Seminario de Biología Molecular

1. Valery Leilany Briñez Prada
Third Semester of medicine
Universidad Pontificia Bolivariana

2. Illustration
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Medical
Art
- Single cell microorganism
- Gene expresión at a high level
- Post-translational modifications
- Heterologus expression.
- Supports the inmune system.
Saccharomyces cerevisae
Experimental system
Easy genomic manipulation

3. FOOT AND MOUTH
DISEASE VIRUS
Hand, foot and mouth disease is a highly
contagius disease caused by FMD Virus. It
causes mouth ulcers, and spots on the hands
and feet.

4. Illustration
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Medical
Art
Engineer Saccharomyces Cerevisae
to express structural proteins of
FMDV and produce VLPs.
OBJETIVE

5. Materials and Methods
Construction of yeast expresion vectors and
transformation
Northern blot analysis
Fusion gene construct specifically designed to
provide the necessary genetic elements for
yeast cells to express these FMDV genes.
Technique that helps analyze the expresion of
specific genes by detecting and quantifying
the corresponding RNA molecules from the
engineered yeast cells.

6. Materials and Methods
Western blot analysis Inmune response of expressed VLPs
.
Technique that helps analyze the expresion of
specific genes by detecting and quantifying
specific proteins from the engineered yeast
cells.
Activation of the system against these particle
mimics in order to generate a strong inmune
response without causing disease
(Recognization)

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Medical
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Resultados

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Resultados

9. Author Statement Validation of article
Rodriguez and Grubman
(2009)
“Empty capsid vaccines, such as viruslike
particles (VLPs), while more complicated than
simple subunit vaccines, are a fascinating
option, since they preserve the epitope
structures without employing viruses during the
vaccine manufacturing process”
Felipe et al. 2003; Donnelly
et al. 2001; Sharma et al.
2012
“We demonstrated a high expression level of
FMDV structural proteins and the proteolytic
process associated with the structural
polyprotein of FMDV using S. cerevisiae”
(Blandin et al. 2000; Brachat
et al. 2003; Cliften et al.
2001; Dunham et al. 2002;
Garfnkel 2005; Gofeau et al.
1996, 1997)
“Baker’s yeast Saccharomyces cerevisiae has
been an attractive experimental system for
basic research because of their great advances
such as the huge amount of currently available
genetic, molecular, and cellular information”
Discussion

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Molecular Biology Techniques
Conclusions
Development of Virus-Like Particles
signify a transformative era in
medicine. Enhacing our ability to
respond rapidly to emerging
infectious diseases.
Virus like particles
Precise manipulation of genetic material
enhances sensitivity and specificity in
detecting pathogens or biomarkers.
Optimizing medical efficacy.

11. Concept Map