Biomagnification refers to the increasing concentration of a pollutant that occurs as it moves up the food chain from lower to higher trophic levels. It occurs when substances are persistent, not easily broken down, and soluble in fats. As a result, organisms at the top of the food chain experience greater harm from toxins than those lower down. Examples include the accumulation of mercury in large predatory fish, which can then pose health risks to humans who consume such fish. The process involves pollutants being absorbed and concentrated at each level of the food chain.