The document describes a study that developed a mobile NMR lab to assess the physiological status of oilseed rape leaves grown in field conditions. A portable NMR spectrometer was placed in a van and used to measure the transverse relaxation times of leaf discs cut from plants in the field. The relaxation times detected structural changes during leaf senescence. Measurements on field-grown leaves were compared to those from controlled conditions to identify the effects of environmental variability on leaf tissues. The results demonstrated NMR can provide indicators of leaf development stage even for plants experiencing heterogeneous field conditions. This is an important step towards selecting genotypes tolerant to water and nutrient stress using non-destructive phenotyping methods.
Vadez et al. (2015) LeasyScan. J. Exp. Bot.-erv251CTom Hash
This paper describes the development of a new phenotyping platform called LeasyScan that combines 3D imaging and lysimetry to assess plant traits related to water use. LeasyScan uses a novel 3D scanning technique to continuously measure leaf area development of individual plants or plants grown at field densities. It also monitors plant transpiration through gravimetric measurements. Initial results found close agreement between scanned and observed leaf area. The paper discusses some early applications of LeasyScan including monitoring leaf development in pearl millet and assessing transpiration response to vapor pressure deficit in crops. This platform allows high-throughput phenotyping of important drought-related traits.
Treatment of municipal and industrial wastewater by reed bed technology: A lo...IJERA Editor
Reed bed system for wastewater treatment has been proven to be effective and sustainable alternative for conventional wastewater treatment technologies. Use of macrophytes to treat wastewater is also categorized in this method. This new approach is based on natural processes for the removal of different aquatic macrophytes such as floating, submerged and emergent. Macrophytes are assumed to be the main biological components of wetlands. These techniques are reported to be cost effective compared to other methods. Various contaminants like total suspended solids, dissolved solids, electrical conductivity, hardness, biochemical oxygen demand, chemical oxygen demand, dissolved oxygen, nitrogen, phosphorous, heavy metals, and other contaminants have been minimized using aquatic microphytes. In this paper, role of these plant species, origin and their occurrence, ecological factors and their efficiency in reduction of different water contaminants have been presented.
Effect of vegetation cover on sediment yield an empirical study through plots...Alexander Decker
This study examined the effect of vegetation cover on sediment yield through plot experiments in a lateritic environment in India. Five experimental plots with varying vegetation cover were monitored under natural rainfall conditions. Runoff and sediment yield were measured and compared between plots. Results showed that bare plots had higher sediment yields than vegetated plots. Statistical analysis revealed significant relationships between sediment detachment and explanatory variables like runoff volume and vegetation cover. Specifically, there was a very significant relationship between vegetation cover and sediment concentration. The plot-scale experiments provided detailed monitoring of the processes and demonstrated that increased vegetation cover reduces runoff and sediment yield in this lateritic environment.
“Performance of Tomato As Affected By Organic Mulching .pdfShynneGabinete
The document summarizes a study on the performance of tomato plants using different organic mulches. Specifically, it examines the effects of dried papaya leaves and dried banana leaves on tomato plant height and caudex diameter. Key findings include:
- Tomato plants mulched with dried papaya leaves grew taller on average than those mulched with dried banana leaves or the control group without mulch.
- While there were no statistically significant differences, tomato plants mulched with dried papaya leaves also had the widest average final caudex diameter.
- The study suggests dried papaya leaves may be a more effective organic mulch for tomato plants compared to dried banana leaves or no mulch.
Remote sensing is the acquisition of information about an object without physical contact. It is used in numerous fields including geography, earth sciences, and military applications. Remote sensing can be active, using emitted signals like radar, or passive, using reflected sunlight. Various techniques are used like radar, lidar, radiometers, and multispectral imaging from satellites and aircraft. These techniques can monitor vegetation condition, detect water stress, map land use, and more through indicators like chlorophyll content, water content, and leaf fluorescence. Both advantages like broad spatial coverage and disadvantages like need for ground truthing exist.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
The effect of organic mulch on the growth and yield of Spinach (Spinacia oler...Agriculture Journal IJOEAR
— An experiment was conducted in a greenhouse at the Luyengo campus of the University of Swaziland to determine the effect of organic mulch on the growth and yield of spinach. The experiment was conducted between the months of January and February, 2017. The experiment was a completely randomized design (CRD), with treated sewage mulch (TSM) and organic compost mulch (OCM) as treatments. The no mulch (NML) was used as a control. Spinach seedlings were planted at a spacing of 15 cm within rows and 30 cm between rows. Weeding and pest control measures were done uniformly across the treatments, when and as required. The data collected included the percentage moisture retained by the different treatments on the soil, growth parameters (leaf width, leaf length, leaf number, leaf area, plant height) and yield at harvest. The results displayed a significant difference (P<0.05)><0.05). The yield also varied across the treatments as TSM had a dry mass of 43.0 g, followed by OCM at 23.4 g and the no mulch treatment recorded a dry mass of 20.9 g. The results showed that mulching improved the performance of the spinach plants in terms of plant growth, moisture retention and yield.
This report summarizes a study of 10 bioretention sites installed as part of a stormwater management program. Soil samples from the sites were analyzed and found to generally meet specifications, though phosphorus levels were lower than specified. Vegetation assessments found varying levels of vigor and survivorship among plant species and sites. Proper coordination from design to construction to maintenance is important for bioretention system success, but lack of communication can lead to issues. The study aimed to understand how inconsistencies impact vegetation success.
Vadez et al. (2015) LeasyScan. J. Exp. Bot.-erv251CTom Hash
This paper describes the development of a new phenotyping platform called LeasyScan that combines 3D imaging and lysimetry to assess plant traits related to water use. LeasyScan uses a novel 3D scanning technique to continuously measure leaf area development of individual plants or plants grown at field densities. It also monitors plant transpiration through gravimetric measurements. Initial results found close agreement between scanned and observed leaf area. The paper discusses some early applications of LeasyScan including monitoring leaf development in pearl millet and assessing transpiration response to vapor pressure deficit in crops. This platform allows high-throughput phenotyping of important drought-related traits.
Treatment of municipal and industrial wastewater by reed bed technology: A lo...IJERA Editor
Reed bed system for wastewater treatment has been proven to be effective and sustainable alternative for conventional wastewater treatment technologies. Use of macrophytes to treat wastewater is also categorized in this method. This new approach is based on natural processes for the removal of different aquatic macrophytes such as floating, submerged and emergent. Macrophytes are assumed to be the main biological components of wetlands. These techniques are reported to be cost effective compared to other methods. Various contaminants like total suspended solids, dissolved solids, electrical conductivity, hardness, biochemical oxygen demand, chemical oxygen demand, dissolved oxygen, nitrogen, phosphorous, heavy metals, and other contaminants have been minimized using aquatic microphytes. In this paper, role of these plant species, origin and their occurrence, ecological factors and their efficiency in reduction of different water contaminants have been presented.
Effect of vegetation cover on sediment yield an empirical study through plots...Alexander Decker
This study examined the effect of vegetation cover on sediment yield through plot experiments in a lateritic environment in India. Five experimental plots with varying vegetation cover were monitored under natural rainfall conditions. Runoff and sediment yield were measured and compared between plots. Results showed that bare plots had higher sediment yields than vegetated plots. Statistical analysis revealed significant relationships between sediment detachment and explanatory variables like runoff volume and vegetation cover. Specifically, there was a very significant relationship between vegetation cover and sediment concentration. The plot-scale experiments provided detailed monitoring of the processes and demonstrated that increased vegetation cover reduces runoff and sediment yield in this lateritic environment.
“Performance of Tomato As Affected By Organic Mulching .pdfShynneGabinete
The document summarizes a study on the performance of tomato plants using different organic mulches. Specifically, it examines the effects of dried papaya leaves and dried banana leaves on tomato plant height and caudex diameter. Key findings include:
- Tomato plants mulched with dried papaya leaves grew taller on average than those mulched with dried banana leaves or the control group without mulch.
- While there were no statistically significant differences, tomato plants mulched with dried papaya leaves also had the widest average final caudex diameter.
- The study suggests dried papaya leaves may be a more effective organic mulch for tomato plants compared to dried banana leaves or no mulch.
