The review article summarizes the applications of silver nanoparticles for diverse sectors. Over the decades, nanoparticles used as dignified metals such as silver exhibited distinctive characteristics basically correlated
to chemical, physical and biological property of counterparts having bulkiness. Numerous studies reported that Nanoparticles of about 100 nm diameter play a crucial role in widely spread industries due to unique properties including the dimension of small particle, high surface area and quantum confinement and they dispersed without agglomeration. Decade of discoveries clearly established that shape, size and distribution of Silver nanoparticles strongly affect the electromagnetic, optical and catalytic properties, which are often an assortment of changeable synthetic methods and reducing agents with stabilizers. Generation after generation the postulates come forth about properties of silver for the ancient Greeks cook from silver pots and the old adage ‘born with a silver spoon in his mouth’ thus show that eating with a silver spoon was wellknown
as uncontaminated. Impregnation of metals with silver nanoparticles is a practical way to exploit the microbe aggressive properties of silver at very low cost. The nanoparticles help in targeted delivery of drugs, enhancing bioavailability, sustaining drug or gene effect in target tissues, and enhancing the stability. Implementations of silver partials in medical science and biological science have been noticed from years ago; however alteration with nanotechnology is innovative potential. Over 23% of all nanotechnology based products, diagnostic and therapeutic applications implanted with silver nanoparticles (e.g. In arthritic disease and wound healing, etc.) and widely known for their antifungal, antibacterial, antiviral effect, employed in textile fabrics and added into cosmetic products as antiseptic to overcome skin problems. Thus, Silver
nanoparticles (AgNPs) have been urbanized as an advanced artifact in the field of nanotechnology.
Introduction
Nanoparticle characterization techniques
Electron Microscope
Scanning electron microscope
Transmission electron Microscope
X-ray powder diffraction
Nuclear Magnetic Resonance
Review on green synthesis of silver nanoparticles using plant extract. Various green materials are used for the synthesis of Ag. Several synthesis method main emphasis on green method.
Review paper on the applications and challenges of gold nanoparticles in medicine and dentistry.
Gold nanoparticles is a game-changer in delivering patient care. Its versatility can be put to use in diagnosis, imaging and treatment of various conditions. It relatively recent innovation although gold is a metal that has had a lot of meaning in human civilisation.With a lot of potential left unexplored one has to what and watch the miracles this breakthrough has in store for medical science.
Introduction
Nanoparticle characterization techniques
Electron Microscope
Scanning electron microscope
Transmission electron Microscope
X-ray powder diffraction
Nuclear Magnetic Resonance
Review on green synthesis of silver nanoparticles using plant extract. Various green materials are used for the synthesis of Ag. Several synthesis method main emphasis on green method.
Review paper on the applications and challenges of gold nanoparticles in medicine and dentistry.
Gold nanoparticles is a game-changer in delivering patient care. Its versatility can be put to use in diagnosis, imaging and treatment of various conditions. It relatively recent innovation although gold is a metal that has had a lot of meaning in human civilisation.With a lot of potential left unexplored one has to what and watch the miracles this breakthrough has in store for medical science.
It consists of introduction about nano world and how it is different from the macroscopic world and what are the reasons. it gives information about silver nanoparticles antimicrobial property and it is various application. it consists of synthesis, characterisation of silver nanoparticles.
Antibacterial agents are very important in the textile industry, water disinfection, medicine, and food packaging. Organic compounds used for disinfection have some disadvantages, including toxicity to the human body; therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles (NPs) is increasing. This review focuses on the Preparation and their potential with good antimicrobial activity of Ag-NPs and Se-NPs against biofilm forming S. aureus. Such improved antibacterial agents locally destroy bacteria, without being toxic to the surrounding tissue. We also provide an overview of opportunities and risks of using NPs as antibacterial agents. In particular, we discuss the role of Ag-NPs and Se-NPs materials. Several manufactured nanoparticlesparticles with one dimension less than 100 nm are increasingly used in consumer products. At nano size range, the properties of materials differ substantially from bulk materials of the same composition, mostly due to the increased specific surface area and reactivity, which may lead to increased bioavailability and toxicity. Thus, for the assessment of sustainability of nanotechnologies, methods of manufacturing Nanoparticles, properties have to be studied.
The formation of nanoparticle and physiochemical parameters such as pH, monomer concentration, ionic strength as well as surface charge, particle size and molecular weight are important for drug delivery. Further, these nanoparticles have the capability to reverse
multidrug resistance a major problem in chemotherapy. Well-established therapies commonly employed in cancer treatment include surgery, Chemotherapy, immunotherapy, and
radiotherapy. The silver nanoparticles might be involved in neutralizing these adhesive substances, thus preventing biofilm formation. Selenium is also one of essential trace elements in the human body and has great importance in nourishment and medicine. Medicaldiagnostic field also developed to use the selenium nanoparticles and also studies on the increase efficiency of glutathione peroxidase and thioredosin reductase.
It consists of introduction about nano world and how it is different from the macroscopic world and what are the reasons. it gives information about silver nanoparticles antimicrobial property and it is various application. it consists of synthesis, characterisation of silver nanoparticles.
Antibacterial agents are very important in the textile industry, water disinfection, medicine, and food packaging. Organic compounds used for disinfection have some disadvantages, including toxicity to the human body; therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles (NPs) is increasing. This review focuses on the Preparation and their potential with good antimicrobial activity of Ag-NPs and Se-NPs against biofilm forming S. aureus. Such improved antibacterial agents locally destroy bacteria, without being toxic to the surrounding tissue. We also provide an overview of opportunities and risks of using NPs as antibacterial agents. In particular, we discuss the role of Ag-NPs and Se-NPs materials. Several manufactured nanoparticlesparticles with one dimension less than 100 nm are increasingly used in consumer products. At nano size range, the properties of materials differ substantially from bulk materials of the same composition, mostly due to the increased specific surface area and reactivity, which may lead to increased bioavailability and toxicity. Thus, for the assessment of sustainability of nanotechnologies, methods of manufacturing Nanoparticles, properties have to be studied.
The formation of nanoparticle and physiochemical parameters such as pH, monomer concentration, ionic strength as well as surface charge, particle size and molecular weight are important for drug delivery. Further, these nanoparticles have the capability to reverse
multidrug resistance a major problem in chemotherapy. Well-established therapies commonly employed in cancer treatment include surgery, Chemotherapy, immunotherapy, and
radiotherapy. The silver nanoparticles might be involved in neutralizing these adhesive substances, thus preventing biofilm formation. Selenium is also one of essential trace elements in the human body and has great importance in nourishment and medicine. Medicaldiagnostic field also developed to use the selenium nanoparticles and also studies on the increase efficiency of glutathione peroxidase and thioredosin reductase.
Biogenic– Biosynthesis Metallic Nanoparticles (MNPs) for Pharmacological, Bio...Al Baha University
In future, the biogenic– biosynthesis MNPs have wide perspective synthesis in healthcare, sustainable and renewable energy and
other commercial products. MNPs produced by nanotechnology have received global attention due to their extensive applications in
the biomedical and physiochemical fields. Biomolecules present in live plants, plant extracts and microorganisms such as: bacteria,
fungi, seaweeds, actinomycetes, algae and microalgae can be used to reduce metal ions to MNPs in a single-step and green synthesis
process. Biological green synthesis of MNPs has been always beneficial, more economical, energy efficient and eco-friendly approach,
which is free of toxic contaminates as required in therapeutic applications. The biosynthesis reduction of metal ion to base metal is
quite rapid, readily conducted at room temperature, pressure and easily scaled up.
The reducing agents involved include the various water-soluble plant metabolites (e.g. alkaloids, phenolic compounds, terpenoids,
flavonoids, saponins, steroids, tannins and other nutritional compounds) and co-enzymes. The polysaccharides, proteins and lipids
present in the algal membranes act as capping agents and thus limit the use of non-biodegradable commercial surfactants, which are
difficult to remove after the synthesis of MNPs. Metallic nanoparticles viz. cobalt, copper, silver, gold, platinum, zirconium, palladium,
iron, cadmium and metal oxides such as titanium oxide, zinc oxide, magnetite, etc. have been the particular focus of green biosynthesis.
Here we review the methods of making MNPs using plants extracts and microorganisms. Methods of particle characterization,
biomedical and environmental applications of MNPs are reviewed. In the near future, the application of clean, non-toxic, and ecofriendly
nanostructured material will be possible in industry and biomedicine.
Application of Nanomaterials in Medicine: Drug delivery, Diagnostics and Ther...Premier Publishers
Feyman’s Nanotechnology has multiple applications in clinical research for diagnosis, as nanodrugs or medicine, drug delivery as therapeutics. It is an endeavor to present here, the many varieties of nanomaterials and their application in physiology and medicine. Nanoparticles such as silver, gold, copper, zinc, calcium, titanium, magnesium have shown antimicrobial activity. The nanoparticles become highly reactive due to their change in physicochemical properties i.e. high surface-area-to-volume ratio. Antimicrobial gold nanoparticles are used in drug and gene delivery systems. Light induced plasmonic heating of gold nanoparticles might be an excellent photothermal therapeutic approach against cancer cells, bacteria and parasites. Zinc oxide nanoparticles are antimicrobial, anticancer, anti-diabetic, and anti-inflammatory theranostic agents. They develop cytotoxicity to cancer cells by increased ROS formation; inducing cancer cell death via the apoptosis signaling pathway. They deliver cancer drug such as doxorubicin, paclitaxel, etc. Non-toxic titanium dioxide is used in human food, drugs, cosmetics and food contact materials. Cadmium nanoparticles in the form of Quantum Dots are semiconductor metalloid-crystal structures have the potential for cellular imaging, cancer detection and treatment, drug delivery, etc. Magnesium oxide nanoflakes have been developed as drug carriers. Carbon can be used as nanotube for drug delivery, diagnosis, and treatment of cancer due to their unique chemical, physical, and biological properties, nanoneedle shape, hollow monolithic structure, and ability to carry drugs on their outer layers. Exosomes are the new kind of nanomaterials (20-200 nm) present in blood, saliva, breast milk, and sperm. These nanovessicles/nanostructures are released from cells which carry biomolecular information (miRNA, mRNA, proteins) as exosomal cargo. Exosomes are used in theranostic applications.
Nanobiomaterials are very effective components for several biomedical and pharmaceutical studies. Among the metallic, organic, ceramic and polymeric nanomaterials, metallic nanomaterials have shown certain prominent biomedical applications. Enormous works have been done to synthesize, analyse and administer the metallic nanoparticles for various kinds of medical and therapeutic applications, during the last forty years. In these analyses, the prominent biomedical applications of ten metallic nanobiomaterials have been reviewed from various sources and works. It has been found that almost nine of them are used in a very wide spectrum of medical and theranostic applications.
Study of Biocidal Activity of Copper A Reviewijtsrd
Copper ions, either alone or in copper complexes, have been used to disinfect liquids, solids and human tissue for centuries. Today copper is used as a water purifier, algaecide, fungicide, nematocide, molluscicide as well as an anti bacterial and anti fouling agent. Copper also displays potent anti viral activity. We have explained i the biocidal properties of copper ii the possible mechanisms by which copper is toxic to microorganisms and iii the systems by which many microorganisms resist high concentrations of heavy metals, with an emphasis on copper. Health care associated infections HAIs are a global problem associated with significant morbidity and mortality. Controlling the spread of antimicrobial resistant bacteria is a major public health challenge, and antimicrobial resistance has become one of the most important global problems in current times. The antimicrobial effect of copper has been known for centuries, and ongoing research is being conducted on the use of copper coated hard and soft surfaces for reduction of microbial contamination and, subsequently, reduction of HAIs. Dr. Pragya Tank "Study of Biocidal Activity of Copper: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-5 , August 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50696.pdf Paper URL: https://www.ijtsrd.com/chemistry/other/50696/study-of-biocidal-activity-of-copper-a-review/dr-pragya-tank
The next years will prove the importance of greensynthesis methods for MNPs and MONPs production because they are not
only easy to execute, fast, and cheap but also less toxic and environmentally ecofriendly. Nanoparticle synthesis using microorganisms
and plants by green synthesis technology is biologically safe, cost-effective, and environment-friendly. Plants and microorganisms
have established the power to devour and accumulate inorganic metal ions from their neighboring niche. The biological entities are
known to synthesize nanoparticles bothextra and intracellularly. The capability of a living system to utilize its intrinsic organic
chemistry processes in remodeling inorganic metal ions into nanoparticles has opened up an undiscovered area of biochemical analysis.
Metal nanoparticles (MNPs) and metal oxidenanoparticles (MONPs) are used in numerous fields. The new nano-based entities are
being strongly generated and incorporated into everyday personal care products, cosmetics, medicines, drug delivery, and clothing
toimpact industrial and manufacturing sectors, which means that nanomaterials commercialization and nanoassisted device will
continuously grow. They can be prepared by many methods such as green synthesis and the conventional chemical synthesis methods.
The green synthesis of nanoparticles (NPs) using living cells is a promising and novelty tool in bionanotechnology. Chemical and
physical methods are used to synthesize NPs; however, biological methods are preferred due to its eco-friendly, clean, safe, cost
effective, easy, and effective sources for high productivity and purity. Greensynthesis includes infinite accession to produce MNPs and
MONPs with demanding properties. The structure–function relationships between nanomaterials and key information for life cycle
evaluation lead to the production of high execution nanoscale materials that are gentle and environmentally friendly. Majority of plants
have features as sustainable and renewable suppliers compared with microbes and enzymes, as they have the ability to pick up almost
75% of the light energy and transform it into chemical energy, contain chemicals like antioxidants and sugars, and play fundamental
roles in the manufacture of nanoparticles. Plants considered the main factory for the green synthesis of MNPs and MONPs, and until
now, different plant species have been used to study this, but the determined conditions should be taken into consideration to execute
this preparation.
Antifungal Effects of Fugacil’s Silver Nanoparticles (AgNPs) against Various ...PearlGarner
Agricultural production is reduced worldwide every year due to plant disease; therefore, millions of dollars have been invested in efforts to control these plant diseases.
role of nanotechnology for crop protection in horticultural cropsgirija kumari
includes contents related to introduction about nanotechnology, nano particles, applications in agriculture and horticulture, crop protection applications and case studies
A variety of Nano-biomaterials are synthesised, characterised and tested to find out their potentialities by global scientific communities, during the last three decades. Among those, nanostructured ceramics, cements and coatings are being considered for major use in orthopaedic, dental and other medical applications. The development of novel biocompatible ceramic materials with improved biomedical functions is at the forefront of health-related applications, all over the world. Understanding of the potential biomedical applications of ceramic nanomaterials will provide a major insight into the future developments. This study reviews and enlists the prominent potential biomedical applications of ceramic nanomaterials, like Calcium Phosphate (CaP), Tri-Calcium Phosphate (TCP), Hydroxy-Apatite(HAP), TCP+HAP, Si substituted HAP, Calcium Sulphate and Carbonate, Bioactive Glasses, Bioactive Glass Ceramics, Titania-Based Ceramics, Zirconia Ceramics, Alumina Ceramcis and Ceramic Polymer Composites.
Nanoparticles are small molecules with size ranging between 1-100nm. Basis of their classification is their properties shapes and size. These find usage in wide range of industries from agricultural, biomedical, environmental and food. There are numerous ways of producing these nanoparticles using chemicals and biological means. Use of various micro-organisms (biological process) is highly effective in producing high quality, toxin free and cost effective nanoparticles.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
Background: Cancer is a disease caused when cells divide uncontrollably and spread into the surrounding tissue. Changes to DNA cause cancer. It is one of the most common and largest killer diseases in the world. It usually affects physically, and the disease can alter one’s perspective on life and personality. Many treatment options are there to treat cancer. Among them, chemotherapy treatment may have more side effects like lethargy, esophagitis, nausea, vomiting, Fatigue, and insomnia, the most common problems among chemotherapy patients in India. Methods: A quasi-experimental study with a sample size of 60, out of which 30 subjects were in the experimental group and 30 were in the control group. A convenient sampling method was used to select the subjects. A structured questionnaire tool was used to collect the data. Result: The result of the study showed that, during pre-test in the study group, among 30 subjects 3(10%) had moderate Fatigue, 22(73.33%) had severe Fatigue, 5(16.67%) had worst Fatigue and 12(40%) had moderate insomnia, 18(60%) had severe insomnia and in control group among 30 subjects, 6(20%) had moderate Fatigue, 13(43.33%) had severe Fatigue, 11(36.67%) had a worst fatigue, and 14(46.67%) had moderate insomnia, 16(53.33%) had severe insomnia. With post-test, in experimental group, 14(46.67%) had no fatigue, 16(53.33%) had mild fatigue, 14(46.67%) had no insomnia, 16(53.33%) had mild insomnia, and in control group, 6(20%) had moderate fatigue, 13(43.33%) had extreme fatigue, 11(36.67%) had worst fatigue, and 14(46.67%) had moderate insomnia, 16(53.33%) had severe insomnia. Conclusion: The study concluded that clients who were receiving chemotherapy had fatigue and insomnia problems. The Warm water foot bath therapy is very effective in clients undergoing chemotherapy in reducing Fatigue and insomnia. A positive correlation between pre-test and post-test was found by using the Mann-Whitney test.
