This document discusses the use of bacteriophages to treat various bacterial infections caused by E. coli, Salmonella, Shigella, Staphylococcus, and Streptococcus. It provides information on the classification and pathogenic characteristics of these bacteria. Bacteriophages target specific bacteria and can be used as alternatives to antibiotics to treat infections and prevent the spread of disease. The document focuses on using bacteriophages therapeutically and for prophylaxis against various foodborne illnesses and infections.
phage therapy is the use of bacteriophages to kill pathogenic bacterial cells. Bacteriophages are bacterial parasites that invade bacterial cells and engulf them like blue whale fish kills euphausiids and copepodsand in sea .
Phage therapy (PT) is also called bacteriophage therapy. It uses viruses to treat bacterial infections. Bacterial viruses are called phages or bacteriophages. They only attack bacteria; phages are harmless to people, animals, and plants. Bacteriophages are the natural enemies of bacteria.
phage therapy is the use of bacteriophages to kill pathogenic bacterial cells. Bacteriophages are bacterial parasites that invade bacterial cells and engulf them like blue whale fish kills euphausiids and copepodsand in sea .
Phage therapy (PT) is also called bacteriophage therapy. It uses viruses to treat bacterial infections. Bacterial viruses are called phages or bacteriophages. They only attack bacteria; phages are harmless to people, animals, and plants. Bacteriophages are the natural enemies of bacteria.
PSP 3 - Literature review presentation of Acinetobacter baumannii and relevant virulence, symptoms, diagnosis, antibiotic resistance, available and potential future treatments and how this review links to further study and a potential career.
It has been developed for the detection, enumeration & identification of bacteria & yeasts in clinical specimens.
It is an instrument used for automatic computer-assisted identification of bacteria
It mainly involves staining, motility test, cultural characteristics, a series of biochemical tests.
The automatic bacteria identification system automatically identifies the bacteria in very short time.
Acinetobacter: Awakening of a sleeping demonShyam Mishra
Acinetobacter is an emerging pathogen associated with several infections, in particular hospital-acquired infections. It is notorious for its multidrug resistance property. It is a great nuisance for the clinicians, microbiologists and a subject of great research for the scientists.
PSP 3 - Literature review presentation of Acinetobacter baumannii and relevant virulence, symptoms, diagnosis, antibiotic resistance, available and potential future treatments and how this review links to further study and a potential career.
It has been developed for the detection, enumeration & identification of bacteria & yeasts in clinical specimens.
It is an instrument used for automatic computer-assisted identification of bacteria
It mainly involves staining, motility test, cultural characteristics, a series of biochemical tests.
The automatic bacteria identification system automatically identifies the bacteria in very short time.
Acinetobacter: Awakening of a sleeping demonShyam Mishra
Acinetobacter is an emerging pathogen associated with several infections, in particular hospital-acquired infections. It is notorious for its multidrug resistance property. It is a great nuisance for the clinicians, microbiologists and a subject of great research for the scientists.
Bacteriophage vectors
Bacteriophage
WHY BACTERIOPHAGE AS A VECTOR?
M13 phage
Genome of m13 phage
Life cycle and dna replication of m13
CONSTRUCTION M13 AS PHAGE VECTOR
M13 MP 2 vector
M13MP7 VECTOR
Selection of recombinants
Lambda replacement vectors
LAMBDA EMBL 4 VECTOR
P1 PHAGE
GENOME OF P1 PHAGE
P1 PHAGE AS VECTOR
P1 phage vector system
A Hlavay József Országos Környezettudományi és Műszaki Diákkonferenciát 2015. április 17-18 között 12. alkalommal rendezték meg Veszprémben, ahol két boronkays diákunk is elindult.
A „Saját kutatómunka bemutatása” kategóriában indult Boros Zsófi és Liebhardt Ági (10.G). A pontosan 10 perces előadás után 5 perces vitában kellett megvédeniök állításaikat. Zsófi és Ági nem csupán egy derűs, tartalmas és jól érthető előadást tartott „Fokhagyma a mini gyógyszergyár” címen az elmúlt évben végzett kutatómunkájáról, hanem a vitában fantasztikus biztonsággal és nagyon nagy szerénységgel bizonyították, hogy a témában elméletileg is és a gyakorlat szempontjából is otthonosan mozognak. A zsűri nagyon biztatva őket a kutatás folytatására a legjobbnak ítélte őket.
