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Bacteriology midterms

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1. The document discusses the process of tissue processing for light and electron microscopy. This includes fixation, dehydration, clearing, infiltration, embedding, sectioning, staining, and mounting of tissue samples. 2. Key steps in electron microscopy are fixation with glutaraldehyde and osmium tetroxide, embedding in plastic resin, sectioning with an ultramicrotome, and staining with heavy metal stains for high resolution imaging of cell structures. 3. Immunohistochemistry uses antigen-antibody reactions to identify and localize specific molecules in tissue. Primary antibodies attach to antigens and secondary antibodies tagged with markers like HRP or fluorescent dyes are used for visualization.

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Tissue Processing ,[object Object]
I. Light Microscopy (Histology) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],1-2
I. Light Microscope ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
I. Light Microscope ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
II. Fixation: preservation of tissue structure ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
II. Fixation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
II. Fixation ,[object Object],[object Object],[object Object],[object Object],[object Object]
III. Dehydration ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
III. Dehydration ,[object Object],[object Object],[object Object],[object Object],[object Object]
IV. Clearing ,[object Object],[object Object],[object Object]
V. Infiltration ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
VI. Embedding ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
VI. Embedding ,[object Object],[object Object],[object Object],[object Object],[object Object]
VII. Sectioning – Trimming the Block ,[object Object],[object Object]
VII. Sectioning ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],1-1
VII. Sectioning ,[object Object],[object Object],[object Object],[object Object]
VII. Sectioning ,[object Object]
VIII. Mounting sections ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
IX. Staining ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
IX. Staining ,[object Object]
IX. Staining ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
X. Coverslipping ,[object Object],[object Object],[object Object],[object Object]
XI. Pitfalls ,[object Object],[object Object],[object Object],[object Object],[object Object]
XI. Pitfalls ,[object Object],[object Object],[object Object]
XI. Pitfalls ,[object Object],[object Object],[object Object]
XI. Pitfalls ,[object Object],[object Object],[object Object],[object Object]
XI. Pitfalls ,[object Object],[object Object]
XI. Pitfalls ,[object Object],[object Object],[object Object]
XII. Interpretation ,[object Object],[object Object],1-30
Read Chapters 2-3 The Cytoplasm & The Cell Nucleus ,[object Object],[object Object]
Electron Microscopy & Immunohistochemistry (IHC) ,[object Object]
I. Electron Microscope ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],1-9
I. Electron Microscope ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],1-9
I. Electron Microscope 1-8
II. EM Fixation ,[object Object],[object Object]
III. EM Embedding & Sectioning & Staining ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
III. EM Viewing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],x500 x9000 1-11
I. Immunohistochemistry (IHC) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],1-25
II. Direct Labeling of antibodies ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
IV. Indirect Immunohistochemistry ,[object Object],1-24
IV. Indirect IHC ,[object Object]

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Bacteriology midterms

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