Biotechnology applications in agriculture can help improve water security. Advances in embryo technology, cloning, and transgenic animals can lead to smarter livestock farming practices. This includes using techniques like ICSI and embryo transfer to rescue rare and infertile animals or produce livestock with desirable traits. Somatic cell nuclear transfer can also be used to conserve species. Additional biotechnologies such as induced pluripotent stem cells may allow the production of human organs in animals through knockout of specific genes, helping regenerative biomedicine and pharmaceutical production. Overall, smart applications of biotechnology in agriculture can enhance sustainable development and water security.
A cell line is a product of immortal cells that are used for biological research.
Cells used for cell lines are immortal, that happens if a cell is cancerous.
The cells can perpetuate division indefinitely which is unlike regular cells which can only divide approximately 50 times.
Human cell lines
MCF-7 breast cancer
HL 60 Leukemia
HEK-293 Human embryonic kidney
HeLa Henrietta lacks
Primate cell lines
Vero African green monkey kidney epithelial cells
Cos-7 African green monkey kidney cells
And others such as CHO from hamster, sf9 & sf21 from insect cells.
Like all technologies, biotechnology offers the potential of enormous benefit but also potential risks. Biotechnology could help address many global problems, such as climate change, an aging society, food security, energy security and infectious diseases, to name just a few.human health and animal health and welfare and increasing livestock productivity. Biotechnology improves the food we eat - meat, milk and eggs. Biotechnology can improve an animal's impact on the environment. And biotechnology enhances ability to detect, treat and prevent diseases.
A cell line is a product of immortal cells that are used for biological research.
Cells used for cell lines are immortal, that happens if a cell is cancerous.
The cells can perpetuate division indefinitely which is unlike regular cells which can only divide approximately 50 times.
Human cell lines
MCF-7 breast cancer
HL 60 Leukemia
HEK-293 Human embryonic kidney
HeLa Henrietta lacks
Primate cell lines
Vero African green monkey kidney epithelial cells
Cos-7 African green monkey kidney cells
And others such as CHO from hamster, sf9 & sf21 from insect cells.
Like all technologies, biotechnology offers the potential of enormous benefit but also potential risks. Biotechnology could help address many global problems, such as climate change, an aging society, food security, energy security and infectious diseases, to name just a few.human health and animal health and welfare and increasing livestock productivity. Biotechnology improves the food we eat - meat, milk and eggs. Biotechnology can improve an animal's impact on the environment. And biotechnology enhances ability to detect, treat and prevent diseases.
Transgenic manipulation of animal embryos and its applicationDeveshMachhi
INTRODUCTION
Genetic manipulation in animal for higher productivity is also called genetic engineering, refer to the alteration of the gene of an organism.
Organisms containing integrated sequences of cloned dna (transgenes), transferred using techniques of genetic engineering (to include those of gene transfer and gene substitution) are called transgenic animals.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.Genetically modified animals are proving ever more vital in the development of new treatments and cures for many serious diseases.
Transgenesis is a radically new technology for altering the characteristics of animals by introducing the foreign genetic material.
CONTACT: devmac1323@gmail.com
The use of genetic engineering technology in animals has been associated with ethical issues, some of which relate to animal welfare. Discuss examples of genetically engineered animals and evaluate the ethical concerns of genetic engineering.
Transgenic manipulation of animal embryos and its applicationDeveshMachhi
INTRODUCTION
Genetic manipulation in animal for higher productivity is also called genetic engineering, refer to the alteration of the gene of an organism.
Organisms containing integrated sequences of cloned dna (transgenes), transferred using techniques of genetic engineering (to include those of gene transfer and gene substitution) are called transgenic animals.
Transgenic technology has led to the development of fishes, live stock and other animals with altered genetic profiles which are useful to mankind.Genetically modified animals are proving ever more vital in the development of new treatments and cures for many serious diseases.
Transgenesis is a radically new technology for altering the characteristics of animals by introducing the foreign genetic material.
CONTACT: devmac1323@gmail.com
The use of genetic engineering technology in animals has been associated with ethical issues, some of which relate to animal welfare. Discuss examples of genetically engineered animals and evaluate the ethical concerns of genetic engineering.
