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RUAHA CATHOLIC UNIVERSITY
INSTITUTE OF ALLIED HEALTH SCIENCES
DERARTMENT OF MEDICAL LABORATORY SCIENCES(D.M.S)
COURSENAME:BASIC LABORATORYINSTRUMENTATION
COURSECODE: 04102
NATUREOF WORK:INDIVIDUAL ASSGNMENT
LECTURE NAME:MR. LUKA
STUDENT NAME:ANDERSON.E. LEONIDAS
REGISTRATION NUMBER:RU/CMLS/201 9/035
SUBMISSION DATE:29/11/2019
QUESTION:
1.Discuss on microscope filter
Microscope Filter:Is part of microscopeused to filt out unnecessary light but
also to increaseup the amount of light required.A variety of light filter is avariable
for use with the microscope.Light filter may be used to
.Increasing resolution and contrast
.decrease light intensity and glare
.Absorb excess heat
Microscopefilter are optical filters that are well suited for use in microscopeThey
are often placed over the illuminators and they commonly used in applications
such as fluorescence,brightfield, or darkfield microscopy.
๏ถ There are many types of filter used in microscopeand thoseare like the
following
๏ƒ˜ Neutral filters
๏ƒ˜ Coloured filters
๏ƒ˜ Heat-Absorbing filters
๏ƒ˜ Barrier filter(secondary filter)
๏ƒ˜ Exciter filters
๏ƒ˜ Colour Collection filters
๏ƒ˜ Exitation filters
NEUTRAL FILTERS
These are used to decrease the brilliance of the illuminant without
reducing the operating voltage of the bulb.They are manufactured in
number of densities,two or more of which may be used together to
obtain the effect of their combined strength.They are particularly useful
in photomicrography using colour film due to the close relationship
between the operating voltage and the colour temperature of the light
source.
EXITATION FILTERS
Is mainly and highly optical glass filters commonly used inflourescence
microscopy and spectroscope applications for selections of the
excitation wavelength of light from a light source.Also used to relatively
short wavelength from an excitation light source.
COLOURED FILTERS
These have a number of different functions โ€˜โ€™Day light blueโ€™โ€™is the most
commonly used filter in biomedical laboratory .Green filters similarly
increase resolution but also decrease ,increased contrast be obtained
with colourly filter by selecting one that is complementary to the colour
of the object under examination.
HEAT-ABSORBING FILTERS
These are used in conjuction with medium and high-intensity lamps
.Their purpose is to absorb the heat rays. They are important in high
resolution microscopy where the transfer of heat to the immersion oil
leas to a lowering of viscosity and consequent reduction in refractive
index which result in lower resolving power.
BARRIER FILTERS (secondary filters)
These are used to protect the retia from injury by preventing the
passage of ultraviolet light .They must,only transmit light of a longer
wavelength than the exciter filter.In addition ,they serve to increase the
brilliance of the fluorescent image by producing a dark background.
EXCITERFILTERS
These are used in fluorescence microscopy for transmitting light of
selected wavelength.
COLOUR CORRECTION FILTERS
These cover a very wide range .They are used mainly in colour
photomicrography in order to correct the colour temperature of the
light source to agree with that of the film.
Microscope filter are mostly used in different ways and therefore the
following are uses of microscope filter;
๏ƒ˜ To reduce the intensity of the light,when its requed example
ground glass light diffusing filter.
๏ƒ˜ To increase the contract and resolution example day light filter
and green filter increase the resolution.
๏ƒ˜ To transmit light of a selected wavelength example excitation
filter is used in fluorescence microscope .
๏ƒ˜ To protect the eye from injury caused by utrafluorescence
microscope.