Remote sensing is the acquisition of information about an object without physical contact. It is used in numerous fields including geography, earth sciences, and military applications. Remote sensing can be active, using emitted signals like radar, or passive, using reflected sunlight. Various techniques are used like radar, lidar, radiometers, and multispectral imaging from satellites and aircraft. These techniques can monitor vegetation condition, detect water stress, map land use, and more through indicators like chlorophyll content, water content, and leaf fluorescence. Both advantages like broad spatial coverage and disadvantages like need for ground truthing exist.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
The effect of organic mulch on the growth and yield of Spinach (Spinacia oler...Agriculture Journal IJOEAR
— An experiment was conducted in a greenhouse at the Luyengo campus of the University of Swaziland to determine the effect of organic mulch on the growth and yield of spinach. The experiment was conducted between the months of January and February, 2017. The experiment was a completely randomized design (CRD), with treated sewage mulch (TSM) and organic compost mulch (OCM) as treatments. The no mulch (NML) was used as a control. Spinach seedlings were planted at a spacing of 15 cm within rows and 30 cm between rows. Weeding and pest control measures were done uniformly across the treatments, when and as required. The data collected included the percentage moisture retained by the different treatments on the soil, growth parameters (leaf width, leaf length, leaf number, leaf area, plant height) and yield at harvest. The results displayed a significant difference (P<0.05)><0.05). The yield also varied across the treatments as TSM had a dry mass of 43.0 g, followed by OCM at 23.4 g and the no mulch treatment recorded a dry mass of 20.9 g. The results showed that mulching improved the performance of the spinach plants in terms of plant growth, moisture retention and yield.
This report summarizes a study of 10 bioretention sites installed as part of a stormwater management program. Soil samples from the sites were analyzed and found to generally meet specifications, though phosphorus levels were lower than specified. Vegetation assessments found varying levels of vigor and survivorship among plant species and sites. Proper coordination from design to construction to maintenance is important for bioretention system success, but lack of communication can lead to issues. The study aimed to understand how inconsistencies impact vegetation success.
Characterization of Diatraea saccharalis in Sugarcane (Saccharum officinarum)...Agriculture Journal IJOEAR
Abstract— Applications of remote sensing in agriculture have increased in recent years, especially for the development of sensors with better spatial and spectral resolutions. The objective of this study was to assess and evaluate the spatial and spectral variability of infection Diatraea saccharalis of sugarcane (Saccharum officinarum) through optical sensors in the Huasteca, Mexico. The methodology consisted in to make in situ measurements with a hyperspectral spectroradiometer in areas with and without apparent damage by the plague. For spatial and scaling representation Landsat 8 images were used. The data obtained in the field showed the spectral behavior of the plague; and the space-spectral reflectance variation was made by visibles and infrared bands for the vegetation. This process is an important approach to take a look from the geographical point of view to the problems related to the risk assessment of plague and diseases, their incidence, spread and severity, as well as support for sampling and monitoring activities. The used of these technologies provides advantages in research and in the implementation of precision farming techniques.
43rd Annual Symposium of the Ultrasonic Industry Association
http://us-biomat.com/events-media/congresses/43rd-annual-symposium-of-the-ultrasonic-industry-association/
M.D. Fariñas and T.E.G. Álvarez-Arenas
USBIOMAT, CSIC
Madrid,Spain
md.farinas@csic.es
t.gomez@csic.es
A method is presented for monitoring light/dark plant cycles based on the excitation and sensing of thickness resonances on the leaves, where the frequency-transmittance output curves show a clearly inverse relationship with the light intensity the plant receives. This is due to the variations induced in the plant activity (evapotranspiration) as response to variations in the light intensity. As a proof of concept, the technique has been applied to different plant types (monocots/dicots and deciduous/evergreen), different species, and genetically identical plants of the same species but grown under different environmental conditions.
This IA talks about research is to compare Simpson Diversity of four areas of Mahendrapur village based on the amount of sunlight received and the amount of nutrients found near the place where they are located (near the water body or away from the water body).
This document summarizes the development of a nanobiosensor to detect the herbicide diclofop. The nanobiosensor is based on atomic force microscopy (AFM) and uses the acetyl-CoA carboxylase (ACCase) enzyme, which is inhibited by diclofop, as the recognition element. Computational methods including molecular docking and molecular dynamics simulations were used to model the interactions between diclofop and ACCase and optimize the sensor design. Experimentally, ACCase was attached to AFM probes and force-distance curves were collected to measure the adhesion forces between the sensor and diclofop, demonstrating selective detection of diclofop over other herbicides. This
This document discusses methods of sampling vegetation. There are four main methods: transect, bisect, trisect, and quadrat. The transect method involves recording plant species along a line or belt and can show how plant communities change over a gradient. The bisect method examines the vertical layers of a plant community. The trisect method photographs a plot over time. The quadrat method uses rectangular or circular plots to measure plant coverage and abundance. The appropriate sampling method depends on the population, resources, and need for precision.
Human interactions with the environment are identified as one of the leading causes of climate change and variation. Modification, conversion and maintenance of land cover are all forms of anthropogenic interactions with the environment that result in a variety of vital changes to the environment that either positively or negatively feedback to the environment and climate. The identification and monitoring of these Land Use/ Land Cover Changes (LULCC) is therefore important since changes in land cover, occasioned more often than not by anthropogenic land use, alter land-atmosphere interactions upon which ecosystem services rely thus resulting in climate change and variation. Land Surface Temperature (LST) is a property of the land surface and refers to the temperature of the interface between the earth’s land surface and the atmosphere. It is therefore an important variable in land-atmosphere interactions and a climate change indicator which varies over space and in time as a function of vegetation cover, surface moisture, soil types, topography and meteorological conditions. Normalized Difference Vegetation Index (NDVI) is a numerical indicator derived from the Visible (Red) and Near Infrared (NIR) bands of the electromagnetic spectrum used in remote sensing to assess the concentration of green leaf vegetation and plant phenology. It is also an accepted and widely used parameter in characterization and assessment of vegetation change. This study uses a remote sensing approach in one of the most ecologically rich and diverse ecosystems to investigate the effect of Land Use/ Land Cover Change and in particular vegetation change on Land Surface Temperature (LST). The study area is in the Mara ecosystem located in South Western Kenya. LANDSAT satellite images for 1985, 1995, 2003 and 2010 were used to derive NDVI and LST. We found that human related Land Use/ Land Cover Change (LULCC) in the form of conversion of land for cultivation purposes has been and is taking place around the Maasai Mara National Reserve (MMNR). We also found that a negative correlation exists between LST and NDVI thus indicating that with decrease in vegetation cover and conversion to cultivated land, there is increase in Land Surface Temperature (LST).
- The study examined variation in hydraulic traits like xylem vessel diameter and specific leaf area (SLA) across populations of Protea repens (King protea) plants from different climate regions grown in a common garden experiment.
- Traits differed significantly among populations in the common garden, showing low phenotypic plasticity, with populations from drier areas having smaller vessel diameters and slower growth.
- All populations employed similar stomatal control and hydraulic strategies irrespective of source, regulating stress to avoid hydraulic failure during drought while maintaining photosynthesis and transpiration.
- This strategy is vulnerable to carbon starvation during prolonged drought, so populations with lower water stress thresholds may be more vulnerable to climate change impacts on water availability.
Evapotranspiration partitioning components in an irrigated winter wheat field...Agriculture Journal IJOEAR
Abstract— The arid and semi-arid regions constitute roughly one third of the total earth’s surface. In these regions water scarcity is one of the main limiting factors for economic growth. The impact of such water scarcity is amplified by inefficient irrigation practices, especially since about 85% of available water is used for irrigation in these regions. Therefore, a sound and efficient irrigation practice is an important step for achieving sustainable management of water resources in these regions. In this regard, a better understanding of the water balance is essential to explore water-saving techniques. In the context CRP project, experimental setups were conceived to monitor seasonal water consumption on the wheat crop irrigated by flood irrigation in Sidi Rahal station (middle of morocco. The partitioning of evapotranspiration compounds shows that transpiration dominates the evaporation about 68 % for three days (22, 23 and 24 February 2012). In addition the wheat absorbs the soil water from 10 cm to 20 cm (90%) at this growing stage according to the multiple-source mass balance assessment.
Evaluating efficacy of conceptual sediment barrier matrix towards ecosystem r...eSAT Journals
Abstract The rationale behind the study was to estimate effectiveness of Eichhornia leaf filter sleeves as a sediment barrier matrix towards
soil erosion and sediment control practice to curtail adverse impacts associated with sedimentation at the lake waters. The study
had an objective to implement the development to fit the site under study to stabilize exposed soils as soon as practicable. The
prime objective was to estimate the sediment captured by the sleeve to control and manage on-site by protecting the areas and
retain sediment on-site. Primarily Eichhornia leaf filter sleeves (Sun dried premature Eichhornia leaf fills ) crammed in an IS
16008 (2012)- tubular mesh sleeve as a linear land based treatment filter to primarily study the efficacy of filtration ability of
sediments and soluble pollutants a further contemplation. It is found that besides sediments some of the pollutants can be trapped
in sediments downstream. The efficacy of the model was analyzed to be successful in preventing sediment inflows at the lake
waters. Effectively percent reduction of sand 89%, TSS 68%, silt 56%, turbidity 51%, and clay by 18% was recorded. This
describes the effectual exploitation of the notorious weed Eichhornia biomass sleeve towards reducing sediments and turbidity of
runoff waters entering the lake waters enhancing the ecological status of the lakes. The studies portray the application of the
Eichhornia sleeve in high reduction in total suspended solids and sand.