Key-words: Cancer, Chemotherapy, Foot bath, Health, Warm water
Background: Alcohol has long been a global social and medical issue. According to W.H.O report. Total 3.3 million people die from
alcohol abuse annually. Alcoholic liver disease (A.L.D.) ranges from steatosis to liver cirrhosis. Chronic heavy drinkers get hepatitis
or cirrhosis 15 20% of the time
Methods: This study was c onducted in the general medicine inpatient department at PGIMER & C . in Bhubaneswar, Odisha,
Indi a. All hospitali z ed patients with liver illness who had previously t aken alcohol were screened. Each patient's alcohol
consumption, including native alcoholic beverages, was recorded. To support the diagnosis, all standard and extra examination s
were carri ed out. The modified Kuppuswamy scale was used to determine s s ocioeconomic class.
Results: The study comprised 186 participants with a median age of 46. The gender ratio was 3:1, with 139 (74.7%) men. Urban
populations have a greater prevalence of alcohol ic liver disease (60.75%) than rural populations (39.24%). The l ower
s ocioeconomic c lass (50.53%) has the highest rate of alcoholic liver disease. Men drink more (>700 gm/wk) and married people
drink more. A woman who drinks 140 280 grams per week for 10 1 5 years is more likely to develop alcoholic liver disease than a
man who drinks >700 grams per week for 15 years.
Conclusions: In this study, we conclude d that the prevalence of A .L. about S .E. is of utmost importance in developing
population based st r ategies that effectively educate individuals on the need to modify their drinking habits. This is crucial to
mitigate the occurrence of alcohol consumption and its associated repercussions.
Key-words: Socioeconomic status, Hospitalized Patients, Alcoholic Liver Disease
Background: One of the most common disorders in this age group, abnormal uterine bleeding (AUB), is the primary cause of most gynaecological problems in adolescents. Unfortunately, epidemiological data on AUB in teenagers is scarce, especially in the Indian subcontinent. The PALM-COEIN classification, where PALM stands for structural reasons and COEIN for functional causes, was employed in this single-center prospective observational study to evaluate the relative contributions of several etiological factors in AUB. To comprehend the etiological, dermographic, and therapeutic factors affecting menorrhagia in patients going through adolescence. Methods: Enrollment for females with AUB between 10 and 19 occurred between January and December 2022. A thorough history, physical examination, and laboratory evaluation, which in every case comprised standard testing, hormone analysis, and abdominal and pelvic ultrasonography were used to determine the cause of AUB. MRIs and CT scans were performed when needed. Results: There were 190 patients enrolled in total. Functional factors comprised the predominant aetiology of AUB among adolescent females: Adenomyosis=01 (0.52%), Polyp=1 (0.52%). Coagulopathy=2 (1.05%), Leomyoma=01 (0.52%), Malignancy=1 (0.52%), and PALM=4 (2.11%). COEIN=186 (97.89%), ovulation disorder=175 (92.15%), endometrial=01 (0.52%), iatrogenic=6 (3.15%), non-specified=2 (1.05%), and iatrogenic=6 (3.15%). Conclusion: The most frequent cause of AUB in the adolescent population is ovulatory abnormalities. Even though they are extremely rare, structural factors must be ruled out. A helpful technique for evaluating patients with AUB systematically is the PALM-COEIN classification.
Key-words: PALM-COEIN, Leiomyoma, AUB, Polycystic ovarian syndrome, Hormonal therapy
Derived from the bacterium Proteus vulgaris , chondroitin ABC lyase is an enzyme that can be used in treating proteoglycans that
affect neural activity (communication, plasticity). Chondroitinase can be used for vision abnormalities and spinal injuries. The
biological activity of chondroitinase is due to its ability to act on chondroitin sulfate proteoglycans (CSPGs) which are required for
normal functioning. Th is study aim s to examine various types and routes of administration of Chondr oitina se e n zymes. There is an
increasing application of chondro itin sulfate proteoglycans in spinal cord injury, vit reous attachment, and the management of
various carcinogenic conditions. Research must be done to create an effective chondroitinase delivery mech anism so that the
pharmacological activity seen in vitro and in preclinical research may be applied in the clinic. More studies are required to widen
the application of chondroitinase in therapeutics. In this review, chondroitinase ABC, B, and C are all di scuss ed. T he routes of
administration like caudal or ros tral, intracerebroventricular, hydrogels, and intrath ecal have been detailed. The current review
article highlights the different medical uses for chondroitinase, drug delivery methods for the enzym e, and chondroitinase
dispersion across bacteria. In conclusion, this study can reduce the chance of edema by the intracerebroventric ular route.
However, it is not effective for people due to the gyrencephalic anatomy of brain
Key-words: Chondroitinase, Chondroitin, Chondroitin Sulfate Proteoglycans, Spinal Injuries, Ocular Abnormalities, Proteoglycans
Background: Maturing is a widespread peculiarity. Advanced age is not in itself a sickness however is an ordinary piece of human existence length. A guardian, like wise called a career, home wellbeing assistant or individual consideration assistant, is the individual answerable for furnishing their clients with day-to-day private consideration and help with exercises. Methods: Exploration approach: unmistakable methodology research plan: graphic study research plan. The setting of the review: provincial areas of Bagalkot region. Information assortment strategy: organized polls test. The example was chosen by an arbitrary inspecting procedure. The analyst arbitrarily chose Shirur town as a provincial setting and was chosen for enrolment of subjects. Results: The information score of guardians was 41.06%, with mean and SD of 12.32±3.925. These discoveries uncover those guardians had normal information for advanced-age medical conditions. The mentality score of guardians was 73.73%, with a mean and SD of 110.6±11.008. These discoveries uncovers that parental figures have concur capable demeanour in regards to the advanced age medical conditions. Conclusion: At last, a critical co-connection between the information and demeanour at 0.001 the discoveries uncovers that there is a moderate positive relationship between the information and disposition of the advanced age medical issues.
Key-words: Assess, Care Giver, Health Problems, Knowledge, Old Age
Background: Adolescent is one of the most rapid phases of human development. Anemia is a deficiency in the number of RBC in your body. RBC carry oxygen around your body using a particular protein called hemoglobin. Normal hemoglobin level in adolescent girls 13-15 g/dl. According to WHO, the hemoglobin level 10- 11.9 g/dl is considered mild anemia, 7-9 g/dl is considered moderate, and less than 7 g/dl is called severe anemia. Methods: The present study is pre-experimental among 60 adolescent girls, using a disproportional stratified random technique. One experimental group of clients was selected without randomization and no control group was used. The data was collected by using the structured close-ended knowledge questionnaire. The data was analyzed using descriptive and inferential statistics regarding mean, frequency distribution, percentage, paired table t-test and chi-square test. Results: The overall findings reveal that the post-test knowledge mean score 26.24% with SD±5.94, which was 72% of the total score was more when compared to the pre-test knowledge mean score 12.98 with SD 5.94, which was 36.83% of total score. The calculated t-value of 24.91 was much higher than the table t-value 1.96 for the hypothesis. Conclusion: The study provides that VATP on knowledge regarding the preparation and use of moringa juice in managing anemia among adolescent girls was the scientific, logical and cost-effective strategy.
Key-words: Adolescent girls, Knowledge, VATP, Effectiveness, Socio-demographic variables.
Background: The research demonstrates that water birth comports and loosens mothers actually and intellectually. The buoyance lessens body weight and permits free development and situating to the mother. Buoyance and warm water upgrade uterine withdrawal and better blood flow, which builds uterine muscles' oxygenation, diminishes the mother's torment and increases maternal oxygenation of the child. Submersion of water assists with decreasing circulatory strain and additionally gives security, which hinders uneasiness or dread. Methods: The current review pre-trial study with 50, 4th-year B.Sc. Nursing is chosen through basic arbitrary methods. One gathering pre-test without control bunch configuration was utilized. Information was gathered through a self-directed, organized, shut, finished information survey. Data was examined by involving distinct and inferential measurements concerning mean rate by conveyance, matched "t" test, and Chi-square test for affiliation. Results: The pre-test reveals that out of 50 BSc 4th-year nursing students, the highest pre-test (62%) of BSc 4th-year nursing students had poor knowledge. Overall, the post-test knowledge score (22.6±4.19), 70.62% of the total score, was more than the pre-test knowledge score (8.76±3.95), 23.3%. The effectiveness of the assisted teaching programme, in this area, the mean knowledge score was 13.84 with SD±0.24, which was 43.25% of the total score. Hence, it indicates that the video-assisted teaching program effectively enhanced the knowledge of BSc 4th-year nursing students. Conclusion: This study concluded that video-assisted teaching programmes on knowledge regarding waterbirth among B.Sc 4th year Nursing students was the scientific, logical and cost-effective strategy.
Key-words: Effectiveness, Fourth year B.Sc. Nursing students, Knowledge, VATP, Water birth
Background: Post-menopausal women experience many physical, emotional, and mental symptoms during the post-menopausal period, and reflexology has grown into a complex therapeutic modality and has a range of effects. Reflexology will help put hormones back into a normal state and act like a process of emotional cleansing, relieving stress and restoring harmony to the body and soul. Hence, foot reflexology seems to be effective in treating post-menopausal symptoms. Methods: In the present study, pre-experimental i.e. one group pretest-posttest design, was adopted. The study was conducted on 30 post-menopausal women to assess their knowledge regarding foot reflexology. Samples were selected by using a convenient sampling technique. Data was collected using a structured knowledge questionnaire and analyzed using descriptive and inferential statistics. Results: The mean percentage of the pre-test score was 28%, and the post-test score was 76.65%. The mean and the standard deviation of the pre-test score were 5.60±1.71, and the mean and the standard deviation of the post-test score were 15.33±1.15. The total mean and standard deviation are 9.73±2.07 by comparing the pre-test and post-test scores. Hence, it was found that there is a significant difference between pre-test and post-test knowledge scores of post-menopausal women regarding foot reflexology. No significant association was found between post-test knowledge scores and socio-demographic variables on foot reflexology. Conclusion: The study concluded that a planned teaching program on knowledge regarding foot reflexology for post-menopausal women was a scientific, logical, and cost-effective strategy to reduce post-menopausal symptoms.
Key-words: Effectiveness, Foot reflexology, Post-menopausal women, Planned teaching program, Socio-demographic variables
Background: A 51-year-old woman had left lower abdomen pain for 18 hours with nausea and vomiting. Prior CT scans suggested pelvic neoplasms. Our hospital's emergency CT showed an enlarged uterus with cystic shadows, right adnexal cysts, and stomach fluid. Physical examination revealed left lower abdomen discomfort. A gynaecological examination revealed a painful, firm pelvic mass of 151210 cm. Further diagnosis is underway. Method: The patient underwent emergency exploratory laparotomy, discovering a twisted, swollen left ovary with a 540° rotation, classified as a benign cyst. It was found that the patient had congenital upper vaginal atresia and bilateral initial uteri. Pain was reduced after surgery, thanks to symptomatic treatment. An abnormal karyotype of 46, XX,1qh+ was found during genetic testing. Result: Fallopian tubes, uterus, and vagina develop from the embryonic accessory mesonephric duct. MRKH syndrome is caused by bilateral accessory mesonephric duct dysplasia and disappearance of the uterus or vagina. MRKH has three types, with Type 1 lacking uterus or vagina. Due to ovarian cyst torsion, this Type 1 MRKH with double initial uterus and upper vaginal atresia needed left adnexa resection. Genetic testing showed a typical female karyotype. MRKH's complex aetiology incorporates chromosomal abnormalities, emphasizing early cytogenetic evaluation for personalized treatment and fertility assistance. Conclusion: Early cytogenetic testing for MRKH syndrome patients is crucial for determining the underlying cause and guiding personalized treatment plans to restore reproductive function and improve quality of life.
Key-words: Double primordial uterus; MRKH syndrome; Upper vaginal atresia; Torsion of left ovarian cyst pedicle
Background: Cell phones have advanced to the degree of becoming a necessary piece of individuals' lives. Cell phones are utilised for correspondence, diversion, efficiency, interpersonal interaction, and gaming. In addition to supplanting the conventional cells, cell phones have likewise supplanted personal computers and numerous other comparative gadgets. Individuals these days feel indistinguishable from their cell phones. In lined with the rising improvement of innovation and excessive utilisation of cell phones, one of the significant issues that scientists have noticed and are chipping away at is cell phone addiction. Methods: It was a graphic study directed among 100 nursing students aged 19-22 in B.V.V.S. Institute of Nursing Sciences Bagalkot. Information was gathered utilising a structured knowledge questionnaire to survey socio-demographic information. The Stanford Sleepiness Scale (Alertness Test) was utilised to evaluate the classroom alertness of the nursing students and the Cell phone Addiction Scale-Short Version (SAS-SV) was utilised to assess the cell addiction of the nursing students. Results: An association was found between the year of studying and the classroom alertness of students (χ2 =3.9102) p<0.05. There was a significant negative correlation between cell phone addiction and classroom alertness of the nursing students, p<0.05. The r-value obtained was 0.80. Thus, the correlation between the two factors is seen as statistically significant. Conclusion In the wake of acquiring the consequences of the current work the scientist s saw a negati ve relationship between cell
pho ne addiction and the class room alertness of the students.
Key-words: Addiction, Alertness, Cell phone, Classroom, Phone addiction
Background: Chemical changes occur in the epididymis when the testicular sperm grows. When sperm and seminal fluids mix during ejaculation, a substance called semen is formed. The cervical mucus of a fertilized egg screens out the best possible sperm. For infertility, Intra Cytoplasmic Sperm Injection (ICSI) can be necessary. Test sperm that are DNA efficient, normal, and motile using Swim Up. Sperm could be damaged by reactive oxygen species that are produced during centrifugation. All infertility treatments should take these factors into account. Methods: The in vitro fertilization (ICSI) procedure was administered to fifty male patients who were 35 years old or younger and tested positive for normozoospermia, asthenozoospermia, and oligozoospermia. After obtaining informed consent, a Swim-Up was performed using both the full semen and a washed pellet. With sperm obtained from both methods, six Metaphase-2 stages of oocytes (MII oocytes) were implanted in each patient. A Tri-gas Bench-top incubator was used to put each injected oocyte in its 37°C setting. Results: The study showed that the age differences were insignificant (p=0.722), but significant variations emerged in sperm concentration before processing (p=1.030) and after (p=1.064). Sperm morphology differences were evident before processing (p=0.004) and after (p=0.002). No significant differences were noted in the number of Day 3 cleavage stage embryos. Conclusion: The study concluded that there is no significant difference between the two techniques regarding sperm washing efficiency.
Key-words: Sperm preparation methods, Swim-up, Centrifugation, ICSI, Fertilization, Day 3 Embryo
Background: The third most common musculoskeletal symptom in orthopaedic clinical practice is a sore shoulder, which can cause significant morbidity. It has been reported that 7–27% of the general population has it, and 36–66% of overhead arm athletes have it. Pathophysiology includes functional, degenerative, and mechanical factors. Most shoulder pain is subacromial pain syndrome (SAPS), often known as ‘shoulder impingement syndrome’. Impingement hypothesis: shoulder joint structures mechanically clash. SAPS accounts for 36–48% of shoulder discomfort. Methods: This observational study was conducted in the Department of Orthopaedics, MKCG Medical College and Hospital, Berhampur, among Eastern Indian outpatients. The study included adult patients (ages 18–75) of both sexes who presented to MKCG Medical College and Hospital's OPD with shoulder pain from December 2020 to November 2022 and were diagnosed with Shoulder Impingement Syndrome (SIS). Thorough histories and clinical exams were done. The Department of Radiology, MKCG Medical College and Hospital, Berhampur, performed conventional shoulder MRIs on the selected participants. Results: Most cases and controls were Type-II (43.3%), followed by Type-I (28.3% and 30%, 29.2% of the total group). The study's least common acromial shape was type-IV, seen in 5% of cases and 10% of controls (7.5% of the sample). Fisher's exact test showed no significant connection between subacromial impingement and acromial shape (p=0.65). With a p-value of 0.045, cases had a significantly greater acromial width (8.12±2.16 mm) than controls (7.51±0.81 mm). Conclusion: Sub-acromial impingement was unrelated to acromion morphology. There was no correlation between acromial morphology and rotator cuff injuries.
