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This document summarizes a study that screened 40 plant extracts for antimicrobial activity against Mycobacterium tuberculosis. The study aimed to screen extracts from plants traditionally used to treat tuberculosis and other infections in Sudan. The screening identified 5 extracts that showed distinct antimicrobial properties against M. tuberculosis through high-throughput screening using luciferase to determine bacterial growth and confirmation of inhibition through CFU plating and analysis of bacterial growth. The extracts warrant further study to evaluate their potential as sources of new anti-tuberculosis drugs.
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Antibacterial activity of Isolated Phytochemicals
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ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
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1. Drug interactions occur when one drug alters the effects of another drug through pharmacokinetic or pharmacodynamic mechanisms. Interactions can be harmful by increasing toxicity or decreasing efficacy.
2. Risk factors for interactions include multiple prescribers, genetic factors, and diseases affecting drug metabolism. Interactions can result in loss of therapeutic effects, toxicity, or beneficial additive/antagonist effects.
3. Pharmacokinetic interactions involve effects on absorption, distribution, metabolism by CYP450 enzymes, and excretion of drugs. Pharmacodynamic interactions directly or indirectly alter the pharmacological response to drugs.
Bioactivity-guided Fractionation of Selected Botanticals
This document summarizes research on bioactivity-guided fractionation of selected botanicals, including black chokeberry, maqui berry, mangosteen, and noni. Key findings include:
- Isolates from black chokeberry and maqui berry showed potent antioxidant and quinone reductase induction activities.
- Compounds isolated from mangosteen, including xanthones, exhibited antioxidant and anti-cancer activities. α-Mangostin in particular showed aromatase inhibition.
- The presentation outlined the application of bioactivity-guided fractionation to identify potential cancer chemopreventive compounds from these botanicals.
Preventing extinction and improving conservation status of two important medi...
This document summarizes research on the macropropagation of two threatened medicinal plants: Adhatoda beddomei and Stereospermum tetragonum. The objectives were to standardize propagation methods for both species to aid in their ex-situ conservation. For A. beddomei, stem cuttings treated with IBA rooting hormone showed the highest rooting success in a mixture of garden soil, vermicompost and sand. For S. tetragonum, soaking seeds in distilled water and planting in cocopeat led to the highest germination rates. A positive relationship was also found between pod length and seed number for S. tetragonum. The research aims to help stabilize declining wild
Media monitoring worldwide anti drugs day 2012
To celebrate the Worldwide Anti Drugs Day on 26th June 2012, Indonesia Media Monitoring Center (IMMC) have done a research about these issues.
The research are done in span of a year, starting from 15th June 2011 until 15th June 2012. From this research, a new perspective about dynamics of drug issues in Indonesia can be found. The result of the research will be release in a press release.
ANTIMICROBIAL ACTIVITY OF VARIOUS PLANTS EXTRACTS
The document summarizes a study on the antimicrobial activity of various plant extracts against common pathogenic microorganisms. The study tested aqueous extracts of myrtle, harmal, henna, thyme, and fenugreek against bacteria (Pseudomonas, Klebsiella, E. coli, Proteus, Staphylococcus) and the yeast Candida. The myrtle extract showed the strongest inhibition of all microbes at 5% concentration. The harmal extract inhibited bacteria at 10% concentration except Candida which was inhibited at 20%. The henna extract inhibited bacteria at 20% except Candida was resistant. Thyme only inhibited 2 bacteria at 20% while others were resistant. Fen
Group5 presentation final
The document discusses an Anthrone test performed on soya milk, shredded coconut, and dry yeast to determine the presence of carbohydrates. The test produced a blue-green color for soya milk and shredded coconut, indicating the presence of carbohydrates. Dry yeast produced a red color, showing the absence of carbohydrates. The test was used to analyze different sample types - liquid, solid, and powder.
Anti mycobacterial drugs
Tuberculosis is caused by infection with Mycobacterium tuberculosis. It infects over a billion people worldwide and kills millions each year. A combination of drugs, including isoniazid, rifampin, pyrazinamide, and ethambutol or streptomycin, are used to treat tuberculosis. Isoniazid and rifampin are the most effective drugs but multi-drug therapy is required to prevent resistance. Both drugs have bactericidal effects and penetrate tissues well but can cause adverse reactions like hepatitis which requires monitoring during treatment.
INH drug 'Isoniazid'
Isoniazid is a first-line medication used in the prevention and treatment of tuberculosis. It works by inhibiting the synthesis of mycolic acid. Isoniazid is available in tablet, syrup, and injectable forms. Common side effects include nausea, vomiting, peripheral neuropathy, and potentially fatal hepatitis. Precautions must be taken in patients with hepatic or renal impairment. Treatment requires monitoring for side effects and typically lasts 6-9 months to prevent tuberculosis relapse.