A transgenic animal is one whose genome has been altered by the transfer of a gene or genes from another species or breed.
Methods involved in production of transgenic animals:
DNA microinjection
Embryonic stem cell-mediated gene transfer and
Retrovirus-mediated gene transfer.
Applications of Transgenic animals:
As disease model
Drug and Industrial production
Disease control
Xenotransplantation
Blood replacement
Agriculture
As food
Transgenic animals are used in toxicity testing.
Transgenic animals are used for vaccine testing
Improving Animal Modeling with 24/7 Home Cage Monitoring in Bioexclusion & Bi...InsideScientific
https://insidescientific.com/webinar/improving-animal-modeling-24/7-home-cage-monitoring-bioexclusion-biocontainment-mouse-housing-system-tecniplast
Recently, a surging response to the COVID-19 pandemic has led to an exponential increase in study support for biocontainment and bioexclusion research. Mouse models are being rapidly developed in both areas, and biosafe housing of these animal models is critical. Additionally, non-invasive home cage monitoring can improve the translational value of these research models.
Locomotor activity patterns can be monitored 24/7 as a diagnostic tool for biosecurity studies. Researchers, staff and animals alike will also benefit from a decreased need for animal handling, caging manipulations and animal monitoring.
This webinar will be most valuable for institutions where biocontainment and bioexclusion work is being considered or conducted, and for researchers who wish to better understand what can be achieved through continuous measurement of animal welfare based of use of non-invasive activity monitoring.
Non-Viral Φc31 Integrase Mediated In Vivo Gene Delivery to the Adult Murine Kidney by Daniel C Chung, Matthew C Canver, Xiaofeng Zuo and Jean Bennett and Joshua H Lipschutz* in Experimental Techniques in Urology & Nephrology
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Applications of Biotechnology in Agriculture for Water Security, Nguyen Van Thuan
1. Water Security for Sustainable Development in a Changing Climate
Applications of Biotechnology in
Agriculture For Water Security
Nguyen Van Thuan, Ph.D
Laboratory for cellular Reprogramming
School of Biotechnology - International University
2. Human Bio-Organs
Stem Cell Therapy
Highest Quality
Products for Agriculture
Genome
Preservation
Assisted Reproductive
Technology (ART)
Preimplantation genetic diagnosis (PGD) Bio-medicine
Research areas of Cellular Reprogramming Laboratory
Lab for Genomic Reprogramming
3. Water Security for Sustainable Development in a Changing Climate
Applications of Biotechnology in
Agriculture For Water Security
Nguyen Van Thuan, Ph.D
Laboratory for cellular Reprogramming
School of Biotechnology - International University
4. Why do we need the responsibility to protect WATER on
our earth
Freshwater Use
Rivers, lakes &
groundwater
0.25% of
earth’s
water
70%
20%
8%
Industry
The earth’s WATER
0.01% of
earth’s
water
Useable
Earth’s water useable is very small and not as infinite as we thought
5. Why do we need the responsibility to protect WATER on
our earth
Drought Flood
We'll need to use water much more smartly
6. Why do we need the responsibility to protect WATER on
our earth
Loss of 15-20 %
of body water
we will death
SMART Water use is very important
Without water for
1 week, human
will be death
Water in human body
7. SMART Water use in Agriculture
In industry: SMART ENERGY In Agriculture: SMART Horticulture
SMART Livestock Farming??
9. SMART Water Use in Livestock Farming
Traditional livestock farming
Smart livestock farming
Applications of high-
biotechnology to the farm
10.
