Applications of ELISA | Dr.BGR Publications
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Enzyme-linked immunosorbent assay (ELISA) is an immunoassay used to measure antibodies, antigens, proteins, & glycoproteins in biological samples. ELISA is a plate-based technique.
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1) The document reviews various laboratory tests commonly used in public health surveillance such as microscopy, culture, antigen tests, PCR, and serology.
2) It discusses how to interpret laboratory test reports through specific examples and provides tips for maximizing the use of laboratory data, emphasizing the importance of understanding each test's strengths and limitations.
3) The document aims to simplify the interpretation of laboratory tests for public health professionals by defining key terms, explaining different testing methods and their pros and cons, and highlighting factors like specimen collection timing.
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Radioimmunoassay (RIA) is a sensitive technique introduced in 1960 to detect hormone levels in blood using antibodies and radioactive tracers. It represented the first invitro assay that could detect hormone levels and revolutionized research and clinical practice. RIA uses a radioactive label on the antigen or antibody to quantify its binding to antibodies or antigens, respectively, through competition. It allows for the detection of minute quantities of substances and is widely used in clinical diagnostics and research.
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ELISA applications.pdf
ELISA is a versatile laboratory technique used to detect and quantify specific substances in samples. It has applications in healthcare such as disease diagnosis for viral, bacterial, and parasitic infections as well as autoimmune diseases and allergies. ELISA is also used for hormone quantification, drug monitoring, cancer research, food safety by detecting allergens and pathogens, and environmental monitoring by detecting pollutants.
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2) It discusses how to interpret laboratory test reports through specific examples and provides tips for maximizing the use of laboratory data, emphasizing the importance of understanding each test's strengths and limitations.
3) The document aims to simplify the interpretation of laboratory tests for public health professionals by defining key terms, explaining different testing methods and their pros and cons, and highlighting factors like specimen collection timing.
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This document provides an introduction to radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), including their principles, instrumentation, procedures, applications, advantages and disadvantages. RIA uses radiolabeled substrates to measure trace amounts of antigens or antibodies, while ELISA uses enzyme-labeled substrates to avoid radiation hazards. Both methods rely on antigen-antibody binding and can be used to detect substances like hormones, drugs and proteins. However, RIA requires specialized equipment and handling of radioactive materials. ELISA has become more widely used as it provides sensitive, reproducible detection without radiation safety issues.
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Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
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Applications of ELISA | Dr.BGR Publications
- 3. introduction • it is an immunological assay Note Immunological assays / Immunoassay (IA): biochemical test
- 4. definition • it is a plate based technique • used to detect & quantify peptides, antibodies, proteins & hormones
- 5. applications
- 6. Detect & Measure the Presence of Antibodies in the blood Autoantibodies (ANTI-DSDNA, ANTI-DSG1, ANA, etc.) Antibodies against infectious disease (ANTIBACTERIAL, ANTIVIRAL, ANTIFUNGAL) Hepatitis A, B, C, HIV, etc
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