Keywords: Conceptual Model, Sediment Barrier, Aquatic Ecosystem, Restoration.
This document summarizes a seminar presentation on high-throughput plant phenotyping. It discusses various imaging technologies used for plant phenotyping like 3D imaging, near infrared imaging, fluorescence imaging etc. It explains how these technologies are used to phenotype traits like growth, architecture, abiotic and biotic stress responses. The document also discusses the importance of phenotyping for plant breeding and genetics research. It highlights challenges in data management for large phenotyping datasets and the need for developing suitable analysis tools and sharing resources.
High throughput phenotyping are fully automated facilities in greenhouses or growth chambers with robotics, precise environmental control, and remote sensing techniques to assess plant growth and performance
This document requests proposals for developing a low-cost, portable in-situ sampler for microplastics in coastal environments. It summarizes a proposal submitted by Katherine Ball to create an angular backscatter detector called m-PARR (Micro-plastic Angular Refraction Recorder) that uses the principle of light refraction to identify microplastics pumped through the device. The proposal outlines developing m-PARR in stages through summer 2016, with field testing in September to demonstrate the device's ability to sample microplastics and engage citizen scientists. The deliverables would include a functional prototype, documentation, and a field testing report.
A Practical Guide For Academic Proposal WritingAmy Cernava
This document provides a detailed research proposal for a PhD project on using wavelet analysis to improve the accuracy of chlorophyll quantification in oil palm leaves through hyperspectral remote sensing. It includes background information on the significance of chlorophyll concentrations, an overview of the proposed methodology using wavelet transforms on reflectance spectra, research questions, and a Gantt chart outlining key milestones and a 126-week timeline. The goal is to develop a technique to generate accurate predictions of chlorophyll concentration and nitrogen content despite variations in other factors that influence leaf reflectance.
THE INFLUENCE OF GROWTH RETARDANTS AND SUBSTRATE VOLUME ON GROWTH OF HEMIGRAP...MandaManuela
Hemigraphis repanda L. Hallier is a colorful foliage plant with green and purple colored leaves with great potential for commercialization. This study was undertaken to determine if reduced nutrition space and plant growth regulators could limit excessive growth, while improving its overall quality. The H. repanda plants were exposed to four treatments: V1-6cm/water; V2-8cm/water; V3-10cm/water (Control); V4 -10 cm/Cycocel 0.2% (2000 ppm). The reduction of the substrate volume caused slower growth compared to the control plants (V3), the effects being comparable to those produced by the Cycocel treatments. Cycocel treated plants produced more lateral shoots resulting in a compact and dense bush-like appearance, which improved aesthetic value of Hemigraphis as a container-grown interior plant.
Effect of Watering Frequencies on the Growth and Survival of Petunia Axillari...Premier Publishers
Petunia axillaris is a very important economically cherished plant because of its ability to produce beautiful fragrant flowers that come in different colours, which invariably gives an aesthetic value. However, it is important to deepen the understanding for possible culture improvement. This study examined the effect of three watering frequencies on the growth and survival of Petunia from juveniles. The treatments consist of low water application (once in a week), normal application (twice per week) and high-water application (thrice per week). The following data were collected from the seedlings; plant height, number of flowers, number of dead plants. Also, destructive sampling was carried out to assess length and width of leaf, root depth, as well as number of leaves and number of dead leaves. Statistical analysis was performed with R-studio software. Growth and survival rate performances were significantly (p < 0.05) affected by watering frequencies. The multivariate analyses of principal component (PCA), showed that, all growth parameters except the number of dead leaves were correlated positively to each other. The results indicated that high flexibility of the species to be cultured with minimum requirements of water supply equating to reduction in production costs.
NANTOTECHNOLOGY IN AGRICULTURE AND FOOD TECHNOLOGYSaravananM957056
This document discusses various applications of nanotechnology in agriculture and food technology. It describes how silver nanoparticles, photocatalysis using metal oxides, and clay nanotubes can be used to improve plant growth and reduce pesticide use. It also discusses how nanosensors, electronic noses, nanobarcodes, carbon nanotubes, and mesoporous silica nanoparticles can enable precision agriculture through monitoring soil conditions, detecting chemicals and enabling targeted delivery of agricultural treatments. The overall aim of these nanotechnology applications is to increase crop yields while reducing costs, pesticide use, and environmental impacts.
The document discusses constructed wetlands for treating wastewater. It describes experiments conducted on constructed wetlands along Bilbeas Drain and Lake Manzala in Egypt. Pilot wetland systems were set up, including a surface flow system with cattail plants and a combined surface-subsurface flow system with cattail and water hyacinth plants. Water quality parameters were analyzed for the inflow and outflow of the wetlands. The experiments aimed to investigate the wetlands' ability to enhance drain water quality and the effects of different design parameters on treatment performance.
This document presents a dimensional analysis of the sludge filtration process using a sand drying bed. The analysis derives an equation to model sludge filtration that incorporates the compressibility coefficient. Experimental data from a pilot-scale sand drying bed is used to validate the theoretical equation. The experimental and theoretical slopes and intercepts show close agreement, with correlation coefficients ranging from 0.94-0.98, validating the derived equation. The equation accounts for factors such as sludge compressibility believed to affect the filtration process.
This document summarizes the application of a structure-index technique to evaluate soil structure degradation in clay soils in the Nile Delta region of Egypt. The technique involves treating soil aggregates with alcohol, water, and benzene to assess stability. Structure index values from 2-12 were observed across 14 soil profiles, with higher values indicating poorer structure. Structure degradation was found in bottom layers of half the profiles. Linear relationships were observed between structure index and electrical conductivity/sodium percentage, indicating early structure degradation with increasing salinity. The newly proposed index provides a simple way to rapidly assess soil structure changes across profiles and its relation to other soil properties.
This document describes the ARMETT technique, which stands for Arid Region Mapping of Evapo-Transpiration. The technique uses color infrared aerial photographs to differentiate between areas of actively transpiring vegetation and other land cover types in arid environments where water availability is the main limiting factor for plant growth. It does this through a filtering process called RERD (Red Edge Response Differentiation) which identifies pixels showing the red edge spectral response that indicates healthy, evaporating vegetation. When combined with knowledge of local plant rooting depths, this technique can be used to map the lateral extent of shallow groundwater resources. The document outlines the theory, methodology, testing and potential applications of the ARMETT technique.
Here are the steps to make a lace wig:
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My Teacher Essay Essay On My Teacher F. Online assignment writing service.Sherri Cost
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Characterization of Diatraea saccharalis in Sugarcane (Saccharum officinarum)...Agriculture Journal IJOEAR
Abstract— Applications of remote sensing in agriculture have increased in recent years, especially for the development of sensors with better spatial and spectral resolutions. The objective of this study was to assess and evaluate the spatial and spectral variability of infection Diatraea saccharalis of sugarcane (Saccharum officinarum) through optical sensors in the Huasteca, Mexico. The methodology consisted in to make in situ measurements with a hyperspectral spectroradiometer in areas with and without apparent damage by the plague. For spatial and scaling representation Landsat 8 images were used. The data obtained in the field showed the spectral behavior of the plague; and the space-spectral reflectance variation was made by visibles and infrared bands for the vegetation. This process is an important approach to take a look from the geographical point of view to the problems related to the risk assessment of plague and diseases, their incidence, spread and severity, as well as support for sampling and monitoring activities. The used of these technologies provides advantages in research and in the implementation of precision farming techniques.
43rd Annual Symposium of the Ultrasonic Industry Association
http://us-biomat.com/events-media/congresses/43rd-annual-symposium-of-the-ultrasonic-industry-association/
M.D. Fariñas and T.E.G. Álvarez-Arenas
USBIOMAT, CSIC
Madrid,Spain
md.farinas@csic.es
t.gomez@csic.es
A method is presented for monitoring light/dark plant cycles based on the excitation and sensing of thickness resonances on the leaves, where the frequency-transmittance output curves show a clearly inverse relationship with the light intensity the plant receives. This is due to the variations induced in the plant activity (evapotranspiration) as response to variations in the light intensity. As a proof of concept, the technique has been applied to different plant types (monocots/dicots and deciduous/evergreen), different species, and genetically identical plants of the same species but grown under different environmental conditions.
