Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA). A cDNA library represents a sampling of the transcribed genes, but a genomic library includes untranscribed regions.
Introduction
History
Cell culture techniques
Species cloned
Approaches of cell cloning
Monolayer culture- Dilution cloning
Microtitration plate
Suspension culture- Cloning in agar
Cloning in methocel
Isolation of clone
By clonal rings
By suspension clone
Application of cell cloning
Conclusion
Reference
Introduction
Primary Culture
Steps In Primary Culture
Isolation Of Tissue
Dissection And/Or Disaggregation
Types Of Primary Culture
Primary Explant Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
reference
Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA). A cDNA library represents a sampling of the transcribed genes, but a genomic library includes untranscribed regions.
Introduction
History
Cell culture techniques
Species cloned
Approaches of cell cloning
Monolayer culture- Dilution cloning
Microtitration plate
Suspension culture- Cloning in agar
Cloning in methocel
Isolation of clone
By clonal rings
By suspension clone
Application of cell cloning
Conclusion
Reference
Introduction
Primary Culture
Steps In Primary Culture
Isolation Of Tissue
Dissection And/Or Disaggregation
Types Of Primary Culture
Primary Explant Culture
Enzymatic Disaggregation
Mechanical Disaggregation
Cell Line( Finite & Continuous)
Naming A Cell Line
Choosing A Cell Line
Maintenance Of Cell Line
Conclusion
reference
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Sheep named Dolly was cloned by transfer of a nucleus from a mammary (Udder) cell of an adult sheep into an egg cell.
mammary cell
Nucleus
insert into
a egg cell
First demonstration of pluripotency (totipotency) of a nucleus of a differentiated adult cell.
Cloning of dolly somatic cell nuclei
clone cattle, sheep, goats, pigs.
nuclear transfer procedures are similar.
Adult donor cells from a variety of cell types(mammary epithelial and ovarian cells, fibroblasts, lymphocytes) are isolated
Cultured and genetically modified methods.
individual donor cells are fused to an enucleated oocyte with short-duration electric pulse.
eg: two 2.5 kilovolt /cm pulses for 10microseconds
Used to fuse adult cattle fibroblasts with enucleated oocytes.
The pulses simultaneously induce cell fusion and oocyte activation.
Blastocyst stage before transferred into the uterus of a pseudopregant female.
Confirmed transgene at the time of birth
Surviving animals produced by nuclear transfer are healthy.
There, is a substantial loss of individual before and after birth some of the cloned animals display abnormalities.
Abnormlities such as increased birth weight.
Dna methylation and histone modification of the original donor cell is inappropriate maintained in the cells of the recipient animals.
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Biology and characterization of the cell cultureKAUSHAL SAHU
Introduction
History
Important terminology
Biology of culture cell
Characterization of culture cell
Application of animal culture
Conclusion
References
8. Biology and characterization of cultured cellsShailendra shera
Immediate environment and environment of surrounding medium governs the various properties of cell. The in vitro condition markedly affects the cellular property of cultured cells. For e.g. Reduction in Cell–cell and cell-material interaction. Therefore, it is imperative to develop understanding of biology of cells in response to various environmental conditions. Characterization of cells helps to identify the origin, purity and authenticity of cells and cell lines.
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
TYPES OF SYNCHRONIZATION
(I)PHYSICAL CELL SEPARATION
(II)BLOCKADE
PHYSICAL Vs BLOCKADE SYNCHRONIZATION
CONCLUSION
REFFERENCE
Cellular coning refers to generation of genetically identical cells from parent cells. This presentation teaches differences between cell coning and molecular cloning and various methods of cell cloning. Sample questions are also provided for your review of concept learned
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Sheep named Dolly was cloned by transfer of a nucleus from a mammary (Udder) cell of an adult sheep into an egg cell.
mammary cell
Nucleus
insert into
a egg cell
First demonstration of pluripotency (totipotency) of a nucleus of a differentiated adult cell.
Cloning of dolly somatic cell nuclei
clone cattle, sheep, goats, pigs.
nuclear transfer procedures are similar.
Adult donor cells from a variety of cell types(mammary epithelial and ovarian cells, fibroblasts, lymphocytes) are isolated
Cultured and genetically modified methods.
individual donor cells are fused to an enucleated oocyte with short-duration electric pulse.
eg: two 2.5 kilovolt /cm pulses for 10microseconds
Used to fuse adult cattle fibroblasts with enucleated oocytes.
The pulses simultaneously induce cell fusion and oocyte activation.
Blastocyst stage before transferred into the uterus of a pseudopregant female.
Confirmed transgene at the time of birth
Surviving animals produced by nuclear transfer are healthy.
There, is a substantial loss of individual before and after birth some of the cloned animals display abnormalities.
Abnormlities such as increased birth weight.
