This study was conducted with an objective of adapting, see the yield, quality and recommend improved perennial grass accessions to the highland of Guji Zone, Ethiopia in a randomized complete block design (RCBD) with three blocks. Accessions were (Phalaris aquatica Sirossa Phalaris aquatica Sirocco, ILCA 14983, ILCA 14984 and variety X (Holeta local). Broadcasting method for Phalaris and 0.5meter between plant and 1meter between row spacing for Napier grass was applied at planting time each plot measuring 4meter x 6meter. Yield parameters were analyzed using statistical analysis software (SAS) and the means of quality parameters were compared with standards. Accordingly, Napier variety X was significant in survival rate, number of shoot, date of first harvest and harvesting frequency at (P<0.05) and highest in crude protein (CP) content. ILCA 14983 were also significant in survival rate and number of shoot and has highest in vitro dry matter digestibility (DOMD). Phalaris aquatica Sirossa was significant in green forage yield and has highest in vitro dry matter digestibility (DOMD). Generally, considering most parameters, Variety X and ILCA 14983 from Napier grass, Phalaris aquatica Sirossa from Phalaris were selected and recommended to the area and has to be evaluated under farmers’ conditions.

1. Adaptation Trial of Improved Perennial Grass Varieties/Accessions at the Highland of Guji zone, Bore, Ethiopia
IJPBCS
Adaptation Trial of Improved Perennial Grass
Varieties/Accessions at the Highland of Guji zone, Bore,
Ethiopia
Usman Semman1*, Tamirat Dinkale1 and Bedasa Eba2
1
Bore Agricultural Research Center, P.O. Box 21, Bore, Ethiopia
2
Yabello dry land and Agricultural Research Center. P.O. Box 085, Yabello, Ethiopia
This study was conducted with an objective of adapting, see the yield, quality and recommend
improved perennial grass accessions to the highland of Guji Zone, Ethiopia in a randomized
complete block design (RCBD) with three blocks. Accessions were (Phalaris aquatica Sirossa
Phalaris aquatica Sirocco, ILCA 14983, ILCA 14984 and variety X (Holeta local). Broadcasting
method for Phalaris and 0.5meter between plant and 1meter between row spacing for Napier grass
was applied at planting time each plot measuring 4meter x 6meter. Yield parameters were
analyzed using statistical analysis software (SAS) and the means of quality parameters were
compared with standards. Accordingly, Napier variety X was significant in survival rate, number
of shoot, date of first harvest and harvesting frequency at (P<0.05) and highest in crude protein
(CP) content. ILCA 14983 were also significant in survival rate and number of shoot and has
highest in vitro dry matter digestibility (DOMD). Phalaris aquatica Sirossa was significant in green
forage yield and has highest in vitro dry matter digestibility (DOMD). Generally, considering most
parameters, Variety X and ILCA 14983 from Napier grass, Phalaris aquatica Sirossa from Phalaris
were selected and recommended to the area and has to be evaluated under farmers’ conditions.
Key words: forage, Napier, perennial, survival, yield
INTRODUCTION
Despite the large number of livestock resources the
country own, its productivity is extremely low. The major
constraint to such low productivity is shortage of livestock
feeds in terms of quantity and quality, especially during the
dry season (Ahmed et al., 2010). Feed supply from natural
pasture fluctuates following seasonal dynamics of rainfall
(Solomon et al., 2008). Despite, these problems,
ruminants continue to depend primarily on forages from
natural pastures and crop residues.
There are grass forage varieties which are adapted to
different agro ecology of the country. These are divided as
annuals and perennials in life forms. Napier grass
(Pennisetium purpureum) and Phalaris are among
perennial grasses which are adapted to the high land of
country (Kediret al, 2007). Napier grass (Pennisetum
purpureum) has become by far the most important species
due to its wide ecological range of adaptation (from sea
level to over 2,000 meters), high yield and ease of
propagation and management (Orodho, 2006; ILRI,
2010a; ILRI, 2013). The grass is propagated vegetatively
by using stem cuttings, root splits or shoot tips which
usually vary across agro ecologies (Getnet and Gezahagn,
2012). This grass can provide a continual supply of green
forage throughout the year and best fits to all intensive
small scale farming systems (Alemayehu, 1997).
However, their yield performances vary across testing
environments.
