This document describes biosafety level one, which involves handling well-defined agents with minimal threat to people, animals, and the environment. Laboratories at this level have open bench tops, sinks, windows, and doors, and are not separated from other areas of the building. Standard practices include hand washing, limiting access while working, decontaminating work surfaces and waste, and using personal protective equipment like lab coats and gloves. Decontamination is achieved through disinfection or autoclaving, and all activities are supervised by trained personnel following standard procedures.

1. BIOSAFETY LEVELS ONE
MUTHEMBA KIPRUTO SAMUEL

2. B.S.L ONE:
Basic lab’s handling,
Well defined and characterized agents with no
or minimal threat to; Immunocompetent
Persons, Environment, Animals and Plants.
e.g. B. thuringiensis AND E.coli K12.
 In water testing labs, high school, colleges and basic
research institutions

3. WORK AREA:
Open bench tops impervious to water.
Sinks for washing hands/apparatus.
Windows opening with fly screens.
Doors for access/limiting access.
Generally not separated from main-building.
No special safety needed.

4. S.M.P:
Standard microbiological practices are observed
 Personal hygiene i.e. hand washing with liquid saops
 Mouth pipetting prohibited
 Work surface decontaminated before and after work
 Access limited while working
 Wastes decontaminated before disposal
 Biohazards signs are posted
 Spills decontaminated immediately
 Personal protective gadgets used i.e. lab coat and gloves

5. DECONTAMINATION:
By,
Treating with disinfectants.
(Alcohol/Bleach)
Steam autoclaving.

6. PERSONNEL:
Experiments performed by trained personnel
Use of standard operating procedures/protocols
Proper protective gears like white lab coats and gloves
All the activities of the laboratory supervised by a well trained
microbiologist or biologist.