This IA talks about research is to compare Simpson Diversity of four areas of Mahendrapur village based on the amount of sunlight received and the amount of nutrients found near the place where they are located (near the water body or away from the water body).
This document summarizes the development of a nanobiosensor to detect the herbicide diclofop. The nanobiosensor is based on atomic force microscopy (AFM) and uses the acetyl-CoA carboxylase (ACCase) enzyme, which is inhibited by diclofop, as the recognition element. Computational methods including molecular docking and molecular dynamics simulations were used to model the interactions between diclofop and ACCase and optimize the sensor design. Experimentally, ACCase was attached to AFM probes and force-distance curves were collected to measure the adhesion forces between the sensor and diclofop, demonstrating selective detection of diclofop over other herbicides. This
This document discusses methods of sampling vegetation. There are four main methods: transect, bisect, trisect, and quadrat. The transect method involves recording plant species along a line or belt and can show how plant communities change over a gradient. The bisect method examines the vertical layers of a plant community. The trisect method photographs a plot over time. The quadrat method uses rectangular or circular plots to measure plant coverage and abundance. The appropriate sampling method depends on the population, resources, and need for precision.
Human interactions with the environment are identified as one of the leading causes of climate change and variation. Modification, conversion and maintenance of land cover are all forms of anthropogenic interactions with the environment that result in a variety of vital changes to the environment that either positively or negatively feedback to the environment and climate. The identification and monitoring of these Land Use/ Land Cover Changes (LULCC) is therefore important since changes in land cover, occasioned more often than not by anthropogenic land use, alter land-atmosphere interactions upon which ecosystem services rely thus resulting in climate change and variation. Land Surface Temperature (LST) is a property of the land surface and refers to the temperature of the interface between the earth’s land surface and the atmosphere. It is therefore an important variable in land-atmosphere interactions and a climate change indicator which varies over space and in time as a function of vegetation cover, surface moisture, soil types, topography and meteorological conditions. Normalized Difference Vegetation Index (NDVI) is a numerical indicator derived from the Visible (Red) and Near Infrared (NIR) bands of the electromagnetic spectrum used in remote sensing to assess the concentration of green leaf vegetation and plant phenology. It is also an accepted and widely used parameter in characterization and assessment of vegetation change. This study uses a remote sensing approach in one of the most ecologically rich and diverse ecosystems to investigate the effect of Land Use/ Land Cover Change and in particular vegetation change on Land Surface Temperature (LST). The study area is in the Mara ecosystem located in South Western Kenya. LANDSAT satellite images for 1985, 1995, 2003 and 2010 were used to derive NDVI and LST. We found that human related Land Use/ Land Cover Change (LULCC) in the form of conversion of land for cultivation purposes has been and is taking place around the Maasai Mara National Reserve (MMNR). We also found that a negative correlation exists between LST and NDVI thus indicating that with decrease in vegetation cover and conversion to cultivated land, there is increase in Land Surface Temperature (LST).
- The study examined variation in hydraulic traits like xylem vessel diameter and specific leaf area (SLA) across populations of Protea repens (King protea) plants from different climate regions grown in a common garden experiment.
- Traits differed significantly among populations in the common garden, showing low phenotypic plasticity, with populations from drier areas having smaller vessel diameters and slower growth.
- All populations employed similar stomatal control and hydraulic strategies irrespective of source, regulating stress to avoid hydraulic failure during drought while maintaining photosynthesis and transpiration.
- This strategy is vulnerable to carbon starvation during prolonged drought, so populations with lower water stress thresholds may be more vulnerable to climate change impacts on water availability.
Evapotranspiration partitioning components in an irrigated winter wheat field...Agriculture Journal IJOEAR
Abstract— The arid and semi-arid regions constitute roughly one third of the total earth’s surface. In these regions water scarcity is one of the main limiting factors for economic growth. The impact of such water scarcity is amplified by inefficient irrigation practices, especially since about 85% of available water is used for irrigation in these regions. Therefore, a sound and efficient irrigation practice is an important step for achieving sustainable management of water resources in these regions. In this regard, a better understanding of the water balance is essential to explore water-saving techniques. In the context CRP project, experimental setups were conceived to monitor seasonal water consumption on the wheat crop irrigated by flood irrigation in Sidi Rahal station (middle of morocco. The partitioning of evapotranspiration compounds shows that transpiration dominates the evaporation about 68 % for three days (22, 23 and 24 February 2012). In addition the wheat absorbs the soil water from 10 cm to 20 cm (90%) at this growing stage according to the multiple-source mass balance assessment.
Evaluating efficacy of conceptual sediment barrier matrix towards ecosystem r...eSAT Journals
Abstract The rationale behind the study was to estimate effectiveness of Eichhornia leaf filter sleeves as a sediment barrier matrix towards
soil erosion and sediment control practice to curtail adverse impacts associated with sedimentation at the lake waters. The study
had an objective to implement the development to fit the site under study to stabilize exposed soils as soon as practicable. The
prime objective was to estimate the sediment captured by the sleeve to control and manage on-site by protecting the areas and
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waters. Effectively percent reduction of sand 89%, TSS 68%, silt 56%, turbidity 51%, and clay by 18% was recorded. This
describes the effectual exploitation of the notorious weed Eichhornia biomass sleeve towards reducing sediments and turbidity of
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Keywords: Conceptual Model, Sediment Barrier, Aquatic Ecosystem, Restoration.
This document summarizes a seminar presentation on high-throughput plant phenotyping. It discusses various imaging technologies used for plant phenotyping like 3D imaging, near infrared imaging, fluorescence imaging etc. It explains how these technologies are used to phenotype traits like growth, architecture, abiotic and biotic stress responses. The document also discusses the importance of phenotyping for plant breeding and genetics research. It highlights challenges in data management for large phenotyping datasets and the need for developing suitable analysis tools and sharing resources.
High throughput phenotyping are fully automated facilities in greenhouses or growth chambers with robotics, precise environmental control, and remote sensing techniques to assess plant growth and performance
This document requests proposals for developing a low-cost, portable in-situ sampler for microplastics in coastal environments. It summarizes a proposal submitted by Katherine Ball to create an angular backscatter detector called m-PARR (Micro-plastic Angular Refraction Recorder) that uses the principle of light refraction to identify microplastics pumped through the device. The proposal outlines developing m-PARR in stages through summer 2016, with field testing in September to demonstrate the device's ability to sample microplastics and engage citizen scientists. The deliverables would include a functional prototype, documentation, and a field testing report.
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Effect of Watering Frequencies on the Growth and Survival of Petunia Axillari...Premier Publishers
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This document discusses various applications of nanotechnology in agriculture and food technology. It describes how silver nanoparticles, photocatalysis using metal oxides, and clay nanotubes can be used to improve plant growth and reduce pesticide use. It also discusses how nanosensors, electronic noses, nanobarcodes, carbon nanotubes, and mesoporous silica nanoparticles can enable precision agriculture through monitoring soil conditions, detecting chemicals and enabling targeted delivery of agricultural treatments. The overall aim of these nanotechnology applications is to increase crop yields while reducing costs, pesticide use, and environmental impacts.
The document discusses constructed wetlands for treating wastewater. It describes experiments conducted on constructed wetlands along Bilbeas Drain and Lake Manzala in Egypt. Pilot wetland systems were set up, including a surface flow system with cattail plants and a combined surface-subsurface flow system with cattail and water hyacinth plants. Water quality parameters were analyzed for the inflow and outflow of the wetlands. The experiments aimed to investigate the wetlands' ability to enhance drain water quality and the effects of different design parameters on treatment performance.
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How to Add Chatter in the odoo 17 ERP ModuleCeline George
In Odoo, the chatter is like a chat tool that helps you work together on records. You can leave notes and track things, making it easier to talk with your team and partners. Inside chatter, all communication history, activity, and changes will be displayed.
This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
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In Odoo, making a field required can be done through both Python code and XML views. When you set the required attribute to True in Python code, it makes the field required across all views where it's used. Conversely, when you set the required attribute in XML views, it makes the field required only in the context of that particular view.
How to Manage Your Lost Opportunities in Odoo 17 CRMCeline George
Odoo 17 CRM allows us to track why we lose sales opportunities with "Lost Reasons." This helps analyze our sales process and identify areas for improvement. Here's how to configure lost reasons in Odoo 17 CRM
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPRAHUL
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
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Remote Sensing and Geographic Information Systems
9
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Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
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2. Page 2 of 11
Musse et al. Plant Methods (2017) 13:53
assessment of leaf chlorophyll by Multiplex [4] and
Dualex [5] have been used for outdoor characterization
of whole plant N status and leaf development. On the
other hand, the use of leaf ranking or leaf ageing as indi-
cators of developmental status is not relevant for geno-
type comparison when environmental conditions (light,
temperature, wind, canopy structure, etc.) are responsi-
ble for marked heterogeneity among individual plants.