Felkészítő tanáruk: Dr. Tóth Eszter volt.
خشکسالی
پديده محيطي و بخش جدايي ناپذير اقليم مناطق مختلف
اثرات؛ بر بخش های اقلیمی، کشاورزی، زیست محیطی، منابع آب و پیامدهای اقتصادی – اجتماعی
انواع خشکسالی:
هواشناسی، کشاورزی، هیدرولوژیکی، اجتماعی - اقتصادی
ويژگيها:
رخداد مکرر و حرکت آرام و خسارات سنگين اقتصادی - اجتماعی و زیست محیطی
Enterobacteriaceae is a large family of Gram-negative bacteria. It was first proposed by Rahn in 1936, and now includes over 30 genera and more than 100 ...
Microbiology of E coli giving basic of Escherichia coli, its morphology, cultural and biochemical characteristics, Antigenic character, pathogenesis, laboratory diagnosis, prevention and control
These bacteria make spores, which act like protective coatings that help the bacteria survive. Under certain conditions, such as when food is kept at an unsafe temperature (between 40°F–140°F), C. perfringens can grow and multiply. After someone swallows the bacteria, it can produce a toxin (poison) that causes diarrhea.
Common sources of C. perfringens infection include meat, poultry, gravies, and other foods cooked in large batches and held at an unsafe temperature. Outbreaks tend to happen in places that serve large groups of people, such as hospitals, school cafeterias, prisons, and nursing homes, and at events with catered food. C. perfringens outbreaks occur most often in November and December. Many of these outbreaks have been linked to foods commonly served during the holidays, such as turkey and roast beef.
Anyone can get food poisoning from C. perfringens. Young children and older adults are at higher risk for severe illness.
Bacillary dysentery is a gastrointestinal disease. Bacillary means related to bacteria, and dysentery is severe diarrhea containing blood or mucus. With bacillary dysentery, a bacterial infection becomes more invasive and severe, causing inflammation in the intestines. Symptoms can range from mild to life-threateningDysentery is an infection of the intestines that causes diarrhoea containing blood or mucus. Other symptoms of dysentery can include: painful stomach cramps. feeling sick or being sick (vomiting)Transmission is fecal-oral and is remarkable for the small number of organisms that may cause disease (10 ingested organisms cause illness in 10% of volunteers, and 500 organisms cause disease in 50% of volunteers). Shigella bacteria invade the intestinal mucosal cells but do not usually go beyond the lamina propria. Dysentery is caused when the bacteria escape the epithelial cell phagolysosome, multiply within the cytoplasm, and destroy host cells. Shiga toxin causes hemorrhagic colitis and hemolytic-uremic syndrome by damaging endothelial cells in the microvasculature of the colon and the glomeruli, respectively. In addition, chronic arthritis secondary to S. flexneri infection, called reactive arthritis, may be caused by a bacterial antigen; the occurrence of this syndrome is strongly linked to HLA-B27 genotype, but the immunologic basis of this reaction is not understoodSpecimen: Fresh stool is collected.
Culture: Specimen is inoculated on selective media like MacConkey's agar, DCA, XLD agar. Selenite F broth(0.4%) is used as enrichment medium which permits the rapid growth of enteric pathogens while inhibiting the growth of normal flora like E. coli for 6–8 hours. Subculture is done on the solid media from selenite F broth. All the solid media are incubated at 37 degrees for 24 hours.
Cultural characteristics: Colorless (NLF) colonies appear on MacConkey's agar which are further confirmed by gram staining, hanging drop preparation and biochemical reactions.Dysentery is initially managed by maintaining fluid intake using oral rehydration therapy. If this treatment cannot be adequately maintained due to vomiting or the profuseness of diarrhea, hospital admission may be required for intravenous fluid replacement. Ideally, no antimicrobial therapy should be administered until microbiological microscopy and culture studies have established the specific infection involved. When laboratory services are not available, it may be necessary to administer a combination of drugs, including an amoebicidal drug to kill the parasite and an antibiotic to treat any associated bacterial infection.
Anyone with bloody diarrhea needs immediate medical help. Treatment often starts with an oral rehydrating solution—water mixed with salt and carbohydrates—to prevent dehydration. (Emergency relief services often distribute inexpensive packets of sugars and mineral salts that can be mixed with clean water and used to restore lifesaving fluids in dehydrated child
Gastroenteritis
One of the primary concerns related to gastrointestinal (GI)infection, regardless of the cause, is dehydration, which is the second leading cause of worldwide morbidity and mortality.
