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# Micro2microscopy

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### Micro2microscopy

1. 1. MICROSCOPY<br />
2. 2. USING THE METRIC SYSTEM TO EXPRESS THE<br /> SIZES OF MICROORGANISMS<br /> >The basic unit of length in the<br /> metric system, the meter (m), is<br /> equivalent to approximately 39.4 inches.<br />
3. 3. >A meter may be divided into 10 (101) <br /> equally spaced units called decimeters; or<br /> 100 (102) equally spaced units called<br />centimeters; or 1,000 (103) equally spaced<br /> units called millimeters; or 1 million (106)<br /> equally spaced units called micrometers; or<br /> 1 billion (109) equally spaced units called<br />nanometers.<br />
4. 4. MICROSCOPES<br /> >A microscope is an optical instrument <br /> that is used to observe tiny objects, often <br /> objects that cannot be seen at all with the<br /> unaided human eye.<br />
5. 5. Simple Microscopes<br /> >A simple microscope is defined as a <br /> microscope containing only one <br /> magnifying lens.<br />
6. 6. Compound Microscopes<br /> >A compound microscope is a microscope<br />that contains more than one magnifying<br /> lens.<br /> >Hans Jansen and his son Zacharias are often<br /> given credit for being the first to construct<br /> and use a compound microscope. <br />
7. 7. >Photographs taken through the lens system<br /> of compound microscopes are called<br /> photomicrographs.<br /> >The compound microscope is also referred<br /> to as a compound light microscope <br /> because visible light (from a built-in light<br /> bulb) is used as the source of illumination.<br />
8. 8. >Two magnifying lens systems:<br /> 1. Ocular lens- X10 magnifying power<br /> 2. Objective lenses- X4, X10, X40, & X100<br /> >Total magnification is calculated by<br /> multiplying the magnifying power of the<br /> ocular by the magnifying power of the<br /> objective being used.<br />
9. 9. >Resolving power is the distance between 2<br /> objects at which they cease to be seen as<br /> separate objects.<br /> >Objects can be examined with the <br /> compound light microscope that are as <br /> much as 1,000 times smaller than the<br /> smallest objects that can be seen with the<br /> unaided human eye.<br />
10. 10. >Other Types of Compound Microscopes:<br /> 1. Brightfield microscope– another name<br /> for the compound light microscope<br /> 2. Darkfield microscope- illuminated<br /> objects are seen against a dark <br /> background; used to diagnose syphilis<br /> 3. Phase-contrast microscope- used to<br /> observe unstained living microorganisms<br />
11. 11. 4. Fluorescence microscope- contains a<br /> built-in ultraviolet (UV) light source<br />
12. 12. Electron Microscopes<br /> - use an electron beam as a source of <br /> illumination and magnets to focus the beam<br /> > Two Types of Electron Microscopes:<br /> 1. Transmission electron microscope<br /> 2. Scanning electron microscope<br />