This document discusses biodiversity hotspots and threats to biodiversity. It defines biodiversity hotspots as regions that are exceptionally rich in endemic species yet threatened by habitat loss. Two criteria are outlined to qualify as a hotspot: containing at least 1,500 endemic plant species and having lost at least 70% of primary vegetation. 25 global hotspots are listed, including the Eastern Himalayas and Western Ghats in India. Major threats include habitat loss, poaching, and human-wildlife conflicts. Conservation approaches of in situ and ex situ are described, along with examples like protected areas, gene banks, and projects like Tiger and Elephant.