Thesis presentation
This thesis report examines antibiotic resistance due to spices like garlic and turmeric. The objectives were to extract the spices using solvents, test their antimicrobial properties, and determine the minimum inhibitory concentration. Garlic, turmeric, and a combination were extracted using distilled water, ethanol, and chloroform. Extracts showed antibacterial effects against E. coli and Staphylococcus aureus, with ethanol and distilled water being most effective. The minimum inhibitory concentration of extracts was lower than standard antibiotics, indicating they may help reduce antibiotic resistance.
Antitubercular Drugs
This document discusses antimycobacterial drugs used to treat tuberculosis. It begins by introducing mycobacteria, noting their characteristics like being acid-fast and pathogenic. It then lists important mycobacterium species like M. tuberculosis, M. bovis, and M. leprae which cause diseases like TB, leprosy, and Johne's disease. The document outlines mycobacteria virulence factors that help them evade the immune system. It also describes the mycobacterial cell wall and its components. Finally, it discusses methods to diagnose mycobacterial infections, including microscopy, culture, and genomic detection.
Estimation of Carbohydrates by anthrone method
Text Version is Available at: http://www.biochemden.in/2014/11/anthrone-method-carbohydrate-determination.html
Carbohydrates are very important component of Storage and structural materials in the plants. The carbohydrates are stored as free sugars and polysaccharides. The basic units of carbohydrates are Monosaccharides. When hydrolyse the carbohydrates, gives monosaccharides, but when hydrolyse monosaccharides it can not be split into more simpler sugars. The hydrolysed product of Polysaccharide are estimating by the resultant monosaccharides.
Gene therapy - a Powerpoint Presentation
This document discusses gene therapy for the treatment of cancer. It begins by explaining what genes are and how they relate to cancer development. It then describes the process of making gene therapy medicine, which involves using a modified adenovirus to deliver a tumor suppressor gene like p53 into cancer cells. The document explains how gene therapy works by having the adenovirus deliver the tumor suppressor gene into the cancer cell nucleus, where it causes the cancer cell to self-destruct. It notes that gene therapy can be administered directly into tumors or systemically via injection. The document concludes by presenting examples of patients who were
Phytochemical Screening, Isolation & Characterization of the compounds from e...
This document describes research on the isolation and characterization of compounds from the seeds of Annona squamosa. The objectives were to extract compounds from the seeds, perform phytochemical screening of the extracts, and isolate and evaluate the chemical structure of any cardiac glycosides present. The methodology included extraction of the seeds using solvents, phytochemical analysis, thin layer chromatography, column chromatography, and various spectroscopic techniques. Analysis indicated the presence of cardiac glycosides and characterization suggested the isolated compound was a bufadienolide with a probable structure similar to other known cardiac glycosides.
Gene therapy
Gene therapy involves modifying genes to treat or cure disease. It works by replacing mutated genes, inactivating abnormal genes, or introducing new genes. Early successes treated immune deficiencies, but challenges remain in achieving long-term effects without side effects. Promising areas are treating inherited retinal diseases and Parkinson's through localized delivery of therapeutic genes using viral or non-viral vectors. While offering potential cures, gene therapy also raises ethical issues that require ongoing discussion.
Thin layer chromatography
Thin layer chromatography (TLC) is a chromatography technique used to separate mixtures by distributing the components between a stationary phase, such as silica gel coated on a plate, and a mobile phase, such as a solvent mixture, which moves up the plate by capillary action. TLC involves spotting a sample mixture onto the plate, developing it in a solvent system, and visualizing the separated components, which travel at different rates depending on how they partition between the stationary and mobile phases. TLC is a simple, fast, and inexpensive analytical technique used for qualitative and quantitative analysis of organic compounds and testing compound purity.
ANTI-TB AND ANTI LEPROTIC DRUGS [MEDICINAL CHEMISTRY] BY P.RAVISANKAR.
This document provides information about anti-tubercular drugs. It discusses various drugs used to treat tuberculosis (TB) including isoniazid, rifampicin, ethambutol, and pyrazinamide. It describes the mechanisms of action, side effects, dosages, and importance of combination therapy to prevent development of drug resistance in TB treatment.
Disinfection testing for disinfection
The document discusses various methods for testing the efficacy of disinfectants, including:
1. Koch's method, which tests the ability of a disinfectant to kill Bacillus anthraces spores.
2. Rideal Walker and Chick-Martin tests, which determine the phenol coefficient of a disinfectant by comparing its bactericidal effects to phenol under clean and dirty conditions.