11. 1. Advances in embryos technology:
intracytoplasmic sperm injection (ICSI)
Advanced Biotechnologies in Animals
2. Advances in cloning animals: Applications
in Agriculture
3. Advances in transgenic animals
Applications in Agriculture & in Medicine
4. Advances in animal biotechnology in
regenerative biomedicine: Pharming
12. Nguyen Van Thuan, Ph.D.
Department of Bioscience & Biotechnology
Konkuk University, Seoul, Korea
vanthuan@konkuk.ac.kr
Advance Embryo
Technology
14. What is Embryo Technology?
Embryo Technology is technology
based on Embryology
Embryological sciences:
• Fertilization
• Characteristic of embryos development
• Zygotic gens activation
• Epigenetic reprogramming during embryo development
• Differentiation of embryos
To study Embryo Technology
18. Preservation for Mouse Spermatozoa
Without Freezing
vanthuan@konkuk.ac.kr
Nguyen Van Thuan, Ph.D
Department of Animal Biotechnology
Konkuk University
Van thuan et al. BOR 2005
19. Development of normal mice from oocytes
injected with freeze-dried spermatozoa
Instant sperm:
Just add water
Wakayama T, and Yanagimachi R. 1998.
20. Live/dead cell staining
Fresh sperm
After freeze-dried
Freeze-dried sperm were all died
Wakayama T, and Yanagimachi R. 1998.
21. However…
Freeze-dried sperm can be activated oocytes
and supported full-term development after ICSI.
40
30
20
10
0
1 day 1 week 1 month 3 months
%
200 C (Room temperature)
40 C
Wakayama et al. Nat Bio 1998.
世界初!フリーズドライ精子から生まれたマウス
Dry-mon
22. For that reasons
In order to make freeze-dried
sperm, we still need liquid
nitrogen, freeze-dry system….
However,
Offspring derived from dead sperm by ICSI
BIOLOGY OF REPRODUCTION, 2005
23. Applications of ICSI
- Sperm preservation
- Embryo preservation
- Embryo transfer
- Transgenic Animals
- Treatment of infertile diseases
- etc…
Assisted Reproductive Techniques (ART)
24. 24
Rescues VN Bos Garus (bò tót Việt Nam)
Bos gaurus dead on
May 26, 2014 at
Quang Nam
May 30, 2014
Tinh trùng bò tót
(Spermatozoa of Bos gaurus)Can activation oocytes
25. Water Security for Sustainable Development in a Changing Climate
Advances in somatic cell nuclear
transfer (SCNT): Cloning animals
Nguyen Van Thuan, Ph.D
Laboratory for cellular Reprogramming
School of Biotechnology - International University
26. Somatic Cell Nuclear Transfer: Cloned Animals
Skin cells
(Differentiated cells)
Reprogramming
Stem cells
(Undifferentiated cells)
Dolly, the word’s first cloned animal, 1996
1997 1998 2000 2000 2002 2002 2003 2003 2005 2009
Center for Developmental Biology Japan
Dr. Wakayama
Dr. Kishigami
Genomic Reprogramming Lab. 2002
Nature Protocols, 2006
Production of cloned mice by somatic cell nuclear transfer
Satoshi Kishigami, Sayaka Wakayama, Van Thuan Nguyen, Hiroshi Ohta, Eiji Mizutani,
Takafusa Hikichi, Hong-Thuy Bui, Sebastian Balbach, Atsuo Ogura, Michele Boiani
and Teruhiko Wakayama.
Histone deacetylase inhibitor scriptaid rescues full-term
development in cloned inbred mice by enhancing nascent
mRNA production.
Reproduction 2009
Van Thuan Nguyen , Hong-Thuy Bui, Takafusa Hikichi, Sayaka Wakayama, Satoshi Kishigami,
Eiji Mizutani and Teruhiko Wakayama.
