Bio magnification, also known as biological magnification, refers to the increasing concentration of a substance like pesticides or heavy metals as it moves up the food chain. Substances become more concentrated in the tissues of organisms at higher trophic levels due to the persistence of these chemicals in the environment and their low excretion rates from organisms' bodies. Two main groups of substances are prone to biomagnification - persistent organic pollutants like PCBs and metals, both of which are not easily degraded. Predator species face greater risks from biomagnification as the concentration of toxins is highest in their tissues, being at the top of food webs and consuming large amounts of contaminated prey.