3. In-use and capacity use dilution tests assess the ability of a disinfectant to kill microbes when diluted in conditions mimicking actual use over time in the presence of organic matter.
4. No single test can reliably determine a disinfectant's efficacy
Anti tuberculosis drugs
This document provides information on Mycobacterium tuberculosis, the bacteria that causes tuberculosis (TB). It discusses the physiology and structure of M. tuberculosis, how it is transmitted, diagnosed, and its typical appearance under microscopy. The document also categorizes and describes the mechanisms and uses of both first- and second-line anti-tuberculosis drugs, including isoniazid, rifampin, pyrazinamide, ethambutol, and streptomycin from the first line and ethionamide, cycloserine, and thiacetazone from the second line. It addresses drug-resistant TB and the different groupings of alternative drugs used to treat multi- and extensively drug-resistant strains.
Test for protein quantification
Presentation done by me, Rayan osman for the course of Enzymology in the biochemistry major. Submitted to Dr.Roland Bouraad and big thanks to him
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Anti Tubercular Drugs - Mechanism of Action and Adverse effects
Anti Tubercular Drugs - Mechanism of Action and Adverse effects
Bioactivity-guided Fractionation of Selected Botanticals
Bioactivity-guided Fractionation of Selected Botanticals
Preventing extinction and improving conservation status of two important medi...
Preventing extinction and improving conservation status of two important medi...
Phytochemical Screening, Isolation & Characterization of the compounds from e...
Phytochemical Screening, Isolation & Characterization of the compounds from e...
ANTI-TB AND ANTI LEPROTIC DRUGS [MEDICINAL CHEMISTRY] BY P.RAVISANKAR.
ANTI-TB AND ANTI LEPROTIC DRUGS [MEDICINAL CHEMISTRY] BY P.RAVISANKAR.
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Phytochemical screening and antibacterial properties from extract of Alchorne...
This study involved a survey on the use of extract of Alchornea cordifolia a medicinal plant used locally in Cameroon as traditional medicine for the treatment of infectious diseases.
Antimicrobial alpana
The document discusses chemotherapeutic agents and antibiotics. It describes how Paul Ehrlich pioneered the concept of selective toxicity for targeted treatment of diseases. Key discoveries included arsphenamine for syphilis. Gerhard Domagk later discovered sulfonamides. Characteristics of effective antibiotics include appropriate spectrum of activity, low toxicity, and resistance prevention. Methods to determine antibiotic effectiveness include dilution tests to find minimum inhibitory concentrations.
Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether extracts of
Choclospermum planchonii leaf were tested against some clinical bacterial isolates and reference organisms.
The phytochemical screenings of the extracts were also carried out. The leaf extracts of the plant exhibited
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aureus, Shigella flexneri, and Salmonella typhii and reference strains of Staphylococcus aureus (ATCC 28923),
Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853. The methanolic leaf extracts
exhibited the highest antibacterial activity, its minimum inhibitory concentration (MIC) ranging between 0.31
and 1.25 mg/ml; and its zones of inhibition on the various test microorganisms ranging between 8mm and 13
mm. The petroleum ether extracts had the weakest antibacterial activity, its minimum inhibitory concentration
(MIC) ranging between 2.50mg/ml and 5.00mg/ml and its inhibition zone ranging between 4mm and 8mm. The
phytochemical constituents present in the plant were alkaloids, tannins, saponins, cardiac glycosides, and
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indicates that the plant leaf could be useful in the treatment of human infections caused by these pathogens. The
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The Antimicrobial, Antioxidant and Antibacterial Effects of Seven Underexplor...
This study evaluated the antimicrobial, antioxidant, and antibacterial properties of seven medicinal plant extracts. Phytochemical analysis revealed the presence of phenols, flavonoids, and steroids in all plant extracts. The extract of Psychotria peduncularis showed the highest total phenolic and flavonoid content. Antioxidant activity was determined using DPPH, hydroxyl, and nitric oxide radical scavenging assays. The extracts exhibited significant antioxidant effects. Antibacterial activity was evaluated against both bacteria and yeast, with the extracts of Tristemma Mauritianum and P. peduncularis showing the most potent effects. The results suggest that the screened plant extracts may be useful as sources for
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Synergistic antibacterial effects of three edible plants extract against anti...
Synergistic antibacterial effects of three edible plants extract against anti...Open Access Research Paper
In vitro synergistic antibacterial effects among Alocasia macrorrhizos rhizome, Amorphophallus paeoniifolius corm and Colocasia esculenta corm extracts were tested against six resistant bacteria viz., Yersinia enterocolitica, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile and Staphylococcus aureus. The inhibition zone was compared with the commercially available antibiotic (tetracycline). High inhibitory activity was observed against E. coli (12.67±0.33 mm) and S. aureus (12.50±0.29 mm) for methanol extract at 800 mgml-1 of concentration. MIC and MBC of the extracts ranged from 200-580 mgml-1 and 250-650 mgml-1 respectively. The lowest MIC and MBC of the extracts were measured against E. coli.