27. Somatic cell nucleus
donor
Oocyte
donor
Oocyte
collection
Donor cells
collection
Enucleation
Remove donor
cell cytoplasm
Activation with
CB for 6 hrs
Embryo
culture
Surrogate
mother
Embryo
transfer
Cloned mice
Caesarian section at
19.5dpc
Nuclear
transfer
Subsequently
culture for 1-2
hr
ntES cells
Bl
Embryo
culture
Success rate is
very low 1-2%
Somatic Cell Nuclear Transfer: Cloned Animals
29. The cytoplasm of mouse germinal vesicle (GV) stage
oocytes can enhance somatic cell nuclear reprogramming
Development. 2008
Bui HT, Wakayama S, Kishigami S, Kim JH, Van Thuan N
Mouse GV
oocytes
extract
Mouse
fibroblast
cells
Treatment
Mouse
fibroblast
cells
Somatic cell
nuclear transfer
Mouse GV extract treatment improve the full-term
development of cloned mouse
Oct4
2 3 4SC ES
DMEM medium
1 2 3 4
ES medium
1
LMNA
GAPDH
Weeks
GV extracts induce expression of Oct-4, and repression of LMNA
Mouse
fibroblast
cells
Mouse GV
oocytes
extract
Treatment
Somatic Cell Nuclear Transfer: Cloned Animals
30. Epigenetic reprogramming in somatic cells induced by
extract from germinal vesicle stage pig oocytes
Development. 2012
Hong-Thuy Bui, D-N Kwon, M-H Kang, M-H Oh, M-R Park, W-J Park, S-S Paik, N. Van Thuan
Pluripotent stem cells
Pig GV
oocytes
extract
In vivo
redifferentiate into
three germ layers
Pig
fibroblast
cells
Somatic Cell Nuclear Transfer: Cloned Animals
31. 25
generation
Cloned
generation 1
Cloned
generation 2
We are the first time can increase the success
rate of cloned animals from 1% to 10%
Tế bào cơ thể
2012
However, the success rate of cloned
mouse still low
Histone deacetylase inhibitor scriptaid rescues full-term
development in cloned inbred mice by enhancing nascent
mRNA production.
Reproduction 2009
Van Thuan Nguyen , Hong-Thuy Bui, Takafusa Hikichi, Sayaka Wakayama, Satoshi Kishigami,
Eiji Mizutani and Teruhiko Wakayama.
Somatic Cell Nuclear Transfer: Cloned Animals
32. The first time on the
world we have successes
25 generations cloned
mice from a body cell.
Sayaka Wakayama, Takashi Kohda, Haruko Obokata,
Mikiko Tokoro, Yukari Terashita, Eiji Mizutani,
Nguyen Van Thuan, Satoshi Kishigami, Fumitoshi Ishino,
and Teruhiko Wakayama
Successful Serial Recloning in the
Mouse over Multiple Generations
CELL (Cell Stem Cell) 2013
Somatic Cell Nuclear Transfer: Cloned Animals
34. 2.5 years old GFP-ICR male
Our unpublished data
Cloned GFP-ICR
Rescue infertile age male mouse via
Cloning Technique
35. Cloned female mice from donor cells of 3
year old ICR female by scriptaid treatment Our unpublished data
36. Next generations from male and female infertile
Rescue infertile age female mouse via
Cloning Technique
Our unpublished data
37. Rescue Rare Animals (Bos gaurus)
Somatic cell nuclear transferBos gaurus dead on
May 26, 2014 at
Quang Nam
Bos gaurus
cloned
blastocyst
This research is funded by Vietnam National University
HoChiMinh City (VNU-HCM) under grant number
B2016-28-01.
38. Application of Cloning Technique
Production of healthy cloned mice from bodies
frozen at -20 degrees C for 16 years.
Wakayama et al, PNAS 2008
39. Advances in Somatic cell Reprogramming
1. Induced pluripotent stem cells: iPS cells
OCT4 Sox2 c-Myc Klf4
Mouse
fibroblast
cells
Kazutoshi Takahashi & Shinya Yamanaka
Cell, 2006
Induction of Pluripotent Stem Cells from Mouse Embryonic and
Adult Fibroblast Cultures by Defined Factors
40. Differentiation and transplantation of functional
pancreatic beta cells generated from induced pluripotent
stem cells derived from a type 1 diabetes mouse model.
Stem Cells and Development. 2012
Jeon K, Lim H, Van Thuan N et al.,
OCT4 Sox2 c-Myc Klf4
Diabestes
Mouse
fibroblast
cells
iPS Cells
Transplantation into
diabetes mouse
Decreases
glucose
levels
Differentiation into
pancreatic cells
Pancreatic cells
Advances in Somatic cell Reprogramming
41. Offspring derived from iPS cell
Reprogramming by iPS cell
iPS cells will inject into
tetraploid embryos. Can
we get offspring derived
from iPS cells???
