Transgenic plants are plants whose genome has been altered by adding one or more transgenes. The first transgenic plant was produced in 1982 by adding an antibiotic resistance gene to tobacco. Since then, transgenic crops with traits like herbicide resistance, insect resistance, drought tolerance, nutrient enhancement, and pharmaceutical production have been developed using gene transfer methods like Agrobacterium-mediated transformation. Transgenic plants offer benefits like increased yield, stress resistance, and low-cost pharmaceuticals, but also raise biosafety concerns that must be addressed.
Tissue culture and virus indexing for the production of clean planting materialsILRI
Poster prepared by P. Asami, C. Too, M. Macharia, P. Niyonzima, D. Bigirimanaa, G. Ndarubayemwo, D. Beyene, J. Harvey and T.A. Holton for the ILRI APM 2013, Addis Ababa, 15-17 May 2013
To decrease our world hunger and to make the plant more nutritious the transgenic technique was developed. This the basis of the transgenic plant and its technique
Tissue culture and virus indexing for the production of clean planting materialsILRI
Poster prepared by P. Asami, C. Too, M. Macharia, P. Niyonzima, D. Bigirimanaa, G. Ndarubayemwo, D. Beyene, J. Harvey and T.A. Holton for the ILRI APM 2013, Addis Ababa, 15-17 May 2013
To decrease our world hunger and to make the plant more nutritious the transgenic technique was developed. This the basis of the transgenic plant and its technique
Vector mediated gene transfer methods for transgenesis in Plants.Akshay More
Presentation include Vector mediated gene transfer methods for trans-genesis in Plants. Only Vector-based methods are covered. Vectors includes Bacteria, Viruses, transposable genetic elements. Other possible vectors for transgenesis are also covered.
An overview of the Agrobacterium-mediated gene transfer process. Moreover, studied different kinds of Agrobacterium species are involved in this mechanism.
Agrobacterium is a rod-shaped, Gram-negative bacteria found mostly in the soil. It is a plant pathogen that is responsible for causing crown gall disease in them. This bacteria is also known as the natural genetic engineer because of it's the ability to integrate its plasmid Gene into the plant genome.
Agrobacterium tumefaciens transfer of their genetic material T-DNA of Ti-plasmid into the plant cell: A: Agrobacterium tumefaciens; B: Agrobacterium genome; C: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genes; D: Plant cell
A Ti-Plasmid (tumor-inducing plasmid) is a ds, circular DNA that often, but not always. It's a piece of genetic equipment that transfers genetic material from bacterial cells means Agrobacterium tumefaciens into plant cells used to induce tumors in the plant. The Ti-plasmid is damage when Agrobacterium is grown above 28 °C. Such cured bacteria don't induce crown gall disease in the plant due to they are avirulent. The Ti-Plasmid are classified into two types on the basis of opine genes are present in T-DNA.
The Plasmid has 196 genes that code for 195 proteins. There is no one structural RNA. The plasmid is 206.479 nucleotides long. the GC content is 56% and 81% of the genetic material is coding genes.
The modification of this plasmid is a very important source in the production of transgenic plants.
The T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS).
In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognized by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. So that the T-DNA can integrate into the host genome.
We inoculate Agrobacterium containing our genes of interest, onto wounded plant tissue explants. The Agrobacterium then transfers the gene of interest into the DNA of the plant tissue.
Biotechnology - Plant Biotechnology (Transgenic plants, Herbicide Resistant Plants, Glyphosate Tolerant Plants, Sulphonylurea Tolerant Plants, Atrazine Tolerant Plants, Phosphinothricin Tolerant Plants, Bromoxynil Tolerant Plants, Insect Resistant Plants, Animal Cells, Plant Cells, Tissue Cultures, Viruses, Prokaryotes)
Plant biotechnology is a precise process in which scientific techniques are used to develop molecular and cellular based technologies to improve plant productivity, quality and health; to improve the quality of plant products; or to prevent, reduce or eliminate constraints to plant productivity caused by diseases, pest organisms and environmental stresses. It can be defined as human intervention on plant material by means of technological instruments in order to produce permanent effects, and includes genetic engineering and gene manipulation to obtain transgenic plants.