Worldwide, dehydration is especially problematic for children younger than age 5.
However, the highest rate of death occurs among the elderly.
Rehydration is the foundation of therapy for GI infections, and oral rehydration therapy (ORT) is usually preferred.
Gastroenteritis, also known as infectious diarrhea and gastro, is inflammation of the gastrointestinal tract—the stomach and intestine.
Diarrhea is defined as the production of stool of abnormally loose consistency, usually associated with excessive frequency of defecation and excessive stool output.
Acute Diarrhea lasts 14 days or less.
Persistent Diarrhea lasts more than 14 days.
Chronic Diarrhea lasts more than 1 month.
Protection of humans during long space flight. using cannabis to reduce biol...Dmitri Popov
Protection of humans during long space flight. using cannabis to reduce biological consequences of high doses of radiation, treat stress, anxiety, and depression Associated with Long-term Space Flight to Mars.
ANTIOXIDANTS AND POTASSIUM FERROCYANIDE, APROPHYLACTIC AND THERAPEUTIC MIXTU...Dmitri Popov
ANTIOXIDANTS AND POTASSIUM FERROCYANIDE, APROPHYLACTIC AND THERAPEUTIC MIXTURE COMPRISING THIS COMPOUND AND THE USE THEREOF FOR DECORPORATION OF RADIOCESIUM IN SUBJECTS AFFECTED BY NUCLEAR RADIATION
Implications for Immunotherapy of Acute Radiation Syndromes. Part 2.Dmitri Popov
Research Proposal: Implications for Immunotherapy of Acute Radiation Syndromes. Part 2.
Dmitri Popov
Full-text available · Research Proposal · Feb 2017
File name: Implications for Immunotherapy of ARS. Part 2.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
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MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
3. Bacteriophage
Bacteriophage, dysenteri, polyvalente 1,2,3,4,6 serotypes.
Bacteriophage for therapy and prevention of dysentery.
Shigellosis, also known as bacillary dysentery or Marlow syndrome, in its most severe manifestation, is
a foodborne illness caused by infection by bacteria of the genus Shigella.
Shigellosis rarely occurs in animals other than humans
Shigella sonnae, flexneri
Shigella species are classified by four serogroups:
Serogroup A: S. dysenteriae (15 serotypes)
Serogroup B: S. flexneri (six serotypes)
Serogroup C: S. boydii (19 serotypes)
Serogroup D: S. sonnei (one serotype)
4. Bacteriophage therapy.
Groups A–C are physiologically similar; S. sonnei (group D) can be differentiated on the basis
of biochemical metabolism assays.
Three Shigella groups are the major disease-causing species: S. flexneri is the most frequently
isolated species worldwide, and accounts for 60% of cases in the developing world; S.
sonnei causes 77% of cases in the developed world, compared to only 15% of cases in the
developing world; and S. dysenteriae is usually the cause of epidemics of dysentery,
particularly in confined populations such as refugee camps.
Each of the Shigella genomes includes a virulence plasmid that encodes conserved primary
virulence determinants. The Shigella chromosomes share most of their genes with those of E.
coli K12 strain MG1655.
5. Bacteriophage
Bacteriphage to salmonella group ABCDE
Salmonella Serogroups A,В,С,D,E.
Salmonellosis is an infection caused by Salmonella bacteria. Most people infected
with Salmonella develop diarrhea, fever, vomiting, and abdominal cramps 12 to 72
hours after infection. In most cases, the illness lasts four to seven days, and most
people recover without treatment. In some cases, the diarrhea may be so severe that
the patient becomes dangerously dehydrated and must be hospitalized.
Salmonellosis is a major cause of bacterial enteric illness in both humans and animals.
Each year an estimated 1.4 million cases of salmonellosis occur among humans in the
United States.
www.researchgate.net850 × 608Search by image Figure 1. General overview of the
current classification of Salmonella enterica . doi:10.1371/journal.ppat.1002776.g001
7. Bacteriophage
Bacteriophage to Salmonella enterica typhi.
Therapy and prophylaxis.
Worldwide, typhoid fever affects roughly 17 million people annually, causing nearly 600,000 deaths. The
causative agent, Salmonella enterica typhi (referred to as Salmonella typhi.
Infection of S. typhi leads to the development of typhoid, or enteric fever. This disease is characterized by
the sudden onset of a sustained and systemic fever, severe headache, nausea.