Key-words: Shoulder Impingement Syndrome, Acromion Morphology, MRI
Impact of Acceptance and Mindfulness-Based Intervention as an Add-on Treatment for Skin Diseases-Acne, Eczema and Psoriasis
http://dx.doi.org/10.21276/SSR-IIJLS.2020.6.5.2
Seasonal Incidence and Varietal Response of Gram against Helicoverpa armigera (Hubner) at Talwandi Sabo, Punjab
http://dx.doi.org/10.21276/SSR-IIJLS.2020.6.4.3
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Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...
Applications of silver nanoparticles in diverse sectors
1. Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
REVIEW PAPER
Applications of Silver nanoparticles in diverse sectors
Poonam Verma1
*, Sanjiv Kumar Maheshwari2
1
Research Scholar, Department of Biotechnology, IFTM University, Moradabad, India
2
Professor, Institute of Bio Science and Technology, Shri Ramswaroop Memorial University, Lucknow-
Deva Road, India
Received 10 June 2018; revised 29 August 2018; accepted 16 September 2018; available online 18 September 2018
* Corresponding Author Email: poonam.phdbiotech@gmail.com
How to cite this article
Verma P, Maheshwari SK. Applications of Silver nanoparticles in diverse sectors. Int. J. Nano Dimens., 2019; 10 (1): 18-36.
Abstract
The review article summarizes the applications of silver nanoparticles for diverse sectors. Over the decades,
nanoparticles used as dignified metals such as silver exhibited distinctive characteristics basically correlated
to chemical, physical and biological property of counterparts having bulkiness. Numerous studies reported
that Nanoparticles of about 100 nm diameter play a crucial role in widely spread industries due to unique
properties including the dimension of small particle, high surface area and quantum confinement and they
dispersed without agglomeration. Decade of discoveries clearly established that shape, size and distribution
of Silver nanoparticles strongly affect the electromagnetic, optical and catalytic properties, which are often
an assortment of changeable synthetic methods and reducing agents with stabilizers. Generation after
generation the postulates come forth about properties of silver for the ancient Greeks cook from silver pots
and the old adage ‘born with a silver spoon in his mouth’ thus show that eating with a silver spoon was well-
known as uncontaminated. Impregnation of metals with silver nanoparticles is a practical way to exploit
the microbe aggressive properties of silver at very low cost. The nanoparticles help in targeted delivery of
drugs, enhancing bioavailability, sustaining drug or gene effect in target tissues, and enhancing the stability.
Implementations of silver partials in medical science and biological science have been noticed from years
ago; however alteration with nanotechnology is innovative potential. Over 23% of all nanotechnology based
products, diagnostic and therapeutic applications implanted with silver nanoparticles (e.g. In arthritic disease
and wound healing, etc.) and widely known for their antifungal, antibacterial, antiviral effect, employed
in textile fabrics and added into cosmetic products as antiseptic to overcome skin problems. Thus, Silver
nanoparticles (AgNPs) have been urbanized as an advanced artifact in the field of nanotechnology.
Keywords: Antimicrobial Activity, Biofilm Forming, Nanosilver, Silver Nanoparticles, Staphylococcus Aureus
This work is licensed under the Creative Commons Attribution 4.0 International License.
To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
INTRODUCTION
The inimitable properties of Silver nanoparticles
make them interesting for commercial and scientific
appliance as an antimicrobial, disinfectant,
biosensor materials, composite fibers, cryogenic
superconducting materials, cosmetic products, and
electronic components. Numerous physical and
chemical methods are used for synthesizing and
stabilizing silver nanoparticles [1, 2]. The highly
accepted chemical approaches were employed
to synthesis silver nanoparticles that include
chemical reduction, using a variety of inorganic
and organic reducing agents, physicochemical
reduction, electrochemical techniques, and
radiolysis. Recently, nanoparticles synthesis had
been the foremost fascinating scientific areas
of inquiry and there’s growing attention to
supply eco-friendly exploitation of nanoparticles
(green chemistry). Green chemistry approaches
comprise mixed-valence polyoxometalates, Tollens,
polysaccharides, irradiation and biological method
that have advantages over conventional methods
concerning chemical agents associated with
environmental toxicity. Antimicrobial property of
silver nanoparticles expresses the relevance to
the medical field for investigations and curative
applications and needed by humans in everyday
life [3]. The Silver nanoparticle replacing the weak
2. 19Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
antibacterial agents involve in water disinfection,
textile industry, medical and food packaging, etc.
They might also be involved in neutralizing these
adhesive substances, thus preventing biofilm
formation [4].
Presently, nanochemistry becomes a rising
directions of Nanoscience [5]. The nanometer-
size metallic particles show unique and
significantly changed physical, chemical and
biological properties comparison of macro-
scaled counterpart based on their high surface-
to-volume ratio. These nanoparticles exhibit size
and shape-dependent properties as catalysts
and sensing to optics, antibacterial activity and
data storage [6-9]. For instance, the antimicrobial
activity of diverse metal nanoparticles, including
silver colloids is closely associated with their size;
that is, the smaller the silver nuclei, the greater
and therefore the antibacterial activity. Moreover,
the catalytic activity of these nanoparticles is
also dependent on their size as well as their
structure, shape, size distribution, and chemical-
physical environment. To overcome the irregular
size distribution, specific management of form,
size, and size distribution is achieved by different
strategies of synthesis, reducing agents and
stabilizers [10-12]. The optical properties of a
metal-bearing nanoparticle principally based on
its surface plasmon resonance, where the
Plasmon is the collective oscillation of the free
electrons within the metal-bearing nanoparticle.
It is renowned that the Plasmon resonant peaks
and line widths are sensitive to the size and forms
of the nanoparticle, the metallic species and the
surrounding medium. For instance, nanoclusters
composed of 2-8 silver atoms might be best for
a replacement of optical knowledge storage.
Moreover, fluorescent emissions through
clusters, may exploit at biological labels and
electroluminescent displays [13-14]. Recently,
AgNPs become interesting for therapeutic
applications of cancer as metastatic tumor agents,
in diagnostics and in inquiring, thus the review
explore the mechanism of metastatic tumor
activity,therapeuticapproachesandthelimitations
of nanoparticles in cancer medical aid. The Silver
nanoparticles are also effective against a broad
spectrum of gram-negative and gram-positive
bacteria, together with some antibiotic-resistant
strains [15]. The cluster of gram-negative bacteria,
against which the biocide activity of silver
nanoparticles has been confirmed, includes:
Acinetobacter [16], Escherichia [17], Pseudomonas
[18] and Salmonella [19]. The effective action of
silver nanoparticles was also reportable against
gram-positive bacteria: Bacillus [20], Enterococcus
[21], Listeria [22], Staphylococcus [23] and
Streptococcus [24]. Recent studies have shown
that the use of silver nanoparticles in combination
with certain antibiotics such as penicillin G,
amoxicillin, erythromycin, clindamycin and
vancomycin, creates a synergy effect against E. coli
and S. aureus [25]. Few studies had also shown
that silver nanoparticles may also be an efficient
weapon against viruses [26] thus inhibiting their
replication. Their activity has been confirmed even
against the HIV-1 [27] and influenza virus [28]. The
potency of processes resulting in the destruction
of viruses strictly depends on the shape and size
of nanoparticles [27]. The Silver nanoparticles
are effective and fast-acting agent that destroys
differing types of fungi like Aspergillus [29],
Candida [30] and Saccharomyces [15].
It focused on the overview on the Advantages
of silver nanoparticles. The aim of this review is,
therefore, to reflect on the advantages of silver
nanoparticles and future prospects, especially
the potential applications of the nanoparticles for
pharmaceutical industries. Moreover, we explore
the applications of silver nanoparticles in all
respective sectors and their mechanistic aspects
of silver nanoparticles as an antimicrobial agent.
Applications of AgNPs in various sectors
AgNPs are broadly applicable as anti-bacterial
agents for the food storage, health industry,
textile coatings and a variety of environmental
applications. The Products prepared by or
from AgNPs have been permitted by a range
of accredited bodies, including the US FDA, US
EPA, SIAA of Japan, Korea’s Testing and Research
Institute for Chemical Industry and FITI Testing
and Research Institute [31-36]. As anti-bacterial
agents, AgNPs were implemented in numerous
applications ranged involving disinfecting medical
devices, home appliances and water treatment
[37-38]. Moreover, this inspired the textile
industry to use AgNPs in numerous textile goods
(Table 1 and Fig. 1).
Significance of AgNPs Biomedical science
Owing the exceptional properties, AgNPs have
been used extensively in household implements,
the health care industry, for food storage,
3. 20
P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
environmental and biomedical applications.
Several reviews and book chapters have been
dedicated to the assorted areas for application of
AgNPs. Here, we explore the applications of AgNPs
in numerous biological and medical science field,
i.e medication, antifungal, antiviral, medicine,
anti-cancer, and anti-angiogenic. Here, we utterly
addressed previously-published seminal papers
and conclude with current information and
outcomes. A schematic diagram representing
various applications of AgNPs is providing in Fig. 2.
Antibacterial accomplishment- AgNPs emerge as
a substitute of antibacterial agent and have the
ability to overcome the bacterial resistance against
antibiotics. Therefore, it is necessary to widen
the use of AgNPs as antibacterial agents. Amid
various promising nanomaterials, AgNPs appear
as a potential medication negotiator because
of their massive surface-to-volume ratios and
crystallographic surface structure. The seminal
paper account by Sondi and Salopek-Sondi [39]
verified the antimicrobial activity of AgNPs against
Table 1: Applications of silver nanoparticles in different-different sectors.
Table 1: Applications of silver nanoparticles in different-different sectors.
S. No Fields Application of AgNPs
1 Biomedical application
Antibacterial accomplishment
Antifungal accomplishment
Antiviral accomplishment
Anti-inflammatory accomplishment
Anti-angiogenic activity
Anticancer exploit
2 Texiles application
UV rays blocking textile
Medicinal Textiles and Devices
3 Food industry
Nanotechnology and food packaging
Food processing
4 Environmental treatment
Air disinfection
Water disinfection
Drinking water disinfection
Groundwater and biological wastewater disinfection
5
Pharmacological
Applications
Antimicrobial activity
Larvicidal Activity
Wound Healing property
6 Miscellaneous
Water treatment
Catalytic activity
Fig. 1: Applications of silver nanoparticles in diverse sectors.
Fig. 1: Applications of silver nanoparticles in diverse sectors.
4. 21Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
Escherichia coli when E. coli cells treated with
AgNPs showed the accretion of AgNPs into the cell
wall and the formation of “pits” into the bacterial
cell walls, and ultimately leading to cell death.
In the same E. coli strain, smaller particles with
a larger surface-to-volume ratio showed more
efficient antibacterial activity than larger particles
[40]. Furthermore, the antibacterial activity of
AgNPs is not only the size, however also shape-
dependent [41]. AgNPs were synthesize by four
different types of saccharides with an average
size of 25 nm, showing high antimicrobial and
bactericidal activity against Gram-positive and
Gram-negative bacteria, counting highly multi-
resistant strains such as methicillin-resistant
Staphylococcus aureus. As predicted previously,
not only the size is important for determining
the efficiency, however also shape is important
for determining the efficiency, as AgNPs undergo
a shape-dependent interaction with the Gram-
negative organism E. coli [42]. Moreover, a detailed
study was carried out to investigate the efficiency
of the antimicrobial effects of AgNPs against yeast,
E. coli and Staphylococcus aureus and concluded
that, at low concentrations of AgNPs, the complete
inhibition of growth was observed in yeast and E.
coli, whereas a mild effect was observed for S.
aureus [43]. Biologically synthesized AgNPs from
the culture supernatants of Klebsiella pneumoniae
were evaluated; the efficiencies of various
antibiotics, such as penicillin G, amoxicillin,
erythromycin, clindamycin, and vancomycin
against Staphylococcus aureus and E. coli was
increased with AgNPs [25]. On comparing with
AgNPs, hydrogel-silver nanocomposites express
excellent antibacterial activity against E. coli.
One-pot synthesis of chitosan-Ag-nanoparticle
composite was found to have higher antimicrobial
activity than its components at their respective
concentrations, because one-pot synthesis
favors the formation of small AgNPs, attached
to the polymer, that can be dispersed in media
of pH≤6.3 [44]. Biologically produced AgNPs by
means of culture supernatants of Staphylococcus
aureus, illustrate significant antimicrobial activity
against methicillin-resistant S. aureus, followed by
methicillin-resistant Staphylococcus epidermidis
(MRSE) and Streptococcus pyogenes, while only
moderate antimicrobial activity was observed
against Klebsiella pneumoniae and Salmonella
typhi [45]. The mechanism of AgNP-induced cell
death was observed in E. coli through the leakage
of reducing sugars and proteins. Additionally,
AgNPs have knack to demolish the permeability
of the bacterial membranes via generating the
pits and gaps, signifying that AgNPs could damage
the structure of the bacterial cell membrane
[17]. Silver nanocrystalline chlorhexidine (AgCHX)
complex showed strong antibacterial activity
against the tested Gram-positive/negative and
methicillin-resistant Staphylococcus aureus
(MRSA) strains. Fascinatingly, the minimal
inhibitory concentrations (MICs) of nanocrystalline
Ag (III) CHX were much lower than the ligand
(CHX), AgNO3
, the gold standard and the silver
sulfadiazine [46]. Biofilms are not only leads to
antimicrobial resistance, but are involved in the
development of ocular-related infectious diseases,
such as microbial keratitis [47]. Kalishwaralal and
co-workers demonstrated the potential anti-
biofilmactivity against Pseudomonas aeruginosa
and Staphylococcus epidermidis. Similarly, guava
leafextractreducedAgNPs(Gr-Ag-NPs)beevidence
for significant antibacterial activity and stability
Fig. 2: Various Biomedical applications of AgNPs.
Fig. 2: Various biomedical applications of AgNPs.
5. 22
P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
against E. coli compared to chemically synthesized
AgNPs; the reason for this higher activity could
be the adsorption of biomolecules on the surface
of the Gr-Ag-NPs [48]. AgNPs synthesized by
Cryphonectria sp. prove antibacterial activity
against numerous infective microorganism,
as well as E. coli, S. aureus, Candida albicans,
and Salmonella typhi. Interestingly, the AgNPs
exhibited higher antibacterial activity against both
S. aureus and E. coli other than S. typhi and C.
albicans.
The AgNPs exhibit the prospective for anti-
biofilm activity aligned with “Pseudomonas
aeruginosa” and “Staphylococcus epidermidis”.
Similarly, guava leaf extract reduced AgNPs
(Gr-Ag-NPs) showed significant antibacterial
activity and stability against E. coli in contrast
to chemically synthesized AgNPs; the reason
might be due to higher activity for adsorption
of biomolecules on the surface of the Gr-Ag-
NPs [48]. AgNPs synthesized by Cryphonectria
sp. explain antibacterial activity against diverse
human pathogenic bacteria, counting S. aureus,
E. coli, Salmonella typhi, and Candida albicans.
Interestingly, these particular AgNPs exhibited
higher antibacterial activity against both S. aureus
and E. coli than S. typhi and C. albicans.
Antifungal accomplishment- Fungal infections
are more frequent in patients with immuno-
suppression and overcoming fungi-mediated
diseases is a tedious process, because currently
there is a limited number of antifungal drug
[30]. Consequently, there is a foreseeable and
insistent requirement to develop antifungal agent
that should be biocompatible, non-toxic, and
environmental friendly. To overcome the problem,
AgNPs play an important role as anti-fungal agents
against various diseases caused by fungi. Nano-
Ag illustrates effective anti-fungal activity beside
clinical isolate and “ATCC” strains of “Trichophyton
mentagrophytes” and “Candida” species with
concentrations of 1-7 μg/mL. Esteban-Tejeda
et al. [49] developed an inert matrix containing
AgNPs with a medium size of 20 nm into a soda-
lime glass that increased the biocidal activity.