Origanum vulgare, Nanoemulsion
This study evaluated the anti-acne potential of essential oils (EOs) from seven Mediterranean plants against acne-causing bacteria. Oregano EO showed the strongest antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis based on disc diffusion, MIC, MBC, and MBIC assays. Oregano oil was formulated into a nanoemulsion and demonstrated superior healing and antimicrobial effects compared to a reference antibiotic in a mouse model of acne. The results suggest oregano oil nanoemulsion is a potential natural alternative for treating acne and combating emerging antibiotic resistance.
Antibacterial effectofsomemedicinalplantsagainststaphylococcusaureusandpseudo...
Multidrug resistant microorganisms are globally becoming a major confrontment because of illogical use of antibiotics and this played a good role in investigation about the antibacterial compounds in plants. Thus, the present study investigate for the antibacterial effect of alcoholic extracts of Curcuma longa L. rhizomes , CommiphoramyrrhaL. gums and Ginkgo biloba L. leaves products against Staphylococcus aureus and Pseudomonas aeruginosa. The plants samples extracted by soxhlet with methanol and fractionation with and four solution ( chloroform, hexane, water and ethyl acetate) were used for investigation about antibacterial activity by disc diffusion method. The results showed that methanolic alcohol extract and fractions of C. longa L. rhizomes , C. myrrha L. gums showed biological activity against P. aeruginosa and S. aureus bacteria, but methanolic alcohol extract and fractions of G. biloba L. leaves product didn’t show any activity as antibacterial substance. It can be concluded that the presence of secondary metabolites as flavonoids, alkaloids, tannins, glycosides and saponins in the plants under study would be marked a good anti-bacterial effect.
JCSN 2014 02 Revised Manuscript
The document summarizes a study that investigated the phytochemical composition and antimicrobial activity of dried seed extracts of Maranthes polyandra. Key findings include:
1) Preliminary phytochemical screening revealed the presence of steroidal triterpenes, cardiac glycosides, and trace amounts of alkaloids in the chloroform, ethyl acetate, and methanol extracts.
2) The ethyl acetate extract exhibited the highest inhibition zone (29mm) against Candida stellatoidea. Ethyl acetate and methanol extracts inhibited growth of many test organisms at a concentration of 3.75mg/mL.
3) The minimum bactericidal/fungicidal concentration of most extracts against
antimicrobial chemotherapy
This is a series of lectures on microbiology useful for undergraduate medical and paramedical students
Microbiological culture sensitivity test
Microbiological culture sensitivity tests involve growing microbial organisms in culture media under controlled conditions and testing their sensitivity to drugs. A sample is taken from an infected area and grown on a plate with antibiotics. The size of the zone of inhibition around each antibiotic disk indicates whether the microbe is susceptible, resistant, or intermediate. This helps clinicians select the most effective drug to treat a patient's infection and guide treatment decisions.
Summer 09 Proposal
This proposal aims to isolate and identify the active compounds in five medicinal plants - ginger, hierba buena, poleo, cinnamon, and clavo - that give them antibacterial properties against E. coli, Enterococcus faecalis, and Salmonella enteriditis. The plants will be extracted and purified through chromatography techniques. The isolated compounds will then be tested using disk diffusion assays and minimum inhibitory concentration assays to determine which have the strongest inhibitory effects on the bacteria. Identifying these antibacterial compounds could contribute to the development of natural bactericides and promote further research on medicinal plant compounds.
Invitro Study of Antibacterial Activity of Leaf and Root Extract of Rauvolfia...
Abstract: In this study Methanolic and chloroform leaf and root extract of Rauvolfia serpentina was studied for its antibacterial activity. Antibacterial activity of leaf and root extracts was assessed against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Klebsiella pneumonia by disc diffusion method. Methanolic extract of root was showed the maximum zone of inhibition for all test organisms than the leaf extract. According to observations of root extract of 50µl/ml concentration 15.4mm, 16.2mm, 12.3mm,10.1mm and 15.0mm zones of inhibition and for concentration of 100µl/ml 22.5mm, 23.1mm, 15.1mm, 18.0mm, 22.0mm zones of inhibition were formed against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Klebsiella pneumonia respectively. 50µl/ml concentration of leaf and root chloroform extracts showed no zone of inhibition against Staphylococcus aureus and Bacillus subtilis, maximum zone of inhibition was observed 15.0mm and 15.5mm against E. coli for leaf and root chloroform extract respectively. 100µl/ml concentration showed maximum zone of inhibition against all test organisms for both leaf and root extracts. All the bacteria were more susceptible to methanolic extracts than the chloroform extracts.