Other symptoms include constipation or diarrhea, enlargement of the spleen, possible development of
meningitis, and/or general malaise.
Untreated typhoid fever cases result in mortality rates ranging from 12-30% while treated cases allow for
99% survival.
http://web.uconn.edu/mcbstaff/graf/Student%20presentations/Salmonellatyphi/Salmonellatyphi.html
8. Bacteriophage
Bacteriophage to Stapyilococcus aureus.
Staphylococcus aureus is a gram-positive coccal bacterium that is a member of the Firmicutes,
and is frequently found in the nose,respiratory tract, and on the skin. It is often positive
for catalase and nitrate reduction. Although S. aureus is not always pathogenic, it is a
common cause of skin infections such as abscesses, respiratory infections such as sinusitis,
and food poisoning. Pathogenic strains often promote infections by producing
potent protein toxins, and expressing cell-surface proteins that bind and inactivate
antibodies. The emergence of antibiotic-resistant strains of S. aureus such as methicillin-
resistant S. aureus (MRSA) is a worldwide problem in clinical medicine.
https://en.wikipedia.org/wiki/Staphylococcus_aureus
https://www.google.ca/search?q=staphylococcus+classification&espv
10. Bacteriophage to staphylococcus.
Staphylococcus can cause a wide variety of diseases in humans and animals through
either toxin production or penetration. Staphylococcal toxins are a common cause of
food poisoning, for they can be produced by bacteria growing in improperly stored
food items. The most common sialadenitis is caused by staphylococci, as bacterial
infections.
Bacteriophage to staph infections
Treatment and prevention of purulent infections of the skin , mucous membranes,
coagulase-negative staphylococci caused by staphylococci , as well as dysbacteriosis.
Bacteriophage is used to treat cystitis , cholecystitis , acute tonsillitis , enterocolitis ,
and others .
14. Bacteriophage
Bacteriophage to Echerechia coli.
Бактериофаг коли,
Энтеропатогенная Echerichia coli.
Escherichia coli (/ˌɛʃəˈrɪkiə ˈkoʊlɪ/ Anglicized to /ˌɛʃəˈrɪkiə ˈkoʊlaɪ/; commonly abbreviated E.
coli) is a gram-negative, rod-shapedbacterium that is commonly found in the
lower intestine of warm-blooded organisms (endotherms).
Most E. coli strains are harmless, but some serotypes are pathogenic and can cause serious
infections or food poisoning in humans, and are occasionally responsible for product
recalls
15. Bacteriophage.
Phage therapy—viruses that specifically target pathogenic bacteria—has been
developed over the last 80 years, primarily in the former Soviet Union, where it was
used to prevent diarrhea caused by E. coli.
Presently, phage therapy for humans is available only at the Phage Therapy Center in
the Republic of Georgia and in Poland.
However, on January 2, 2007, the United States FDA gave Omnilytics approval to
apply its E. coli O157:H7 killing phage in a mist, spray or wash on live animals that will
be slaughtered for human consumption.
The enterobacteria phage T4, a highly studied phage, targets E. coli for infection.
16. Bacteriophage.
E.coli infections:
Pathogenic E.coli strains can be categorized based on elements that can elicit an
immune response in animals, namely:
O antigen: part of lipopolysaccharide layer
K antigen: capsule
H antigen: flagellin
For example, E.coli strain EDL933 is of the O157:H7 group
17. Bacteriophage therapy.
Enterotoxigenic E. coli (ETEC)
causative agent of diarrhea (without fever) in humans, pigs, sheep, goats, cattle, dogs, and horses.
ETEC uses fimbrial adhesins (projections from the bacterial cell surface) to bind enterocyte cells in
the small intestine. ETEC can produce two proteinaceous enterotoxins: The larger of the two
proteins, LT enterotoxin, is similar to cholera toxin in structure and function.
The smaller protein, ST enterotoxin causes cGMP accumulation in the target cells and a subsequent
secretion of fluid and electrolytes into the intestinal lumen.
ETEC strains are non-invasive, and they do not leave the intestinal lumen. ETEC is the leading bacterial
cause of diarrhea in children in the developing world, as well as the most common cause of traveler's
diarrhea. Each year, ETEC causes more than 200 million cases of diarrhea and 380,000 deaths, mostly
in children in developing countries.
https://en.wikipedia.org/wiki/Pathogenic_Escherichia_coli#Serotypes
18. Bacteriophage therapy.
Enteropathogenic E. coli (EPEC) , causative agent of diarrhea in humans, rabbits, dogs,
cats and horses.
