This document discusses biosurfactants, specifically rhamnolipids. It defines biosurfactants and notes that they are produced by microbes. Rhamnolipids are glycolipids produced by Pseudomonas aeruginosa bacteria. They are useful for their ability to lower surface tension and have applications in enhanced oil recovery, bioremediation, and more. The document outlines methods for rhamnolipid production and detection and reviews current and potential future applications. It concludes that biosurfactants are promising but still more expensive than chemicals and would benefit from further optimization and development.
viable but non-culturable microorganisms (VBNC).
WHAT IS A VBNC STATE?
CHARACTERISTICS OF BACTERIA IN VBNC STATE.
CONDITIONS STIMULATING VBNC STATE.
METHODS FOR DETECTION OF VBNC STATE.
Impact on public health.
Advantages of VBNC.
Disadvantages of VBNC state.
Conclusion.
Lactic acid bacteria (LAB),Lactic Acid Bacteria (LAB) or Lactics applications,Potential biomedical LAB applications,Exploitation of LAB,LAB as vaccine delivery vehicle,LAB as live vaccine,Recent developments ,Benefits of LAB based Vaccines,Future perspectives .
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this presentation describes about the bacteriocin and their mode of action. It also describes about its use along with the hurdle technology to enhance shelf life of food products.
viable but non-culturable microorganisms (VBNC).
WHAT IS A VBNC STATE?
CHARACTERISTICS OF BACTERIA IN VBNC STATE.
CONDITIONS STIMULATING VBNC STATE.
METHODS FOR DETECTION OF VBNC STATE.
Impact on public health.
Advantages of VBNC.
Disadvantages of VBNC state.
Conclusion.
Lactic acid bacteria (LAB),Lactic Acid Bacteria (LAB) or Lactics applications,Potential biomedical LAB applications,Exploitation of LAB,LAB as vaccine delivery vehicle,LAB as live vaccine,Recent developments ,Benefits of LAB based Vaccines,Future perspectives .
Bacteriocins as food preservatives 1 copy (1)JuhiMishra16
this presentation describes about the bacteriocin and their mode of action. It also describes about its use along with the hurdle technology to enhance shelf life of food products.
Cheese is a generic term for a diverse group of milk based food products.
Cheese consists of proteins and fat from milk, usually the milk of cows, buffalo, goats, or sheep.
It is produced by coagulation of the milk protein casein.
Science and technology of manipulating and improving microbial strains, in order to enhance their metabolic capacities for biotechnological applications, are referred to as strain improvement.
Industrial microorganisms and product formation PritamPaul43
this slide is about the industrial products whish are formed widly through out the globe. Here I have explained how peniceline is widly used and how we can make this more widly.
Thank you
Cheese is a generic term for a diverse group of milk based food products.
Cheese consists of proteins and fat from milk, usually the milk of cows, buffalo, goats, or sheep.
It is produced by coagulation of the milk protein casein.
Science and technology of manipulating and improving microbial strains, in order to enhance their metabolic capacities for biotechnological applications, are referred to as strain improvement.
Industrial microorganisms and product formation PritamPaul43
this slide is about the industrial products whish are formed widly through out the globe. Here I have explained how peniceline is widly used and how we can make this more widly.