See more
https://goo.gl/Tf0VTG
https://goo.gl/DySPuV
https://goo.gl/cncY7m
Contact us
Niir Project Consultancy Services
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Plant Biotechnology, Biotechnology Industry in India, Opportunities in Biotechnology and Business, Commercialization of Plant Tissue Culture in India, Agricultural Biotechnology, Biotechnology Industry in India, Biotechnology in India, Plant Biotech, Plant Tissue Culture, Plant Biotechnology and Agriculture, Plant Biology & Plant Biotechnology, Profitable Biotechnology Business Ideas, Small Business Ideas in Plant Biotechnology Industry, How to Start Small Scale Plant Biotech Industry in India, Start Tissue Culture Biotechnology, Plant Biotechnology Ideas for Small Business, Plant Biotechnology Business Plan, Plant Biotechnology Ideas, Plant Biotechnology Startups in India, Organisms of Biotechnology, Animal Cells, Plant Cells, Transgenic Plants, Herbicide Resistant Plants, Glyphosate Tolerant Plants, Sulphonylurea Tolerant Plants, Atrazine Tolerant Plants, Phosphinothricin Tolerant Plants, Bromoxynil Tolerant Plants, Insect Resistant Plants, Transgenic Plants With Cowpea Trypsin Inhibitor, Transgenic Plants With Viral Coat Protein, Transgenic Plants With Viral Nucleoprotein, Transgenic Plants With Viral SAT RNA, Transgenic Plants With Antisense RNA, Transgenic Plants Resistant to Fungi and Bacteria, Transgenic Plants With Improved Storage Proteins, Stress Tolerant Plants, Cold Tolerant Plants, Drought Tolerant Plants, Pharmaceutical Compounds, Biodegradable Plastics, Biological Nitrogen Fixations, Non-Symbiotic Nitrogen Fixation, Non-Symbiotic Nitrogen Fixation, Nif-Genes of Azotobacter, Nif-Genes of Anabaena, Legume Nodulin Genes, Transfer of Nif Genes to Yeasts, Transfer of Nif-Genes to Plants, Transfer of Hup Genes
Agrobacterium mediated Transformation-Mechanism of gene transfer,Virulence induction in presence of Plant secondary metabolites,Chromosomal genes and Vir genes,Agrobacterium tumefaciens – pathogen and useful tool for genetic engineering
Plant biotechnology also known as green biotechnology is the use of biotechnology in plant or crop production. There are several techniques used such as ell culturing. Organ culture, explant culture, cell suspension culture are some culture types. This is a very useful technology in which have several applications like synthetic seed production, somaclonal variation, cybridization, hybridization.
It deals with application of such genes and proteins obtained from the animals especially for medicine and also industries. It is much useful to understand the basic.
Somaclonal Variation in Plant tissue culture - Variation in somaclones (somatic cells of plants)
Somaclonal variation # Basis of somaclonal variation # General feature of Somaclonal variations # Types and causes of somaclonal variation # Isolation procedure of somaclones via without in-vitro method and with in-vitro method with their limitations and advantages # Detection of isolated somaclonal variation # Application (with examples respectively related to crop improvement) # Advantages and disadvantages of somaclonal variations.
https://www.youtube.com/watch?v=IZwrkgADM3I
Also watch, Gametoclonal variation slides to understand, how to changes occur in gametoclones of plants.
https://www.slideshare.net/SharmasClasses/gametoclonal-variation
Vector mediated gene transfer methods for transgenesis in Plants.Akshay More
Presentation include Vector mediated gene transfer methods for trans-genesis in Plants. Only Vector-based methods are covered. Vectors includes Bacteria, Viruses, transposable genetic elements. Other possible vectors for transgenesis are also covered.
An overview of the Agrobacterium-mediated gene transfer process. Moreover, studied different kinds of Agrobacterium species are involved in this mechanism.
Agrobacterium is a rod-shaped, Gram-negative bacteria found mostly in the soil. It is a plant pathogen that is responsible for causing crown gall disease in them. This bacteria is also known as the natural genetic engineer because of it's the ability to integrate its plasmid Gene into the plant genome.
Agrobacterium tumefaciens transfer of their genetic material T-DNA of Ti-plasmid into the plant cell: A: Agrobacterium tumefaciens; B: Agrobacterium genome; C: Ti Plasmid : a: T-DNA , b: Vir genes , c: Replication origin , d: Opines catabolism genes; D: Plant cell
A Ti-Plasmid (tumor-inducing plasmid) is a ds, circular DNA that often, but not always. It's a piece of genetic equipment that transfers genetic material from bacterial cells means Agrobacterium tumefaciens into plant cells used to induce tumors in the plant. The Ti-plasmid is damage when Agrobacterium is grown above 28 °C. Such cured bacteria don't induce crown gall disease in the plant due to they are avirulent. The Ti-Plasmid are classified into two types on the basis of opine genes are present in T-DNA.
