The document summarizes research isolating and characterizing stem cells from human fetal liver. Key steps included isolating cells using collagenase digestion and magnetic sorting for CD34+ markers. Cell viability was assessed using trypan blue exclusion. Immunocytochemistry and PCR were used to identify expression of markers AFP, ALB and CK18. MTT assays were conducted to assess cell proliferation and activity in response to potential toxic materials. Results showed 49.23% viability in isolated cells and expression of stem cell markers, indicating successful isolation of human fetal liver stem cells. Further characterization of gene expression profiles was recommended.