Thank you
Isolation, Screening, and Characterization of Biosurfactant-Producing Microor...BRNSS Publication Hub
Introduction: Biosurfactants are amphiphatic in nature and are surface-active compounds produced by microorganisms. These molecules reduce interfacial surface tension between aqueous solutions and hydrocarbon mixtures. Unfortunately, oil spills and industrial discharges from petroleum-related industries have been identified as the major pollution sources. The hydrophobicity and low aqueous solubility of petroleum pollutant limit the biodegradation process. The features that make biosurfactants as an alternative to commercially synthesized surfactants are its low toxicity, higher biodegradability and, hence, greater environmental compatibility, better foaming properties, and stable activity at extreme pH, temperature, and salinity. Objective: Therefore, in this study, hydrocarbon-degrading bacteria were screened from petroleum-contaminated soil, characterized and optimization of the physical and nutrient parameters were done to enhance the production of biosurfactants. Results: Petroleum-contaminated soil was collected from different petrol pumps in Pune and screening was done on minimal salt medium media containing palm oil as carbon source using hemolytic activity, emulsification index, drop-collapse test, and oil displacement method. The most promising strain was isolated and identified using Bergey’s Manual of Determinative Biology and 16s rRNA sequencing and was found to be Staphylococcus epidermidis. The optimization of various parameters, namely temperature, pH, carbon, and nitrogen sources on growth, and biosurfactant production was studied. The highest biosurfactant production was obtained when MSS media contains sucrose (carbon source) and urea (nitrogen source) at pH 10 and temperature 55°C. The Fourier transform-infrared (FT-IR) analysis of purified biosurfactant indicated the presence of lipopeptide biosurfactant when compared with reference FT-IR spectra.
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT MICROBIAL BIO TRANSFORMATION WITH BIOCHEMICAL REACTIONS
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2. OUTLINEOUTLINE
• Definition of Biosurfactants
• Microbes that Produce Biosurfactants
• Rhamnolipids
• Production method
• Current Applications of Biosurfactants
• Future Applications of Biosurfactants
• Conclusion
3. WHAT IS A BIOSURFACTANT?WHAT IS A BIOSURFACTANT?
4. PROBLEM DESCRIPTION & MARKET NEEDPROBLEM DESCRIPTION & MARKET NEED
Nowadays surfactants are one of the most
important substances for many fields of industry
- pharmacy, food industry, design of washing
agents, petroleum industry, agriculture,
environmental protection and remediation
An excessive use of chemical surfactants leads to
technogenic load on environment, flora and
fauna, affects on food products
Biosurfactants can satisfy the needs of the
modern market in natural products, particularly
surface-active substances of new generation
(effective and ecologically safe)
7. RHAMNOLIPIDSRHAMNOLIPIDS
Rhamnolipids are naturally occurring glycolipid produced
commercially by the Pseudomonas aeruginosa species of
bacteria. There are two types:
1.mono- rhamnolipids
2.di- rhamnolipids
8. PHYSIOLOGICAL FUNCTIONS AND ROLESPHYSIOLOGICAL FUNCTIONS AND ROLES
OF RHAMNOLIPIDSOF RHAMNOLIPIDS(Why interest for commercial use?)(Why interest for commercial use?)
• RLs promote the uptake and
biodegradation of poorly soluble
substrates
• RLs as immune modulators and virulence
factors
• RLs as antimicrobials
• RLs in surface motility
• RLs in biofilm development
11. Use of inexpensive raw materials for the production of biosurfactantsUse of inexpensive raw materials for the production of biosurfactants
by various microbial strainsby various microbial strains
12. Yields of rhamnolipids related to biomass (Yp/x) for fermentations by
Pseudomonas aeruginosa with different C/N ratios.
15. INDUSTRIAL APPLICATIONSINDUSTRIAL APPLICATIONS
Industry Application Role of biosurfactants
Petroleum Enhanced oil recovery Lowering of interfacial tension, dissolving
of oil
Environmental Bioremediation Lowering of interfacial tension
Food Emulsification and
de-emulsification
Solubilizer, demulsifier, suspension,
wetting, foaming
Bioprocessing Downstream processing Microemulsions, biotransformation,
Cosmetic Health and beauty products Foaming agents, solubilizers, wetting
agents, cleansers
Biological Microbiological Cell–cell competition, plant and animal
pathogenesis
Pharmaceutical and
therapeutics
Antibacterial, antifungal
Agricultural Biocontrol Parasitism, antibiosis, competition,
16. MICROBIAL ENHANCED OIL RECOVERYMICROBIAL ENHANCED OIL RECOVERY
A series of microscopic
photos shows the Process
with live microbes
surrounding a droplet of
crude oil, distorting its
shape and finally causing
a smaller droplet to break
away.
