testing the antimicrobial efficiency of leaf extract of Eupatorium perfoliatum and analyse their phytochemicals

1. Antimicrobial and phytochemical
screening of the leaf extracts
of
Eupatorium perfoliatum

2. INTRODUCTION
 Nature is and will still aid as man’s primary source for the
cure of his disabilities
 However, the potential of higher plants as a source for new
drugs is still largely unexplored
 The traditional system of using medicinal plants for curing
many diseases dates back to the age of Rig Veda.
 Many microbial diseases can be cured by medicinal plants
without any side effects and economical issues.
 The development of microbial resistance to presently
available antibiotics has necessitated searching for the new
antimicrobial agent.
Thus, the present study aims at evaluating the
antimicrobial activity and phytochemical screening of
Eupatorium perfoliatum.

3. Eupatorium
perfoliatum(Boneset)
Kingdom- Plantae
Order- Asterales
Family- Asteraceae
Genus- Eupatorium
Species-perfoliatum
Medicinal Applications
• To treat influenza, swine flu,
acute bronchitis, nasal inflammation, joint
pain (rheumatism), fluid retention, dengue
fever, and pneumonia. as a stimulant and to
cause sweating.

4. EXPERIMENTAL PROCEDURE
Leaves - collected, shade dried and powdered
5g-extracted with petroleum ether, ethanol, chloroform
and acetone separately
The extracts were evaporated and the residue was
dissolved in Dimethyl sulfoxide (DMSO)
Microbial strains - Micrococcus luteus, Serratia
marcescens and Bacillus subtilis (Gram positive bacteria)
and E. coli, Pseudomonas aeruginosa and Enterobacter
faecalis( Gram negative bacteria) and the fungal isolates
namely Aspergillus niger, Aspergillus fumigatus,
Aspergillus flavus and Trichoderma viride

5. Muller Hinton agar media and rose Bengal
chloramphenicol agar - Agar Well Diffusion
 The wells were cut and the microbial cultures were
swabbed on the agar medium and 20µl of each
extract was added to the well seperately and
incubated at 37ºC for 24h (Bacteria) and 25ºC for
96h (Fungi).
DMSO - negative control
chloramphenicol (bacteria) and amphothericin B
(Fungi) – positive control
Phytochemical analysis – alkaloids, carbohydrates,
saponins, phenolic compounds, flavonoids, tannins,
steroids, proteins, aminoacids and glycosides.

6. Results
Antibacterial
activity

7. Micrococcus luteus• Bacillus substilis
Serratia marcescens
Enterobacter faecalis
E. coli
Pseudomonas aeroginosa
1- Chloroform extract 4- Acetone extract
2- Ethanol extract 5- Negative control (DMSO)
3- Petroleum ether extract 6- Positive control ( Chloramphenicol

8. ANTIFUNGAL ACTIVITY
Trichoderma viride
Aspergillus flavus Aspergillus fumigatusAspergillus niger
1. Choloroform extract
2. Petroleum ether extract
3. Ethanol extract
4. Acetone extract
5. Negative Control (DMSO)
6. Positive Control (Amphotericin B)

9. PHYTOCHEMICAL
SCREENING

10. S.No Phytochemicals
Chloroform Extract of
E. perfoliatum
1
Alkaloids
Dragendroff’s reagent test +
Wagner’s reagent test +
Mayer’s reagent test
2
Flavonoids
Schinoda test +
Ammonia test +
3
Phenols +
Ferric chloride test +
Lead acetate test +
4
Proteins and Amino Acids
Ninhydrin test -
Biuret test -
5
Tannins
Breamer’s test +
6
Carbohydrates
Fehling’s test +
7
Glycosides
Borntrager’s test -
8
Saponins
Froth test +

11. CONCLUSION
• Thus the leaves of the Eupatorium
perfoliatum had significant antimicrobial
activity with differences in their magnitude
• But the effective biomolecules which act as
antimicrobial substance have to be isolated
and identified so that it could become the
base for the development of a medicine, a
natural blueprint for the development of
new drugs

12. THANK YOU