The main problem with the methods cited above, and
with more classical approaches like gas exchange meas-
urements [6], is that they do not detect small differences
in physiological traits between successive leaf ranks
throughout the canopy.
The potential of nuclear magnetic resonance (NMR)
relaxometry to finely evaluate the cell and tissue struc-
ture of oilseed rape leaves was recently demonstrated
[7–9]. The NMR transverse relaxation time (T2), which is
particularly sensitive to variations in water properties in
plant tissues, was used to study changes in cell water sta-
tus and distribution. As demonstrated in different plant
tissues including leaves, differences in the physical and
chemical properties of water in different compartments
and the relatively slow diffusion exchange of water mol-
ecules between compartments are reflected by the multi-
exponential relaxation times [9, 10]. Applied to a wide
panel of leaves collected from oilseed rape plants of dif-
ferent genotypes grown in controlled conditions, NMR
relaxometry was shown to be able to detect slight vari-
ations in senescence associated structural modifications
in leaf tissues [7–9]. This characterization of the internal
structure of the leaf allows accurate determination of leaf
development stage independently of its position along
the plant. In the context of fine phenotyping at individ-
ual plant scale in field conditions, the ability of NMR to
identify leaves at the equivalent developmental stage and
hence to allow plant traits to be compared is of particu-
lar interest. Moreover, NMR makes it possible to monitor
changes in water exchanges and structural changes asso-
ciated with remobilization efficiency [7–9].
Until now, the great majority of NMR and magnetic
resonance imaging (MRI) studies on plants have been
performed under controlled conditions in the labora-
tory. Current trends in the further development of the
NMR/MRI method, largely motivated by phenotyping
needs, are to extend investigations to in situ experiments
(climate chambers, greenhouses or the natural environ-
ment) rather than to transport plants to the laboratory
where the equipment is located. Relatively recent impor-
tant technological advancements have been reported in
mobile NMR devices. A single-sided open NMR sen-
sor equipped with a permanent magnet for near surface
studies, which allows free access to large objects, known
as NMR MOUSE®
[11], was developed for a range of
applications [12, 13], such as the characterization of soils,
mortars and painting, as a logging tool for the petroleum
industry, etc. It has also been used to study leaf water sta-
tus in situ [14] and to determine the moisture fraction in
wood [15]. Another approach has been to design specific
NMR and/or MRI devices that can easily been placed or
transported into climate chamber, greenhouse or in the
field. For example, a small device known as NMR-CUFF
[16] with a modified Halbach-type magnet that can be
opened for sample positioning was developed by Windt
et al. [16] and used to measure sap flow and the amount
of water in plants in a climate chamber [17]. Another
example of such designed instruments is the NMR sys-
tem developed by Van As et al. [18] equipped with a per-
manent U-shaped magnet, with an access space of 2 cm
used to study water content and transport in plants in
greenhouses and climate chambers [19, 20]. Recently, a
size-adjustable radiofrequency coil allowing investiga-
tion of plant samples of different diameters in a Halbach
magnet has been proposed [21]. Finally, a few portable
MRI systems have recently been developed mostly for the
imaging of relatively small living trees for use in green-
houses [17, 22–24].
Only a limited number of NMR/MRI studies have been
performed on plants in their natural environment. Capi-
tani et al. used NMR relaxometry to investigate the water
status of rockrose and holm oak leaves growing in sand
dunes [14]. Okada et al. [25] reported the first outdoor
MRI imaging of a living tree using a 0.3 T permanent
magnet. A few years later, a permanent magnet equipped
with flexible rotation and translation mechanism and
combined with a mobile lift was used for outdoor imag-
ing of pear tree branches up to 2 cm in diameter and
up to 160 cm above ground level [26]. Jones et al. [27]
designed a transportable MRI system offering an access
space of 21 cm diameter for the imaging of living trees in
the forest. Geya et al. [28] built a mobile MRI system with
a 16 cm gap 0.2 T permanent magnet for measurements
of the relaxation times and apparent diffusion coeffi-
cients of pear fruits in an orchard. This MRI system was
recently shown to be able to measure water transport in
trees outdoors [29].
The development of outdoor NMR and MRI measure-
ments has faced two major challenges. The first concerns
the NMR/MRI device itself and the effects of the envi-
ronmental conditions on it. The system needs to be port-
able and easy to handle in different conditions. Further,
the temperature drift of the magnet, the lack of homoge-
neity of the magnetic field and the variations in sample
temperature can make it difficult to distinguish variations
in the signal due to the biological changes under study
from variations due to the system or measurement condi-
tions. Some applications require accurate measurements
3. Page 3 of 11
Musse et al. Plant Methods (2017) 13:53
and exploitation of the complete NMR signal. For exam-
ple, in the specific case of characterization of the pro-
gress of senescence in oilseed rape leaves based on slight
variations in multi-exponential relaxation parameters [8,
9], small variations in sample or magnet temperature can
alter the results. Given the amplitude of possible varia-
tions in temperature due to weather conditions in the
field, it is clear that the temperature of both the magnet
and the sample have to be controlled. Furthermore, the
inhomogeneous field, like that of the unilateral portable
NMR system, is an additional source of relaxation, which
shortens the T2 values measured [14]. The second chal-
lenge facing the study of plants grown in field conditions
is related to the biological variability of the plant material.
There are two potential sources of random variations in
plant tissue characteristics that can alter the NMR signal.
The main source is associated with the abiotic and biotic
factors experienced by the plant throughout its develop-
ment that lead to hardening of the leaf tissues. Further,
compared to control conditions, plants grown in the field
present higher variability in their canopy architecture,
which is responsible for additional micro-environmental
differences immediately prior to sampling. These aspects
can cause erroneous results if the NMR measurements
are simultaneously polluted by instabilities of the NMR
device. The solution is to perform NMR measurements
in the field using an NMR device that has been care-
fully checked in controlled laboratory conditions and
to ensure the same measurement accuracy. Moreover, if
field measurements are compared with those obtained on
plants grown under well-controlled conditions, it is pos-
sible to identify the specific effects of variabilities caused
by environmental factors.
The objective of this study was to demonstrate the
potential of NMR to access information about the sta-
tus and sub-cellular distribution of water in leaves from
plants grown in natural conditions, thereby providing
robust indicators of the stage of development directly in
the field. To this end, a mobile NMR lab was designed
for in situ measurements of the relaxation times of
leaves from plants in their natural environment. A com-
mercially available NMR spectrometer similar to that
previously used for investigations of oilseed rape leaves
[7–9] was used to create a mobile laboratory with the
same performance as in the well-controlled laboratory
experiments. The device was designed to be positioned at
the edge of individual parcels in a field trial. The meas-
urements made on the leaves of plants grown and ana-
lyzed in the field were compared to the measurements
on plants grown in controlled conditions and analyzed
in the laboratory. Results of the comparison showed that
NMR can detect structural modifications in leaf tissues
associated with senescence progress despite plant hetero-
geneity found under natural conditions.
Methods
NMR relaxometry
Instrumental setup
Transverse relaxation measurements were performed
using a mobile NMR lab specifically set up for this
purpose (Fig. 1). A commercially available 20 MHz
spectrometer (Minispec PC-120, Bruker, Karlsruhe, Ger-
many) equipped with a temperature control device con-
nected to an optical fiber (Neoptix Inc, Canada) allowing
±0.1 °C temperature regulation was placed inside a van.
The experimental device was powered by a battery. Such
equipped van was positioned in the field close to the
plants under investigation. No special care was taken to
control the temperature inside the van. The leaf under
investigation was cut from the plant (Fig. 2a) without
uprooting the plant and, if the leaf was wet, wiped gen-
tly. Eight discs 8 mm in diameter were cut from each leaf
of the plant studied (Figs. 2b, c). To obtain homogeneous
tissues, the discs were cut in the middle of the limb on
each side of the central vein and avoiding lateral second
order veins. The discs were then placed in NMR tubes
which were closed with a 2-cm long Teflon cap to avoid
water loss during measurements (Fig. 2d). The tempera-
ture of the samples inside NMR probe was set at 18 °C.
Transverse relaxation signal acquisition and analysis
The transverse relaxation times was measured using
the Carr–Purcell–Meiboom–Gill (CPMG) sequence.
