbits
Relative to the control, treatment with the extract decreased plasma TC and LDL, but normalized VLDL,
ntrol,
TAG and HDL-C. Change in the lipid concentration was progressive, with TC, TAG, VLDL and LDL being
C. LDL-C
lower and HDL-C higher in the second week than in the first week after commencement of treatment.
C
Results obtained reveals hyperlipidaemia was successfully induced in albino New Zealand rabbits by
feeding them a basal diet supplemented with a non phosphorylated egg yolk extract. Administration of an
non-phosphorylated
aqueous V. amygdalina leaf extract to the hyperlipidaemic animals caused a decrease in plasma TC, LDL
af LDL-C,
TAG, and VLDL and an increase in plasma HDL concentration.
HDL-C
The results of this study clearly indicate that the administration of aqueous extract of V. amygdalina leaves
produced a hypolipidaemic effect in hyperlipidaemic rabbits, even when they were still being fed the atherogenic
uced
egg yolk extract. There are many bioactive constituents present in the extract and hence, at present, it is not
certain which of them is/are responsible for the observed effect. However, some reports have shown that
responsible
flavonoids, tannins and saponins may play some roles in antioxidant and hypolipidemic effects. As has already
been mentioned, elevated plasma LDL cholesterol concentrations are associated with increased risk of CHD.
However, the atherogenicity of the LDL particle increases after oxidative modification of its
polyunsaturated fatty acids15. The antioxidants present in the leaves may help to prevent LDL oxidation resulting
in a decrease in its atherogenicity and a reduction of CHD risk.
Anticancer Activity of Vernonia amygdalina
The exposure of BT-549 to increasing concentrations of VA (10, 100, and 1000 µg/mL) inhibited cell growth
549
by approximately 14 % (P<0.05), 22 % (p<0.05), and 50 % (p<0.005) respectively. The cell count studies were
corroborated by DNA synthesis studies. Treatments of BT 549 with 10, 100, and 1000 µg/mL VA inhibited
BT-549
DNA synthesis in a concentration dependent fashion by 22 %, 76 % (P<0.05), and 86 % (p<0.01) respec respectively.
BT-549 cells were insensitive to 10 and 100 nM paclitaxel (TAX) treatments. Isolation of DNA from dried VA
549
leaves yielded approximately 12.2 and 1 kbp genomic DNA that were Eco RI insensitive but Hind III and Bam
RI-insensitive
HI-sensitive. These pieces of information may be used to enhance the safety of medicinal botanical VA through
rmation
authentication, and adulteration detection.
Evaluation of the anti-inflammatory activity of extract of Vernonia Amygdalina
inflammatory
Findings suggest that extract of V.A. exhibits anti
anti-inflammatory activity and may explain the usefulness of the
ammatory
leaves of this plant in the treatment of inflammatory disease conditions by traditional healers.
Conclusion
The review of phytochemical screening reveals presence of bioactive phytoconstituents in methanol, ethanol,
n-hexane and aqueous extracts of Vernonia amygdalina. This study review has revealed that the observed
.
pharmacologic activities of Vernonia amygdalina is due to the singular or combined action of one or more of
these phytoconstituents detectected during phytochemical screening of various extract of Vernonia amygdalina
tectected
has shown the presence of Anthraquinones, Soluble tannins, Condensed tannins, Flavonoids, Alkaloids, Indole
alkaloids, Steroidal alkaloids, Saponins, Glycosides, Cyanogenic glycosides, Terpenoids. Vernolide and
vernodalol, luteolin and luteolin- 7 glucoside are sesquisterpene lactones and flavonoids responsible for
7-O-glucoside
majority of observed pharmacologic activities amongst which are but not limited to antidiabetic activity,
anti-inflammatory activity, antiparasitic activity, antioxidant and lipid peroxidation activity, hepatoprotective
inflammatory
activity, anticancer activity.
References
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