Monodisperse Nano-Ag sepiolite fibers express
significant antifungal activity against Issatchenkia
orientalis. AgNPs exhibited excellent antifungal
activity against Aspergillus niger with MIC of 25
μg/ml against Candida albicans [50]. Bio-synthetic
AgNPs express superior antifungal activity with
“fluconazole” against “Phoma glomerata”,
“Phoma herbarum”, “Fusarium semitectum”,
“Trichoderma sp.”, and Candida albicans [51].
AgNPs stabilized by sodium dodecyl sulfate
showed enhanced antifungal activity against
Candida albicans compared to conventional
antifungal agents [52]. The antifungal activities
depend on size of different AgNPs were execute
beside mature “Candida albicans” and “Candida
glabrata” biofilms. Biologically synthesized AgNPs
exhibited antifungal activity against numerous
phytopathogenic fungi, as well as Alternaria
alternata, Sclerotinia sclerotiorum, Macrophomina
phaseolina, Rhizoctonia solani, Botrytis cinerea,
and Curvularia lunata at the concentration of
fifteenmg[53-54].Similarly,TheAgNPssynthesized
by Bacillus species exhibited strong antifungal
activity against the plant pathogenic fungus
Fusarium oxysporum at the concentration of 8 g/
mL [55]. The antifungal effectiveness of AgNPs
was estimated on combining with “nystatin” (NYT)
or “chlorhexidine” (CHX) against Candida albicans
and Candida glabrata biofilms. The results from
this investigation suggest that AgNPs combined
with either nystatin (NYT) or chlorhexidine
digluconate (CHG) showed better synergistic anti-
biofilm activity; however, the activity depends
on the species and drug concentrations [56]. The
bio-synthetic AgNPs reveal burly antifungal activity
adjacent to “Bipolaris sorokiniana” by the inhibition
of conidial germination [57]. Interestingly, AgNPs
not only inhibit human and plant pathogenic fungi,
but also effective against indoor fungal species
such as Penicillium brevicompactum, Aspergillus
fumigatus, Cladosporium cladosporoides,
Chaetomium globosum, Stachybotrys chartarum,
and Mortierella alpine cultured on agar media [58].
Antiviral accomplishment- Viral mediated diseases
are common and becoming more prominent
worldwide; therefore, developing anti-viral
agents is essential. The mechanisms of the
antiviral activity of AgNPs are an important
aspect in antiviral therapy. AgNPs have unique
interactions with bacteria and viruses based on
certain size ranges and shapes [27, 41, 59]. Due
to the antiviral activity, nano-Ag incorporated
into polysulfone ultrafiltration membranes; (nAg-
PSf) was evaluated against MS2 bacteriophage,
which shows the significant antiviral activity,
due to increased membrane hydrophilicity [60].
AgNPs have demonstrated efficient inhibitory
6. 23Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
activities against human immunodeficiency virus
(HIV) and hepatitis B virus (HBV) [61]. A study
focused towards the antiviral action of the AgNPs;
the information confirm that each scavenger cell
(M)-tropic and T-lymphocyte (T)-tropic strains
of HIV-1 were sensitive to the AgNP-coated
polyurethane condom (PUC) [62]. Even though,
numerous studies have revealed that AgNPs could
slow down the viability of viruses nevertheless
the exact mechanism of antiviral activity is still
obscure. The studies by Trefry and Wooley found
that AgNPs caused a four- to five-log reduction in
infective agent titre at concentrations that weren’t
cytotoxic to cells [63]. Interestingly, in the presence
of AgNPs, virus was capable of adsorbtion to cells,
and this viral entry is responsible for the antiviral
effects of AgNPs. The Hemagglutination assay
indicated that AgNPs might considerably inhibit
the growth of the influenza virus in Madin-Darby
canine urinary organ cells i.e. kidney. The study
as of intranasal AgNPs organization within mice
considerably improved survival, lower lung viral
titer levels, minor pathologic lesions in lung tissue,
and remarkable survival advantage after infection
with the H3
N2
influenza virus, suggesting that
AgNPs had a significant role in mice survival [64].
Biologically-synthesized AgNPs inhibited the
viability of herpes simplex virus (HSV) types 1 and
2 and human para-influenza virus type 3, based on
sizeandzetapotentialofAgNPs[65].Thetreatment
of Vero cells with “non-cytotoxic” concentrations
of AgNPs considerably repressed the replication
of “Peste des petits ruminant’s virus” (PPRV). The
mechanisms of viral replication are due to the
interaction of AgNPs with the virion surface and
the virion core [66]. Tannic acid synthesized the
assorted sizes AgNPs that is able to reduce “HSV-
2” infectivity in vitro and in vivo through direct
interaction,blockedvirusattachment,penetration,
and further spread [67]. The antiviral property of
Ag+ alone or combination with 50 ppb Ag+ and
20 ppm CO3
2-
(carbonate ions) was performed on
bacteriophage MS2 phage. The results from this
study evaluate that 50 ppb Ag+ alone was unable
to affect the phage, and the combination of 50
ppb Ag+ and 20 ppm CO3
was found to have an
effective antiviral property within a contact time
of 15 min [68].
Anti-Inflammatory accomplishment- Inflammation
is an early immunological response against
foreign particles by tissue, which is supported by
the enhanced production of pro-inflammatory
cytokines, the activation of the immune
system, and the release of prostaglandins and
chemotactic substances such as complement
factors, interleukin-1 (IL-1), TNF-α, and TGF-β
[69-72]. To defeat inflammatory action, we
should find efficient anti-inflammatory agents.
Among several anti-inflammatory agents, AgNPs
have recently played an important role in anti-
inflammatory field. AgNPs have been known
to be antimicrobial, but the anti-inflammatory
responses of AgNPs are still limited [73] reported
the anti-inflammatory activity in rat. The Rats
treated intra-colonically with 4 mg/kg or orally
with 40 mg/kg of nanocrystalline silver, showed
significantly reduced colonic inflammation. The
rata treated with AgNPs showed rapid healing
and improved cosmetic appearance, occurring
in a dose-dependent manner. Moreover, AgNPs
demonstrate major antimicrobial properties,
less wound inflammation, and modulation of
fibrogenic cytokines [74]. Continuing the previous
study, [70] investigated to gain further evidence
for the anti-inflammatory properties of AgNPs, in
which they used both in vivo and in vitro models
and found that AgNPs are able to down-regulate
thequantitiesofinflammatorymarkers,suggesting
that AgNPs could suppress inflammatory events in
the early phases of wound healing [70]. A porcine
contact dermatitis model showed that treatment
with nanosilver significantly increases apoptosis
in the inflammatory cells and decreased the levels
of pro-inflammatory cytokines [75]. Bio-synthetic
AgNPs declines the production of cytokines that
is induced by UV-B irradiation in HaCaT cells, and
also reduces the edema and cytokine levels in the
paw tissues [76].
Anti-AngiogenicActivity-Pathologicalangiogenesis
is a symbol of cancer and various ischemic and
inflammatory diseases [77]. There are several
research groups discovering novel pro- and anti-
angiogenic molecules to overcome angiogenic-
related diseases. Although there are several
synthetic molecules having anti-angiogenic
properties, the discovery of series of natural
pro- and anti-angiogenic factors suggests that
this may provide a more physiological approach
to treat both classes of angiogenesis-dependent
diseases in the future [78]. Recently, several
studies provided supporting evidence by both in
vitro and in vivo models showing that AgNPs have
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P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
both anti-angiogenic and anti-cancer properties.
Here, we summarize the important contribution
in cancer and other angiogenic related diseases
[79] confirmed the anti-angiogenic property of
biologically synthesize AgNPs using bovine retinal
endothelial cells (BRECs) as a model, where they
found inhibition of proliferation and migration in
BRECs after 24 h of treatment with AgNPs at 500
Nm concentration. The mechanisms of inhibition
of vascular endothelial growth factor (VEGF)
induced angiogenic process by the activation of
caspase-3 and DNA fragmentation; and AgNPs
inhibited the VEGF-induced PI3K/Akt pathway
in BRECs [80]. Followed by “Gurunathan et al.,”
concluded that the “anti-angiogenic” property
of AgNPs along with “pigment epithelium
derived factor” (PEDF) as a bench mark that is
known to as a potent anti-angiogenic agent [81].
Using BRECs as a model system, they evaluate
that AgNPs inhibited VEGF-induced angiogenic
assays. Moreover, they established that AgNPs
might obstruct the shape of new blood micro-
vessels by the inactivation of PI3K/Akt. They also
demonstrated the anticancer property of AgNPs
using various cytotoxicity assays in Dalton’s
lymphoma ascites (DLA) cells, and a tumor mouse
model showed significantly increased survival
time in the presence of AgNPs [82]. AgNPs
reduced with diaminopyridinyl (DAP)-derivatized
heparin (HP) polysaccharides (DAPHP) inhibited
basic fibroblast growth factor (FGF-2)-induced
angiogenesis compared to glucose conjugation
[80]. Kim et al. [83] developed an anti-angiogenic
Flt1 peptide conjugated to tetra-N-butyl
ammonium modified hyaluronate (HA-TBA),
and it was used to encapsulate genistein [83].
By means of human umbilical vein endothelial
cells (HUVECs), they found that genistein/Flt1
peptide-HA micelle inhibited the proliferation of
HUVECs, and the same reagents could drastically
reduce corneal neovascularization in silver
nitrate-cauterized corneas of Sprague Dawley
(SD) rats. The Ag2
S quantum dots (QDs) used as
nanoprobes to monitor lymphatic drainage and
vascular networks. The Ag2
S-based nanoprobes
showed long circulation time and high stability.
In addition, they were able to track angiogenesis
mediated by a tiny tumor (2-3 mm in diameter)
in vivo condition [84]. Recently, Achillea
biebersteinii flowers extract-mediated syntheses
of AgNPs with concentration of 200g/mL express
the 50% reduction in newly-formed vessels [85].
Anticancer exploit- worldwide, ever third person
has the possibility of cancer [86]. Although many
chemotherapeutic agents are currently being
used on different types of cancers however
the side effects are enormous and particularly
administrations of chemotherapeutic agents
by intravenous infusion are a tedious process
[86]. Therefore, it is indispensable to develop
technologies to avoid systemic side effects.
To overcome the issue, many researchers are
developing nanomaterials as an alternative tool
for formulations to target tumor cells specifically.
Gopinath et al. [87] investigated the molecular
mechanism of AgNPs and found that programmed
cell death was concentration-dependent under
conditions. Further, they observed a synergistic
effect on apoptosis using uracil phosphoribosyl
transferase (UPRT)-expressing cells and non-UPRT-
expressing cells in the presence of fluorouracil (5-
FU). Through the experimental conditions, they
observed that AgNPs not only induce apoptosis
but also sensitize cancer cells. The anticancer
property of starch-coated AgNPs was studied in
normal human lung fibroblast cells (IMR-90) and
human glioblastoma cells (U251). AgNPs induced
alterations in cell morphology, decreased cell
viability as well as metabolic activity and increased
oxidative stress leading to mitochondrial damage
and increased production of reactive oxygen
species (ROS), ending with DNA damage. Among
these two cell types, U251 cells showed more
sensitivity than IMR-90 [88]. The similar group also
confirmed the cellular uptake of AgNPs mainly
through endocytosis. AgNP-treated cells exhibited
various abnormalities, including upregulation of
metallothionein, downregulation of major actin
binding protein, filamin, and mitotic arrest [88].
The morphology of cancer cells suggests that
biologically synthesized AgNPs could induce cell
death very significantly [89] elegantly prepared
multifunctional silver-embedded magnetic
nanoparticles, in which the first type consisting of
silver-embedded magnetic NPs with a magnetic
core of average size 18 nm and another type
consist thick “silica shell” of silver with 16nm of
average size; the consequential silica-encapsulated
magnetic NPs (M-SERS dots) produce strong
surface-enhanced Raman scattering (SERS) signals
and have magnetic properties, and these two
significant properties were used for targeting
breast-cancer cells (SKBR3) and floating leukemia
cells (SP2/O). The “antineoplastic activities” of
8. 25Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
“protein-conjugated silver sulfide nano-crystals”
are dependent on size in human hepatocellular
carcinoma Bel-7402 and C6 glioma cells [90].
Instead of giving direct treatment of AgNPs into
the cells, some researchers developed chitosan
as a carrier molecule for the delivery of silver
to the cancer cells. For example, Sanpui et al.
demonstrated that “chitosan-based nanocarrier”
(NC) delivery of AgNPs induces apoptosis at
extremely low concentrations [91]. They then
examined cytotoxic efficiency using a battery
of biochemical assays. They found an increased
level of intracellular ROS in HT 29 cells. The Lower
concentrations of nanocarrier with AgNPs showed
better inhibitory results than AgNPs alone. Boca
et al. [92] reported that “chitosan-coated silver
nanotriangles” (Chit-AgNTs) point up growth in
cell mortality rate. In addition, human embryonic
cells (HEK) were able to take up Chit-AgNTs
efficiently, and the cytotoxic effect of various sizes
of AgNPs was significant in acute myeloid leukemia
(AML) cells [93]. A moment ago, the anticancer
assets of bacterial “B-AgNPs” and fungal extract-
produced AgNPs “F-AgNPs” was demonstrated
in human breast cancer MDA-MB-231 cells. Both
biologically produced AgNPs exhibited significant
cytotoxicity [94]. Among these, fungal extract-
derived AgNPs had strong effect than B-AgNPs,
owing to the type of reducing agents used for the
synthesis of AgNPs. Similarly, AgNPs derived from
Escherichia fergusoni showed dose-dependent
cytotoxicity against MCF-7 cells [94]. The synthesis
of AgNPs mediated by Plant extract validate
more prominent toxic effect againsed human lung
carcinoma cells (A549) than non-cancer cells like
human lung cells, indicating that AgNPs could
target cell-specific toxicity, which could be the
lower level of pH in the cancer cells [95]. Targeted
delivery is an essential process for the treatment
of cancer. To address this issue, Locatelli et al.
[96] developed multifunctional nanocomposites
containing “polymeric nanoparticles (PNPs)”,
alisertib (Ali) and AgNPs. PNPs conjugated with a
chlorotoxin (Ali@PNPs–Cltx) showed a nonlinear
dose-effect relationship, whereas the toxicity of
Ag/Ali@PNPs–Cltx remained stable. Bio-synthetic
AgNPs validate significant toxicity in “MCF7” and
“T47D” cancer cells by higher endocytic activity
than MCF10-A normal breast cell line [97]. Banti
and Hadjikakou explained the detailed account
of anti-proliferative and anti-tumor activity of
silver (I) compounds [98]. Bio-synthetic AgNPs are
capable to alter cancer cell morphology, which is
an early indicator for apoptosis. Apoptosis can be
determined by structural alterations in cells by
transmitted light microscopy.
Textile Applications- The Nano materials are now
commercially used in textile industries [99] by
incorporating into fiber or coated with fiber, for
instance Silver nanoparticles are used in T shirt,
sporting clothes, undergarments, socks etc [100].
UV rays blocking textile- “Inorganic UV blockers”
are more advantageous over “organic UV
blockers” as they are non-toxic and chemically
stable on exposing to both high temperatures
and UV [101-102]. Inorganic UV blockers are
generally the semiconductor oxides that are TiO2
,
ZnO, SiO2
and Al2
O3
. Among these semiconductor
oxides, titanium dioxide (TiO2
) and zinc oxide
(ZnO) are commonly used. It was resolute that
nano-sized “titanium dioxide” and “zinc oxide”
are comparatively more efficient in absorbing
and scattering UV radiation and provide better
protection against UV rays. This is due to the fact
that nano-particles have a larger surface area
per unit mass and volume than the conventional
materials, leading the increment ineffectively to
block UV radiation [101, 103]. Many research puts
forward for application of UV blocking treatment
to fabric using nanotechnology. UV blocking
treatment for cotton fabrics are developed by
means of sol-gel method. For this, thin layer of
titanium dioxide is formed on the surface of the
treated cotton fabric which provides excellent
UV protection; the effect can be maintained even
after 50 home launderings [102]. Apart from
titanium dioxide, zinc oxide nano rods of 10 to
50 nm in length are also applied to cotton fabric
to provide UV protection. Previous studies on UV
blocking effectively accomplished that the fabric
treated with zinc oxide nanorods were found to
have demonstrated an excellent UV protection
factor (UPF) rating [104]. This effect can be further
enhanced by means of various procedures for
Appling the nanoparticles on the fabric surface.
