This document discusses clinical sample collection and preservation for virus detection. There are two types of virus samples: fluid samples like blood, serum, and urine, and solid samples like organs. When transporting specimens to the lab, they must be labeled and stored in viral transport media with ice or cold packs. Common specimens include blood, urine, stool, saliva, semen, eye secretions, CSF, cervical samples, and throat/nasal swabs. Viruses can be preserved long-term by freeze drying, storage in liquid nitrogen, or as purified nucleic acids at cold temperatures.
Serological test for virus identificationPlock Ghosh
This presentation consist of detailed study of serological method of virus identification. Basically ELISA is vastly used for virus detection. Western blot method is used for HIV identification.
Serological test for virus identificationPlock Ghosh
This presentation consist of detailed study of serological method of virus identification. Basically ELISA is vastly used for virus detection. Western blot method is used for HIV identification.
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Qalification
AHLAD T O
MSc MLT (Biochemistry)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Proteus microbiology
#Medical
#Microbiology
#Biochemistry
#Mallu Medicos Lounge
##MalluMedicosLounge
#MLT
#Channel introduction
#HealthAndVoyage
#New Youtube Channel introduction
#Gram-negative
#Enterobactericea
#Weil Felix Test
#PROTEUS - causes, symptoms, diagnosis, treatment, pathology
Introduction, classification of virus, collection, Transport, & Storage of sample for Viral diagnosis. Staining Techniques used in virology,
Processing of sample for viral diagnosis (Egg Inoculation & Tissue culture)
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..
Qalification
AHLAD T O
MSc MLT (Biochemistry)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Proteus microbiology
#Medical
#Microbiology
#Biochemistry
#Mallu Medicos Lounge
##MalluMedicosLounge
#MLT
#Channel introduction
#HealthAndVoyage
#New Youtube Channel introduction
#Gram-negative
#Enterobactericea
#Weil Felix Test
#PROTEUS - causes, symptoms, diagnosis, treatment, pathology
Taklimat berkenaan MERS-COV (Middle East Respiratory Syndrome-Corona Virus) berkenaan pengurusan sample yang diambil dari pesakit.
Virus ini mula tersebar di Arab Saudi, dan perhatian lebih perlu diberikan kepada jemaah-jemaah yang baru pulang dari Umrah di Makkah & Madinah, Arab Saudi.
An approache for different kinds of sampling for diagnosis of animal diseases
( Scientific activity done at Al Ain , UAE . Under the supervesion of Department of Agriculture and livestock
Similar to collection and preservation of virus.pptx (20)
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
1. Virology 3th class
lab 2
Clinical Samples Collection & Preservation
By :
Assist. lecturer .Mawahb Hatem Mones
2. virus pathogenic sample.
There are two types of virus samples:-
1-Fulid sample(Blood, s.f ,serum , c.s.f, urine ).
2- Solid sample (Lung, heart ,Liver , Kidney ,spleen , stool, )
3. Specimens sending to the viruses Laboratory.
1- Anticoagulated blood.
2- Urine Specimens
3-Skin scraping :in case infections caused by poxvirus and herpes virus.
4-Faeces: In case enterovirus infection caused by Rota virus.
5- Swabs: Collected a specimen from (nose, eye, trachea, vaginal, Rectal).
6- Organs (heart, spleen, liver, kidney, lung. In case of died animals it’s called
(Autopsy).
7- Biopsy Specimen sending or part from infected organ to human or animal, when are
living.
4. Transporting of specimens to the viruses Laboratory.
Must be the following information when transport the pathogenic specimens to the
Labor.
1- Specimens transport into strong a sterile screw cap tube or bottles which contains
viral transport medium like 199 media add to mixture antibiotics to preserve the
Specimens.
2- They should be transported insulated box that contains ice packs or ice cubes in
plastic bags.
3- Using some time preservated substance like glycerin 50% or phosphate Buffer saline
5. Clinical Samples Collection
1 - Blood Specimens :Blood Include Serum or plasma sample could be obtained from
venous blood, which can be performed by the laboratory personnel. For serum or
plasma sample, first 2-3 ml of venous blood is collected using sterile syringe and
needle from a patient putting into a clean, dry, sterile tube. Care must be taken to
avoid hemolysis, since this may produce a false-positive result .
A.Serum: is required, allow the whole blood to clot at room temperature for at
least one hour and centrifuge the clotted blood for 10 minutes at 2000 rpm. Then
transfer the serum to a labeled tube.
6. B.Plasma: sample is obtained by treating fresh blood with an anticoagulant,
centrifuge and separate the supernatant. The specimen should be free from
hemolyzed blood. Finally, the tube should be labeled with full patient's
identification (Age, Sex, code no, etc.). The test should be performed within
hours after sample collection, if testing cannot be performed immediately, serum
may be stored between 2°C and 8°C for up to 72 hours. If this could not be done
preserve it at -200°c
7. Blood separation
Plasma is that part of the blood, which contains blood clotting agent
called as fibrinogen.
serum is the fluid part of the blood and does not contain clotting agent.
8. 2- Urine Specimens: The specimen should be collected at the onset of illness
if congenital disease is suspected. - Examining 2 - 3 consecutive urine
specimens. - (10 - 20 ml of urine) are collected in sterile containers and
transported to the laboratory on wet ice or cold pack. , examples; polyoma
virus
3-Stool Specimens: Stool specimens for viral isolation attempts should be
collected as soon as possible, and usually no later than 7-10 days after onset
of illness. Enteroviruses may be excreted for weeks so if infection with these
viruses is suspected, stool specimens collected later than 10 days post onset
may be collected .example ; Rota virus .
