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Cell Structure
& Function
Cell Theory
• All living things are made up of cells.Cells
are building blocks of our body (as bricks
are building blocks of a house).
• Cells are the smallest working units of all
living things.
• All cells come from pre-existing cells
through cell division.
• All living things have 3 common features:
Reproduction,Locomotion n Digestion
Definition of Cell
 A Cell is the structural and
functional unit of all living
organism.
 The cell is the smallest unit that is
capable of performing life
functions as
reproduction,locomotion and
digestion.
DISCOVARY
• By Robert Hook in 1665
• He observed boxes in bark of tree(cork)
under microscope.He named these boxes
Cells
• After staining with Eosine/Hematoxyline
cytoplasm looks pink and nucleus blue.
Examples of Cells
Amoeba Proteus
Plant Stem
Red Blood Cell
Nerve Cell
Bacteria
CELLS VARY IN
• No. of cells;
• Unicellular(Amoeba,bacteria)
• Multicellular(Animals-trillions-elepphant)
• Size of cells;
• 0.1-0.5µm(bacteria) to170x130mm(ostrich)
• Shape of cells;
• Round(RBC’s),spherical(WBC,s),elongate
d(Epithelial),Spindle(muscle),branched(ner
ve)
Two Types of Cells
•Prokaryotic
•Eukaryotic
1
2
Prokaryotes
Cell membrane
Cytoplasm
Types of Cells
• Eukaryots;Cells having true
nucleus(Eu=true,karyo=nucleus
• Prokaryots;comprise bacteria which are
considered by scientists to have evolved
before eukaryotes
(pro=before,karyo=nucleus)
Prokaryotic-Bacteria
• Oldest form of life on earth
• Outermost capsule-cell wall-cell memb.
• Cytoplasm contain free single circular
DNA (nucleoid/plasmid) without
welldefined nucleous for reproduction.
• Ribosomes consist of rRNA n proteins.
• External structures-flagella,pili etc
Prokaryotic Cells
are the most complex form of life.
They have nucleus
Contain organelles surrounded by membranes
They are multicellular organisms (composed of
many cells).
Most living organisms
Examples: animals and plants.
Eukaryotic
Eukaryotic
Plant Animal
Common Characteristics
• All cells are surrounded by a barrier called
the cell membrane
• They contain the molecule that carries
biological information-DNA
Isolation of cell components
• The cellular compt. are obtained by
differential centrifugation of cells,after they
have been gently broken by one of the
following methods;
• Grinding,In Hypotonic soln.,Alternative
freezing thawing,Ultrasonification,Enzymic
treatment.
• The homogenized cells can be
fractionated by differential centrifugation at
various forces of gravity(1000-150,000g
for 10min-3hrs).
Differential Centrifugation
Eukaryotic cell
Cell-Tissue-Organ-System
Cell-Tissue-Organ-System-Organism
Cell Parts
Cell membrane
Cytoplasm- Organeles
Nucleous
CELL STRUCTURE AND FUNCTION
“Typical” Animal Cell
Cell Structures
• The Plasma Membrane—A Cell's Protective Coat
• The Cytoskeleton—A Cell's Scaffold
• The Cytoplasm—A Cell's Inner Space
• Genetic Material
Cell Organelles include
• The Nucleus—A Cell's Center
• The Endoplasmic Reticulum and the Golgi Apparatus
Macromolecule Manager
• The Ribosome—The Protein Production Machine
• Mitochondria and Chloroplasts—The Power Generators
• Lysosomes and Peroxisomes—The Cellular Digestive
System
Cytoplasm and Organelles
• Cytoplasm lies between the cell
membrane and nucleus and contains the
organelles.
• Cytosol is a gelatin like aqueous fluid that
contains salts, minerals and organic
molecules
ORGANELLES
Nucleus
Nucleus
• Most prominent structure
• Maintains its shape with a protein skeleton called the
nuclear matrix
• Double membrane – nuclear envelope
• Inside the envelope – chromatin (DNA & protein)
• When the cell is about to divide it forms the
chromosomes-made of DNA.