Like ETEC, EPEC also causes diarrhea, but the molecular mechanisms of colonization
and aetiology are different. EPEC lack ST and LT toxins, but they use an adhesin known
as intimin to bind host intestinal cells. This virotype has an array of virulence factors
that are similar to those found in Shigella, and may possess a shiga toxin. Adherence
to the intestinal mucosa causes a rearrangement ofactin in the host cell, causing
significant deformation. EPEC cells are moderately invasive (i.e. they enter host cells)
and elicit an inflammatory response. Changes in intestinal cell ultrastructure due to
"attachment and effacement" is likely the prime cause of diarrhea in those afflicted
with EPEC. https://en.wikipedia.org/wiki/Pathogenic_Escherichia_coli#Serotypes
19. Bacteriophage therapy.
Enteroinvasive E. coli (EIEC) . EIEC infection causes a syndrome that is identical
to shigellosis, with profuse diarrhea and high fever.
The most infamous member of this virotype is strain O157:H7, which causes bloody
diarrhea and no fever. EHEC can cause hemolytic-uremic syndrome and sudden
kidney failure. It uses bacterial fimbriae for attachment (E. coli common pilus, ECP),is
moderately invasive and possesses a phage-encoded shiga toxin that can elicit an
intense inflammatory response.
https://en.wikipedia.org/wiki/Pathogenic_Escherichia_coli#Serotypes
20. Bacteriophage therapy.
Enteroaggregative E. coli (EAEC)
so named because they have fimbriae which aggregate tissue culture cells, EAEC bind
to the intestinal mucosa to cause watery diarrhea without fever. EAEC are non-
invasive. They produce a hemolysin and an ST enterotoxin similar to that of ETEC.
21. Bacteriophage therapy
Adherent-Invasive E. coli (AIEC)
AIEC are able to invade intestinal epithelial cells and replicate intracellularly. It is likely
that AIEC are able to proliferate more effectively in hosts with defective innate
immunity. They are associated with the ileal mucosa in Crohn's disease.
https://en.wikipedia.org/wiki/Pathogenic_Escherichia_coli#Serotypes
22. Bacteriophage therapy.
Enterohemorrhagic E. coli (EHEC) .
The most infamous member of this virotype is strain O157:H7, which causes bloody
diarrhea and no fever. EHEC can cause hemolytic-uremic syndrome and sudden
kidney failure. It uses bacterial fimbriae for attachment (E. coli common pilus,
ECP),[19] is moderately invasive and possesses a phage-encoded shiga toxin that can
elicit an intense inflammatory response.
23. Bacteriophage
Bacteriophage to Pseudomonas aeruginosa.
Pseudomonas aeruginosa is a common Gram-negative, rod-shaped bacterium that
can cause disease in plants and animals, including humans.
A species of considerable medical importance, P. aeruginosa is a prototypical
"multidrug resistant (MDR) pathogen" recognised for its ubiquity, its intrinsically
advanced antibiotic resistance mechanisms, and its association with serious illnesses
– especially nosocomial infections such as ventilator-associated pneumonia and
various sepsis syndromes.
24. Bacteriophage
Bacteriophage to Klebsiella pneumoniae.
Klebsiella pneumoniae is a Gram-negative, nonmotile, encapsulated, lactose-
fermenting, facultative anaerobic, rod- shapedbacterium.
Klebsiella appears as a mucoid lactose fermenter on MacConkey agar.
Although found in the normal flora of the mouth, skin, and intestines, it can cause
destructive changes to human and animal lungs if aspirated (inhaled), specifically to
the alveoli (in the lungs) resulting in bloody sputum. In the clinical setting, it is the
most significant member of the Klebsiella genus of Enterobacteriaceae. K.
oxytoca and K. rhinoscleromatis have also been demonstrated in human clinical
specimens. In recent years, Klebsiella species have become important pathogens
in nosocomial infections.