The signal was acquired with the 90°–180° pulse spac-
ing of 0.2 ms. Data were averaged over 64 acquisitions.
The number of successive echoes recorded was adjusted
Fig. 1 Mobile NMR laboratory with the NMR spectrometer including
the magnet system and the probe assembly (1), the electronic control
NMR unit (2), the battery (3) and a standard laptop computer for
measurement control (4)
4. Page 4 of 11
Musse et al. Plant Methods (2017) 13:53
for each sample according to its T2. The recycle delay
for each sample was adjusted after measurement of the
longitudinal relaxation time (T1) with a fast-satura-
tion-recovery sequence. The measurement time for T2
(including spectrometer adjustments and T1 measure-
ment) was about 10 min per sample.
The CPMG signal was fitted using Scilab software
according to the maximum entropy method (MEM) [30],
which provides a continuous distribution of relaxation
time components with no assumption concerning their
number. In this representation, the peaks of the distribu-
tion are centered at the corresponding most probable T2
values, while peak areas correspond to the intensity of the
T2 components. Signal intensity was expressed through
the specific leaf water weight of the ith
signal component
(LWW) expressed in g m−2
. LWW of each CPMG com-
ponent was calculated according to the equation:
where I0Ri is the relative intensity of the ith signal compo-
nent expressed as a percentage of the total CPMG signal
(1)
LWWi =
I0Ri × mw
A
intensity, mw is the water mass of the leaf discs used for
NMR (in g) and A is the leaf disc area (in m2
).
Plant material
Oilseed rape (Brassica napus L., genotype Aviso)
plants were grown in a field trial in Le Rheu, France
(La Gruche, 48°8′17″N–1°48′11″O) during the 2014–
2015 cropping season. The seeds were sown on the
10th of September, 2014 in plots measuring 6.75 m2
(4.5 × 1.5 m, 4 rows) at a density of 45 seeds m−2
and
were grown under an optimal N regime. Four plots were
used for this experiment corresponding to four repeti-
tions. Measurements were made between the 23rd and
the 26th of March 2015, at the end of the stem elonga-
tion stage (Fig. 2a), while floral buds were still closed
(BBCH 55), on all fully expanded leaves (about 17 leaf
ranks) of one individual plant (in four different plots,
corresponding to four replicates).
Indicators of leaf physiological status
Chlorophyll content
Before sampling the leaf discs for the NMR experiment,
relative chlorophyll content per unit leaf area was esti-
mated using a non-destructive chlorophyll meter (SPAD,
Soil Plant Analysis Development; Minolta, model SPAD-
502). The chlorophyll content of each leaf was estimated
as the average of six independent measurements.
Water content
Leaf discs were kept in the closed NMR tubes until the
end of the NMR experiment each day. The samples were
transferred in the laboratory and water content was then
determined by weighing before (fresh weight) and after
drying (dry weight) in an oven at 70 °C for 48 h. Water
content is expressed as a percentage of fresh weight.
Comparison of the outdoor and laboratory NMR
measurements
The outdoor NMR measurements made in the present
study were compared with the NMR measurements per-
formed on plants grown under controlled conditions
and analyzed in the laboratory (data from [7, 8]). The
objective of this comparison was to evaluate the NMR
parameters as indicators of the leaf development stage in
plants grown and analyzed in outdoor conditions. Data
obtained on leaves from two different sets of plant were
used for the comparison:
1. 32 non-vernalized oilseed rape plants of Tenor geno-
type, details are reported in [8]
2. 20 vernalized oilseed rape plants of Aviso genotype,
details are reported in [7]
Fig. 2 Oilseed rape plants at the end of stem elongation stage (a),
leaf (LR −9) after disc sampling (b), eight discs cut from the leaf for
NMR measurement (c) and NMR tube containing 8 discs, closed with
a 2-cm long Teflon cap (d)
5. Page 5 of 11
Musse et al. Plant Methods (2017) 13:53
Like in the outdoor experiment, the NMR device used
for transverse relaxation times in controlled conditions
was a 20 MHz spectrometer (Minispec PC-120, Bruker,
Karlsruhe, Germany). The CPMG measurements were
performed at 18 °C with the 90°–180° pulse spacing of
0.1 ms and 64 signal averages.
Both NMR results and parameters describing physi-
ological status measured were represented according to
the NMR split scale. It was previously shown [8] that it
is possible to use the split of the longest T2 signal com-
ponent measured in the mature leaves to target leaves
at the same developmental stage. In this representation,
leaf rank zero is assigned to the leaf of the last leaf rank
in which the split occurred, the subsequent leaf rank is
numbered 1, etc. According to this scale, the older the
leaf, the higher its rank, while negative ranks represent
young leaves in which split has not yet occurred. This
NMR split scale makes it possible to average values from
data obtained in leaves located at different positions in
the canopy. The NMR split scale is used in all the follow-
ing figures.
Results
NMR field measurements
Two typical examples (plants 1 and 2) of the transverse
relaxation time distribution for two leaf development
stages (leaf ranks −2 and 0) are shown in Fig. 3. For each
stage, the curves are compared with the representative
transverse relaxation spectrum obtained on leaves from
plants grown under controlled conditions [7] at the same
developmental stage according to the split scale. For
the youngest leaves (leaf rank −2) from the field experi-
ment, the longest T2 component corresponding to the
vacuolar water was centered at 150–200 ms, which cor-
respond to slightly higher values than those measured
in the controlled conditions (Fig. 3a). This component
corresponded to the largest amount of leaf water as
it was observed in the plants grown under controlled
conditions. For the older leaves (leaf rank 0), the vacu-
olar signal split into two components. The T2 values of
the longest T2 components of the two spectra depicted
in Fig. 3c were very different, illustrating the high vari-
ability of this parameter in senescing leaves, as already
Fig. 3 Distribution of transverse relaxation time (T2) calculated from the CPMG data for different leaves from plants grown in the field compared
with the results obtained under controlled conditions from [7] Sorin et al., Botanical Studies 2016, 57; acknowledgment to Springer. a corresponds
to the leaf rank −2 with b the zoom of the T2 distribution up to 30 ms. c corresponds to the leaf rank 0 with d the zoom of the T2 distribution up to
30 ms
6. Page 6 of 11
Musse et al. Plant Methods (2017) 13:53
reported in [8, 9] and attributed to the high rate of struc-
tural changes responsible for the variations in the signal.
In addition to the vacuolar component described
above, under controlled conditions, two other CPMG
components were systematically observed. Figure 3b,
d are zooms up to 30 ms on the T2 spectra shown in (a)
and (c), respectively. The first one was centered at a few
ms and represented a small percent of the water amount.
It was always observed in the youngest leaves (leaf rank
−2), disappeared with leaf age and was consequently
observed only in some leaves of leaf rank 0. The second
component was centered at about 15 ms and represented
less than 20% of the water amount for all leaf ranks.
Under field conditions, the signal differed from what was
expected according to these results. Actually, the number
of components detected in the T2 range 0–30 ms varied
between one and three. In the case of two components
(more than 50% of the leaves analyzed), the same gen-
eral frame was found as that observed under controlled
conditions (Fig. 3b leaf rank −2, plant 1 and d leaf rank
0 plant 2). In the case of three components detected
(about 10% of the leaves), the additional component was
detected with an intermediate T2 value of 5–8 ms (Fig. 3b
leaf rank 0, plant 1). Finally, in the case of one component
detected, the T2 value of this component was centered at
5–8 ms. Note that in all cases, the sum of the intensities
of all these components represented approximately the
same percentage of the total signal for a given leaf rank.
Figure 4 shows the T2 (a) and LWW (b) values of the
NMR signal components (4 and 5) associated with the
vacuole during leaf ageing in plants grown in the field.
Data from plants grown in controlled environment con-
ditions extracted from [7, 8] are shown on the same
graphs for the purpose of comparison. All the data are
plotted according to the NMR split scale leaf rank (see
“Methods”). In the case of the component 4, T2 values
were reproducible over the whole leaf rank scale in all
three growing conditions (Fig. 4a). After leaf rank 0, the
maximum rate of the increase in T2(5) was the same in
the different experiments (about 200 ms per leaf rank).
However, while this maximum rate followed the appear-
ance of the fifth component in plants grown in field con-
ditions, it was delated for leaves from [8] where T2(5)
increased at the maximum rate between leaf ranks +5
and +7.
Note that in the case of the field measurements, it was
possible to analyze leaves characterized by leaf ranks up
to −15, corresponding to very young leaves. This made
it possible to confirm the increasing trend in the value
of T2(4) with ageing of the leaf. Considering differences
in genotypes, vernalization and environmental factors
between plant growing conditions, Fig. 4a shows that
it was possible to establish a master curve describing
the structural changes in young leaves (negative leaf
ranks). After LR = 0, T2 values describing structural leaf
changes appeared to be more affected by environmental
conditions.