*Corresponding Author: Usman Semman, Bore Agricultural
Research Center, P.O. Box 21, Bore, Ethiopia. Email:
usemman@yahoo.com, Cell phone: +251949761758, Fax:
+2514466670520
International Journal of Plant Breeding and Crop Science
Vol. 4(3), pp. 286-290, October, 2017. © www.premierpublishers.org. ISSN: XXXX-XXXX
Research Article

2. Adaptation Trial of Improved Perennial Grass Varieties/Accessions at the Highland of Guji zone, Bore, Ethiopia
Semman et al. 287
Removing or reducing nutritional constraints leads to
dramatic improvement in livestock production and
productivity. Dynamic changes have been made in the
areas of feed evaluation, nutrient requirement and feeding
systems (Seyoumet al., n.d). Several environmental,
genetic and genotype by environment interaction aspects
are expected to influence chemical composition and
nutritive value. Compositional data information on
digestibility and estimated metabolizable energy offers
opportunity to formulation of least cost ration.
There is no any animal feed technology which is tested in
the study area (Guji zone) and no information on forage
nutritional quality due to the remoteness of the area and
Bore Agricultural Research was established recently.
Therefore, this experiment was conducted with the
objectives of adapting, see the yield, quality and
recommend improved perennial grass (Phalaris and
Napier grass (Pennisetium Purpureum))
varieties/accessions to the area so as to address farmers
in high quality and large quantity of forages.
MATERIALS AND METHODS
Description of the Study Area
The experiment was carried out at Bore Agricultural
Research Center, which is one of the recently established
Research Centers of the Oromia Agricultural Research
Institute (OARI) in Bore district, Guji Zone of Oromia. Bore
district is located at 385 km to the south from Addis Ababa
and 220 km from the Guji Zone capital city (Negele) with
geographical location of 557'23” to 626'52” N latitudes and
3825'51” to 3856'21” E longitudes, South-eastern Oromia.
It has moist humid and sub humid moisture condition, with
relatively longer growing season. The annual rain fall is
about 1400-1800mm and the annual temperatures of the
district ranged from 10.1 to 20OC. The major soil types are
Nitosols (red basaltic soils) and Orthic Aerosols
(Yazachew and Kasahun, 2011).
Bore Agricultural Research station is located at 7km from
Bore district which is geographically located at 624'37” N
latitude and 3834'76” E longitudes. The research site
represents highlands of Guji Zone with an altitude of
2736m.a.s.l.receiving high rainfall characterized by
bimodal distribution. The first rainy season extends from
April to October and the second season starts late
November and ends at the beginning of March. The soil
type of the site is mostly black soil.
Experimental Design and Conduction of the
Experiment
The trial was arranged in randomized complete block
design (RCBD).There were three blocks each containing 2
plots of Phalaris accessions (Phalaris aquatica Sirossa
and Phalaris aquatica Sirocco) resulting to six plots in total
in 2010 summer season, 3 plots of Napier grass
accessions (ILCA14983, ILCA 14984 and variety X (Holeta
local)) resulting to nine plots in totalwith each plot
measuring 4m x 6min 2011 summer season. Distance
between plots and replication were 1m and 1.5m
respectively. Plots in each block were randomly assigned
to the each treatment. Broad casting method and 100kg
DAP and 100kg UREA per hectare were applied for
treatments of Phalaris aquatica Sirossa and Phalaris
aquatica Sirocco. Row method and 0.5m between plant
and 1m between row spacing (Tessema et al., 2002a)
were applied at planting time for treatments of Napier
grass (ILCA14983, ILCA 14984 and variety X (Holeta
local).
The nutrient analysis was conducted at Adami Tulu
Agricultural Research Center and Holeta Agricultural
Research Center Laboratory. Composite samples of
Napier grass (ILCA14983, ILCA 14984 and variety X
(Holeta local)) samples were taken at 1.5m height of the
forage. Composite samples of Phalaris (Phalaris aquatica
Sirossa and Phalaris aquatica Sirocco) were collected in
green stage at 50% flowering. Samples were dried at 65Oc
in a forced draft oven for 72 hrs. Samples were grounded
using a willy mill and allowed to pass through 1mm screen,
run in duplicates and Dry matter, Ash, CP, NDF, ADF, ADL
(lignin) and DOMD (In vitro dry matter Digestibility) were
determined by Near Infrared Reflectance (NIR) analysis
and Hemicellulose was calculated by subtracting the ADF
from the NDF content while cellulose was determined by
subtracting the ADL from the ADF content and data are
carefully collected.
Data Collected
Date of emergency, survival rate, number of shoot/plant,
plant height, date of first harvest, green forage yield and
harvesting frequency of Napier grass and date of
emergency, date of 50% flowering, date first harvest, plant
height and green forage yield of Phalaris and chemical
composition data were carefully collected for all
accessions/varieties at consecutive years.
Statistical Analysis
Data on agronomic parameters and yield was analyzed by
using SAS computer soft ware (SAS, 2002 version 9.1)
and GLM model was used at 5% significance level. Means
of chemical composition analysis were compared with
standards.