Antimicrobial activity of leaf extracts of jurinea dolomiaea plant
The International Institute for Science, Technology and Education (IISTE). Science, Technology and Medicine Journals Call for Academic Manuscripts
Analytical techniques in plant pathology
Agricultural crops are threatened by a wide variety of plant diseases and pests that can damage crops and lower yields. Correct diagnosis of plant diseases is important so that treatments can be tailored to specific pathogens. There are indirect and direct methods used for diagnosis. Indirect methods include environmental reviews and microscopic examination, while direct methods use molecular techniques like antibody-based methods (e.g. ELISA, polyclonal antibodies) and nucleic acid-based methods (e.g. PCR, DNA microarrays) to identify causal agents. DNA microarrays allow screening for multiple pathogens simultaneously. Correct diagnosis is key to minimizing crop losses from diseases and pests.
In Vitro Antibacterial Activities of Cochlospermum planchonii Roots Crude Ext...
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether of
Cochlospermum planchonii root extracts on some clinical bacterial isolates and reference organisms were
investigated using conventional microbiological and microdilution indicator technique. Phytochemical
screenings were also carried on the extracts. The root extracts of the plant exhibited antibacterial activities
against reference strains and clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii. However, the susceptibility pattern of the bacteria did not
differ significantly from each other (p>0.05). The methanolic root extracts exhibited the highest antibacterial
activity, its minimum inhibitory concentration (MIC) ranging between 1.25 mg/ml and 5.00mg/ml; and its zones
of inhibition diameter on the various test microorganisms ranging between 8mm and 12mm. The petroleum
ether extracts had the weakest antibacterial activity, with minimum inhibitory concentration of 5.00mg/ml and
its zones of inhibition diameter ranging between 4mm and 7mm. The bioactive constituents in the plant were
alkaloids, tannins, saponins, cardiac glycosides, and sterols. The methanolic extracts of root appeared to be
more biologically active than other extracts and may be more useful in treating human infections caused by
these pathogens.
Antimicrobial activity of herbal extracts on staphylococcus aureus and propio...
This study evaluated the antimicrobial activity of extracts from three Thai herbs (Excoecaria cochinchinensis, Salvia officinalis, and Argyreia nervosa) against Staphylococcus aureus and Propionibacterium acnes, two bacteria implicated in acne. Ethanolic and water extracts of E. cochinchinensis and S. officinalis showed activity against both bacteria based on well diffusion assays, whereas extracts of A. nervosa were inactive. Minimum inhibitory concentration testing found that water extracts of E. cochinchinensis and S. officinalis were effective against S. aureus and P. acnes at concentrations of 1.56-6.25 mg/ml and
Testing 1492
This document provides guidelines for antimicrobial susceptibility testing. It discusses various testing methods including disk diffusion, dilution, and diffusion/dilution hybrid methods. Key factors that influence testing such as pH, moisture, and divalent cation content are summarized. Recommendations are provided for preparation of Mueller-Hinton agar medium, antibiotic stock solutions, filter paper disks, and bacterial inoculum standardization. The Kirby-Bauer disk diffusion method is described in detail as the recommended procedure.
Testing
1. The document discusses various methods of antimicrobial susceptibility testing including disk diffusion, dilution, and diffusion-dilution methods. It describes the Kirby-Bauer disk diffusion method in detail.
2. Key steps of the Kirby-Bauer method include preparing Mueller-Hinton agar, antibiotic discs, inoculum, and following standard procedures for performing the test and interpreting results.
3. Factors that can influence test results like pH, moisture, divalent cations, and thymidine/thymine content are discussed. Quality control is important for reliable and reproducible outcomes.
Mycoplasma contamination of cell cultures
Biological contamination is the dread of every person working with cell culture. When cultures become infected with microorganisms, or cross-contaminated by foreign cells, these cultures usually must be destroyed. Since the sources of culture contamination are ubiquitous as well as difficult to identify and eliminate, no cell culture laboratory remains unaffected by this concern. With the continuing increase in the use of cell culture for biological research, vaccine production, and production of therapeutic proteins for personalized medicine and emerging regenerative medicine applications, culture contamination remains a highly important issue. Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line. Problems with cell line cross-contamination have even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. Major cell line repositories, including the American Type Culture Collection (ATCC), the European Collection of Cell Cultures (ECACC) and the German Collection of Microorganisms and Cell Cultures (DSMZ), have received cell line submissions from researchers that were misidentified by them. Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions. ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.
