Centrioles duplicate in a defined cycle. A parental pair of centrioles lose their orthogonal arrangement in G1 and form single centrioles. Near S phase, each parental centriole nucleates a procentriole that elongates into a daughter centriole by late G2 or early G1. During mitosis, the centriole pairs migrate to opposite sides of the cell, forming the mitotic spindle. Spindle fibers form from microtubules that elongate and attach to kinetochores as centrioles move to cell poles, enabling chromosome movement during cell division.