The Plasmid has 196 genes that code for 195 proteins. There is no one structural RNA. The plasmid is 206.479 nucleotides long. the GC content is 56% and 81% of the genetic material is coding genes.
The modification of this plasmid is a very important source in the production of transgenic plants.
The T-DNA must be cut out of the circular plasmid. A VirD1/D2 complex nicks the DNA at the left and right border sequences. The VirD2 protein is covalently attached to the 5' end. VirD2 contains a motif that leads to the nucleoprotein complex being targeted to the type IV secretion system (T4SS).
In the cytoplasm of the recipient cell, the T-DNA complex becomes coated with VirE2 proteins, which are exported through the T4SS independently from the T-DNA complex. Nuclear localization signals, or NLS, located on the VirE2 and VirD2 are recognized by the importin alpha protein, which then associates with importin beta and the nuclear pore complex to transfer the T-DNA into the nucleus. So that the T-DNA can integrate into the host genome.
We inoculate Agrobacterium containing our genes of interest, onto wounded plant tissue explants. The Agrobacterium then transfers the gene of interest into the DNA of the plant tissue.
Biotechnology - Plant Biotechnology (Transgenic plants, Herbicide Resistant Plants, Glyphosate Tolerant Plants, Sulphonylurea Tolerant Plants, Atrazine Tolerant Plants, Phosphinothricin Tolerant Plants, Bromoxynil Tolerant Plants, Insect Resistant Plants, Animal Cells, Plant Cells, Tissue Cultures, Viruses, Prokaryotes)
Plant biotechnology is a precise process in which scientific techniques are used to develop molecular and cellular based technologies to improve plant productivity, quality and health; to improve the quality of plant products; or to prevent, reduce or eliminate constraints to plant productivity caused by diseases, pest organisms and environmental stresses. It can be defined as human intervention on plant material by means of technological instruments in order to produce permanent effects, and includes genetic engineering and gene manipulation to obtain transgenic plants.
See more
https://goo.gl/Tf0VTG
https://goo.gl/DySPuV
https://goo.gl/cncY7m
Contact us
Niir Project Consultancy Services
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Plant Biotechnology, Biotechnology Industry in India, Opportunities in Biotechnology and Business, Commercialization of Plant Tissue Culture in India, Agricultural Biotechnology, Biotechnology Industry in India, Biotechnology in India, Plant Biotech, Plant Tissue Culture, Plant Biotechnology and Agriculture, Plant Biology & Plant Biotechnology, Profitable Biotechnology Business Ideas, Small Business Ideas in Plant Biotechnology Industry, How to Start Small Scale Plant Biotech Industry in India, Start Tissue Culture Biotechnology, Plant Biotechnology Ideas for Small Business, Plant Biotechnology Business Plan, Plant Biotechnology Ideas, Plant Biotechnology Startups in India, Organisms of Biotechnology, Animal Cells, Plant Cells, Transgenic Plants, Herbicide Resistant Plants, Glyphosate Tolerant Plants, Sulphonylurea Tolerant Plants, Atrazine Tolerant Plants, Phosphinothricin Tolerant Plants, Bromoxynil Tolerant Plants, Insect Resistant Plants, Transgenic Plants With Cowpea Trypsin Inhibitor, Transgenic Plants With Viral Coat Protein, Transgenic Plants With Viral Nucleoprotein, Transgenic Plants With Viral SAT RNA, Transgenic Plants With Antisense RNA, Transgenic Plants Resistant to Fungi and Bacteria, Transgenic Plants With Improved Storage Proteins, Stress Tolerant Plants, Cold Tolerant Plants, Drought Tolerant Plants, Pharmaceutical Compounds, Biodegradable Plastics, Biological Nitrogen Fixations, Non-Symbiotic Nitrogen Fixation, Non-Symbiotic Nitrogen Fixation, Nif-Genes of Azotobacter, Nif-Genes of Anabaena, Legume Nodulin Genes, Transfer of Nif Genes to Yeasts, Transfer of Nif-Genes to Plants, Transfer of Hup Genes
Agrobacterium mediated Transformation-Mechanism of gene transfer,Virulence induction in presence of Plant secondary metabolites,Chromosomal genes and Vir genes,Agrobacterium tumefaciens – pathogen and useful tool for genetic engineering
Plant biotechnology also known as green biotechnology is the use of biotechnology in plant or crop production. There are several techniques used such as ell culturing. Organ culture, explant culture, cell suspension culture are some culture types. This is a very useful technology in which have several applications like synthetic seed production, somaclonal variation, cybridization, hybridization.
