This document provides information about Anti-Virus Biomolecular Discovery services from Creative Biolabs for developing antibodies and peptides against infectious diseases. It outlines their platform which includes expression systems for membrane protein production, VLP immunization strategies, and DNA immunization. Their technologies allow for antigen-specific B lymphocyte sorting, monoclonal antibody generation through hybridoma or phage display, and high-density peptide array screening to discover functional antibody and peptide candidates against various viruses such as SARS-CoV-2, HIV, hepatitis, and influenza.

1. The leading custom service provider in antibody development and engineering
Anti-Virus Biomolecular
Discovery
To Discovery Best-in-class Biomolecular Therapies for Infective Diseases
Web: www.creative-biolabs.com
Email: info@creative-biolabs.com
Address: 203, 17 Ramsey Road, Shirley, NY 11967, USA

2. 1. Virus of Infectious Diseases
2. Viral Pathogenesis
3. Our Platform
4. Main Technologies
CONTENTS

3. Virus of Infectious Diseases

4. Introduction to Viruses
Image From Wikimedia Commons
1 Virus of Infectious Diseases

5. The Baltimore Classification of viruses
Image From Wikimedia Commons
1 Virus of Infectious Diseases

6. Viral Life Cycle
Image From Wikipedia
1. Attachment
2. Penetration
3. Uncoating
4. Transcription
5. Synthesis
6. Assembly
7. Release
1 Virus of Infectious Diseases

7. The Virus that causes infectious diseases
SARS-CoV-2 HIV
Hepatitis Virus
HPAI
Rabies Virus
MERS-CoV
Zika Virus
Ebola Virus
SARS-CoV
Enterovirus 71
Dengue Virus Influenza Virus
Creative Biolabs provides antibody and peptide discovery services against the
above viruses.
1 Virus of Infectious Diseases

8. Viral Pathogenesis

9. Introduction to Viral Pathogenesis
2 Viral Pathogenesis
Loss of cell function
Kill cells
Tissue damage caused by host immune responses
Complex combination of direct damage & host
immune responses

10. Routes of Virus Entry
2 Viral Pathogenesis
Entry via the Respiratory Tract
Entry via the Gastrointestinal Tract
Entry via the Skin
Entry via Other Routes
Image From Fenner’s Veterinary Virology. DOI:10.1016/B978-0-12-375158-4.00003-1

11. 2 Viral Pathogenesis
Neural spread
Hematogenous Spread
Local Spread on Epithelial Surfaces
Subepithelial Invasion and Lymphatic Spread
Mechanisms of Viral Spread and Infection

12. Mechanisms of Virus Shedding
2 Viral Pathogenesis
Via budding
Via apoptosis
Via exocytosis
Image From Fenner’s Veterinary Virology. DOI:10.1016/B978-0-12-375158-4.00003-1 Image From Wikipedia

13. Virus Transmission
2 Viral Pathogenesis
Fecal-oral Respiratory Droplets Fomites-mediated
Exchange of Bodily
Fluids or Organs
Airborne Insect Vectors

14. Mechanisms of Viral Injury and Disease
2 Viral Pathogenesis
Image From Fenner’s Veterinary Virology. DOI:10.1016/B978-0-12-375158-4.00003-1
Inhibition of Host-Cell Nucleic Acid
Synthesis
Inhibition of Host-Cell RNA
Transcription
Inhibition of Processing of Host-Cell
Messenger RNAs
Inhibition of Host-Cell Protein
Synthesis

15. Neutralizing Antibody Targets of Viral Disease
2 Viral Pathogenesis
Take AIDS as an example
CD4 binding site (CD4bs) V2 site
Membrane proximal external
region (MPER)
N332 supersite
Gp120–gp41 interface

16. Our Platform

17. Antibodies directed against membrane proteins for neutralization of virus infectivity are highly sought for
therapeutic, diagnostic, and research purposes. Creative Biolabs has developed a series of cell-based systems and
cell-free systems for virus membrane protein expression.
Membrane Protein Production and Immunization Platform
Creative Biolabs has established a broad range of expression systems including but not
limited to E. coli system, yeast system, insect system, plant system, mammalian system.
We have accumulated extensive experience in anti-virus antibody development by using
VLP immunization strategy.
VLPs Production and Immunization Platform
Creative Biolabs can offer a series of DNA immunization
strategies to develop high-affinity antibodies against the
natural conformation of the target antigen.
DNA Immunization Platform
Anti-Virus Biomolecular Discovery
3 Our Platform

18. Ph.D. level scientists with
extensive experience
Cost-effective and short turnaround
Board-spectrum targets
Various strategies
Diverse species available
High-throughput functional molecules
screening
Large pool of functional
antibody/peptide candidates
AntInfect™ Platform
3 Our Platform

19. Main Technologies

20. Antigen-Specific B Lymphocytes Sorting Technology
Fluorescent activated cell sorting (FACS) Laser-capture Microdissection (LCM)
4 Main Technologies

21. Generation of Monoclonal Antibodies
Hybridoma Technology
Abdullah Farhan ul haque Saeed, et al. Antibody Engineering for Pursuing a Healthier Future. Front Microbiol. 2017, 8,
495. doi:10.3389/fmicb.2017.00495
4 Main Technologies

22. Phage Display Technology
4 Main Technologies

23. High-Density Peptide Array
Michael Hecker, et al. High-Density Peptide Microarray Analysis of IgG Autoantibody Reactivities in Serum and Cerebrospinal Fluid of
Multiple Sclerosis Patients. Molecular & Cellular Proteomics. 2016,15(4): 1360-1380.
4 Main Technologies

24. T H A N K S
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https://www.creative-biolabs.com