RESULT AND DISCUSSION
Yield and Yield Components
Napier grass (Pennisetum purpureum)
The analysis of variance (Table 1) shows that some
varieties have significant difference in survival rate,

3. Adaptation Trial of Improved Perennial Grass Varieties/Accessions at the Highland of Guji zone, Bore, Ethiopia
Int. J. Plant Breed. Crop Sci. 288
Table 1. Agronomic and yield parameters as influenced by Napier grass accessions/variety
Accessions/Variety Date of
emergency
Survival
rate
No of
shoot/plant
Plant height in
cm
Green forage
yield in ton/ha
Date first
harvest
Harvesting
frequency
ILCA 14983 18.00 65.87ab
23.12ab
113.33a
37.93a
205.00b
2.00b
ILCA 14984 18.00 47.62b
14.44b
66.44a
13.00a
215.00a
2.00b
Variety X 18.00 84.13a
32.44a
121.11a
77.20a
170.00c
4.00a
SEM 0 14.97371 8.076510 29.71176 44.37181 0 0
a, b
Means in a column within the same category having different superscripts differ (P<0.05);cm = Cent Meter; DM = Dry Matter; ha = hectare and SEM
= Standard Error of Means
Table 2. Agronomic and yield parameters as influenced by Phalaris accessions
Accessions Date of
emergency
Date of 50%
flowering
Date first
harvest
Plant height in cm Green forage yield tone/ha
Phalaris aquatica Sirocco 9.00 75.00 94.00 204.33 36.80b
Phalaris aquatica Sirossa 9.00 75.00 94.00 209.00 58.80a
SEM 0 0 0 12.33559 0
a, b
Means in a column within the same category having different superscripts differ (P<0.05);cm = Cent Meter; DM = Dry Matter; ha = hectare and SEM
= Standard Error of Means
Table 3.Chemical composition and digestibility as influenced by Napier grass accessions
Accessions DM % Ash OM CP NDF ADF ADL DOMD Cellulose Hemi-cellulose
ILCA 14983 93.18 9.05 84.12 3.79 70.16 50.27 9.49 53.42 40.78 19.89
ILCA 14984 93.80 8.50 85.33 3.90 70.12 48.23 8.78 52.41 39.45 21.89
Varity X 90.40 6.48 83.92 7.68 79.65 48.65 11.31 45.75 37.34 31.00
Mean 92.460 8.01 84.456 5.123 73.31 49.05 9.86 50.526 39.19 24.26
ADF=Acid Detergent Fiber; ADL=Acid Detergent Lignin; CP=Crude Protein; CV=Coefficient of Variation; DM=Dry matter; DOMD=In vitro dry matter
Digestibility; LSD=Least Significant difference; NDF=Neutral Detergent Fiber and OM=Organic Matter
number of shoot, date of first harvest and harvesting
frequency of Napier grass. Variety X was significant in
survival rate, number of shoot, harvesting frequency and
date of maturity than the rest accessions at (P< 0.05), but
at par with ILCA 14983 in survival rate and number of
shoot.
Phalaris
The analysis of variance (Table 2) shows that Phalaris
aquatica Sirossa was significant in green forage yield and
both are statistically the same in plant height.
Chemical composition
Napier grass (Pennisetum purpureum)
From the analyzed Napier grass (Table 3) variety X was
highest in Crude Protein (CP), Neutral Detergent Fiber
(NDF) and Acid Detergent Lignin (ADL) and less in Ash
and Dry matter Percentage (DM%)and Organic Matter
(OM) content, ILCA 14983 was highest in Ash, Acid
detergent Fiber (ADF) and In vitro dry Matter Digestibility
(DOMD) and less in CP content, ILCA 14984 was highest
in DM% and OM and less in NDF, ADF and ADL content
at (P<0.05).
Phalaris
The result of chemical analysis (Table 4) revealed that
Phalaris aquatica Sirocco was highest in OM, CP, NDF,
ADF and ADL content and Phalaris aquatica Sirossa was
highest in DOMD when compared to each other.
The CP content of all the tested Napier grass (Table 3)
accessions (ILCA 14983, ILCA 14984 and Variety X) was
below the average CP content of any feed (10.6) and the
CP content of young herbage to be as high as 14 to 16%
(Van Soest,1982). This level of CP is below the
recommended minimum level of CP in the diet of
ruminants for optimum rumen function (Van Soest,
1994).The result is also less than the CP content of
obtained by (Tessema, 2005) in north east Ethiopia which
was (12.2, 12.4 and 13.2) respectively. NDF content of all
accessions/varieties was greater than the average NDF
content of feed which is 56.2 and also higher than the
result obtained by (Tessema, 2005) which was (53.5, 54.7
58.2) respectively and the DOMD content of all
accessions/varieties was above the average DOMD
content of a feed (50.3) except Varity X and below energy
supplement feeds which is 82.2 and less than that of
obtained by (Tessema, 2005) which was (65.2, 64.7 57.5)
respectively. The difference is may be due to
environmental factors (soil and temperature), harvesting
stage, application of fertilizers and three years composite
sample of the previous study and method of analysis
employed. The CP content of all the tested Phalaris (Table
4) accessions was also less than the average CP content
of any feed (10.6) and the CP content of young herbage to
be as high as 14 to 16% (Van Soest, 1982).