By applying padding process, nanoparticles, not
only coated onto the surface of the fabric, but
also penetrate into the interstices of the yarns and
the fabric, i.e. some portion of the nanoparticles
gets penetrated into the fabric structure. Such
Nanoparticles that does not stay on the surface
may not be very effective in shielding the UV rays.
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P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
It is worthwhile that only the right (face) side of
the fabric gets exposed to the rays and therefore,
this surface alone needs to be covered with the
nanoparticles for better UV protection. Spraying
(using compressed air and spray gun) the fabric
surface with the nanoparticles can be an alternate
method of applying the nanoparticles.
Medicinal Textiles and Devices- AgNPs synthesized
using A. dubius fabricated on the cotton cloth
and perspiration pad samples express high
resistance towards Corynebacterium, a sweat
microorganism [88]. The Antibacterial drug
activity of gauze fabric discs incorporated with
AgNPs, made from the inexperienced mature
thalli of Anthoceros exhibit antimicrobial activity
against Pseudomonas aeruginosa [89]. Curcuma
longa tuber dust enveloped silver nanoparticles
exhibited minimum bactericidal concentration
(MBC) for Escherichia coli BL-21 strain at 50mg/L.
The immobilization onto the fabric by means of
sterilewaterisreportedtospecifyhigherantiseptic
activity on compared with polyvinylidene fluoride
immobilized cloth [90]. The incorporation of
Azadirachta indica synthesized silver nanoparticles
into cotton cloth results in antibacterial drug effect
against E. coli [91].
Significance of AgNPs in Food Industry- AgNPs is
widely used in food industry reported by Cushen
and co-workers [105] chiefly due to antibacterial
activity and preservative free. Least amount
of AgNPs is safe for human however deadly to,
majority of viruses and bacteria, thus making them
useful for sanitization of food and water in day to
day lifestyle and an infection resistor in medicine.
Sunriver industrial Nano silver fresh food bag is
one of the commercially available bag in which
silver nanoparticles are applied [106]. AgNPs are
widely used in daily product that is soaps, food,
plastics, pastes and textiles due to their anti-
fungicidal and anti-bactericidal activities.
Nanotechnology and food packaging- A desirable
packagingmaterialshouldhavepermeabilitytogas
and moisture with strength and biodegradability
[107]. Nano-based “smart” and “active” food
packaging confers several advantages over
conventional packaging methods for providing
better packaging material with improved
mechanical strength, barrier properties and
antimicrobial films to nanosensing the pathogen
detection and alerting the consumers for the
safety status of food [108]. The nanocomposites
“an active material” for packaging and material
coatingcanalsobeusedtoimprovefoodpackaging
[109]. Many researchers build awareness towards
antimicrobial property [110-111] of organic
compounds i.e. essential oils, organic acids, and
bacteriocins [110-111] and their relevance in
polymeric matrices as antimicrobial packaging.
However, these compounds are not suitable for
various food processing steps that require high
temperatures and pressures because of high
sensitivity to the physical conditions. By using
inorganic nanoparticles, a strong antibacterial
activity can be achieved in low concentrations and
get more stable in extreme conditions. Therefore,
recently it becomes interesting for using these
nanoparticles in antimicrobial food packaging.
The “antimicrobial packaging” is active packaging
that contacts the food product or the headspace
inside which inhibits or retards the microbial
growth present on food surfaces [112]. Several
nanoparticles such as silver, copper, chitosan, and
metal oxide nanoparticles like titanium oxide or
zinc oxide have been reported to have antibacterial
property [113-114].
Foodprocessing-Somefoodprocessingtechniques
exploit enzymes to modify food components to
improve their flavor, nutritional quality or other
characteristics. The Nanoparticles used as a source
to immobilize these enzymes, which may aid in
the dispersion throughout the food matrices and
enhance their activity. The “Nano-silicon dioxide”
particles along with reactive aldehyde groups
that are covalently bound [115] to a porcine
triacylglycerol lipase effectively hydrolyze olive
oil. They facilitate the improvement in stability,
adaptability, and reusability [115]. Nanocharcoal
adsorbent is a nanoparticle product used for the
decoloration of food products [116].
Environmental treatments
Air disinfection- The Bioaerosols are airborne
particles of biological origins, including viruses,
bacteria, fungi, which are capable of causing
infectious, allergenic or toxigenic diseases.
Particularly, indoor air bioaerosols were found to
accumulate in large quantities on filters of heating,
ventilating, and air-conditioning (HVAC) systems
[117].Itisestablishedthatoutdoorairpollutionand
insufficient hygiene of an HVAC installation often
10. 27Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
resulted in lower quality of indoor air. Moreover,
the organic or inorganic materials deposited on
the filter medium after air filtration contribute
to microbial growth. The WHO estimated that
50% of the biological contamination present in
indoor air comes from air-handling systems, and
the formation of harmless micro-organisms such
as bacterial and fungal pathogens was found
in air filters. Most of these pathogens produce
mycotoxins, which aredangerous to human health,
thus microbial growth in air filters get reduced
by the integrating antimicrobial Ag-NPs into the
air filters. The antimicrobial effect of Ag-NPs on
bacterial contamination of activated carbon filters
(ACF) was studied by Yoon et al. [117]. The results
showed that Ag-deposited “ACF filters” were
effectively removes bioaerosols. The antibacterial
activityanalysisofAg-coated“ACFfilters”indicated
that two bacteria of “Bacillus subtilis” and E. coli
were completely inhibited within 10 and 60 min,
respectively. It was found that silver deposition did
not influence the physical properties of ACF filters
such as pressure drop and filtration efficiency,
however, the adsorptive efficacy was decreased
by silver deposition. Hence, the authors moreover
suggested that the quantity of Ag-NPs on the “ACF
filters” needs to get optimized to avoid excessive
reduction of their adsorptive characteristics and
to show effective antimicrobial activity. Recently,
Jung et al. [118] generated Ag-coated CNT hybrid
nanoparticles(Ag/CNTs)usingaerosolnebulization
and thermal evaporation/condensation processes
and considered their applicability to antimicrobial
air filtration. CNT and Ag-NPs aerosols mixed
together and attached to each other, forming Ag/
CNTs. The antimicrobial activity of Ag/CNT-coated
filters was tested against Gram-positive bacteria
S. epidermidis and Gram-negative E. coli. It was
found that when Ag/CNTs were deposited on the
surface of an air filter medium, the antimicrobial
activity against tested bacterial bioaerosols was
enhanced, compared with the deposition of CNTs
or Ag-NPs alone, whereas the filter pressure drop
and bioaerosol filtration efficiency were similar to
those of CNT deposition barely. It was reported
that the surface area of Ag-NPs was enhanced
by CNTs thus stands the main reason for the
higher antimicrobial filtration efficacy of Ag/CNTs
compared with that of pure Ag-NPs. Polymer air
filters made of polypropylene and silver nitrate
(AgNO3
) were examined for bacterial survival
[119]. The study demonstrates that the addition of
antibacterial AgNO3
agent to filters was effective
for preventing bacteria for colonizing filters. The
presence of an antimicrobial AgNO3
compound in
the air filters decreases the amount of bacteria,
which was observed in the case of both Gram-
negative and Gram-positive bacterial strains
of Micrococcus luteus, Micrococcus roseus, B.
subtilis, and Pseudomonas luteola. The apparent
reduction in bacterial cell growth on silver treated
filters made the technology of antimicrobial filter
treatment really necessary for the future.
Water disinfection
Drinking water disinfection- Water is one of
the most important substances on Earth and is
essential to all living things. About 70% of the
Earth is covered with water, but only 0.6% is
suitable for human consumption. Safe drinking
water is an important health and social issue in
many developing countries [120]. According to
the WHO, at least 1 billion people do not have
access to safe drinking water. Contamination of
drinking water and then the subsequent outbreak
of waterborne diseases are the leading cause of
deathinmanydevelopingnations[121].Moreover,
the spectrum and the incidence of some infectious
diseases are increasing worldwide, therefore,
there is an enormous need for treatments to
control the microbial contamination of water and
decrease the number of waterborne diseases.
Significant interest has arisen in using Ag-NPs
for water disinfection. The chemicals produced
nanosilver (chem-Ag-NPs) can be uniformly
decorated onto porous ceramic materials to
form an Ag-NPs–porous ceramic, composite by
using 3-aminopropyltriethoxysilane (APTES) as a
connecting molecule [122]. This composite can
be stored for long periods and is durable under
washing without loss of NPs. The sterilization
property of Ag-NPs-porous ceramic, composite
as an antibacterial water filter was tested with E.
coli. It was found that at a flow rate of 0.01 l min−1
,
the output count of E. coli was zero while input
water had a bacterial load of 105 CFU ml−1
. It also
confirms that the connection between the chem-
Ag-NPs and the ceramic based on the coordination
bonds between the -NH2 group at the top of
the APTES molecule and the silver atoms on
the surface of the NPs. This kind of connection
ensured that the chem-Ag-NPs were tightly fixed
to the interior channel walls of the porous ceramic
so that they can release sufficient quantity of silver
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P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
ions for antibiosis. Such Ag-NPs–porous ceramic
composites were successfully tested in drinking
water purification [123]. Additionally, the chem-
Ag-NPs can be coated on common polyurethane
(PU) foams by overnight exposure to chem. Ag-NPs
colloid [124]. The NPs are stable on the foam and
are not washed off with water and Morphology
of the foam gets retained after coating. The NPs
binding is due to its interaction with PU’s nitrogen
atom. On reaching flow rate of 0.5 l min−1
, after few
seconds the output count of E. coli was nil while
inputwaterhadabacterialloadof105CFUml-1
.The
“chem-Ag-NPs” were also successfully formed on
to the macroporous “methacrylic acid copolymer”
beads for disinfection of water [125]. This showed
that the chem-Ag-NPs formed on these copolymer
beads by the chemical reduction method were
stable underwater washing and their stability
was due to the interaction of the “chem-Ag-NPs”
to the “–COO−“ carboxylic functional group on
the “copolymer beads”. Polymeric microspheres
containing chem-Ag-NPs displayed highly effective
disinfection against two gram-negative bacteria (E.
coli, P. aeruginosa) and two gram-positive bacteria
(B. subtilis, S. aureus) strains. The chem-Ag-NPs
bound copolymer beads performed efficiently
in bringing down the bacteria count to zero for
all the tested strains. The bacterial adsorption
or adhesion analysis revealed that “copolymer
beads” containing “chem-Ag-NPs” do not have any
adsorption/adhesion of bacterial cell.
Groundwater and biological wastewater
disinfection- The impact of Ag-NPs on microbial
communities in wastewater treatment plants
was evaluated [126] and found that original
wastewater biofilms are highly tolerant to Ag-NP
treatment. With an application of 200 mg-1
Ag-NPs,
the reduction of biofilm bacteria measured by
heterotrophic plate counts was insignificant after
24 h. Biofilm can provide physical protection for
bacteria under Ag-NP treatment, and extracellular
polymeric substances (EPS) may play an important
role in this protection. Susceptibility to Ag-NPs is
different for every microorganism on the biofilm
microbial community. The study illustrates two
implications: (i) Ag-NPs could impact wastewater
biofilm microbial community structures,
depending on the characteristics of each strain,
e.g., its ability to produce EPS and growth rate
and the community interactions among these
strains; and Mpenyana-monyatsi et al. [127], (ii)
the effects of Ag-NPs on planktonic cells [127]
were different to those on wastewater biofilms.
Biofilm bacteria treated as isolated pure culture
is much more sensitive to Ag-NPs, in contrast to
the mixture of bacteria in the biofilm. A moment
ago, novel, cost-effective filter materials that was
coated with chem-Ag-NPs were developed for the
disinfection of ground water [127], thus revealed
that the chem-Ag-NPs were successfully deposited
on zeolite, sand, fibre-glass, anion and cation resin
substrates. The performance of these substrates
as an antibacterial water filter system was tested
for the removal of pathogenic bacteria of E. coli,
S. typhimurium, S. dysenteriae and V. cholera in
groundwater. The results exposed the maximum
bacteria exclusion efficiency of the Ag/cation resin
filter, with complete (100%) removal of all targeted
bacteria, and the lowest by the Ag/zeolite filter,
with an 8-67% removal rate.
Pharmacological Applications
Antimicrobial activity- Silver nanoparticles
synthesized using “Abutilon indicum” leaf [128]
extract exhibited highly potent antibacterial
activity agonized Staphylococcus aureus (16.8
mm), Bacillus subtilis (18.3 mm), Salmonella typhi
(14.5 mm), and Escherichia coli (17.2 mm) [128].
The impregnation of Ipomea carnea-AgNPs with a
cellulose acetate membrane, to form a structured
antimycobacterial membrane show 14mm zone
of inhibition on Mycobacterium smegmatis
[129]. “Boerhaavia diffusa” mediated AgNPs
express higher sensitivity against “Flavobacterium
branchiophilum” [130] than the other two fish
bacterial pathogens are Aeromonas hydrophila
and Pseudomonas fluorescens [130]. Lingo-berry
and cranberry juice assist AgNPs, found additional
against S. aureus, B. subtilis, and B. cereus and
fewer active against C. albicans and food borne B.
cereus [131]. The development of V. alginolyticus,
K. pneumoniae, P. aeruginosa, B. subtilis, and P.
shigelloides was extremely inhibited by the AgNPs
synthesized exploiting the inflorescence of the
Cocos nucifera. The “Antidermatophytic activity”
of AgNPs synthesized with lemon peel extract
showed zone of inhibition [132] at 12 ± 0.3SD,
11 ± 0.5SD against T. mentagrophytes and C.
albicans, respectively, however, no activity noticed
against T. rubrum [132]. Triangular, hexagonal,
and spherical AgNPs of 78nm and 98 nm in size,
formed by the leaf extracts of Caesalpinia coriaria
showed higher bactericide activity against E. coli
12. 29Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
(12 mm) and Pseudomonas aeruginosa (18 mm)
[133]. Apoptosis of C. albicans and S. cerevisiae by
P. oleracea-mediated AgNPs is due to generation
of reactive oxygen species and decreased
production of hydroxyl radicals initiated by the
phytoconstituents capped on the synthesized
AgNPs [134]. In vivo analysis of biochemical and
microscopic anatomy provides evidence regarding
bactericide impact of AgNPs synthesized using
Leucas aspera on fish models (Aeromonas
hydrophilaandCatlacatla)[135].TheAntimicrobial
activity of Sphaeranthus amaranthoides extract
synthesized silver nanoparticles Schlinkert et al.
[136] was reported to be amplified because of the
destabilization of the outer membrane, blocking
bacterial respiration, and depletion of intracellular
ATP leads to the denaturation of microorganism
cell wall. AgNPs synthesized using Vinca rosea
leaf extract showed [136] promising inhibition
against Staphylococcus aureus, Lactobacillus,
Escherichia coli, and Pseudomonas fluorescens
at 10 𝜇L concentrations [136]. Mukia scabrella
synthesized AgNPs showed 81.81%, 90%, and
63.23% antibacterial activity against nosocomial
Gram negative bacterial pathogens Pseudomonas
aeruginosa, Klebsiella pneumoniae, and
Acinetobacter, respectively [137]. The highest
zone of inhibition (16mm) was obtained for the
AgNPs synthesized using “Citrus sinensis” [40] and
Centella asiatica against Pseudomonas aeruginosa
compared to that of AgNPs produced using
Syzygium cumini and Solanum trilobatum [40].
Daturaalba(Nees)leafderivedsilvernanoparticles
showed better inhibitory zone (20 mm) against
Clostridium diphtheriaeand cell death is accounted
and accompanied by the protein denaturation and
rupturing of bacterial cell wall [43]. The AgNPs
synthesized using a extract of methanol from
“Solanum xanthocarpum” berry signify a stronger
“anti-H. pylori” [25] activity and a noncompetitive
inhibition were concluded from Lineweaver-Burk
plots [25]. Desmodium triflorum extract aided
AgNPs inhibited the growth of Staphylococcus
and E. coli by 62 and 88%, respectively, at 14–60
𝜇g/cm3 concentrations after 24 hours, while 100
𝜇g/cm3
concentration showed approximately
100% inhibition [44]. Gelidiella acerosa extract
synthesized AgNPs are highly active against tested
fungal species at a 50 𝜇L concentration against
Mucor indicus (22.3 mm) and Trichoderma reesei
(17.2 mm) compared to the standard antifungal
agentClotrimazole[45].Maximuminhibitoryzones
(25 and 27mm) were noted for Ocimumsanctum
leaf extract aided AgNPs against Proteus vulgaris
and Vibrio cholerae, respectively. The leaf
extract having nanoparticles of Vitex negundo
showed a minimum inhibition rate against the
aforementioned bacterial pathogens [46]. Silver
nanoparticles using leaf broth of Gliricidia sepium
at 50 𝜇L concentrations showed 3mm zone of
inhibition [47] for Staphylococcus aureus and 2mm
for Escherichia coli, Pseudomonas aeruginosa, and
Klebsiella pneumonia [47].