Reference
๏ถ Monica cheesbrough-Medical laboratory manual for tropical
countries volume
๏ถ .J Baker ,R.E.Silveston introductionto medical Laboratory Technology ,7th
Edition,(2001) oxiford university Press
๏ถ Ruth Leventhal PHD,MBA,MT(ASP) Medical laboratory Technology
๏ถ RusselF. Cheadle ,MS,MT (ASP) Medical Laboratory Technology

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Anderson

  • 1. RUAHA CATHOLIC UNIVERSITY INSTITUTE OF ALLIED HEALTH SCIENCES DERARTMENT OF MEDICAL LABORATORY SCIENCES(D.M.S) COURSENAME:BASIC LABORATORYINSTRUMENTATION COURSECODE: 04102 NATUREOF WORK:INDIVIDUAL ASSGNMENT LECTURE NAME:MR. LUKA STUDENT NAME:ANDERSON.E. LEONIDAS REGISTRATION NUMBER:RU/CMLS/201 9/035 SUBMISSION DATE:29/11/2019 QUESTION: 1.Discuss on microscope filter
  • 2. Microscope Filter:Is part of microscopeused to filt out unnecessary light but also to increaseup the amount of light required.A variety of light filter is avariable for use with the microscope.Light filter may be used to .Increasing resolution and contrast .decrease light intensity and glare .Absorb excess heat Microscopefilter are optical filters that are well suited for use in microscopeThey are often placed over the illuminators and they commonly used in applications such as fluorescence,brightfield, or darkfield microscopy. ๏ถ There are many types of filter used in microscopeand thoseare like the following ๏ƒ˜ Neutral filters ๏ƒ˜ Coloured filters ๏ƒ˜ Heat-Absorbing filters ๏ƒ˜ Barrier filter(secondary filter) ๏ƒ˜ Exciter filters ๏ƒ˜ Colour Collection filters ๏ƒ˜ Exitation filters NEUTRAL FILTERS These are used to decrease the brilliance of the illuminant without reducing the operating voltage of the bulb.They are manufactured in number of densities,two or more of which may be used together to obtain the effect of their combined strength.They are particularly useful in photomicrography using colour film due to the close relationship between the operating voltage and the colour temperature of the light source.
  • 3. EXITATION FILTERS Is mainly and highly optical glass filters commonly used inflourescence microscopy and spectroscope applications for selections of the excitation wavelength of light from a light source.Also used to relatively short wavelength from an excitation light source. COLOURED FILTERS These have a number of different functions โ€˜โ€™Day light blueโ€™โ€™is the most commonly used filter in biomedical laboratory .Green filters similarly increase resolution but also decrease ,increased contrast be obtained with colourly filter by selecting one that is complementary to the colour of the object under examination. HEAT-ABSORBING FILTERS These are used in conjuction with medium and high-intensity lamps .Their purpose is to absorb the heat rays. They are important in high resolution microscopy where the transfer of heat to the immersion oil leas to a lowering of viscosity and consequent reduction in refractive index which result in lower resolving power. BARRIER FILTERS (secondary filters) These are used to protect the retia from injury by preventing the passage of ultraviolet light .They must,only transmit light of a longer wavelength than the exciter filter.In addition ,they serve to increase the brilliance of the fluorescent image by producing a dark background. EXCITERFILTERS These are used in fluorescence microscopy for transmitting light of selected wavelength.
  • 4. COLOUR CORRECTION FILTERS These cover a very wide range .They are used mainly in colour photomicrography in order to correct the colour temperature of the light source to agree with that of the film. Microscope filter are mostly used in different ways and therefore the following are uses of microscope filter; ๏ƒ˜ To reduce the intensity of the light,when its requed example ground glass light diffusing filter. ๏ƒ˜ To increase the contract and resolution example day light filter and green filter increase the resolution. ๏ƒ˜ To transmit light of a selected wavelength example excitation filter is used in fluorescence microscope . ๏ƒ˜ To protect the eye from injury caused by utrafluorescence microscope.
  • 5. Reference ๏ถ Monica cheesbrough-Medical laboratory manual for tropical countries volume ๏ถ .J Baker ,R.E.Silveston introductionto medical Laboratory Technology ,7th Edition,(2001) oxiford university Press ๏ถ Ruth Leventhal PHD,MBA,MT(ASP) Medical laboratory Technology ๏ถ RusselF. Cheadle ,MS,MT (ASP) Medical Laboratory Technology