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Phytochemical screening and antibacterial properties from extract of Alchorne...
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Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...
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Antibacterial effectofsomemedicinalplantsagainststaphylococcusaureusandpseudo...
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- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Simplify your search for a reliable Python development partner! This list presents the top 10 trusted US providers offering comprehensive Python development services, ensuring your project's success from conception to completion.
Leveraging the Graph for Clinical Trials and Standards
Katja Glaß
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Main news related to the CCS TSI 2023 (2023/1695)
An English 🇬🇧 translation of a presentation to the speech I gave about the main changes brought by CCS TSI 2023 at the biggest Czech conference on Communications and signalling systems on Railways, which was held in Clarion Hotel Olomouc from 7th to 9th November 2023 (konferenceszt.cz). Attended by around 500 participants and 200 on-line followers.
The original Czech 🇨🇿 version of the presentation can be found here: https://www.slideshare.net/slideshow/hlavni-novinky-souvisejici-s-ccs-tsi-2023-2023-1695/269688092 .
The videorecording (in Czech) from the presentation is available here: https://youtu.be/WzjJWm4IyPk?si=SImb06tuXGb30BEH .
5th LF Energy Power Grid Model Meet-up Slides
5th Power Grid Model Meet-up
It is with great pleasure that we extend to you an invitation to the 5th Power Grid Model Meet-up, scheduled for 6th June 2024. This event will adopt a hybrid format, allowing participants to join us either through an online Mircosoft Teams session or in person at TU/e located at Den Dolech 2, Eindhoven, Netherlands. The meet-up will be hosted by Eindhoven University of Technology (TU/e), a research university specializing in engineering science & technology.
Power Grid Model
The global energy transition is placing new and unprecedented demands on Distribution System Operators (DSOs). Alongside upgrades to grid capacity, processes such as digitization, capacity optimization, and congestion management are becoming vital for delivering reliable services.
Power Grid Model is an open source project from Linux Foundation Energy and provides a calculation engine that is increasingly essential for DSOs. It offers a standards-based foundation enabling real-time power systems analysis, simulations of electrical power grids, and sophisticated what-if analysis. In addition, it enables in-depth studies and analysis of the electrical power grid’s behavior and performance. This comprehensive model incorporates essential factors such as power generation capacity, electrical losses, voltage levels, power flows, and system stability.
Power Grid Model is currently being applied in a wide variety of use cases, including grid planning, expansion, reliability, and congestion studies. It can also help in analyzing the impact of renewable energy integration, assessing the effects of disturbances or faults, and developing strategies for grid control and optimization.
What to expect
For the upcoming meetup we are organizing, we have an exciting lineup of activities planned:
-Insightful presentations covering two practical applications of the Power Grid Model.
-An update on the latest advancements in Power Grid -Model technology during the first and second quarters of 2024.
-An interactive brainstorming session to discuss and propose new feature requests.
-An opportunity to connect with fellow Power Grid Model enthusiasts and users.
Dandelion Hashtable: beyond billion requests per second on a commodity server
This slide deck presents DLHT, a concurrent in-memory hashtable. Despite efforts to optimize hashtables, that go as far as sacrificing core functionality, state-of-the-art designs still incur multiple memory accesses per request and block request processing in three cases. First, most hashtables block while waiting for data to be retrieved from memory. Second, open-addressing designs, which represent the current state-of-the-art, either cannot free index slots on deletes or must block all requests to do so. Third, index resizes block every request until all objects are copied to the new index. Defying folklore wisdom, DLHT forgoes open-addressing and adopts a fully-featured and memory-aware closed-addressing design based on bounded cache-line-chaining. This design offers lock-free index operations and deletes that free slots instantly, (2) completes most requests with a single memory access, (3) utilizes software prefetching to hide memory latencies, and (4) employs a novel non-blocking and parallel resizing. In a commodity server and a memory-resident workload, DLHT surpasses 1.6B requests per second and provides 3.5x (12x) the throughput of the state-of-the-art closed-addressing (open-addressing) resizable hashtable on Gets (Deletes).
Introduction of Cybersecurity with OSS at Code Europe 2024
I develop the Ruby programming language, RubyGems, and Bundler, which are package managers for Ruby. Today, I will introduce how to enhance the security of your application using open-source software (OSS) examples from Ruby and RubyGems.
The first topic is CVE (Common Vulnerabilities and Exposures). I have published CVEs many times. But what exactly is a CVE? I'll provide a basic understanding of CVEs and explain how to detect and handle vulnerabilities in OSS.
Next, let's discuss package managers. Package managers play a critical role in the OSS ecosystem. I'll explain how to manage library dependencies in your application.