It deals with application of such genes and proteins obtained from the animals especially for medicine and also industries. It is much useful to understand the basic.
Somaclonal Variation in Plant tissue culture - Variation in somaclones (somatic cells of plants)
Somaclonal variation # Basis of somaclonal variation # General feature of Somaclonal variations # Types and causes of somaclonal variation # Isolation procedure of somaclones via without in-vitro method and with in-vitro method with their limitations and advantages # Detection of isolated somaclonal variation # Application (with examples respectively related to crop improvement) # Advantages and disadvantages of somaclonal variations.
https://www.youtube.com/watch?v=IZwrkgADM3I
Also watch, Gametoclonal variation slides to understand, how to changes occur in gametoclones of plants.
https://www.slideshare.net/SharmasClasses/gametoclonal-variation
Bio saftey in transgenics & its productsVipin Shukla
Transgenic plants are those plants were we insert an foreign gene in an host genome to modify its characters such as Stress tolerance, Virus resistant, Biotic and Abiotic Tolerance etc.
plant Biotechnology: The application of Plant Biotechnology by use of scientific method to manipulate living cells or organisms for practical uses (manipulation and transfer of genetic material).
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
2. terms to know
TRANSGENE- It is a foreign gene or genetic
material that has been transferred naturally or by
any of a number of genetic engineering techniques
from one organism to another.
TRANSGENESIS- The phenomenon of introduction
of exogenous DNA into the genome to create and
maintain a stable and heritable character.
TRANSGENIC PLANTS- The plant whose genome
is altered by adding one or more transgenes are
known as transgenic plants.
3. history
1982
• 1st transgenic plant produced which is an antibiotic resistance tobacco
plant.
1984
• 1st successful plant genetic engineering experiments using caulimovirus
vector.
1994
• 1st genetically modified crop approved for sale in U.S. was FlavrSavr
tomato.
1995
• 1st pesticide producing crop, Bt Potato was approved by U.S.
Environmental ProtectionAgency
1996
• 1st genetically modified flower Moondust, bluish colored carnation, was
introduced.
2000
• Golden rice with β- carotene developed with increased nutrient value.
2013
• 1st genetically engineered crop developed by Robert Fraley, Marc Van
Montagu &Marry Dell Chilton were awarded World Food Prize.
4. Why transgenic plants?
TRANSGENIC PLANTS
NUTRITIONAL
QUALITY
HERBICIDE
RESISTANC
E
INSECTICID
E
RESISTANC
E
BIOTIC
STRESS
TOLERANC
E
ABIOTIC
STRESS
TOLERANC
E
ENHANCED
SELF LIFE
INDUSTRIAL
PRODUCT
PHARMACE-
UTICALS
&VACCINES
6. AGROBACTERIUM MEDIATED GENETRANSFER
Agrobacterium is treated as
nature’s most effective
plant genetic engineer.
A.tumifaciens infects
wounded or damaged plant
tissues results in the
formation of plant tumor
called crown gall.
The bacterium releases Ti
plasmid into the plant cell
cytoplasm which induce
crown gall.
Several dicots are affected
by this disease e.g. grapes,
roses, etc.
7. ORGANIZATION OF TI PLASMID
The size of Ti plasmid is approx. 200 kb.
The Ti plasmid has three important regions:
(i) T-DNAregion
(ii) Virulence region
(iii) Opine catabolism region
There is ori region that is responsible for the origin of DNAreplication.
8. T-DNA transfer and integration
Fig: Diagrammatic representation of T-DNA transfer &its integration into host
plant cell genome
10. PLANT VIRusVECTORS
Plant viruses are considered as efficient gene transfer agents as they can
infect the intact plants and amplify the transferred genes through viral
genome replication.
It has some limitations like that vast majority of plant viruses have
genome not of DNA but of RNA. Two classes of DNA viruses are known
to infect higher plants, caulimovirus and geminivirus & neither is ideally
suited for gene cloning.
i) CAULIMOVIRUS:
They contain circular dsDNA and are spherical in shape. The
caulimovirus group has around 15 viruses & among these Cauliflower
Mosaic Virus(CaMV) is the most important for gene transfer.
CaMV infects many plants & can be easily transmitted. The infection
is systemic. It’s genome does not contain any non-coding regions.
11. It has certain limitations-
i)CaMV vectors has a limited capacity for insertion of foreign
genes.
ii)Infective capacity of CaMV is lost if more than a few hundred
nucleotides are introduced.
iii) Helper viruses cannot be used since the foreign DNA gets
expelled and wild-type viruses are produced.