• Stores heriditary information in its DNA-transfer by
replication.
• RNA is copied from DNA-transcription- forming mRNA
• m-RNA travels from nucleus to the cytosol through small
holes in the envelope – nuclear pores
• Contains the nucleolus – the site where ribosomes are
synthesized
Nucleolus
• Inside nucleus
• Contains RNA to build
proteins
• Site where ribosome
are synthesized
Endoplasmic Reticulum
Endoplasmic Reticulum
• System of membranous tubules and sacs
• Intracellular Highway – molecules move from one
part of the cell to another
• 2 types – rough and smooth
• Rough ER-studded with ribosomes –synthesis of
proteins with posttranslational modifications- to be
used by the cell organelles, membrane or secreted
from the cell
• Smooth ER - no ribosomes-has enzymes for:
Synthesis of TAG & Phospholipids in enterocytes
Synthesis of steroids in gland cells
Has cyto.P450 for detoxification in liver
Regulation of calcium in muscle cells
Ribosomes
Ribosomes
• Most numerous
• Not surrounded by a membrane
• Synthesized in nucleolus in the nucleous.
• Made up of proteins and RNA
• RNA is packaged into the ribosomes then transported to
the cytosol
• Some ribosomes are free
• Some are attached to the endoplasmic reticulum
• Protein is synthesized in the ribosomes
• Proteins to be used in the cytosol – free ribosomes
• Proteins to be exported or inserted in the cell membrane
– ribosomes on the ER
Golgi Apparatus
• System of membranes
• Series of flattened sacs
with convex shape
• Works with the ER to
modify proteins
Processing, packaging
distributing proteins to
lysosomes,secretory
vesicles and pl.memb.
CYTOSKELETON
• .
CYTOSKELETON
• Network of long flexible fibrous protein strands,maintains
the shape and size of cell
• Microtubules – largest strands are hollow cyllindrical
tubes begin in nu n extend to pl memb. made up of
tubulin protein
– Help the cell divide by forming spindle
- Responsible for positioning of organelles
- Participates in the movement of vesicles
• Microfilaments – threads made of actin (protein) –
smallest strands-control the shape of cell n movement of
cell surface to move, divide n engulf.
• Intermediate filaments- are composed of fibrous
protein polymers-provide structural support to memb n
other cellular components.
Mitochondria
They are called the
“POWER HOUSE”
of the cell
Mitochondria
• Sites of chemical reactions that transfer energy from organic
compounds to ATP
• ATP- main energy source for cells
• Size 1-2 µ,1-1000 in number
• Cells with high energy requirements have more mito.
e.g.CNS,muscle,liver
• Have 2 membranes
• Smooth outer membrane serves as a boundary between the
mitochondria and the cytosol
• Inner membrane has many folds called Cristae – they enlarge the
surface area for more chemical reactions
• Have their own DNA n RNA(maternal)
• Mito has 120 proteins(13 encoded by mito.DNA. Mito can replicate.
• Mutations cause muscle n nerve,renal diseases e.g. LHON &
MERRF
LYSOSOMES
LYSOSOMES
• Small spherical organelles of digestion.
• Enclose hydrolytic enzymes, within single
membranes with ATPase to keep ph 5.5
• Digest proteins, carbohydrates, lipids, DNA and
RNA(Endocytosis)
• Eliminating unwanted material- old
organelles,misfold proteins
bacterias,viruses,tissue remodeling after injury
• Destroyed compt. are recycled
• L.Enz Defficiency leads to storage dis, L-cell
disease(T.sachs,Gaucher,Pompaei diseases)
PEROXYSOMES
• Spherical with single emb.,similar to
lysosomes in size,involved in oxidative
reactions using molecular O2,producing
H2O2 degraded by catalase.