4. Adaptation Trial of Improved Perennial Grass Varieties/Accessions at the Highland of Guji zone, Bore, Ethiopia
Semman et al. 289
Table 4.Chemical composition and digestibility as influenced by Phalaris accessions
Accessions DM% Ash OM CP NDF ADF ADL DOMD Cellulose Hemi-cellulose
Phalaris aquatica Sirocco 93.10 12.23 93.10 4.92 80.87 73.58 34.18 40.49 39.40 7.29
Phalaris aquatica Sirossa 92.82 10.63 82.19 4.27 72.83 38.58 30.93 59.83 7.65 34.25
Mean 92.96 11.43 87.65 4.59 74.87 56.08 32.55 50.16 23.53 18.79
ADF=Acid Detergent Fiber; ADL=Acid Detergent Lignin; CP=Crude Protein; CV=Coefficient of Variation; DM=Dry matter; DOMD=In vitro dry matter
Digestibility; LSD=Least Significant difference; NDF=Neutral Detergent Fiber and OM=Organic Matter
Roughage diets with NDF content of 45-65 and below 45%
were generally considered as medium and high quality
feeds, respectively (Singh and Oosting, 1992).However,
the NDF content of all accessions of Napier grass and
Phalaris was higher than 65% which classifies the feeds
under poor condition and ranged above the 66.2%
average value reported for tropical grasses (Van Soest,
1994). Therefore, some improvement mechanisms should
have to be employed. Roughages with less than 40% ADF
is categorized as high quality and those with greater than
40% as poor quality (Kellems and Church, 1998), and the
ADF value of Napier grass and Phalaris
accessions/varieties in the present study was greater than
40% except Phalaris aquatica Sirossa which was
(38.58%). This indicates that there has to be an
enhancement of the feeding value of the grass through
different agronomic techniques and application of fertilizer.
All accessions/varieties of Napier grass and Phalaris ADL
(limits DM intake) value was above 10% which is in a bad
range (Reed et al., 1986) except ILCA 14984 and ILCA
14983 which classifies the forage under good range of
quality. The cellulose content of the tested forages was
above the tropical grasses, which is 31.9% except Phalaris
aquatica Sirossa (7.93)and hemi-cellulose content of less
than tropical grasses, 35.4%, which classifies them as
good quality as noted by Moore and Hatfield (1994).
The DOMD value of all tested forage accessions/varieties
for the current study was in the range of the digestibility of
tropical grasses which lies between 50 to 60% (Own and
Jaysuria, 1989) which is considered to best quality except
for Napier Variety X (45.45) and Phalaris aquatica
Sirocco(40.49%).DOMD content of all the tested forages
with the exception of these two accessions/varieties were
above the average content of any feed which is 50.3%, but
less than the DOMD content of energy supplement feeds
which are 82.2%.The increase in digestibility also will lead
to increased feed intake as digestibility and feed intake are
positively correlated (Van Soest, 1982).
CONCLUSION
Access to new and improved agricultural technologies
especially that of forage is highly limited in Guji zone of
Oromia, most probably due to the remoteness from the
center and in accessibility of the area. That is why Bore
Agricultural Research Center paved the way to adapt and
recommend new accessions/varieties of forages to the
area. Based on the results obtained from the adaptation
and nutritional quality analysis improved perennial forage
grass accessions, Varity X and ILCA 14983 from Napier
grass, Phalaris aquaticaa Sirossa from Phalaris were
selected and recommended to the highland of the Guji
zone. Therefore, those accessions have to be evaluated
under farmers’ conditions so as to prove further their best
performing ability.
ACKNOWLEDGMENT
Thanks for Oromia Agricultural Research Institute (IQQO)
for financing the activity, Adami Tulu Agricultural Research
Center animal nutrition laboratory workers for the sample
preparation and Holeta Agricultural Research Center
animal nutrition laboratory workers for their analysis work.
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Accepted 18 August 2017
Citation: Semman U, Dinkale T, Eba B. (2017).
Adaptation Trial of Improved Perennial Grass
Varieties/Accessions at the Highland of Guji zone, Bore,
Ethiopia. International Journal of Plant Breeding and Crop
Science 4(3): 286-290.
Copyright: © 2017 Semman et al. This is an open-access
article distributed under the terms of the Creative
Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium,
provided the original author and source are cited.