2. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
CONTENTS
Introduction
Need &
Application
Production
,Extraction,
Future prospects
References
3. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
INTRODUCTION
Biopolymers are polymeric biomolecules which contain
monomeric units that are covalently bonded to form
larger molecules.
They are solid ,higher molecular weight non-metallic
compounds
Biopolymers are biological macromolecule which can
be derived from renewable sources such as plants, animal
and living organisms.
Important property of biopolymers is
‘Biodegradability’
4. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
There are three main classes of biopolymers based on
the differing monomeric units used and the structures of
biopolymers formed:
Polynucleotide
Polypeptides
Polysaccharides
Biopolymers and plastic are similar in appearance
Plastic product Biopolymer product
5. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
Need 0f Biopolymers
Plastics materials are used world wide today its many
application. Most of these plastics are derived from
petroleum and they are not degradable.
Due to unorganized and improper disposal non degradable
plastics causing harm to the environment.
As per a survey conducted by Central Pollution Control
Board (CPCB) in 60 cities of the country, the plastic waste
generation is estimated to 15,000.0 tonnes per day.
To protect the needs of future generation from hazardous
environment, biopolymers is better option.
7. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
Properties Synthetic
polymers
Natural
biopolymers
Main Source Petroleum And Gas Biomass
Biodegradability rate Slow fast
Structure Random Well Defined
Chemical Backbone
Structure
Mostly Carbon Carbon, Oxygen And
Nitrogen
Source NOT Renewable
Source
Renewable Source
Needs of Biopolymers
8. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
Coating
Water treatment
Rubber,Plastic
Medicine
Fiber , Paper
Oil industry
Biomedical
APPLICATIONS
9. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
POLYHYDROXYBUTYRATE (PHB)
PHBs are short chain length Biopolymers of
Polyhydroxyalkanoates (3-5 carbons)
Produced in nature by bacterial fermentation of sugars
and lipids
Polyesters accumulated inside microbial cells as
carbon and energy source storage
Produced under the conditions of :
• Low limiting nutrients (P, S, N, O)
• Excess carbon source
Found in Alcaligenes eutrophus and accumulated
intracellularly as granules
10. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
PROPERTIES OF PHB
Some are stiff and brittle
Some are rubbery and mouldable
They act like polyesters thermoplastic properties similar to
polypropylene and polyethylene.
Moisture resistant, water insoluble, optically pure.
Excellent flexibility
Degrades at 180ͦ C without releasing any toxic gases
11. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
Production and types of mechanisms
Microbial production
Isolation from totally natural sources.
Extraction from plants and algae.
.
12. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
Microorganism
& culture
Condition
Fermentation
Process
Extraction of
Biopolymers
Characterization of
Biopolymers
PRODUCTION
PROCESS
13. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
SOURCES AND MICROORGANISMS FOR PHB PRODUC
Sr.No Source Strain
References
number
1 Sugar Molasses
Alcaligenes
eutrophus
1
2
Organic Waste
water Pseudomonas strain
2
3
Food industrial
waste
Pseudomonas
Fluorescens
3
4 Vegetable waste
Alcaligenes
eutrophus
4
14. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
FUTURE PROSPECTS
We will use alternative carbon sources such as -
pineapple peel, palm sugar, beet peel, coconut juice
,potato peel , orange peel.
Organic waste water,
Vegetable waste.
We Will perform fermentation process on laboratory scale
level.
Analytical techniques –
• Fourier Transform-Infrared Spectroscopy(FTIS)
• Differential Scanning Colorimeter.(DSC)
15. SCOE
DEPARTMENT OF BIOTECHNOLOGY, PUNE 2016-17
REFERENCES
1. Ratthadaporn Phathipchotikun1, Phakphimol Piwpan1, Adisak Jaturapiree2 and Phimchanok
Jaturapiree1,* " Polyhydroxybutyrate (PHB) production by Alcaligenes eutrophus NCIMB 11599
from low-cost substrate as carbon source " Department of Biotechnology, Faculty of Engineering
and Industrial Technology, Silpakorn University, Muang, Nakhon Pathom 73000, Thailand 2
Chemistry Program, Faculty of Science, Nakhon Pathom Rajabhat University, Muang, Nakhon
Pathom 73000, Thailand
2. Sajida Munir* and Nazia Jamil. "Characterization of Polyhydroxyalkanoates Produced by
Contaminated Soil Bacteria using Wastewater and Glucose as Carbon Sources." Tropical Journal of
Pharmaceutical Research Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria
3. M.N.Priyadharshini1*, K. Priyadharshini1, A. Priyadharshni1, A. Akilandeeswari" Production and
characterisation of biopolymer usingPseudomonas fluorescens (pf 01)" 1Department of Industrial
Biotechnology, Government College of Technology, Coimbator Department of Biotechnology,
Periyar Maniammai University, Thanjavur
4. Paola Di Donatoa,b, Ilaria Finorea, Gianluca Anzelmoa, Licia Lamaa , Barbara Nicolausa ,
Annarita Poli " Biomass and Biopolymer Production using Vegetable Wastesas Cheap Substrates
for Extremophiles” aNational Research Council of Italy, Institute of Biomolecular Chemistry,Via
Campi Flegrei, 34, (80078) Pozzuoli (NA) - Italy University of Naples Parthenope, Department of
Sciences and Technology, Centro Direzionale Isola Napoli,Italy
5. Anteneh Getachew1* and Fantahun Woldesenbet2" Production of biodegradable plastic
by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material"
Getachew and Woldesenbet BMC Res Notes
