transduction is a process which that bacteriophage is transfer the genetic material to one to another bacterial cell .the transduction is have a two types that is generalized and specialized transduction .the two types of phage will be involve in the transduction process that is virulant and temptate pahge
transformation in bacteria is a classical example of horizontal gene transfer which leads to enhanced survivability and also introduction of variations that may lead to evolution
Autonomously replicating circular fragment present in DNA is called plasmids.
The term plasmid was first introduced by American molecular biologist Joshua Lederberg in1952.
An episome is a plasmid capable of inserting DNA into the host chromosome.
Because of their ability to transfer DNA from one bacterium to another, plasmids are extensively used in recombinant DNA technology or genetic engineering.
transduction is a process which that bacteriophage is transfer the genetic material to one to another bacterial cell .the transduction is have a two types that is generalized and specialized transduction .the two types of phage will be involve in the transduction process that is virulant and temptate pahge
transformation in bacteria is a classical example of horizontal gene transfer which leads to enhanced survivability and also introduction of variations that may lead to evolution
Autonomously replicating circular fragment present in DNA is called plasmids.
The term plasmid was first introduced by American molecular biologist Joshua Lederberg in1952.
An episome is a plasmid capable of inserting DNA into the host chromosome.
Because of their ability to transfer DNA from one bacterium to another, plasmids are extensively used in recombinant DNA technology or genetic engineering.
transduction is a mode of horizontal gene transfer in which the recipient does not come in contact with the donor bacterial cell, it is mediated by temperate phages.
BACTERIAL RECOMBINATION,PLASMIDS AND EPISOMESsushma93
Genetic recombination - transfer of DNA from one organism (donor) to another organism (recipient). The transferred donor DNA may then be integrated into the recipient's genetic material by various mechanisms
Bacterial recombination occurs in three ways
Transformation
Transduction
Conjugation
It is defined simply as a technique to efficiently and stably introduce foreign genes into the genome of target cells.
The insertion of unrelated, therapeutic genetic information in the form of DNA into target cells
BACTERIAL TRANSDUCTION
PRESENTED BY SAIRA FATIMA
ROLL NO:31
MSc 4 [2018-2020]
Department of MicroBiology & Molecular Genetics
University of the Punjab
Lahore, Pakistan
molecular biology phage vector, full lifecycle and all necessary information regarding lambda phage, it contain 2 types that is insertion and replacement.
transduction is a mode of horizontal gene transfer in which the recipient does not come in contact with the donor bacterial cell, it is mediated by temperate phages.
BACTERIAL RECOMBINATION,PLASMIDS AND EPISOMESsushma93
Genetic recombination - transfer of DNA from one organism (donor) to another organism (recipient). The transferred donor DNA may then be integrated into the recipient's genetic material by various mechanisms
Bacterial recombination occurs in three ways
Transformation
Transduction
Conjugation
It is defined simply as a technique to efficiently and stably introduce foreign genes into the genome of target cells.
The insertion of unrelated, therapeutic genetic information in the form of DNA into target cells
BACTERIAL TRANSDUCTION
PRESENTED BY SAIRA FATIMA
ROLL NO:31
MSc 4 [2018-2020]
Department of MicroBiology & Molecular Genetics
University of the Punjab
Lahore, Pakistan
molecular biology phage vector, full lifecycle and all necessary information regarding lambda phage, it contain 2 types that is insertion and replacement.
Prokaryotes can exchange DNA with eukaryotes, although the mechanisms behind this process are not well understood. Suspected mechanisms include conjugation and endocytosis, such as when a eukaryotic cell engulfs a prokaryotic cell and gathers it into a special membrane-bound vesicle for degradation.
It is a microbiology topic based on transduction in bacteria, and there is a big role for bacteriophage as it also does it's lytic and lysogenic cycles. It is important on the view of health and medicine
Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer.
the horizontal gene transfer in bacteria is not only important for survival but has its evolutionary significance too. this presentation is a prelude to the three classical types of HGT in bacteria
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2. TRANSDUCTION
• It refers to the process
whereby foreign DNA is
introduced into another
cell via a viral vector.
or
• It is the process by which
DNA is transferred from
one bacterium to another
by a virus.
3. The experiments implicated a bacteriophage as the vector or
transducing agent.
• What is Bacteriophage?
• The virus that infect the bacteria
are known as bacteriophage.
Most bacteriophage, the virulent
phages, undergo a rapid lyric
growth cycle in their host cells.
They inject there nucleic acid,
usually DNA, into the bacterium,
where it replicates rapidly and
also directs synthesis of new
phage proteins.
4. • Transduction occurs by either the lytic or lysogenic cycles.
•In a lytic infection, the host cells fills with virions and bursts.
•The result is cell death.
•Lysogenic infections are also known as latent infections.
•The viral genome becomes incorporated into the host cell’s DNA.
•It can remain this way for an extended period.
•The host cell lives.
5. Types of transduction
• GENERALIZED TRANSDUCTION
If all fragments of bacterial DNA
(I.e from any region of the bacterial
chromosome) have a chance to enter a
transducing phage, the process is called
generalized transduction.
6. • SPECIALIZED TRANSDUCTION
Bacterial genes can also be
transduced by bacteriophage in another
process called specialized transduction in
which certain temperate phage strains can
transfer only a few restricted genes of the
bacterial chromosome.
7. GENERAL USES OF THE TERM AND APPLICATIONS
• More generally, transduction is the process by which genetic material e.g:
DNA or siRNA, is inserted into a cell by a virus.
• Common techniques in molecular biology are the use of viral
vectors(including bacteriophages).
• Some medical applications are:-
• It provide resistance to anti-biotic drugs.
• Helps in the correction of genetic diseases by direct modification of genetic
errors.
• It is a common tool used by molecular biologists to stably introduce a
foreign gene into a host cell’s genome.