Larvicidal Activity- The larvicidal activity of Leucas
aspera aided synthesized AgNPs demonstrate
the maximum efficacy at LC50 values of 8.5632,
10.0361, 14.4689, 13.4579, 17.4108, and 27.4936
mg/L and LC90 values of 21.5685, 93.03928,
39.6485, 42.2029, 31.3009, and 53.2576mg/L,
[48] respectively, against the fourth instar larvae of
A. aegypti [48]. AgNPs synthesized with Drypetes
roxburghii(Wall.)express100%mortalityinsecond
instar larvae of Anopheles stephensi at 5 ppm
concentration and 100% mortality in all instars of
Culex quinquefasciatus and Anopheles stephensi,
respectively, at double the concentrations [138].
AgNPs of size 25-30 nm synthesized using an
aqueous leaf extract of Nerium oleander showed
highest mortality against both larvae and pupae
of Anopheles stephensi [139]. The exposure of the
larvae to assorted concentrations of Pedilanthus
tithymaloides-AgNPs [140] showed 100%
mortality from first to fourth instars and pupae of
A. aegypti after 24 h. Lethal concentration (LC50)
values of AgNPs were found to be 0.029, 0.027,
0.047, 0.086, and 0.018% against the larval and
pupal stages, with no mortality with “control”
[140]. Synthesized AgNPs using Sida acuta are
reported to have significant activity against the
vector mosquito’s A. stephensi, A. aegypti, and
C. quinquefasciatus, respectively [141]. The IC50
values for the “antiplasmodial activity” of the
AgNPs synthesized using “aqueous extracts” [142]
of Ashoka and Neem leaves against Plasmodium
falciparum are 8 and 30 𝜇g/ml respectively [142].
Vinca rosea synthesized AgNPs does not exhibit
any conspicuous toxicity on Poecilia reticulata
after 24, 48, and 72h of exposure, but are reported
to possess the potential to control A. stephensi
and C. quinquefasciatus [143]. “Euphorbia hirta”
synthesized AgNPs showed highest larval mortality
values of LC50 against larvae and pupae [144]. The
adulticidal and larvicidal activity of synthesized
13. 30
P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
AgNPs of C. quadrangularis represent 100%
mortality against H. maculate and R.(B.) microplus
[30]. The Appreciable larvicidal activity of
synthesized AgNPs by utilizing the aqueous extract
of Eclipta prostrata is reported against Anopheles
subpictus and Culex tritaeniorhynchus [49].
Wound healing property- Silver nanoparticles
synthesized within the network of peptide
fibers using ultraviolet irradiation repressed the
microorganism growth of E. coli, P. aeruginosa,
and S. aureus. AgNPs containing hydrogels on
HDFa cells didn’t show any vital influence on
cell viability [145]. “AgNPs hydrogel” derived
from “Arnebia nobilis” root extract examined for
wound healing action in an excision [69] animal
model exert a positive result attributed to their
antimicrobial potential and provided a novel
therapeutic direction for wound treatment in
clinical observe [69]. “Indigofera aspalathoides”
mediated AgNPs were eamined for wound-
healing applications following excision in animal
models [70]. AgNPs derived from Chrysanthemum
morifolium added to clinical ultrasound gel and
used on an ultrasound probe were found to
exhibit antiseptic activity contributive to the
sterility of the instrument [71]. In vitro study of
the AgNPs-based dressing, Acticoat Flex three
applied to a 3D embryonic cell culture [72] and
partial thickness burns patient showed that AgNPs
greatly reduce mitochondrial activity and cellular
staining techniques revealed nuclear integrity
with no signs of death [72]. AgNPs drive the
differentiation of fibroblasts into myofibroblasts
and promote wound contraction, thereby
increasing the wound healing effectiveness
[73]. The reduction in wound inflammation with
modulation in the liver and urinary organ functions
was observed throughout skin wound healing
by the positive effects of silver nanoparticles
through their antimicrobial properties [74].
AgNPs play a responsibility in dermal contraction
and epidermal reepithelialisation during wound
healing; contribute to the increased rate of wound
closure [75]. AgNPs prepared extracellularly
using the fungus Aspergillus niger are reported
to modulate cytokines involved in wound healing
within the excision rat model [76]. A considerable
reduction in wound-healing was observed
in a median time of 3.35 days for the AgNPs
incorporated onto the cotton cloth and dressings
and microorganism clearance was also improved
from infected wounds devoid of adverse effects
[77-80, 83, 85-87]. Silver nanoparticles exert
antimicrobial properties inflicting reduction in
wound inflammation and modulation of fibrogenic
cytokines [146].
Miscellaneous Applications
Manilkara zapota leaf extract mediates the
synthesis of AgNPs and showed a caricidal activity
atLC503.44mg/LagainstRhipicephalus(Boophilus)
microplus (Rajakumar and Rahuman, 2012) [147].
AgNPs synthesized using Jatropha gossypifolia
plant extract showed higher amoebicidal activity
against Acanthamoeba castellanii trophozoites
[148]. The nonlinear refraction and absorption
coefficient values of AgNPs synthesized using
Coriandrum sativum extract measured by Z-scan
technique with ns laser pulses showed superior
opticalnonlinearitycomparedtothosesynthesized
through other procedures [149].
Water treatment- Stable AgNPs synthesized using
Anacardium occidentale fresh leaf extract at 80o
C
bud as a novel probe for sensing chromiumions [Cr
(VI)] in tap water [150]. The population of bacteria
decreased once the concentration of silver
nanoparticles prepared using 10mg leaf extract
(Prosopis juliflora) was treated with 100mL of
waste material after 6 h and will increase because
the time of incubation increases [151].
Catalytic activity- The size dependent, catalytic
activity of the synthesized AgNPs using Kashayam,
Guggulutiktham, was established in the reduction
of Methylene Blue (MB) by NaBH4
[152]. Acacia
nilotica pod mediated silver nanoparticles
changed glassy carbon conductor express larger
catalytic activity on the reduction of benzyl
chloride compared to those of glassy carbon
and metallic Ag electrode [153]. Photocatalytic
degradation of methyl orange was measured
spectrophotometrically using Ulva lactuca and
synthesized AgNPs as nanocatalyst under visible
light illumination [154]. The manufactured
AgNPs using Gloriosa superba plant extract act
through the electron relay effect and influence
the degradation of methylene blue at the end
of the 30 min [155]. Hydrogen peroxide reduces
quickly with the outstanding catalytic activity of
polydispersed silver nanoparticles synthesized
via Triticum aestivum (khapali ghahu) extract
[156]. The reduction of 4-nitrophenol (4-NP) into
14. 31Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
4-aminophenol (4-AP) carried out with the Breynia
rhamnoides-AgNPs having NaBH4 and establish to
be depend on the nanoparticle size or the stem
extract concentration [157].
CONCLUSION
The review encompasses various applications
of the silver nanoparticles in numerous sectors.
New insights about the pharmacological
applications such as anticancer, larvicidal,
medical textiles, and devices are gleaned with
these exotic silver nanoparticles. Hence, these
biogenically synthesized silver nanoparticles
can lead a major payoff in the field of bio-
nanomedicine. Nanotechnology represents a
modern and innovative approach to develop
and test new formulations based on metallic
nanoparticles with antimicrobial properties. Silver
nanoparticles represent a prominent nanoproduct
with potential application in medicine and
hygiene. Characteristics of silver nanoparticles
such as shape and size are important not only for
augmenting the antimicrobial activity, but also
for reducing tissue and eukaryotic cell toxicities.
The possible risks to human health posed by
silver nanoparticles and increased entry into the
environment, with subsequent spread of microbial
resistance, are of increasing concern given the rise
of silver-containing products on the market.
FUTURE PROSPECTS
Itisimportanttonotethatdespiteofdecayuses,
the evidence of toxicity of silver is still not clear.
With observed advantages such as long-lasting
effect and enhanced bactericidal activity, the Ag-
NPs are promising for environmental treatments
contaminated with gastrointestinal bacteria and
other infectious pathogens. More significantly,
the powerful disinfectant activity of Ag-NPs can
open a brand new generation of silver-containing
disinfectionproductsforcontrollingandpreventing
further outbreak of diseases (e.g. biofilm, diarrhea,
and cholera). However, the emerging questions
on the disinfectant ability of Ag-NPs against viral
infections (e.g. Rota virus, A-H5N1 influenza virus,
Enterovirus 71, etc.) need more clarification.
Further analysis of disinfectant potency by silver-
based NPs for real environmental contaminations
are more demanding. Research in the field of
Ag-modified textile fibers will be conducted in
different directions: in-situ synthesis of AgNPs in
textile fibers with the use of environment friendly
chemicals, pretreatment of textile fibers to
increase the adsorption of AgNPs and to stabilize
their embedment in the fibers as well as to control
the release of AgNPs from the fiber surfaces,
improvement of the mode of Ag application to
preserve the washing fastness of the coating,
development of new application processes
to achieve multifunctional textile fibers with
antimicrobial properties, investigation regarding
impact of Ag-modified textile fibers on human
health and the environment.
ACKNOWLEDGMENTS
Authors are highly thankful to the Vice
Chancellor, IFTM University, Moradabad to provide
us the facility to proceed this research work.
CONFLICT OF INTEREST
The authors declare that there is no conflict of
interests regarding the publication of this review
article.
REFERENCES
1. Senapati S., (2005), Biosynthesis and immobilization of
nanoparticles and their applications. University of pune,
India.
2. Klaus-Joerger T., Joerger R., Olsson E., Granqvist C. G.,
(2001), Bacteria as workers in the living factory: Metal-
accumulating bacteria and their potential for materials
science. Trends in Biotechnology. 19: 15–20.
3. Verma P., Maheshwari S. K., (2017), Minimum biofilm
eradication concentration (MBEC) assay of Silver
and Selenium nanoparticles against biofilm forming
Staphylococcus aureus. JMSCR. 5: 20213-20222.
4. Verma P., (2015), A review on synthesis and their
antibacterial activity of Silver and Selenium nanoparticles
against biofilm forming Staphylococcus aureus. World J.
Pharm. Pharmaceut. Sci. 4: 652-677.
5. Sergeev G. B., Shabatina T. I., (2008), Cryochemistry of
nanometals. Colloids Surf. A: Physicochem. Eng. Aspects.
313-314: 18-22.
6. Sudrik S., Chaki N., Chavan V., Chavan S., Sonawane H.,
Vijayamohanan K., (2006), Silver nanocluster redox couple
promoted nonclassical electron transfer: An efficient
electrochemical Wolff rearrangement of α-Diazoketones.
Chem. Eur. J. 12: 859-864.
7. Choi Y., Ho N., Tung CH., (2007), Sensing phosphatase
activity by using gold nanoparticles. Angew Chem. Int. Ed.
46: 707-709.
8. Yoosaf K., Ipe K., Suresh C.H., Thoma K.G., (2007), In situ
synthesis of metal nanoparticles and selective Naked-Eye
detection of Lead ions from aqueous media. J. Phys. Chem.
C. 111: 12839-12847.
9. Vilchis-Nestor A., Sanchez-Mendieta V., Camacho-Lopez
M., Gomez-Espinosa R., Camacho-Lopez M., Arenas-
Alatorre J., (2008), Solventless synthesis and optical
properties of Au and Ag nanoparticles using Camellia
sinensis extract. Mater. Lett. 62: 3103-3105.
15. 32
P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
10. He B., Tan J., Liew K., Liu H., (2004), Synthesis of size
controlled Ag nanoparticles. J. Mol. Catal. A: Chem. 221:
121-126.
11. Chimentao R., Kirm I., Medina F., Rodrıguez X., Cesteros
Y., Salagre P., Sueira J., (2004), Different morphologies of
silver nanoparticles as catalysts for the selective oxidation
of styrene in the gas phase. Chem. Commun. 4: 846-847.
12. Zhang W., Qiao X., Chen J., Wang H., (2006), Preparation of
silver nanoparticles in water-in-oil AOT reverse micelles. J.
Colloid Interf. Sci. 302: 370-373.
13. Berciaud S., Cognet L., Tamarat P., Lounis B., (2005),
Observation of intrinsic size effects in the optical response
of individual gold nanoparticles. Nano Lett. 5: 515-518.
14. Kossyrev P. A., Yin A., Cloutier S. G., Cardimona D. A.,
Huang D., Alsing P., Xu J. M., (2005), Electric field tuning
of plasmonic response of nanodot array in liquid crystal
matrix. Nano Lett. 5: 1978-1981.
15. Wright J. B., Lam K., Hansen D., Burrell R. E., (1999), Efficacy
of topical silver against fungal burn wound pathogens. Am.
J. Infect. Control. 27: 344-350.
16. Niakan S., Niakan M., Hesaraki S., Nejadmoghaddam M.
R., Moradi M., Hanafiabdar M., (2013), Comparison the
antibacterial effects of nanosilver with 18 antibiotics on
multidrug resistance clinical isolates of Acinetobacter
baumannii. Jundish. J. Microbiol. 6: e8341.
17. Li W. R., Xie X. B., Shi Q. S., Zeng H. Y., Yang Y. S., Chen
Y. B., (2010), Antibacterial activity and mechanism of
silver nanoparticles on Escherichia coli. Appl. Microbiol.
Biotechnol. 85: 1115-1122.
18. Niakan M., Azimi H. R., Jafarian Z., Mohammad Taghi
G., Niakan S., Mostafavizade S. M., (2013), Evaluation of
nanosilver solution stability against Streptococcus mutans,
Staphylococcus aureus and Pseudomonas aeruginosa.
Jundishap. J. Microbiol., 6: e8570.
19. Petrus E. M., Tinakumari S., Chai L. C., Ubong A., Tunung R.,
Elexson N. N., (2011), A study on the minimum inhibitory
concentration and minimum bactericidal concentration of
nano colloidal silver on food-borne pathogens. Int. Food
Res. J. 18: 55-66.
20. Shahrokh S., Emtiazi G., (2009), Toxicity and unusual
biological behavior of nanosilver on Gram-positive and
negative bacteria assayed by Microtiter-Plate. Eur. J. Biol.
Sci. 1: 28-31.
21. Lotfi M., Vosoughhosseini S., Ranjkesh B., Khani S., Saghiri
M., Zan V., (2011), Antimicrobial efficacy of nanosilver,
sodium hypochlorite and chlorhexidine gluconate against
Enterococcus faecalis. Afr. J. Biotechnol. 10: 6799-6803.
22. Zarei M., Jamnejad A., Khajehali E., (2014), Antibacterial
effect of silver nanoparticles against four foodborne
pathogens. Jundishap. J. Microbiol. 7: e8720.
23. Ahangaran M. G., Firouzabadi M. S., Firouzabadi M. S.,
(2012), Evaluation of antiseptic role of one nanosilver
based drug as a new therapeutic method for treatment
of Bumblefoot in Pheasant (Phasianus colchicus). Global
Veterinaria. 8: 73-75.
24. Cheng L., Zhang K., Weir M. D., Liu H., Zhou X., Xu H. K.,
(2013), Effects of antibacterial primers with quaternary
ammonium and nano-silver on Streptococcus mutans
impregnated in human dentin blocks. Dent. Mater. 29:
462-472.
25. Shahverdi A. R., Fakhimi A., Shahverdi H. R., Minaian S.,
(2007), Synthesis and effect of silver nanoparticles on
the antibacterial activity of different antibiotics against
Staphylococcus aureus and Escherichia coli. Nanomed.
Nanotechnol. 3: 168-171.
26. Wijnhoven S. W. P., Peijnenburg W. J. G. M., Herberts C. A.,
Hagens W. I., Oomen A. G., Heugens E. H. W., (2009), Nano-
silver: A review of available data and knowledge gaps in
human and environmental risk assessment. Nanotoxicol.
3: 109-138.