I'll share insights into how the Ruby and RubyGems core team works to keep our ecosystem safe. By the end of this talk, you'll have a better understanding of how to safeguard your code.
Columbus Data & Analytics Wednesdays - June 2024
Columbus Data & Analytics Wednesdays, June 2024 with Maria Copot 20
Digital Banking in the Cloud: How Citizens Bank Unlocked Their Mainframe
Inconsistent user experience and siloed data, high costs, and changing customer expectations – Citizens Bank was experiencing these challenges while it was attempting to deliver a superior digital banking experience for its clients. Its core banking applications run on the mainframe and Citizens was using legacy utilities to get the critical mainframe data to feed customer-facing channels, like call centers, web, and mobile. Ultimately, this led to higher operating costs (MIPS), delayed response times, and longer time to market.
Ever-changing customer expectations demand more modern digital experiences, and the bank needed to find a solution that could provide real-time data to its customer channels with low latency and operating costs. Join this session to learn how Citizens is leveraging Precisely to replicate mainframe data to its customer channels and deliver on their “modern digital bank” experiences.
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-und-domino-lizenzkostenreduzierung-in-der-welt-von-dlau/
DLAU und die Lizenzen nach dem CCB- und CCX-Modell sind für viele in der HCL-Community seit letztem Jahr ein heißes Thema. Als Notes- oder Domino-Kunde haben Sie vielleicht mit unerwartet hohen Benutzerzahlen und Lizenzgebühren zu kämpfen. Sie fragen sich vielleicht, wie diese neue Art der Lizenzierung funktioniert und welchen Nutzen sie Ihnen bringt. Vor allem wollen Sie sicherlich Ihr Budget einhalten und Kosten sparen, wo immer möglich. Das verstehen wir und wir möchten Ihnen dabei helfen!
Wir erklären Ihnen, wie Sie häufige Konfigurationsprobleme lösen können, die dazu führen können, dass mehr Benutzer gezählt werden als nötig, und wie Sie überflüssige oder ungenutzte Konten identifizieren und entfernen können, um Geld zu sparen. Es gibt auch einige Ansätze, die zu unnötigen Ausgaben führen können, z. B. wenn ein Personendokument anstelle eines Mail-Ins für geteilte Mailboxen verwendet wird. Wir zeigen Ihnen solche Fälle und deren Lösungen. Und natürlich erklären wir Ihnen das neue Lizenzmodell.
Nehmen Sie an diesem Webinar teil, bei dem HCL-Ambassador Marc Thomas und Gastredner Franz Walder Ihnen diese neue Welt näherbringen. Es vermittelt Ihnen die Tools und das Know-how, um den Überblick zu bewahren. Sie werden in der Lage sein, Ihre Kosten durch eine optimierte Domino-Konfiguration zu reduzieren und auch in Zukunft gering zu halten.
Diese Themen werden behandelt
- Reduzierung der Lizenzkosten durch Auffinden und Beheben von Fehlkonfigurationen und überflüssigen Konten
- Wie funktionieren CCB- und CCX-Lizenzen wirklich?
- Verstehen des DLAU-Tools und wie man es am besten nutzt
- Tipps für häufige Problembereiche, wie z. B. Team-Postfächer, Funktions-/Testbenutzer usw.
- Praxisbeispiele und Best Practices zum sofortigen Umsetzen
AppSec PNW: Android and iOS Application Security with MobSF
Mobile Security Framework - MobSF is a free and open source automated mobile application security testing environment designed to help security engineers, researchers, developers, and penetration testers to identify security vulnerabilities, malicious behaviours and privacy concerns in mobile applications using static and dynamic analysis. It supports all the popular mobile application binaries and source code formats built for Android and iOS devices. In addition to automated security assessment, it also offers an interactive testing environment to build and execute scenario based test/fuzz cases against the application.
This talk covers:
Using MobSF for static analysis of mobile applications.
Interactive dynamic security assessment of Android and iOS applications.
Solving Mobile app CTF challenges.
Reverse engineering and runtime analysis of Mobile malware.
How to shift left and integrate MobSF/mobsfscan SAST and DAST in your build pipeline.
GNSS spoofing via SDR (Criptored Talks 2024)
In the realm of cybersecurity, offensive security practices act as a critical shield. By simulating real-world attacks in a controlled environment, these techniques expose vulnerabilities before malicious actors can exploit them. This proactive approach allows manufacturers to identify and fix weaknesses, significantly enhancing system security.
This presentation delves into the development of a system designed to mimic Galileo's Open Service signal using software-defined radio (SDR) technology. We'll begin with a foundational overview of both Global Navigation Satellite Systems (GNSS) and the intricacies of digital signal processing.