http://www.titanoilrecovery.com/pdfs/TitanBrochure.pdf
17. CASE STUDY: BEATRICE FIELD, ENGLANDCASE STUDY: BEATRICE FIELD, ENGLAND
• Field was scheduled
to be abandoned
in‘95-’96
• British Petroleum
applied MEOR in1995
• There was a 25%
increase over the 3-
year production
schedule
http://www.titanoilrecovery.com/pdfs/TitanBrochure.pdf
19. CURRENT DIRECTIONS OF R&DCURRENT DIRECTIONS OF R&D
BioengineeringBioengineering
Biosynthesis and studying of microbial enzymes and their compositions.
Investigation of synthesis of biosurfactants, their properties and application
in biomedicine, agriculture, food, pharmaceutical and cosmetic industry.
Chemistry/ MaterialsChemistry/ Materials
Creation and investigation of new polymeric materials.
ChemistryChemistry
Investigation of catalytic oxidation of hydrocarbon derivatives.
Chemical/Environmental EngineeringChemical/Environmental Engineering
Development of methods of bioremediation of water and soil.
Emulsification of HC’s (adherence), lowering interfacial tension, metal
sequestration, dispersion, foaming agent,
Monitoring of petroleum-contaminated water and soil.
20. CURRENT BIOREMEDIATION METHODSCURRENT BIOREMEDIATION METHODS
• In situ soil flushing
• Ex situ washing
• Heavy metal sequestration
Rhamnolipids are mostly used.
22. ADVANTAGESADVANTAGES
Recovery of pure Rhamnolipids without
antifoam contamination for use in ecological
washing and cleaning agents or cosmetics
Reuse of cells by repeated batch-process
leads to an economic and high-yield
production of Rhamnolipids
Use of renewable sources (e.g. vegetable oils)
instead of petroleum-based chemicals
Possibility to recover rhamnose sugar by
direct hydrolysis of the crude product, e.g.
for furaneol synthesis or use as flavors
23. CONCLUSIONCONCLUSION
Lower yields.
Still more expensive than chemical surfactants.
Process and production optimization needs to be improved.
Cheaper substrates + optimal growth and production conditions +
novel efficient multi-step downstream + recombinant and mutant
hyper producing microbial strains
Future applications as:
- fine specialty chemicals
- biological control agents,
- new generation molecules for pharmaceutical, cosmetic and health
care industries.
Ecofriendly synthesis of silver nanoparticles and stabilizer before
addition.
Ecofriendly product for flocculation and dispersion of high solid
contents of micro particles.
24. REFERENCESREFERENCES
Olivera, N.L., Commendatore, M.G., Moran, A.C. and Esteves J.L. 2000.
Biosurfactant-enhanced degradation of residual hydrocarbons from ship bilge
wastes, Journal of Industrial Microbiology and Biotechnology 25: 70-73
Mulligan, C.N., Environmental applications for biosurfactants. Environmental
Pollution 133: 183–198
Schippers, C., Gener, K., Muller, T. and Scheper, T. (2000) Microbial degradation
of phenanthrene by addition of a sophorolipid mixture, Journal of
Biotechnology 83:189-198
Tecon, R. and van der Meer J.R. .2009. Effect of two types of biosurfactants on
phenanthrene availability to the bacterial bioreporter Burkholderia sartisoli
strain RP037. App. Microbiol Biotech- online DOI 10.1007/s00253-009-2216-0
Muthusamy, K., Gopalakrishnan S., Ravi, T.K. and Sivachidambaram, P. 2008.
Biosurfactants: Properties, commercial production and application. Review
Article. Current Science 94- 6: 736-747
Kosaric, N., 2001. Biosurfactants and their application for soil bioremediation.
Food Technol. Biotechnol., 39:295-304.
Qinhong Wang, Xiangdong Fang, Baojun Bai, Xiaolin Liang, Patrick J. Shuler,
William A. Goddard III, Yongchun Tang Received 23 January 2007; revision
received 5 April 2007; accepted 6 April 2007 DOI 10.1002/bit.21462