Dna methylation and histone modification of the original donor cell is inappropriate maintained in the cells of the recipient animals.
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
Introduction
What is cloning?
Why we want to do cloning?
History
Technique of cell cloning
Dolly – the sheep
Species cloned
Why persue animal cloning research?
Conclusion
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Biology and characterization of the cell cultureKAUSHAL SAHU
Introduction
History
Important terminology
Biology of culture cell
Characterization of culture cell
Application of animal culture
Conclusion
References
8. Biology and characterization of cultured cellsShailendra shera
Immediate environment and environment of surrounding medium governs the various properties of cell. The in vitro condition markedly affects the cellular property of cultured cells. For e.g. Reduction in Cell–cell and cell-material interaction. Therefore, it is imperative to develop understanding of biology of cells in response to various environmental conditions. Characterization of cells helps to identify the origin, purity and authenticity of cells and cell lines.
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
TYPES OF SYNCHRONIZATION
(I)PHYSICAL CELL SEPARATION
(II)BLOCKADE
PHYSICAL Vs BLOCKADE SYNCHRONIZATION
CONCLUSION
REFFERENCE
Cellular coning refers to generation of genetically identical cells from parent cells. This presentation teaches differences between cell coning and molecular cloning and various methods of cell cloning. Sample questions are also provided for your review of concept learned
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Normal Labour/ Stages of Labour/ Mechanism of LabourWasim Ak
Normal labor is also termed spontaneous labor, defined as the natural physiological process through which the fetus, placenta, and membranes are expelled from the uterus through the birth canal at term (37 to 42 weeks
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
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Chapter 3 - Islamic Banking Products and Services.pptx
Animal cloning
1. Animal Cloning
By :
Hanz Apple Coscos
Zenia Biong
Reinalyn Andaluz
Elisha Yuipco
Agnes Grace Sabijon
2. What is animalcloning?
A clone is a genetic copy of another living
organism. The genetic material of a cloned
offspring is drawn from a single source, rather
than being a combination of sperm and egg
genes. In sexual reproduction, half of the
genetic material of an individual comes from a
female and half from a male.
3. Advantagesof AnimalCloning
• There will be an endless supply of animals to clone, and we
will never run out of food from animals, because we have
been able to clone based on previous efforts, the most
famous of these was the first ever cloning of an animal,
Dolly the lamb which was a successful cloning where Dolly
wasa healthylamb.
4. • The animal in which we intend to clone will result perfectly
the same as the animal which has been cloned in every way,
identical in all senses of the word. The eyes, the nose, the
ears, the face,everything!Dolly was a perfect example of this
through how she came out exactly the same. But this sheep
is not the only animal which cloning can work effectively on,
there are many animals which have also been successfully
cloned such as horses and bulls, justnot as famous as Dolly.
5. • Through animal cloning, we can go into further research into
how complicated and intricate our world that God created really
is, and could discover informationthatwe’ve never seen before.
• One of the greatest breakthroughs of all time, cloning has been
discovered, something which could be revolutionary if we use it
to our advantage through continuing on with our research and
studies into it, with discoveries that could change our lives
forever.
6. Disadvantagesof AnimalCloning
• Although the clonedanimal willbe identical.It willonly
possess abouthalfthe life span of thenormalanimal
which has been cloned. An exampleis from the famous
‘Dolly’ previous mentioned whichonly lived for 6 years,
whereas normal sheep can live up to about10 years of
age, so a great decrease in age.
7. • Reasons for cloning aren’t exactly for keeping resources, such
as food for thefuture,but in fact we are basically taking
embryos from the animalfor research and by doing this leaving
theanimal useless. This is similar to humansbeing cloned,
where there humanembryos are takenaway from them for
research because they believe that it isn’t really a person.
However, if it is alive and has every trait thata humanshas,
thenhow can it be called useless to us?
8. • Even if we can clone animals and make them perfectly the same,
is this what God would’ve wanted? For us to have the power to
clone living organisms such as animals, and one day even human
beings?
• Many believe cloning is quite inhumane, especially that of
religious and some governmental parties which don’t want to
move forward with this research. They think life is just too
precious totake away, even if it is a clonein whichwe aretesting.
9. CLONED ANIMALS
In 1996, Scottish researchers shocked the world
with the news that they had cloned a sheep,
which they dubbed Dolly. Due to progressive
lung disease and arthritis uncommon for a
sheep of her age, Dolly was euthanized at age
6. (Her taxidermied remains are displayed at
The National Museum of Scotland, shown
here.) Dolly’s birth and death sparked a debate
about the ethics of animal cloning that
continues today. Some see cloning as the only
hope for certain, critically endangered species.