The presentation culminates in a live demonstration. We'll showcase the manipulation of Galileo's Open Service pilot signal, simulating an attack on various software and hardware systems. This practical demonstration serves to highlight the potential consequences of unaddressed vulnerabilities, emphasizing the importance of offensive security practices in safeguarding critical infrastructure.
Freshworks Rethinks NoSQL for Rapid Scaling & Cost-Efficiency
Freshworks creates AI-boosted business software that helps employees work more efficiently and effectively. Managing data across multiple RDBMS and NoSQL databases was already a challenge at their current scale. To prepare for 10X growth, they knew it was time to rethink their database strategy. Learn how they architected a solution that would simplify scaling while keeping costs under control.
Fueling AI with Great Data with Airbyte Webinar
This talk will focus on how to collect data from a variety of sources, leveraging this data for RAG and other GenAI use cases, and finally charting your course to productionalization.
Connector Corner: Seamlessly power UiPath Apps, GenAI with prebuilt connectors
Join us to learn how UiPath Apps can directly and easily interact with prebuilt connectors via Integration Service--including Salesforce, ServiceNow, Open GenAI, and more.
The best part is you can achieve this without building a custom workflow! Say goodbye to the hassle of using separate automations to call APIs. By seamlessly integrating within App Studio, you can now easily streamline your workflow, while gaining direct access to our Connector Catalog of popular applications.
We’ll discuss and demo the benefits of UiPath Apps and connectors including:
Creating a compelling user experience for any software, without the limitations of APIs.
Accelerating the app creation process, saving time and effort
Enjoying high-performance CRUD (create, read, update, delete) operations, for
seamless data management.
Speakers:
Russell Alfeche, Technology Leader, RPA at qBotic and UiPath MVP
Charlie Greenberg, host
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Richin final ppt 19
- 1. Screening of 40 Plant extracts for antimicrobial activity against Mycobacterium tuberculosis Richin John Koshy Supervisor: Maria Lerm
- 2. Overview Background Hypothesis/Aim Materials and methods Results Conclusion
- 4. Morphology Mycobacteria are small rod shaped aerobic bacilli Mycobacteria are present in the environment, soil and water Mycobacteria have high concentration of lipids and wax in the cell wall making them resistant to standard staining techniques Mtb grow quite slowly and colonies do not appear on solid media up to 2.5 -5 week Media which are used in growth of mycobacteria are selective media, semisynthetic media and broth media www.lookfordiagnosis.com
- 5. Pulmonary TB
- 7. Treatment of TB niaid.nih.gov
- 8. Drug Development Screening of Chinese herbal medicine led to introduction of artemisinin from the leaves of Artemisia annua which is used worldwide for treatment against malaria At present, there are eight to ten drugs with therapies lasting to 18-24 months of which there are only four drugs which are actually developed to treat TB Shifting the screening strategy from a single enzyme targets to a whole bacterial cell level have been showing successful results as it has a holistic approach and only gives hits that can enter the bacterium through the cell wall
- 9. Hypothesis Whole cell screening method is a more effective way than molecular/chemical approaches to identify new, anti-mycobacterial drug candidates/lead compounds Traditionally used plants are a valuable source for the discovery of modern drugs
- 10. Aim To screen 40plant extracts prepared from plants used traditionally to treat tuberculosis and other infections in Sudan for possible antimicrobial activity against Mtb
- 12. Results Effect of DMSO on plant extracts DMSO at concentration of 10 %used along with plant extracts as treatments for reduction of antimycobacterial growth showing higher pattern of inhibition and a reduction of inhibition seen with DMSO concentration of 1 % and 0.1 %
- 13. Anti-mycobacterial screening of plant extracts against avirulent strain of Mtb H37Ra (1)
- 14. Anti-mycobacterial screening of plant extracts against avirulent strain of Mtb H37Ra (2) Screening of 40 plant extracts by analysis of mycobacterial growth by luminometry and measuring cell viability showing a pattern of inhibition in plant extracts A10, A12, A20, A22, A28, A29, A30, A34
- 15. Confirming screening of plant extracts showing inhibition Student paired t test was done assuming Gaussian distribution which show statistically insignificant hits
- 16. Detecting changes in mycobacterial growth by CFU plating
- 19. 40 extracts of selected medicinal plants were analysed for antimycobacterial activities which yielded 5 extracts to have distinct antimycobacterial properties
- 20. Further studies were carried out in order to measure colonies in the presence of selected medicinal extracts showing inhibition of bacterial growth, thereby ruling out inhibition due to luciferase
- 22. I also express my gratitude to Johanna Raffetseder, Marie Larsson, ElsjePienaarand Daniel Eklundfor introducing me to most of the methods iused in this project and more and also for always being there to answer numerous questions I had.