Advantages of ELISA | Dr.BGR Publications
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ELISA is an immunological plate-based assay technique that offers several advantages including high sensitivity and specificity due to the use of antibodies to detect antigens at the picogram level. ELISA also allows for high throughput using 96-well plate formats that can be adapted to 384-well plates. Additionally, ELISA protocols are easy to perform requiring little hands-on time and the assays are quantitative, cost-effective, and can test various sample types without the need for radioactive substances or large amounts of organic solvents.
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Advantages of ELISA | Dr.BGR Publications
- 3. introduction • it is an immunological assay Note Immunological assays / Immunoassay (IA): biochemical test
- 4. definition • it is a plate based assay technique
- 5. ADVANTAGES
- 6. it is common for ELISAS to detect Ag (antigens) at the picogram level in a very specific manner due to the use of Ab (antibodies). high sensitivity & specificity
- 7. high throughput commercial ELISA kits: 96-well plate format. but the assay can be easily adapted to 384-well plates.
- 8. easy to perform protocols are stress-free to follow & involve little hands-on time.
- 9. quantitative it can determine the concentration of Ag (antigen) in a sample
- 10. radioactive substances & large amounts of organic solvents are not required GENERALLY SAFE & ECO-FRIENDLY
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