27. ElechiguerraJ.L.,BurtJ.L.,MoronesJ.R.,Camacho-Bragado
A., Gao X., Lara H. H., Yacaman M. J, (2005), Interaction of
silver nanoparticles with HIV-1. J. Nanobiotechnol. 3: 6-10.
28. Mehrbod P., Motamed N., Tabatabaian M., Soleimani
Estyar R., Amini E., Shahidi M., (2009), In vitro antiviral
effect of Nanosilver on influenza virus. Daru. 17: 88-93.
29. Naghsh N., Safari M., Hajmehrabi P., (2012), Comparison of
nanosilver inhibitory effects growth between Aspergillus
niger and E. coli. Indian J. Sci. Technol. 5: 2448-2450.
30. Kim K. J., Sung W. S., Moon S. K., Choi J. S., Kim J. G., Lee
D. G., (2008), Antifungal effect of silver nanoparticles on
dermatophytes. J. Microbiol. Biotechnol. 18: 1482–1484.
31. Wang J., Wen L., Wang Z., Chen J., (2006), Immobilization
of silver and nanotubes and their antibacterial effects.
Mater. Chem. Phys. 96: 90-97.
32. Zhong L., Hu J., Cui Z., Wan L., Song W., (2007), In-situ
loading of noble metal nanoparticles on hydroxyl-group-
rich titania precursor and their catalytic applications.
Chem. Mater. 19: 4557-4562.
33. Wei H., Li J., Wang Y., Wang E., (2007), Silver nanoparticles
coated with adenine: Preparation, self-assembly and
application in surface-enhanced Raman scattering.
Nanotechnol. 18: 175610-175615.
34. Deng Z., Chen M., Wu L., (2007), Novel method to fabricate
SiO2
/Ag composite spheres and their catalytic, surface-
enhanced raman scattering properties. J. Phys. Chem. C.
111: 11692-11698.
35. BhattacharyaR.,MukherjeeP.,(2008),Biologicalproperties
of “naked” metal nanoparticles. Adv. Drug Deliv. Rev. 60:
1289-1306.
36. Jia X., Ma X., Wei D., Dong J., Qian W., (2008), Direct
formation of silver nanoparticles in cuttlebone-derived
organic matrix for catalytic applications. Colloids Surf. A:
Physicochem. Eng. Aspects. 330: 234-240.
37. Cho M., Chung H., Choi W., Yoon J., (2005), Different
inactivation behaviors of MS-2 phage and Escherichia
coli in TiO2
photocatalytic disinfection. Appl. Environ.
Microbiol. 71: 270-275.
38. Li Q., Mahendra S., Lyon D., Brunet L., Liga M., Li D.,
Alvarez P., (2008), Antimicrobial nanomaterials for water
disinfection and microbial control: Potential applications
and implications. Water Res. 42: 4591-4602.
39. Sondi I., Salopek-Sondi B., (2004), Silver nanoparticles as
antimicrobial agent: A case study on E. coli as a model for
Gram-negative bacteria. J. Colloid Interf. Sci. 275: 177–182.
40. Baker C., Pradhan A., Pakstis L., Pochan D. J., Shah S. I.,
(2005), Synthesis and antibacterial properties of silver
nanoparticles. J. Nanosci. Nanotechnol. 5: 244-249.
41. Morones, J. R., Elechiguerra, J. L., Camacho, A., Holt, K.,
Kouri, J. B., Ramírez, J. T., Yacaman, M. J., (2005), The
bactericidal effect of silver nanoparticles. Nanotechnol.
16: 2346–2353.
42. Pal S., Tak Y. K., Song J. M., (2007), Does the antibacterial
activity of silver nanoparticles depend on the shape of the
nanoparticle? A study of the gram-negative bacterium
Escherichia coli. Appl. Environ. Microbiol. 73: 1712–1720.
16. 33Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
43. Kim J. S., Kuk E., Yu K. N., Kim J. H., Park S. J., Lee H. J., Kim S.
H., Park Y. K., Park Y. H., Hwang C. Y., (2007), Antimicrobial
effects of silver nanoparticles. Nanomedicine. 3: 95-101.
44. Sanpui P., Murugadoss A., Prasad P. V., Ghosh S. S.,
Chattopadhyay A., (2008), The antibacterial properties of
a novel chitosan-Ag-nanoparticle composite. Int. J. Food
Microbiol. 124: 142–146.
45. Nanda A., Saravanan M. (2009), Biosynthesis of
silver nanoparticles from Staphylococcus aureus and
its antimicrobial activity against MRSA and MRSE.
Nanomedicine. 5: 452–456.
46. Pal S., Yoon E. J., Tak Y. K., Choi E. C., Song J. M., (2009),
Synthesis of highly antibacterial nanocrystalline trivalent
silver polydiguanide. J. Am. Chem. Soc. 131: 16147–16155.
47. Kalishwaralal K., Kanth B. M. S. Pandian S. R., Deepak
V., Gurunathan S., (2009), Silver nanoparticles impede
the biofilm formation by Pseudomonas aeruginosa and
Staphylococcus epidermidis. Colloid Surf. B. 79: 340–344.
48. Parashar U. K., Kumar V., Bera T., Saxena P. S., Nath G.,
Srivastava S. K., R. Giri, Srivastava, A., (2011), Study of
mechanism of enhanced antibacterial activity by green
synthesis of silver nanoparticles. Nanotechnol. 22: 415104-
415108.
49. Esteban-Tejeda L., Malpartida F., Esteban-Cubillo, A.
Pecharroman C., Moya J. S., (2009), The antibacterial and
antifungal activity of a soda-lime glass containing silver
nanoparticles. Nanotechnol. 20: 085103-085107.
50. Jain J., Arora S., Rajwade J. M., Omray P., Khandelwal S.,
Paknikar K. M., (2009), Silver nanoparticles in therapeutics:
Development of an antimicrobial gel formulation for
topical use. Mol. Pharm. 6: 1388–1401.
51. Gajbhiye M., Kesharwani J., Ingle A., Gade A., Rai M.,
(2009), Fungus-mediated synthesis of silver nanoparticles
and their activity against pathogenic fungi in combination
with fluconazole. Nanomedicine. 5: 382–386.
52. Panacek A., Kolar M., Vecerova R., Prucek R., Soukupova J.,
Krystof V., Hamal P., Zboril R., Kvitek L., (2009), Antifungal
activity of silver nanoparticles against Candida spp.
Biomaterials. 30: 6333–6340.
53. Monteiro D. R., Silva S., Negri M., Gorup L. F., De Camargo
E. R., Oliveira R., Barbosa D. B., Henriques M., (2012), Silver
nanoparticles: Influence of stabilizing agent and diameter
on antifungal activity against Candida albicans and Candida
glabrata biofilms. Lett. Appl. Microbiol. 54: 383–391.
54. Krishnaraj C., Ramachandran R., Mohan K., Kalaichelvan
P. T., (2012), Optimization for rapid synthesis of silver
nanoparticles and its effect on phytopathogenic fungi.
Spectrochim. Acta A. 93: 95–99.
55. Gopinath V., Velusamy P., (2013), Extracellular biosynthesis
of silver nanoparticles using Bacillus sp GP-23 and
evaluation of their antifungal activity towards Fusarium
oxysporum. Spectrochim. Acta A. 106: 170–174.
56. Monteiro D. R., Silva S., Negri M., Gorup L. F., De Camargo
E. R., Oliveira R., Barbosa D. B., Henriques M., (2013),
Antifungal activity of silver nanoparticles in combination
with nystatin and chlorhexidine digluconate against
Candida albicans and Candida glabrata biofilms. Mycoses.
56: 672–680.
57. Mishra S., Singh B. R., Singh A., Keswaniv, Naqvi A. H., Singh
H. B., (2014), Biofabricated silver nanoparticles act as a
strong fungicide against Bipolaris sorokiniana causing spot
blotch disease in wheat. PLoS ONE. 9: e97881.
58. Ogar A., Tylko G., Turnau K., (2015), Antifungal properties
of silver nanoparticles against indoor mould growth. Sci.
Total Environ. 521: 305–314.
59. Lok C. N., Ho C. M., Chen R., He Q. Y., Yu W. Y., Sun H., Tam
P. K., Chiu J. F., Che C. M., (2006), Proteomic analysis of
the mode of antibacterial action of silver nanoparticles. J.
Proteome Res. 5: 916–924.
60. Zodrow K., Brunet L., Mahendra S., Li D., Zhang A., Li Q.,
Alvarez P. J., (2009)., Polysulfone ultrafiltration membranes
impregnated with silver nanoparticles show improved
biofouling resistance and virus removal. Water Res. 43:
715–723.
61. Xiang D. X., Chen Q., Pang L., Zheng C. L., (2011), Inhibitory
effects of silver nanoparticles on H1N1 influenza A virus in
vitro. J. Virol. Methods. 178: 137–142.
62. Fayaz A. M., Ao Z., Girilal M., Chen L., Xiao X., Kalaichelvan
P., Yao X., (2012), Inactivation of microbial infectiousness
by silver nanoparticles-coated condom: A new approach
to inhibit HIV- and HSV-transmitted infection. Int. J.
Nanomed. 7: 5007–5018.
63. Trefry J. C., Wooley D. P., (2013), Silver nanoparticles inhibit
vaccinia virus infection by preventing viral entry through
a macropinocytosis-dependent mechanism. J. Biomed.
Nanotechnol. 9: 1624–1635.
64. Xiang D. X., Zheng Y., Duan W., Li X., Yin J., Shigdar S.,
O’Connor M. L., Marappan M., Zhao X., Miao Y., (2013),
Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza
virus infection by silver nanoparticles in vitro and in vivo.
Int. J. Nanomed. 8: 4103–4113.
65. Gaikwad S., Ingle A., Gade A., Rai M., Falanga A., Incoronato
N., Russo L., Galdiero S., Galdiero M., (2013), Antiviral
activity of mycosynthesized silver nanoparticles against
herpes simplex virus and human parainfluenza virus type
3. Int. J. Nanomed. 8: 4303–4314.
66. Khandelwal N., Kaur G., Chaubey K. K., Singh P., Sharma S.,
Tiwari A., Singh S. V., Kumar N., (2014), Silver nanoparticles
impair Peste des petits ruminants virus replication. Virus
Res. 190: 1–7.
67. Orlowski P., Tomaszewska E., Gniadek M., Baska P.,
Nowakowska J., Sokolowska J., Nowak Z., Donten M.,
Celichowski G., Grobelny J., (2014), Tannic acid modified
silver nanoparticles show antiviral activity in herpes
simplex virus type 2 infection. PLoS ONE. 9: e104113.
68. Swathy J. R., Sankar M. U., Chaudhary A., Aigal S., Anshup,
P. T., (2014), Antimicrobial silver: An unprecedented anion
effect. Sci. Rep. 4: 7161-7165.
69. Eming S. A., Krieg T., Davidson J. M., (2007), Inflammation
in wound repair: Molecular and cellular mechanisms. J.
Investig. Dermatol. 127: 514–525.
70. Wong C. K., Cheung P. F., Ip W. K., Lam C. W., (2007),
Intracellular signaling mechanisms regulating toll-like
receptor-mediated activation of eosinophils. Am. J. Respir.
Cell Mol. Biol. 37: 85–96.
71. Broughton G., Janis J. E., Attinger C. E., (2006), The basic
science of wound healing. Plast. Reconstr. Surg. 117:
12s–34s.
72. Witte M. B., Barbul A., (1997), General Principles of wound
healing. Surg. Clin. N. Am. 77: 509–528.
73. Bhol K. C., Schechter P. J., (2007), Effects of nanocrystalline
silver (NPI 32101) in a rat model of ulcerative colitis. Dig.
Dis. Sci. 52: 2732–2742.
74. Tian J., Wong K. K., Ho C. M., Lok C. N., Yu W. Y., Che C.
M., Chiu J. F., Tam P. K., (2007), Topical delivery of silver
nanoparticles promotes wound healing. Chem. Med.
17. 34
P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
Chem. 2: 129-136.
75. Nadworny P. L., Landry B. K., Wang J., Tredget E. E., Burrell
R. E., (2010), Does nanocrystalline silver have a transferable
effect? Wound Repair Regen. 18: 254–265.
76. David L., Moldovan B., Vulcu A., Olenic L., Perde-Schrepler
M., Fischer-Fodor E., Florea A., Crisan M., Chiorean I.,
Clichici S., (2014), Green synthesis, characterization and
anti-inflammatory activity of silver nanoparticles using
European black elderberry fruits extract. Colloids Surf. B.
Biointerf. 122: 767–777.
77. Carmeliet P., Jain R. K., (2000), Angiogenesis in cancer and
other diseases. Nature. 407: 249–257.
78. Timar J., Dome B., Fazekas K., Janovics A., Paku S., (2001),
Angiogenesis-dependent diseases and angiogenesis
therapy. Pathol. Oncol. Res. 7: 85-94.
79. Kalishwaralal K., Banumathi E., Pandian R. K. S., Deepak
V., Muniyandi J., Eom S. H., Gurunathan S., (2009), Silver
nanoparticles inhibit VEGF induced cell proliferation and
migration in bovine retinal endothelial cells. Colloids Surf.
B Biointerf. 73: 51–57.
80. Kemp M. M., Kumar A., Mousa S., Dyskin E., Yalcin M.,
Ajayan P., Linhardt R. J., Mousa S. A., (2009), Gold and
silver nanoparticles conjugated with heparin derivative
possess anti-angiogenesis properties. Nanotechnol. 20:
455104-455109.
81. Gurunathan S., Lee K. J., Kalishwaralal K., Sheikpranbabu
S., Vaidyanathan R., Eom S. H., (2009), Antiangiogenic
properties of silver nanoparticles. Biomater. 30: 6341–
6350.
82. Sriram M. I., Kanth S. B. M., Kalishwaralal K., Gurunathan
S., (2010), Antitumor activity of silver nanoparticles in
Dalton’s lymphoma ascites tumor model. Int. J. Nanomed.
5: 753–762.
83. Kim H., Choi J.S., Kim K. S., Yang J. A., Joo C. K., Hahn S.
K., (2012), Flt1 peptide-hyaluronate conjugate micelle-like
nanoparticles encapsulating genistein for the treatment of
ocular neovascularization. Acta Biomater. 8: 3932–3940.
84. Li C. Y., Zhang Y. J., Wang M., Zhang Y., Chen G., Li L., Wu
D., Wang Q., (2014), In vivo real-time visualization of tissue
blood flow and angiogenesis using Ag2
S quantum dots in
the NIR-II window. Biomater. 35: 393–400.
85. Baharara J., Namvar F., Mousavi M., Ramezani T.,
Mohamad R., (2014), Anti-angiogenesis effect of biogenic
silver nanoparticles synthesized using Saliva officinalis on
chick chorioalantoic membrane (CAM). Molecules. 19:
13498–13508.
86. Thorley A. J., Tetley T. D., (2013), New perspectives in
nanomedicine. Pharmacol. Ther. 140: 176–185.
87. Gopinath P., Gogoi S. K., Chattopadhyay A., Ghosh S. S.,
(2008), Implications of silver nanoparticle induced cell
apoptosis for in vitro gene therapy. Nanotechnol. 19:
075104-075108.
88. AshaRani P. V., Mun G. L. K., Hande M. P., Valiyaveettil S.,
(2009), Cytotoxicity and genotoxicity of silver nanoparticles
in human cells. ACS Nano. 3: 279–290.
89. Jun B. H., Noh M. S., Kim J., Kim G., Kang H., Kim M. S., Seo
Y. T., Baek J., Kim J. H., Park J., (2010), Multifunctional silver-
embedded magnetic nanoparticles as SERS nanoprobes
and their applications. Small. 6: 119–125.
90. Wang H. J., Yang L., Yang H. Y., Wang K., Yao W. G., Jiang
K., Huang X. L., Zheng Z., (2010), Antineoplastic activities
of protein-conjugated silver sulfide nano-crystals with
different shapes. J. Inorg. Biochem. 104: 87–91.
91. Sanpui P., Chattopadhyay A., Ghosh S. S., (2011), Induction
of apoptosis in cancer cells at low silver nanoparticle
concentrations using chitosan nanocarrier. ACS Appl.
Mater. Interfaces. 3: 218–228.
92. Boca S. C., Potara M., Gabudean, A. M., Juhem A, Baldeck
P. L., Astilean S., (2011), Chitosan-coated triangular silver
nanoparticles as a novel class of biocompatible, highly
effective photothermal transducers for in vitro cancer cell
therapy. Cancer Lett. 311: 131–140.