27. Bacteriophage
Pyo bacteriophage, complex (Секстафаг)
Bacteriophage to P. aeruginosa, P. mirabilis, P. vulgaris,
K. pneumoniae, Staphylococcus, Enterococcus,
энтеропатогенная E. coli, K. oxytoca
28. Literature
BACTERIOPHAGE BASED PREPARATIONS: A BRIEF SURVEY OF CURRENT STATE
AND FUTURE DEVELOPMENT
I.V. Krasilnikov, K.A. Lysko, E.V. Otrashevskaya, A.K. Lobastova
Federal State Unitary Company ”Microgen” Scientific Industrial Company for
Immunobiological Medicines” of the Ministry of Health and
Social Development of the Russian Federation, Moscow
29. Literature
Wright, C.H. Hawkins et al. A controlled clinical trial of a
therapeutic bacteriophage preparation in chronic otitis due to
antibiotic-resistant Pseudomonas aeruginosa; a preliminary
report of efficacy // Clinical Otolaryngology. – 2009. – Vol. 34,
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Ackermann H. – W., Dubow M. S.
Viruses of prokaryotes, vol. I. General properties of bacteriophages. – CRC
Press: Boca Raton, 1987. 231 p.
30. Literature
Weber-Dabrowska, Zimecki M., Kruzel M. et al. Alternative
therapies in antibiotic-resistant infection // Advances in Medical
Sciences. – 2006. – Vol. 51. – P. 242–244.
Abedon, S. T. (1994). Lysis and the Interaction between Free Phages and Infected
Cells, p. 397-405. In J. D. Karam (ed.), Molecular Biology of Bacteriophage T4.
American Society for Microbiology, Washington, DC.
31. Literature
Barrow, P. A. and J. S. Soothill. (1997). Bacteriophage Therapy and Prophylaxis:
rediscovery and renewed assessment of the potential. Trends Microbiol 5:268-271
Burnet, F. and M. McKie (1929). Observations on a permanently lysogenic strain of B.
enteridis gaerther. Austral. J. Exptl. Biol. Med. Sci. 6: 277-284.]
32. Literature
Doermann, A. D. (1948). Lysis and lysis inhibition with Escherichia coli bacteriophage.
J. Bacteriol. 55:257-275.
Bacteriophage Therapy
Alexander Sulakvelidze,,* Zemphira Alavidze,and J. Glenn Morris, Jr.
Antimicrob Agents Chemother. 2001 Mar; 45(3): 649–659.
doi: 10.1128/AAC.45.3.649-659.2001
33. Literature
Samsygina, G. A., and E. G. Boni. 1984. Bacteriophages and phage therapy in pediatric
practice. Pediatria 4:67–70.
Schless, R. A. 1932. Staphylococcus aureus meningitis: treatment with specific
bacteriophage. Am. J. Dis. Child. 44:813–822
34. Literature
Peremitina, L. D., E. A. Berillo, and A. G. Khvoles. 1981. Experience in the therapeutic
use of bacteriophage preparations in suppurative surgical infections. Zh. Mikrobiol.
Epidemiol. Immunobiol. 9:109–110
Chopra, I., J. Hodgson, B. Metcalf, and G. Poste. 1997. The search for antimicrobial
agents effective against bacteria resistant to multiple antibiotics. Antimicrob. Agents
Chemother. 41:497–503.
Eaton, M. D., and S. Bayne-Jones. 1934. Bacteriophage therapy. Review of the
principles and results of the use of bacteriophage in the treatment of infections.
JAMA 23:1769–1939
35. Literature
Schless, R. A. 1932. Staphylococcus aureus meningitis: treatment with specific
bacteriophage. Am. J. Dis. Child. 44:813–822
Slopek, S., A. Kucharewicz-Krukowska, B. Weber-Dabrowska, and M. Dabrowski. 1985.
Results of bacteriophage treatment of suppurative bacterial infections. VI. Analysis of
treatment of suppurative staphylococcal infections. Arch. Immunol. Ther. Exp.
33:261–273.
Smith, H. W., and M. B. Huggins. 1987. The control of experimental E. coli diarrhea in
calves by means of bacteriophage. J. Gen. Microbiol. 133:1111– 1126
36. Literature
Yao, J. D. C., and R. C. Moellering, Jr. 1995. Antimicrobial agents, p. 1474–1504. In P. R.
Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of
clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.
Zhukov-Verezhnikov, N. N., L. D. Peremitina, E. A. Berillo, V. P. Komissarov, V. M.
Bardymov, A. G. Khvoles, and L. B. Ugryumov. 1978. A study of the therapeutic effect
of bacteriophage agents in a complex treatment of suppurative surgical diseases. Sov.
Med. 12:64–66.