This document provides information about diagnostic microbiology techniques. It discusses collecting specimens correctly and transporting them to the lab properly. It also summarizes various microbiology techniques like microscopy, culture, and sensitivity testing used to diagnose bacterial infections. These include staining methods like Gram stain and acid-fast stain to identify bacteria under the microscope. It also describes different types of culture media used for isolation, identification, and quantification of bacteria. Identification methods involve examining morphology, growth characteristics, and biochemical properties of bacteria. The document lists various non-cultural diagnostic techniques as well.

1. BLS 206: DIAGNOSTIC MICROBIOLOGY HOZA, A.S (2010) ROAD MAP …………….

4. Laboratory Diagnosis of Infection Ask the lab for a diagnosis, expecting a yes or no, but often end up with just a maybe…

6. Diagnosis of Bacterial Infection Patient Clinical diagnosis Haematology Biochemistry Non-microbiological investigations Radiology Sample Take the correct specimen Take the specimen correctly Label & package the specimen up correctly Appropriate transport & storage of specimen

7. A proper clinical assessment is essential for optimal use of laboratory services!

14. Diagnosis of Bacterial Infection culture on plates or in broth identification by biochemical or serological tests on pure growth from single colony microscopy Decolorise Counterstain Stain unstained or stained with e.g. Gram stain sensitivities Serodiagnosis DNA technologies by disc diffusion methods, breakpoints or MICs

22. I think it’s time for a short Break!! Oooh yeah!!!

23. Bacterial cultivation Part 1a Selective /differential media

41. OK guys, it’s the end of presentation, C U next time...!!