• Function in oxidation of long chain FA &
synth of bile acids from cholestrol
• Can replicate by division like mito.
Label the parts of the cell
http://www.phschool.com/atschool/phbio/activities/cbd-3072/simbase.htm
http://www.harcourtschool.com/activity/cell/cell.htm

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Cell organele AIMC

  • 2. Cell Theory • All living things are made up of cells.Cells are building blocks of our body (as bricks are building blocks of a house). • Cells are the smallest working units of all living things. • All cells come from pre-existing cells through cell division. • All living things have 3 common features: Reproduction,Locomotion n Digestion
  • 3. Definition of Cell  A Cell is the structural and functional unit of all living organism.  The cell is the smallest unit that is capable of performing life functions as reproduction,locomotion and digestion.
  • 4. DISCOVARY • By Robert Hook in 1665 • He observed boxes in bark of tree(cork) under microscope.He named these boxes Cells • After staining with Eosine/Hematoxyline cytoplasm looks pink and nucleus blue.
  • 5. Examples of Cells Amoeba Proteus Plant Stem Red Blood Cell Nerve Cell Bacteria
  • 6. CELLS VARY IN • No. of cells; • Unicellular(Amoeba,bacteria) • Multicellular(Animals-trillions-elepphant) • Size of cells; • 0.1-0.5µm(bacteria) to170x130mm(ostrich) • Shape of cells; • Round(RBC’s),spherical(WBC,s),elongate d(Epithelial),Spindle(muscle),branched(ner ve)
  • 7. Two Types of Cells •Prokaryotic •Eukaryotic
  • 9. Types of Cells • Eukaryots;Cells having true nucleus(Eu=true,karyo=nucleus • Prokaryots;comprise bacteria which are considered by scientists to have evolved before eukaryotes (pro=before,karyo=nucleus)
  • 10. Prokaryotic-Bacteria • Oldest form of life on earth • Outermost capsule-cell wall-cell memb. • Cytoplasm contain free single circular DNA (nucleoid/plasmid) without welldefined nucleous for reproduction. • Ribosomes consist of rRNA n proteins. • External structures-flagella,pili etc
  • 12. are the most complex form of life. They have nucleus Contain organelles surrounded by membranes They are multicellular organisms (composed of many cells). Most living organisms Examples: animals and plants. Eukaryotic
  • 14. Common Characteristics • All cells are surrounded by a barrier called the cell membrane • They contain the molecule that carries biological information-DNA
  • 15. Isolation of cell components • The cellular compt. are obtained by differential centrifugation of cells,after they have been gently broken by one of the following methods; • Grinding,In Hypotonic soln.,Alternative freezing thawing,Ultrasonification,Enzymic treatment. • The homogenized cells can be fractionated by differential centrifugation at various forces of gravity(1000-150,000g for 10min-3hrs).