4- Saliva: collected by aspiration or expectoration into a sterile container also
may be used for virus isolation. Ex ; corona virus
5-Semen Specimens: Semen specimens collected into sterile screw-cap jars
should be sent to the laboratory on wet ice or cold pack
9. 6-Eye Specimens: A swab moistened in sterile saline is used to collect
secretions from the conjunctiva.
7- Cerebrospinal Fluids (CSF): For virus isolation 3-4 ml of CSF should be
collected no later than 7-10 days after onset of illness
10. 8-Cervical Specimens: The specimen is transported to the laboratory on wet ice or a cold
pack. If it cannot be tested within 48 hours it should be frozen below - 70 C, Its can make it as
a paraffin -wax block or pap smear for cytology and virus detection.
9-Vesicular Lesion Specimens: Vesicular fluids and cellular material from the base of lesions
should be collected for virus isolation during the first 3 days of the eruption. Vesicles are
washed gently with sterile saline and the vesicular fluids are aspirated with a 26-gauge or 27-
gauge needle attached to a tuberculin syringe, or with a capillary pipette.
11. 12- Throat and Nasopharyngeal Specimens: Virus isolation is most successful if
respiratory specimens are taken within the first 3 days of illness, and they should be
collected no later than 5 days after onset. For virus isolation, swabs from both the
throat and nasal passage should be collected. Note, respiratory specimens should not be
frozen at -20 C temperatures as this will markedly reduce chances of isolating
respiratory viruses. In genera freezing should be avoided if possible
Example : Influenza viruses, Adeno virus, Corona virus and others.
12. Viruses preservation
In general, DNA viruses are more stable than RNA viruses but both types are extremely stable and can be
preserved relatively easily.
Many viruses can be kept for months at refrigerator temperatures and stored for years at very low
temperatures without the need for special preservatives or carefully regulated slow freezing techniques.
Their simple structure, small size and the absence of free water are largely responsible for this stability.
Viruses with lipid envelopes are often less stable than non-enveloped viruses at ambient temperatures but
survive well at ultra-low temperatures or in the freeze-dried state
13. Methods for long-term virus preservation
1. Freeze dried preparations of virus can be maintained at 4°C in the dark and lower
temperatures increase the storage time.
2. Virus is retained for very long periods in liquid nitrogen. However, most viruses will survive
almost in liquid nitrogen.
3. Proteins are effective protectants for virus cryopreservation. The suspending medium of choice
for many viruses is tissue culture medium containing added serum or other proteins, at
concentrations up to or greater than 10%
14. the proteins possible provide buffering capacity against pH changes, assist in colloidal
dispersion of the virus particles and reduce or inhibit other processes that damage
nucleic acids. Viruses contained in serum or tissues from human or animal specimens
can be stored at ultra low temperatures without further treatment.
4. High titer virus preparations are retain for long-term storage longer than a low titer
preparation.
5. Freeze drying ( lyphoilization ) viruses for long term preservation .This is probably
the most satisfactory method of preserving viruses for very long periods
15. 6-It is good method to preserve small volumes of virus suspension. In general, virus
infectivity is maintained more effectively when samples are preserved in small volumes
because rapid freezing and thawing of a virus preparation is less harmful to the virus than
slow freezing and thawing.
7-Viruses can be preserved for long periods as nucleic acid. The purified nucleic acid viral
RNA and many DNA viruses This principle can be utilized to preserve these viruses for
very long periods of time. The ethanol precipitated RNA and DNA can be stored almost
indefinitely at 4°C (or lower temperatures) under ethanol. The ethanol is important for long
term storage of RNA, to inhibit enzymes that breakdown RNA.
Editor's Notes
من المحتمل أن توفر البروتينات قدرة تخزين مؤقت ضد تغيرات الأس الهيدروجيني، وتساعد في التشتت الغروي لجزيئات الفيروس وتقلل أو تمنع العمليات الأخرى التي تلحق الضرر بالأحماض النووية. يمكن تخزين الفيروسات الموجودة في مصل أو أنسجة العينات البشرية أو الحيوانية في درجات حرارة منخفضة للغاية دون مزيد من العلاج.
7- يمكن حفظ الفيروسات لفترات طويلة كحمض نووي. يعد الحمض النووي المنقى لفيروسات الحمض النووي الريبي الموجب (أي تلك التي يكون فيها الحمض النووي الريبي الفيروسي هو الحمض النووي الريبي المرسال) والعديد من فيروسات الحمض النووي (تلك التي لا تحتوي على إنزيمات أساسية في بنيتها) معدية. ويمكن الاستفادة من هذا المبدأ للحفاظ على هذه الفيروسات لفترات طويلة جدًا من الزمن. يمكن تخزين الحمض النووي الريبي (RNA) والحمض النووي (DNA) المترسب بالإيثانول إلى أجل غير مسمى تقريبًا عند 4 درجات مئوية (أو درجات حرارة أقل) تحت الإيثانول. الإيثانول مهم لتخزين الحمض النووي الريبي (RNA) على المدى الطويل، لمنع الإنزيمات التي تحطم الحمض النووي الريبي (RNA).