According to [7–9], the NMR intensities of each signal
component (Fig. 4b) are expressed in leaf water weight
(Eq. 1). Like in the plants grown under controlled con-
ditions, LWW (4) increased steadily until leaf rank 0,
reflecting the progressive increase in the amount of vacu-
olar water with aging. An unexpected result was that for
the positive leaf ranks associated with the four oldest
leaves, LWW (4 + 5) corresponding to vacuolar water
Fig. 4 Transverse relaxation time (a) and specific leaf water weight
(b) corresponding to vacuolar water of leaves during leaf develop-
ment according to the NMR split scale leaf rank. The results of the
field experiment are compared with the results obtained under
controlled conditions [7]—Sorin et al., Botanical Studies 2016, 57;
acknowledgment to Springer; [8]—Sorin et al., Planta. 2015, 24;
copyright Springer. As the leaves were rearranged according to the
NMR split scale after sampling, in the case of the field experiment the
values corresponding to the leaf ranks between 0 and −10 are the
averages ± standard deviations of data collected from leaves of four
plants, for leaf rank −11 the data is the average ± standard deviations
of data collected from leaves of three plants. For leaf ranks between
−15 and −12 and between 3 and 1 less than tree measurements
were available for analysis
7. Page 7 of 11
Musse et al. Plant Methods (2017) 13:53
decreased in the field experiment in contrast to the data
reported in [7, 8].
Physiological characterization of leaf development
Changes in physiological traits during leaf development
were described through general parameters (Fig. 5)
i.e. chlorophyll content, dry weight and water content.
These data, like those presented in Fig. 4 are presented
according to the NMR split scale and compared with
data obtained on plants grown in controlled environ-
ment conditions extracted from [7, 8]. The chlorophyll
content measured in the field was at its maximum value
from leaf rank −13 to −4 and decreased markedly from
leaf rank −2 (Fig. 5a) to a very low value for the oldest
leaves. These results show that our study was performed
on a large panel representing a relatively wide range of
young, mature and senescent leaves. In controlled con-
ditions, the same general trend was observed. However,
the curve representing chlorophyll content measured
on non-vernalized plants [8] started to decrease for LR
1, which corresponded to older leaves, compared to the
curves obtained on vernalized plants independently
of the trial. In field conditions, the dry weight (Fig. 5b)
increased slightly from leaf rank −15 to −2, reflecting
the production of biomass associated with leaf growth.
It then dropped from leaf rank −1, reflecting the loss of
about 75% of leaf biomass explained by major remobiliza-
tion at leaf senescence. This was consistent with the data
obtained in controlled conditions [7, 8]. Note that in non-
vernalized plants, specific dry weight had lower values
than in vernalized plants. In field conditions, leaf water
content increased slightly from the youngest leaves to
leaf rank −3, indicating that leaf tissue was able to main-
tain cell homeostasis. From leaf rank −4, the loss of dry
matter was more marked than that of water, resulting in
an increase in water content. The same general trend was
observed in controlled conditions, although the water
content was systematically lower in the vernalized plants
grown under controlled conditions.
General discussion
NMR as a phenotyping tool in field conditions
The results of the present study show that a mobile NMR
lab makes it possible to successfully perform outdoor
NMR measurements under controlled conditions usually
obtained in the laboratory that ensure optimum meas-
urement accuracy. Using this approach, it was possible to
compare the NMR signal from plants grown under well-
controlled conditions (growth cabinet) with the signal
from the plants grown in the field.
The trend in the transverse relaxation times associated
with vacuolar water revealing changes in leaf structure
Fig. 5 Changes in chlorophyll content (a), dry weight (b) and water
content (c) during leaf development according to the NMR split
scale. The results of the field experiment are compared with the
results from obtained under controlled conditions [7]—Sorin et al.,
Botanical Studies 2016, 57; acknowledgment to Springer; [8]—Sorin
et al., Planta. 2015, 24; copyright Springer. As the leaves were rear-
ranged according to the NMR split scale after sampling, in the case
of the field experiment the values corresponding to the leaf ranks
between 0 and −10 are the averages ± standard deviations of data
collected from leaves of four plants, for leaf rank −11 the data is the
average ± standard deviations of data collected from leaves of three
plants. For leaf ranks between −15 and −12 and between 3 and 1
less than tree measurements were available for analysis
8. Page 8 of 11
Musse et al. Plant Methods (2017) 13:53
during senescence was very similar to that previously
measured on plants grown under controlled conditions.
This shows that, despite the great variability of the envi-
ronmental factors during plant growth and through-
out the canopy (marked variations in temperature and
humidity, light exposure, wind, etc.), the NMR method
can provide robust indicators of the leaf development
stage of plants grown in the field. The results of the pre-
sent outdoor study, confirmed previous data [7–9] show-
ing that enlargement of palisade cells reflected by the
signal split correspond to a rather late leaf senescence
event in oilseed rape leaves. However, the present study
using data collected from a wider range of leaf ranks also
demonstrated that structural changes were initiated very
early in mature leaves. Indeed, the physiological process
at the origin of this late event (split of the NMR compo-
nent corresponding to the vacuolar water) highlighted by
a continuous increase in the T2 value of the longest com-
ponent corresponding to vacuolar water appears to have
been initiated while the chlorophyll and dry matter con-
tent were still high.
Within the common framework of changes in relaxa-
tion times, some differences in the NMR param-
eters measured on plants grown under different
conditions were observed and are discussed in the fol-
lowing paragraph.
Impact of the environmental conditions on leaf
development
As mentioned above, some differences were observed
in the NMR parameters measured on plants grown
under different conditions (Fig. 4a, b). These differ-
ences revealed modifications in leaf development pat-
tern caused by heterogeneous climate conditions with
marked and abrupt changes in temperature and/or
humidity. Although the T2 of the signal component asso-
ciated with vacuolar water before the split (leaf rank 0)
was very similar, a difference was observed in the leaf
ranks at which the maximum rate of the increase in the
T2 value occurred. This indicated that changes in the
vacuolar volume associated with the T2(5) increase [8]
were precocious in field plants. This is in accordance with
the measurements of chlorophyll content (Fig. 5a) and is
probably explained by more marked changes in environ-
mental conditions during leaf development.
The environment perceived by plants has an effect on
the progress of leaf senescence but not necessarily on the
onset of senescence [31]. It is well known that several fac-
tors, including an unbalanced sink-source ratio, may ini-
tiate leaf senescence. For instance, in the case of a high
source-sink ratio and photoassimilates, inhibition of feed-
back by photosynthesis may trigger senescence, whereas
in opposite high sink activity may trigger leaf senescence
through the remobilization of nutrients (mainly nitrogen)
[32]. The effect of N status on the induction of senescence
may also be driven by plant archichecture and the quantity
and quality of light that reaches the leaf [33]. In sunflower,
it has been reported that nitrogen export is promoted by a
low red/infra-red ratio rather than by the amount of light
[34]. Nevertheless, in the present study, it seems that the
main discriminating factor at the origin of the differences
observed in the senescence patterns described by NMR
signal components corresponding to the vacuolar water
and other parameters was the vernalization process and
the physiological status of plants and not to the hetero-
geneous conditions prevailing in the field experiment (in
contrast to the homogenous conditions in the growth
cabinet). The role of vernalization in the regulation of
leaf senescence has been reviewed in [35]. Cold tempera-
tures can affect both plant development and leaf senes-
cence [36]. Vernalization has an impact through different
mechanisms. While infertility increases the life span of
the whole plant through the production of additional
young leaves [37], as was the case in the non-vernalized
plants analyzed in [8], the vernalization process initiates
the development of the new reproductive organ with high
sink activity. It has also been reported that leaf senescence
may speed up with flowering [38]. In the present study,
measurements were made at the stem elongation stage
during which the process of nutrient remobilization from
the old leaves was emphasized. Finally, it should be noted
that, in the experiment corresponding to the non-vernal-
ized plants [8], the nutritive solution was supplied twice
a week, while in the case of the vernalized plants ([7] and
the present study) N was supplied according to the plant
development. In both cases, the plants were grown under
optimum nutrient conditions but in the case of the vernal-
ized plants, the level of the available nutrients in the soil
was irregular.