  • 20. Cell Parts Cell membrane Cytoplasm- Organeles Nucleous CELL STRUCTURE AND FUNCTION
  • 22. Cell Structures • The Plasma Membrane—A Cell's Protective Coat • The Cytoskeleton—A Cell's Scaffold • The Cytoplasm—A Cell's Inner Space • Genetic Material Cell Organelles include • The Nucleus—A Cell's Center • The Endoplasmic Reticulum and the Golgi Apparatus Macromolecule Manager • The Ribosome—The Protein Production Machine • Mitochondria and Chloroplasts—The Power Generators • Lysosomes and Peroxisomes—The Cellular Digestive System
  • 23. Cytoplasm and Organelles • Cytoplasm lies between the cell membrane and nucleus and contains the organelles. • Cytosol is a gelatin like aqueous fluid that contains salts, minerals and organic molecules
  • 26. Nucleus • Most prominent structure • Maintains its shape with a protein skeleton called the nuclear matrix • Double membrane – nuclear envelope • Inside the envelope – chromatin (DNA & protein) • When the cell is about to divide it forms the chromosomes-made of DNA. • Stores heriditary information in its DNA-transfer by replication. • RNA is copied from DNA-transcription- forming mRNA • m-RNA travels from nucleus to the cytosol through small holes in the envelope – nuclear pores • Contains the nucleolus – the site where ribosomes are synthesized
  • 27. Nucleolus • Inside nucleus • Contains RNA to build proteins • Site where ribosome are synthesized
  • 29. Endoplasmic Reticulum • System of membranous tubules and sacs • Intracellular Highway – molecules move from one part of the cell to another • 2 types – rough and smooth • Rough ER-studded with ribosomes –synthesis of proteins with posttranslational modifications- to be used by the cell organelles, membrane or secreted from the cell • Smooth ER - no ribosomes-has enzymes for: Synthesis of TAG & Phospholipids in enterocytes Synthesis of steroids in gland cells Has cyto.P450 for detoxification in liver Regulation of calcium in muscle cells
  • 31. Ribosomes • Most numerous • Not surrounded by a membrane • Synthesized in nucleolus in the nucleous. • Made up of proteins and RNA • RNA is packaged into the ribosomes then transported to the cytosol • Some ribosomes are free • Some are attached to the endoplasmic reticulum • Protein is synthesized in the ribosomes • Proteins to be used in the cytosol – free ribosomes • Proteins to be exported or inserted in the cell membrane – ribosomes on the ER
  • 32. Golgi Apparatus • System of membranes • Series of flattened sacs with convex shape • Works with the ER to modify proteins Processing, packaging distributing proteins to lysosomes,secretory vesicles and pl.memb.
  • 34. CYTOSKELETON • Network of long flexible fibrous protein strands,maintains the shape and size of cell • Microtubules – largest strands are hollow cyllindrical tubes begin in nu n extend to pl memb. made up of tubulin protein – Help the cell divide by forming spindle - Responsible for positioning of organelles - Participates in the movement of vesicles • Microfilaments – threads made of actin (protein) – smallest strands-control the shape of cell n movement of cell surface to move, divide n engulf. • Intermediate filaments- are composed of fibrous protein polymers-provide structural support to memb n other cellular components.
  • 35. Mitochondria They are called the “POWER HOUSE” of the cell
  • 36. Mitochondria • Sites of chemical reactions that transfer energy from organic compounds to ATP • ATP- main energy source for cells • Size 1-2 µ,1-1000 in number • Cells with high energy requirements have more mito. e.g.CNS,muscle,liver • Have 2 membranes • Smooth outer membrane serves as a boundary between the mitochondria and the cytosol • Inner membrane has many folds called Cristae – they enlarge the surface area for more chemical reactions • Have their own DNA n RNA(maternal) • Mito has 120 proteins(13 encoded by mito.DNA. Mito can replicate. • Mutations cause muscle n nerve,renal diseases e.g. LHON & MERRF
  • 38. LYSOSOMES • Small spherical organelles of digestion. • Enclose hydrolytic enzymes, within single membranes with ATPase to keep ph 5.5 • Digest proteins, carbohydrates, lipids, DNA and RNA(Endocytosis) • Eliminating unwanted material- old organelles,misfold proteins bacterias,viruses,tissue remodeling after injury • Destroyed compt. are recycled • L.Enz Defficiency leads to storage dis, L-cell disease(T.sachs,Gaucher,Pompaei diseases)
  • 39.
  • 40. PEROXYSOMES • Spherical with single emb.,similar to lysosomes in size,involved in oxidative reactions using molecular O2,producing H2O2 degraded by catalase. • Function in oxidation of long chain FA & synth of bile acids from cholestrol • Can replicate by division like mito.
  • 41. Label the parts of the cell http://www.phschool.com/atschool/phbio/activities/cbd-3072/simbase.htm http://www.harcourtschool.com/activity/cell/cell.htm