Here’s a look at some of the lesser-known
animals created through cloning. (Text:
Katherine Butler)
10. Indian bison, also known as gaur, look like a cross
between an ox and a water buffalo. They are
commonly found in Asian tropical woodlands in
places like Cambodia, Laos, China, India, Nepal and
Vietnam. As humansencroach on their wild
habitats, their numbers are dwindling. In 2001,
Bessie, an American cow, gave birth to a gaurclone
called Noah in Iowa. Noah initially exhibited
promise, and one of his creators told CNN that
"within 12 hours of birth, Noah was able to stand
unaided and began an inquisitive search of his new
surroundings." But just 48 hours after birth, Noah
succumbed to an intestinal disorder and died.
11. Theendangered European mouflon, also
known as a small, feral sheep, was first
cloned in 2001 in Italy. Threatened in its
original habitat of the Mediterranean islands
of Sardinia, Corsica and Cyprus, the
animal nearly diedout a century ago. The
mouflon was cloned using the same
technique scientists usedto createthe sheep
Dolly —a somatic cell nucleartransfer.
This is alab technique used to createan
ovum with a donor nucleus.
12. The domestic ferret was first cloned in
2006through somatic cell nuclear transfer, in
part to produce test subjects for human
medical research. However, the process may
be used to protect endangered ferrets as well.
The black-footed ferret is among the most
endangered mammalsin North America. A
recent boom in the prairie dog population,
which the ferret likes to eat, has slowly
brought their numbers back up. However, as
landowners often blame the ferret for
damagingcrops, their situation remains
tenuous.
13. Thewater buffalo, also known as the
Asian buffalo, is a large member of the
bovini family that hshorns that curve
backwardin a crescent shape andcan
grow to 6 feet tall. These animals enjoy
the muddy waters of tropical and
subtropical Asia, and they also forage on
aquatic plants and grasslands. They are
friends tohumans and have been
domesticated for at least 5,000 years. In
2005, the first water buffalo was clonedin
China in a study run by Guangxi
University.
14. Rhesus monkeys are what
National Geographic terms an
“old world animal,” astheir range
includes Afghanistan, Pakistan,
India, Southeast Asia and China.
Some introduced monkeys live in
the wilds of Florida. They are
social animalsthat live in female-
led communities featuring an
occasionaldominant male.
15. The banteng is a species of wild cattle found primarily in
Southeast Asia. Banteng, which are also known as native
Indonesian cattle, are listed by the World Conservation
Union as “severely threatened” as their numbers have
declined as much as 85 percent in the past 15 to 20 years.
A large herd of banteng resides in Australia, where they
are largely protected short of 40 males that hunters pay to
shoot each year. In an effort to preserve the species, two
banteng calves were born to surrogate cows in Iowa in
2003. The genetic material to clone the calves came from
the San Diego Zoo's Center for Reproduction of
Endangered Species, where genetic tissue from
endangered animals is being archived.
16. The African wildcat, which is found in Africa and the
Middle East, is a bit smaller than its domestic
counterpart. It is also one of the first wild species to
be cloned. The Audubon Center for Research of
Endangered Species announced in 2005 that their
cloned wildcats had bred and delivered two litters of
kittens. "By improving the cloning process and then
encouraging cloned animalsto breed and make
babies, we can revive the genes of individuals who
mightnot be reproductively viable otherwise, and we
can save genes from animalsin the wild,” Dr. Betsy
Dresser, who led the scientific team at the Audubon
Center, said in a BBC article.
17. The Pyrenean ibex was declared extinct
when the last of its kind was found dead in
its native Spain in 2000. But in 2009,
reports surfaced that scientists had
preserved DNA from the last known
Pyrenean ibex. Filling in the blanks with
DNA from domestic goats, a newborn ibex
was created, but died shortly after birth
due to lung problems. This was the first
time an extinct species was “resurrected,”
albeit for only a short time.
18. Itisnot just endangeredanimals thathavereceived the
attentionofscientists. The white-taileddeeris extremely
common in NorthAmerica. Nonetheless, researchers at
Texas A&M clonedthe first white-taileddeerin2003.
White-tailed deerare themost abundantbig-game
livestock inAmerica andranchers makeasignificant
amountofmoney from hunterswhopaytostalkthem
ontheirranches."Especially inthe stateof Texas, there
are alot ofranches thatmake moremoney on theirdeer
managementthanthey doontheirlivestock," researcher
MarkWesthusin, whohelpedcreate theclone, told
msnbc.com Westhusin alsosays thatcloning could
preserve some endangeredspecies of deer.
19. Idaho Gem
Theworld's first cloned mule, was
born on May 4. Heis an identical
genetic copy of his brother, a
champion racing mule called Taz,
and the first clone to be born in the
equine family.
20. Copycat
The world's first cloned kitten, named Cc.
It was created by scientists in Texas using
a cell taken from an adult tortoiseshell
female (see next picture). The photo,
taken on December 22 2001 when the
kitten was seven weeks old, was made
public in February 2002.