This document evaluates the antibacterial activity of ethanolic extracts of leaves and roots of Passiflora foetida. The aim was to test the extracts against various bacteria like E. coli, Bacillus subtilis, Pseudomonas putida, Staphylococcus pyogenes, and Klebsiella pneumoniae using the cup plate method. The results showed that the leaf extract had larger zones of inhibition compared to the root extract and standard antibiotics tetracycline and kanamycin, indicating that components in P. foetida leaves have potent antibacterial activity against both gram-positive and gram-negative bacteria. The study reveals the promising antibacterial properties of P. foetida and further research on it is warranted

1. ANTIBACTERIAL ACTIVITY OF LEAF AND ROOT
EXTRACT OF PASSIFLORA FOETIDA L
PRESENTED BY:
DINTAKURTHI MOHAN KRISHNA*
UNDER THE GUIDANCE OF
RAVINDRAN SARAVANAN M. PHARM
QIS COLLEGE OF PHARMACY
ONGOLE

2. INTRODUCTION
THE ANTIBACTERIAL ACTIVITY OF MANY DRUGS IS PROGRESSIVELY
DECREASING AS THE MICROBES ARE DEVELOPING RESISTANCE TO
THE DRUGS.
THE QUEST FOR NEWER ANTIBACTERIAL WITH EFFICIENT
ANTIBACTERIAL ACTIVITY IS GOING ON.
IN ANCIENT TIMES, PASSIFLORA FOETIDA WAS EMPLOYED IN
TRADITIONAL MEDICINE FOR VARIOUS THERAPEUTIC ACTIONS.
PASSIFLORA FOETIDA WAS SELECTED FOR ANTIBACTERIAL
TESTING IN HOPE OF DEVELOPING A NEW ANTIBACTERIAL DRUG.

3. Aim and scope of the work
• THE AIM OF STUDY WAS TO EVALUATE THE
ANTIBACTERIAL ACTIVITY OF ETHANOLIC
EXTRACTS OF LEAF & ROOT OF PASSIFLORA
FOETIDA

4. PASSIFLORA FOETIDA - PROFILE
PASSIFLORA FOETIDA WIDELY DISTRIBUTED PLANT IN INDIA
BELONGS TO FAMILY : PASSIFLORACEAE
 SYNONYM : WILD WATERMELON
TRADITIONALLY USED AS : ANTI-DIABETIC,
ANTI-INFLAMMATORY
ANALGESIC.
COLLECTION OF PLANTS AND LEAVES WAS DONE IN AND AROUND
ONGOLE.

5. EXTRACTION
 METHOD OF EXTRACTION :
CONTINUOUS HOT PERCOLATION
 APPARATUS EMPLOYED :
SOXHLET APPARATUS
 PLANT PART EXTRACTED :
LEAVES AND ROOTS
 SOLVENTS USED :
ETHANOL (95%)

6. EVALUATION OF ANTIBACTERIAL ACTIVITY
METHODOLOGY
Medium : Mueller-Hinton Agar Medium (Himedia)
Method : Cup plate method
Standard drug : Tetracyclin (30 µg/100 µl)
Kannamycin (30 µg/100 µl)
Incubation : 37 oC for 24-48 hrs.
Micro-organisms used
E.Coli Bacillus subtilis pseudomonas staphylococcus . Klebsiella pneumoniae
putida pyogenes

7. EXTRACTS OF PASSIFLORA FOETIDA L LEAVES AND ROOT
DILUTIONS FOR PRODUCT A(LEAF EXTRACT) &
PRODUCT B(ROOT EXTRACT)
250µg/100µl
500µg/100µl
750µg/100µl
1000µg/100µl
Incubation :
The inoculated plates were incubated at 37ºC for 24 - 48 hrs.
After incubation, the zone of inhibitions were measured

8. ANTIBACTERIAL ACTIVITY
Zone of inhibitions{in mm}
From the above data ,Product A(Leaf extract) shows BETTER
zone of inhibition when compared against Product B(Root
extract),and standards Tetracyclin,Kanamycin

9. ANTIBACTERIAL ACTIVITY
20
25
23 23
20
22
23
18
23
20
24
20
22
20
22
18
22
18
20
18
0
5
10
15
20
25
30
Escheriachia coli Bascillus subtilis Pseudomonas putida Staphylococcus pyogenes Klebsiella pneumoniae
ZoneofInhibitioninmm
Microrganism
Antibacterial activity
Leaf extract
Root extract
Tetracyclin
Kanamycin

10. CUP PLATE METHOD

11. CONCLUSION
THE ABOVE RESULT WAS COMPARED WITH ZONE OF INHIBITION PRODUCE BY
STANDARD ANTIBIOTICS LIKE TETRACYCLIN (30ΜG) - 25 MM AND KANAMYCIN
(30ΜG)- 18 MM. IT CLEARLY SHOWS THAT THE ISOLATED COMPONENTS OF
PASSIFLOREA FOETIDA ARE IN PROGRESS AS POTENT AGENTS AGAINST BOTH
GRAM POSITIVE AND GRAM NEGATIVE MICROBES.
THE SCREENING OF PASSIFLORA FOETIDA L REVEALED
PROMISING ANTI-BACTERIAL PROPERTIES.
PASSIFLORA FOETIDA L CAN BE FURTHER RESEARCHED FOR
ANTI-BACTERIAL STUDIES.

12. REFERENCE
• 1. BUSHRA BEEGUM NR AND GANGA DEVI T: ANTI BACTERIAL ACTIVITY OF
SELECTED SEA WEEDS FROM KOVALAM SOUTH WEST COAST OF INDIA.
• 2. CASTELLO MC, PHATAK A, CHANDRA N, SHARON M: ANTIMICROBIAL
ACTIVITY OF CRUDE EXTRACT.
• 3. SUSEEL L, SARSVATHY A AND BRIDHA P: PHARMACOLOGONOSTIC STUDIES ON
PASSIFLORA FOETIDA.