The young leaves from the vernalized plants (present
experiment and [7]) were characterized by higher dry
weight than those of the non-vernalized plants [8]. This
is probably due to a more active cell division induced by
cold stress [39] associated with a higher number of cell
layers. However, the dry mass decreased markedly in
senescing leaves sampled on vernalized plants, whereas
the decrease was minor in leaves taken from non-vernal-
ized plants. This phenomenon has been explained by the
appearance of large intercellular grass-filled spaces and a
resulting decrease in the number of cells [7]. This means
that although senescence is characterized by an increase
in vacuole volume [7–9] reflected by an increase in water
content and in T2, the total amount of the vacuolar water
expressed by LWW (4 + 5) decreased in senescing leaves.
Concerning the NMR signal components correspond-
ing to water in cell compartments other than vacuoles
9. Page 9 of 11
Musse et al. Plant Methods (2017) 13:53
(T2 range up to 30 ms), very similar results were obtained
in the field experiment and in the experiments conducted
under controlled conditions in about 50% of the leaves
analyzed. In the remaining leaves, the number of compo-
nents detected in this T2 range was one or three, instead
of two components as expected from [7, 8]. Consider-
ing that in all cases, the sum of the intensities of all these
components (one, two or three) represented appromi-
mately the same percentage of the total signal for a given
leaf rank, it can be assumed that these T2 peaks corre-
sponded to the same water pools (apoplastic water and
water inside starch granules and chlorophyll) as proposed
in [8]. However, due to the heterogeneous environmen-
tal factors (for example, the amount of light received by
the leaves), it was not possible to distinguish the corre-
sponding water pools according to their T2 values in all
cases. It will be interesting to clarify this point in further
experiments, although this is not crucial for the purpose
of phenotyping.
Considering a negative shift of five leaf ranks for the
chlorophyll content curve (Fig. 5a) corresponding to the
non-vernalized plants, a perfect match was observed
in the three sets of data. It would be therefore possible
to argue that structural changes associated with senes-
cence occur earlier in non-vernalized plants. This can be
explained by the fact that, as mentioned above, leaf tis-
sues of non-vernalized plants had fewer cell layers and a
thinner cuticle. For this reason, it is possible to conclude
that (1) structural changes occurring in the leaf through-
out the canopy are a constant feature in the development
of the leaf in oilseed rape independently of environmen-
tal and growing conditions, (2) it is possible to monitor
these structural changes with NMR relaxometry in the
field as in a controlled environment, but (3) the NMR
split scale should only be used for comparison within a
set of measurements made in the same set of growing
conditions.
NMR experiment in outdoor conditions
Until now, only a few mobile NMR [14] and MRI [27–29]
systems have been used to investigate plants in their nat-
ural (outdoor) environment. Although the constraints of
NMR and MRI approaches are not exactly the same, both
techniques have to deal with the effects of the environ-
mental conditions on the NMR/MRI devices. All outdoor
measurements were performed with permanent mag-
nets that are the best suited for mobile devices but have
the disadvantage of being very sensitive to variations in
temperature. In the first instance, the temperature drift
of the magnetic field is limited by thermal insulation of
the magnet [27, 28] and is generally further compen-
sated by using the field frequency lock approach. How-
ever, because changes in air temperature in outdoor
environments are usually much larger than in standard
laboratories, shifts in temperature can be a major prob-
lem in outdoor measurements [26]. Another problem
with outdoor measurements is the role of the tempera-
ture variations in the NMR signal. In plant tissues, the
dependence of the distribution of water proton trans-
verse relaxation times on temperature is complex, as
several contributions may overlap [40]. Indeed, the tem-
perature affects molecular mobility and the diffusive
exchange of water between the subcellular compartments
(diffusion coefficients and membrane permeability). As
a result, both T2 and peak amplitudes change with tem-
peraure, and are thus a possible source of interpretation
errors.
Malone et al. [24] isolated the variation in the signal
due to biological factors from that due to changes in the
temperature of the detector. These authors characterized
the sensitivity of their system to temperature and built a
model that accounted for the particular linear depend-
ence of the detector circuitry on temperature for the pre-
diction of the variation in the NMR signal. The method
was shown to be effective in a greenhouse experiment in
which the average daily temperature variation was 10 K,
with an average daily high near 305 K. However it should
be tested for higher temperature variations before the
method is used in outdoor conditions. The temperature
stability of the samples is also critical for measurements
of relaxation times as both signal ampliture and relaxa-
tion times depend to a great extent on temperature. This
issue has been addressed in only a limited number of
studies. Windt et al. [17] addressed a problem of varia-
tions in the temperatures of the sample and of the spec-
trometer by continuously monitoring the temperature of
the tree stem in the NMR device and the temperature of
the spectrometer. They observed slight differences in the
amplitude of the NMR signal, which they attributed to
variations in the temperature of the object (due to a shift
in the Boltzmann equilibrium) and temperature-induced
differences in the signal amplification factor of the spec-
trometer. A temperature correction factor was applied
to compensate for these differences. Another possible
approach is to equip the NMR device with a temperature
control device for a sample. Anferova et al. [41] devel-
oped two different types of mobile temperature control
units compatible with the NMR-MOUSE for analysis
of rubber by transverse NMR relaxation. For this appli-
cation, stable temperatures of the samples were criti-
cal, and temperature stability of better than 0.5 °C was
needed for good reproducibility of measurements. The
use of the device developed and tested here extends the
range of possible investigations with the NMR-MOUSE
to samples in environments with marked variations in
temperature.
10. Page 10 of 11
Musse et al. Plant Methods (2017) 13:53
In the present study, the effects of the environmen-
tal conditions on both the device and the sample were
controlled. The operating temperature of the benchtop
NMR device magnet was maintained through a mag-
net heating system. The stable temperature of the sam-
ple was ensured by a variable temperature control units
within 0.1 °C. The NMR mobile laboratory therefore
made it possible to perform measurements in exactly the
same controlled conditions throughout the experiment,
despite the fact the measurements were performed in an
environment with no temperature regulation.
As described in “Methods”
, after the NMR experiment,
the samples were transferred to the laboratory for the deter-
mination of water content and dry weight of leaves. These
measurements were not possible in the field, as weighing
the samples requires a precision balance, which was shown
to be sensitive to transport. However, by measuring the
free induction decay (FID) signal in addition to the CPMG
curve, it is possible to descriminate between water and dry
matter and to use the relationship established in [9] to esti-
mate the water content and dry mass of the leaf samples.
This would make it possible to overcome the difficulty of
using a balance in the field in further experiments.
Conclusion
The mobile NMR laboratory developed in this study was
shown to be able to perform accurate outdoor charac-
terization of oilseed rape leaves throughout the canopy.
The approach used enabled in situ assessment of physio-
logical status of leaves from plants grown in their natural
environment without disturbing the plants themselves.
The study enabled the comparison between the patterns
of NMR signal evolution from plants grown under well-
controlled conditions and plants grown in the field. The
method described here provides new opportunities for
fine phenotyping and monitoring of plant development
in the natural environment. It can be used for the selec-
tion of oilseed rape genotypes with high tolerance to
water or nitrogen depletion. Future investigations should
extend the method to other crops.
Authors’contributions
All the authors cooperated on all the experiments reported in this article
and participated in the data analyses. All authors read and approved the final
manuscript..
Author details
1
IRSTEA, OPAALE, 17, avenue de Cucillé, 35044 Rennes Cedex, France.
2
Université Bretagne Loire, Rennes, France. 3
INRA, UMR 1349 IGEPP-Institut
de Génétique, Environnement et Protection des Plantes, UMR INRA – Agro-
campus Ouest-Université de Rennes 1, Domaine de la Motte, 35653 Le Rheu
Cedex, France.
Acknowledgements
This work was supported by the program“Investments for the Future”(Project
ANR-11-BTBR-0004“RAPSODYN”). We are most grateful to the PRISM core
facility (Rennes, France) for access to the facilities, the Genetic Resources Center
(Bracy Sol, BRC, UMR IGEPP, INRA Ploudaniel, France) for providing the seeds of
the Aviso variety and the Experimental Unit of“La Motte”(INRA Le Rheu, France)
for the trial setup and management. We thank Françoise LE CAHEREC, Marie-
Françoise NIOGRET, Carole DELEU, Nathalie NESI, Anne LAPERCHE and Christine
BISSUEL (IGEPP) for setting up the field experiment and for cooperation on this
study. We also thank Michel Loubat and Yves Diascorn (IRSTEA) for the technical
assistance in setting up the mobile NMR lab and Patrick Leconte, Elise Alix and
Bernard Moulin (IGEPP) for technical support in plant management.
Competing interests
The authors declare that they have no competing interests
Availability of data and materials
All data generated or analyzed during this study are included in this published
article [and its supplementary information files].
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in pub-
lished maps and institutional affiliations.
Received: 23 May 2017 Accepted: 20 June 2017
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