??
42. Tetraploid
blastocyst (4n)
Injection
ES cells
Fetus was developed
from ES cells
Placenta was developed
from tetraploid embryo
Chromosome remodeling and differentiation of
tetraploid embryos during preimplantation
development.
Part MR, Lee AR, Bui HT, Park KK, Cho SG, Song H, Van Thuan N
Developmental Dynamics, 2011.
Offspring derived from iPS cell
44. Wagyu male Cow
from Australia
Wagyu female cow
from Australia
High quality bull
High quality cow
Cloning animal technique
Cloning Animal Technique
Tế bào từ lổ tai
Animal with high quality of meat
This research is funded by Vietnam National Project
(2016-2021)
45. The process of using transgenic-
cloned animals to produce human
therapeutic proteins (Human drugs)
100.000.000 USD/Cow
Animals Pharming
46. PHARMING for FARMACEUTICALS
Classic method to produce
human therapeutic proteins
Novel method to produce
human therapeutic proteins
Human proteins
producing genes
Bacterial DNA
Plasmid
DNA
Plasmid
DNA cut
with
restriction
enzymes
Recombinant
DNA
Recombinant
Bacterium
Fermentation Tank
Human
proteins
Human
proteins
Human proteins
producing genes
Positive, negative selection
Negative
(GFP-)
Positive
(GFP+)
DNA
Transfection
Dairy cow cells
Cloned
transgenic
embryo
Embryos
transfer
Human
proteins
Cloning animal
Transgenic cow
47. Supper dairy cow
Great business in Animal Farming (Pharming)
Therapeutic Proteins Animal $/animal/year
AAT (alpha-1-antitrysin) Cow
150.000 USD/cow/year
tPA (Tissue plasminogen
activator, chống đông máu)
Cow
700.000 USD/cow/year
Factor VIII or IX or (Blood
clotting factors, điều trị chống
chảy máu).
Cow
200.000 -300.000
USD/cow/year
Lactoferrin 25.000 USD/cow/year
CFTR (Cystic fibrosis
transmembrane conductance
regulator)
Cow
800.000 USD/cow/year
Human Protein C (Chống đông
máu)
Cow 10 milions
USD/cow/year
Fibrinogen (điều trị vết thương) Cow
400.000 USD/cow/year
Glutamic acid decarboxylase
(Điều trị tiểu đường type 1)
Cow
200.000 USD/cow/year
Anpha-lactalbumin (điều trị
nhiễm trùng)
Cow
500.000 USD/cow/year
100.000.000
USD/cow
48. PHARMING
In 2009, we succeeded transgenic pig
Human Erythropoietin (hEPO) gene,
and that transgenic pig shown to have
a normal reproductive ability
1 2
Konkuk University, Sep 24, 2009
Full-term development of
human erythropoietin
transgenic cloned pigs
50. Animals organs for human: Bio-organ
Our group has succeed in making the
korean's first 1,3-galactosyltransferase-
knockout cloned miniatures pigs. This pig
organs will be used for xenotransplantation
into human without immune rejection .
For
Xenotransplantation
(pig’s organs to human)
51. Mouse Pdx1 -/-
(Blastocyst) Rat (ES cells)
Chimeric mouse
with rat pancreas
Generation of RAT pancreas in Pdx1 knockout mice
Kobayashi et al., Cell 2010
0ur hypothesis: Can we make
human organ from animal bodys??
Animals organs for human: Bio-organ
52. Pig fibroblasts cells
Pig enucleated
oocyte
siRNA
Transfection Lentiviral
vector
Pig knockout
pancreas, heart,
liver, kidney related
genes cells
Organ related genes
knockout cloned pig
blastocyst
Human
iPS cells
Embryo
transfer
Humans
organs
?
?
?
?
?
?
? ? ??
Cloned
pig with
human
organs
Animals organs for human: Bio-organ
53. Business of
20 century
Conclusion: SMART LIVESTOCK FARMING
Business of
21 century
Biotechnology
for human
SMART Livestock Farming
for WATER security
54. Thank you for your attention
Cellular Reprogramming Lab Members