93. Guo D., Zhu L., Huang Z., Zhou H., Ge Y., Ma W., Wu J., Zhang
X., Zhou X., Zhang Y., (2013), Anti-leukemia activity of
PVP-coated silver nanoparticles via generation of reactive
oxygen species and release of silver ions. Biomater. 34:
7884–7894.
94. Gurunathan S., Han J. W., Eppakayala V., Jeyaraj M., Kim
J. H. (2013), Cytotoxicity of biologically synthesized silver
nanoparticles in MDA-MB-231 human breast cancer cells.
BioMed Res. Int. Article ID: 535796.
95. Gurunathan S., Jeong J. K., Han J. W., Zhang X. F., Park J. H.,
Kim J. H., (2015), Multidimensional effects of biologically
synthesized silver nanoparticles in Helicobacter pylori
Helicobacter felis and human lung (L132) and lung
carcinoma A549 cells. Nanoscale Res. Lett. 10: 1–17.
96. Locatelli E., Naddaka M., Uboldi C., Loudos G., Fragogeorgi
E., Molinari V., Pucci A., Tsotakos T., Psimadas D., Ponti
J., (2014), Targeted delivery of silver nanoparticles and
alisertib: In vitro and in vivo synergistic effect against
glioblastoma. Nanomedicine. 9: 839–849.
97. Ortega F. G., Fernandez-Baldo M. A., Fernandez J. G.,
Serrano M. J., Sanz M. I., Diaz-Mochon J. J., Lorente J. A.,
Raba J., (2015), Study of antitumor activity in breast cell
lines using silver nanoparticles produced by yeast. Int. J.
Nanomed. 10: 2021-2031.
98. Banti C. N., Hadjikakou S. K., (2013), Anti-proliferative and
anti-tumor activity of silver (I) compounds. Metallomics. 5:
569-571.
99. Walser T., Demou E., Lang D. J., Hellweg S., (2011),
Prospective environmental life cycle assessment of
nanosilver t-shirts. Environ. Sci. Technol. 45: 4570–4578.
100. Benn T. M., Westerhoff P., (2008), Nanoparticle silver
released into water from commercially available sock
fabrics. Environ. Sci. Technol. 42: 4133-4139.
101. Yang H., Zhu S., Pan N., (2004), Studying the mechanisms
of titanium dioxide as ultraviolet-blocking additive for
films and fabrics by an improved scheme. J. Appl. Polym.
Sci. 92: 3201–3210.
102. El-Molla M. M., El-Khatib E. M., El-Gammal M. S., Abdel-
fatteh S. H., (2011), Nanotechnology to improve coloration
and antimicrobial properties of silk fabrics. Indian J. Fibre
Textile Res. 36: 266-271.
103. Kathiervelu S. S., (2003). Applications of nanotechnology
in fiber finishing. Synth. Fibres. 32: 20-22.
104. Wong Y. W. H., Yuen C. W. M., Leung M. Y. S., Ku S. K. A., Lam
H. L. I., (2006), Selected applications of nanotechnology in
textiles. AUTEX Res. J. 6: 1-8.
105. Cushen M., Kerry J., Morris M., Cruz-Romero M. E.,
(2012), Cummins, Nanotechnologies in the food industry-
recent developments, risks and regulation. Trends Food
Sci. Tech. 24: 30–46.
106. Huang Y., Chen S., Bing X., Gao C., Wang T., Yuan B., (2011),
Nanosilver migrated into food-Simulating solutions from
commercially available food fresh containers. Packag
Technol. Sci. 24: 291–297.
18. 35Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
P. Verma and SK. Maheshwari
107. Couch L. M., Wien M., Brown J. L., Davidson P., (2016),
Food nanotechnology: Proposed uses, safety concerns
and regulations. Agro. Food Ind. Hitech. 27: 36-39.
108. Mihindukulasuriya S. D. F., Lim L. T., (2014),
Nanotechnology development in food packaging: A
review. Trends Food Sci. Technol. 40: 149–167.
109. Pinto R. J. B., Daina S., Sadocco P., Neto C. P., Trindade
T., (2013), Antibacterial activity of nanocomposites of
copper and cellulose. BioMed Res. Int. 6: 280512-280516.
110. Galvez A., Abriouel H., Lopez R. L., Omar N. B., (2007),
Bacteriocinbased strategies for food biopreservation. Int.
J. Food Microbiol. 120: 51–70.
111. Schirmer B. C., Heiberg R., Eie T., Møretrø T., Maugesten
T., Carlehøg M., (2009), A novel packaging method with
a dissolving CO2
headspace combined with organic
acids prolongs the shelf life of fresh salmon. Int. J. Food
Microbiol. 133: 154–160.
112. Soares N. F. F., Silva C. A. S., Santiago-Silva P., Espitia P.
J. P., Gonçalves M. P. J. C., Lopez M. J. G., (2009), Active
and intelligent packaging for milk and milk products, in
engineering aspects of milk and dairy products, eds J.S.R.
Coimbra and J. A. Teixeira (New York, NY: CRC Press),
155–174.
113. Bradley E. L., Castle L., Chaudhry Q., (2011), Applications
of nanomaterials in food packaging with a consideration
of opportunities for developing countries. Trends Food
Sci. Technol. 22: 603-610.
114. Tan H., Ma R., Lin C., Liu Z., Tang T., (2013), Quaternized
chitosan as an antimicrobial agent: antimicrobial activity,
mechanism of action and biomedical applications in
orthopedics. Int. J. Mol. Sci. 14: 1854–1869.
115. Bai Y. X., Li Y. F., Yang Y., Yi L. X., (2006), Covalent
immobilizationoftriacylglycerollipaseontofunctionalized
nanoscale SiO2
spheres. Process Biochem. 41: 770–777.
116. Augustin M. A., and Hemar Y., (2009), Nano- and
micro-structured assemblies for encapsulation of food
ingredients. Chem. Soc. Rev. 38: 902-912.
117. Yoon K. Y., Byeon J. H., Park C. W., Hwang J., (2008),
Antimicrobial Effect of Silver Particles on Bacterial
Contamination of Activated Carbon Fibers. Environ. Sci.
Technol. 42: 1251-1255.
118. Jung J. H., Hwang G. B., Lee J. E., Bae G. N., (2011),
Preparation of airborne Ag/CNT hybrid nanoparticles
using an aerosol process and their application to
antimicrobial air filtration. Langmuir. 27: 10256-10264.
119. Miaskiewicz-peska E., Lebkowska M., (2011), Effect of
antimicrobial air filter treatment on bacterial survival.
Fibers Textiles East. Eur. 19: 73-77.
120. De Gusseme B., Sintubin L., Hennebel T., Boon N.,
Verstraete W., Baert L., Uyttendaele M., (2010), 4th
Int.
Conf. on B/ioinform. Biomed. Engin. Chengdu, China,
18–20 pp 1–5.
121. Pradeep T., (2009), Noble metal nanoparticles for water
purification: A critical review. Thin Solid Films. 517: 6441-
6478.
122. Lv Y., Liu H., Wang Z., Liu S., Hao L., Sang Y., Liu D., Wang
J., Boughton R. I., Membr J., (2009), Silver nanoparticle-
decorated porous ceramic composite for water treatment.
J. Membrane Sci. 331: 50-57.
123. Yakub I., Soboyejo W. O., (2012), Silver nanoparticles:
Synthesis, properties, toxicology, applications and
perspectives. J. Appl. Phys. 111: 124324-12329.
124. Jain P., Pradeep T., (2005), Potential of silver nanoparticle-
coated polyurethane foam as an antibacterial water filter.
Biotechnol. Bioeng. 90: 59-63.
125. Gangadharan D., Harshvardan K., Gnanasekar G., Dixit D.,
Popat K. M., Anand P. S., (2010), The usage of silver as a
disinfectant for water dates back to ancient Greek period.
Water Res. 44: 5481-5487.
126. Sheng Z., Liu Y., (2011), Effects of silver nanoparticles on
wastewater biofilms. Water Res. 45: 6039-6050.
127. Mpenyana-monyatsi L., Mthombeni N. H., Onyango M.
S., (2012), The contamination of groundwater sources by
pathogenic bacteria poses a public. Int. J. Environ. Res.
Public Health. 9: 244-271.
128. Loza K., Diendorf J., Sengstock C., Ruiz-Gonzalez L.,
Gonzalez-Calbet J. M., Vallet-Regi M., Köllerb M., Epple
M., (2014), The dissolution and biological effects of silver
nanoparticles in biological media. J. Mater. Chem. B. 2:
1634–1643.
129. Misra R. D., Girase B., Depan D., Shah J. S., (2012), Hybrid
nanoscale architecture for enhancement of antimicrobial
activity: Immobilization of silver nanoparticles on thiol
functionalized polymer crystallized on carbon nanotubes.
Adv. Eng. Mater. 14: B93–B100.
130. Park M. V., Neigh A. M., Vermeulen J. P., De la Fonteyne L.
J., Verharen H. W., Briede J. J., Van Loveren H., De Jong W.
H., (2011), The effect of particle size on the cytotoxicity,
inflammation, developmental toxicity and genotoxicity of
silver nanoparticles. Biomaterials. 32: 9810–9817.
131. Powers C. M., Badireddy A. R., Ryde I. T., Seidler F. J.,
Slotkin T. A., (2011), Silver nanoparticles compromise
neurodevelopment in PC12
cells: Critical contributions of
silver ion, particle size, coating, and composition. Environ.
Health Perspect. 119: 37–44.
132. Sriram M. I., Kalishwaralal K., Barathmanikanth S.,
Gurunathani S., (2012), Size-based cytotoxicity of silver
nanoparticles in bovine retinal endothelial cells. Nanosci.
Methods. 1: 56–77.
133. Stoehr L. C., Gonzalez E., Stampfl A., Casals E., Duschl A.,
Puntes V., Oostingh G. J., (2011), Shape matters: Effects of
silver nanospheres and wires on human alveolar epithelial
cells. Part. Fiber Toxicol. 8: 36-41.
134. Rycenga M., Cobley C. M., Zeng J., Li W., Moran C. H.,
Zhang Q., Qin D., Xia Y. (2011), Controlling the synthesis
and assembly of silver nanostructures for plasmonic
applications. Chem. Rev. 111: 3669–3712.
135. Suresh A. K., Pelletier D. A., Wang W., Morrell-Falvey J.
L., Gu B. H., Doktycz M. J., (2012), Cytotoxicity induced
by engineered silver nanocrystallites is dependent on
surface coatings and cell Types. Langmuir. 28: 2727–2735.
136. Schlinkert P., Casals E., Boyles M., Tischler U., Hornig E.,
Tran N., Zhao J., Himly M., Riediker M., Oostingh G. J.,
(2015), The oxidative potential of differently charged
silver and gold nanoparticles on three human lung
epithelial cell types. J. Nanobiotechnol. 13: 2-18.
137. Tiyaboonchai W., (2003), Chitosan nanoparticles: A
promising system for drug delivery. Naresuan Univ. J. 11:
51–66.
138. Besinis A., De Peralta T., Handy R. D., (2014), The
antibacterial effects of silver, titanium dioxide and silica
dioxide nanoparticles compared to the dental disinfectant
chlorhexidine on Streptococcus mutans using a suite of
bioassays. Nanotoxicol. 8: 1–16.
139. Agnihotri S., Mukherji S., Mukherji S. (2013), Immobilized
silver nanoparticles enhance contact killing and show
19. 36
P. Verma and SK. Maheshwari
Int. J. Nano Dimens., 10 (1): 18-36, Winter 2019
highest efficacy: Elucidation of the mechanism of
bactericidal action of silver. Nanoscale. 5: 7328–7340.
140. Khurana C., Vala A. K., Andhariya N., Pandey O. P.,
Chudasama B., (2014), Antibacterial activity of silver: The
role of hydrodynamic particle size at nanoscale. J. Biomed.
Mater. Res. A. 102: 3361–3368.
141. Chen Q. C., Jiang H. J., Ye H. L., Li J. R., Huang J. Y., (2014),
Preparation, antibacterial, and antioxidant activities of
silver/chitosan composites. J. Carbohydr. Chem. 33: 298–
312.
142. Shao W., Liu X. F., Min H. H., Dong G. H., Feng Q. Y., Zuo S.
L., (2015), Preparation, characterization, and antibacterial
activity of silver nanoparticle-decorated graphene oxide
nanocomposite. ACS Appl. Mater. Interf. 7: 6966-6973.
143. De Moraes A. C., Lima B. A., De Faria A. F., Brocchi M.,
Alves O. L., (2015), Graphene oxide-silver nanocomposite
as a promising biocidal agent against methicillin-resistant
Staphylococcus aureus. Int. J. Nanomed. 10: 6847–6861.
144. Eckhardt S., Brunetto P. S., Gagnon J., Priebe M., Giese B.,
Fromm K. M., (2013), Nanobio silver: Its interactions with
peptides and bacteria, and its uses in medicine. Chem.
Rev. 113: 4708–4754.
145. Elbeshehy E. K. F., Elazzazy A. M., Aggelis G., (2015),
Silver nanoparticles synthesis mediated by new isolates
of Bacillus spp., nanoparticle characterization and their
activity against Bean Yellow Mosaic Virus and human
pathogens. Front. Microbiol. 6: 453-461.
146. Albanese A., Tang P. S., Chan W. C., (2012), The effect
of nanoparticle size, shape, and surface chemistry on
biological systems. Annu. Rev. Biomed. Eng. 14: 1–16.
147. Rajakumar G., Rahuman A. A., (2012), Acaricidal activity
of aqueous extract and synthesized silver nanoparticles
from Manilkara zapota against Rhipicephalus (Boophilus)
microplus. Res. Veterinary Sci. 93: 303–309.
148. Borase H. P., Patil C. D., Sauter I. P., Rott M. B., Patil S. V.,
(2013), Amoebicidal activity of phytosynthesized silver
nanoparticles and their in vitro cytotoxicity to human
cells. FEMS Microbiol. Lett. 345: 127–131.
149. SathyavathiR.,KrishnaM.B.,RaoS.V.,SarithaR.,Narayana
Rao D., (2010), Biosynthesis of silver nanoparticles using
Coriandrum Sativum leaf extract and their application in
nonlinear optics. Adv. Sci. Lett. 3: 138–143.
150. Balavigneswaran C. K., Kumar T. S. J., Packiaraj R. M.,
Prakash S., (2014), Rapid detection of Cr (VI) by AgNPs
probe produced by Anacardium occidentale fresh leaf
extracts. Appl. Nanosci. 4: 367–378.
151. Raja K., Saravanakumar A., Vijayakumar R., (2012),
Efficient synthesis of silver nanoparticles from Prosopis
juliflora leaf extract and its antimicrobial activity using
sewage. Spectrochim. Acta Part A: Molec. Biomol.
Spectros. 97: 490–494.
152. Suvith V. S., Philip D., (2014), Catalytic degradation of
methylene blue using biosynthesized gold and silver
nanoparticles. Spectrochim. Acta Part A: Molec. Biomol.
Spectros. 118: 526–532.
153. Jebakumar Immanuel Edison T. N., Sethuraman M. G.,
(2013), Electrocatalytic reduction of benzyl chloride by
green synthesized silver nanoparticles using pod extract
of Acacia Nilotica. ACS Sust. Chem. Eng. 1: 1326–1332.
154. Kumar P., Govindaraju M., Senthamil S. S., Premkumar
K., (2013), Photocatalytic degradation of methyl orange
dye using silver (Ag) nanoparticles synthesized from Ulva
lactuca. Colloids and Surf. B: Biointerf. 103: 658–661.
155. Ashok Kumar S, Ravi S., Velmurugan S., (2013), Green
synthesis of silver nanoparticles from Gloriosa superba L.
leaf extract and their catalytic activity. Spectrochim. Acta
Part A: Molec. Biomol. Spectros. 115: 388–392.
156. Waghmode S., Chavan P., Kalyankar V., Dagade S. (2013),
Synthesis of silver nanoparticles using Triticum aestivum
and its effect on peroxide catalytic activity and toxicology.
J. Chem. Article ID. 265864.
157. Gangula A., Podila R., Ramakrishna M., Karanam L.,
Janardhana C., Rao A. M., (2011), Catalytic reduction of
4-nitrophenol using biogenic gold and silver nanoparticles
derived from Breynia rhamnoides. Langmuir. 27: 15268–
15274.