The document discusses the synthesis of silver nanoparticles (AgNPs) using an extract of the water chestnut plant (Trapa natans). It describes how the AgNPs were characterized using techniques like UV-Vis spectroscopy, FTIR, XRD, SEM and TEM. In vitro studies showed the AgNPs exhibited cytotoxic effects against MCF-7 breast cancer cells in a dose-dependent manner with an IC50 of 26 μg/mL. The AgNPs also induced apoptosis in cancer cells and inhibited biofilm formation of bacteria like E. coli and K. pneumoniae at concentrations over 75%. Overall, the study demonstrates the potential of water chestnut-mediated AgNPs for cancer therapy and as antibiofilm agents