II) GEMINIVIRUS:
They contain one or two circular ssDNA. They are particularly
interesting because their natural host include plant size such as
maize & wheat. They could therefore be potential vector for these
and other monocots.
They have their own set of difficulties-
It is very difficult to introduce purified viral DNA into theplants.
12. PHYSICAL GENE TRANSFER METHODS
1) ELECTROPORATION
Electroporation involves the creation of pores in the cell membrane
using electric pulse of high field strength. If DNA is present in the buffer
solution at sufficient concentration, it will be taken up through these
pores.
13. 2) PARTICLE BOMBARDMENT
It is also known as microprojectile bombardment, biolistics, gene gun, etc.
Foreign DNA coated with high velocity gold or tungsten particles to
deliver DNA into cells.
This method is widely being used because of its ability to transfer foreign
DNA into the mammalian cells and microorganisms.
14. 3) MICROINJECTION
Microinjection is a direct physical method involving the mechanical
insertion of the desirable DNA into a targetcell.
The technique of microinjection involves the transfer of the gene
through a micropipette into the cytoplasm or nucleus of a plantcell
or protoplast.
The most significant use of this is the introduction of DNA intothe
oocyte and the eggs of animals, either the transient expression
analysis or to generate transgenic animals.
The major limitations of microinjection are that it is slow, expensive,
and has to be performed by trained and skilled personnel.
15. 4) LIPOSOME MEDIATED TRANSFORMATION
Liposome mediated transformation involves adhesion of liposomes to the
protoplast surface, its fusion at the site of attachment and release of
plasmids inside the cell.
16. CHEMICAL GENE MEDIATED TRANSFER
1) POLYETHYLENE GLYCOL- MEDIATED TRANSFORMATION
Polyethylene glycol (PEG), in the presence of divalent cations,
destabilizes the plasma membrane of protoplasts and renders it permeable
to naked DNA.
A large number of protoplasts can be simultaneously transformed.
This technique can be successfully used for a wide variety of plant
species.
It has certain limitations:
i) The DNA is susceptible for degradation and rearrangement.
ii)Random integration of foreign DNA into genome may result in
undesirable traits.
iii) Regeneration of plants from transformed protoplasts is a difficult task.
2) DEAE-DEXTRAN MEDIATED TRANSFER
The desirable DNA can be complexed with a high molecular weight
polymer diethyl amino ethyl(DEAE)dextran and transferred.
The major limitation of this approach is that it does not yield stable
transformants.
17. Marker genes for plant transformation
Some methods for selecting the transformed plant materials have been devised
by using a set of genes referred to as marker genes.
These marker genes are introduced into the plant material along with the target
gene. The marker genes are of two types:
i) Selectable marker genes- The selection is based on the survival of
transformed cells when grown on a medium containing a toxic substance
(antibiotic, herbicide, antimetabolite). This is due to the fact that the selectable
marker gene confers resistance to toxicity in the transformed cells, while the
non-transformed cells will get killed.
Some of them are given below:
Antibiotic resistance genes( Hygromycin phosphotransferase, hpt gene)
Herbicide resistance genes( Enolpyruvylshikimate phosphate synthase, epsps)
Antimetabolite marker genes( Dihydrofolate reductase, dhfr gene)
ii)Reporter genes- An assay for the reporter gene is carried out by estimating
the quantity of the protein it produces or the final products formed.
Some of the important ones are given below:
Opine synthase (ocs), β-Glucuronidase (gus), Bacterial luciferase (luxA),
Firefly luciferase (luc)
18. APPLICATIONS
Transgenic plants have various applications -:
RESISTANCE TO BIOTIC STRESS
1) INSECT RESISTANCE
2) VIRUS RESISTANCE
3) FUNGALAND BACTERIALRESISTANCE
RESISTANCE TO ABIOTICSTRESS
1) HERBICIDE RESISTANCE
2) GLYPHOSATE RESISTANCE
IMPROVEMENT OF CROP YIELD &QUALITY
3) EXTENDED SELF LIFE OF FRUITS
4) IMPROVED NUTRITION
5) IMPROVED COLORATION
PRODUCTION OF LOW-COSTPHARMACEUTICALS
1) EDIBLE VACCINES
2) ESSENTIALPROTEINS
19. Insect resistantplants
It is estimated that about 15% of the world’s crop yield is lost due to insects or
pests.
Bacillus thuringiensis was first discovered by Ishiwaki in 1901. It is a gram
negative soil bacterium.
Most of the Bt toxins are active against Lepidopteron larvae, while some of
them are specific against Dipterans and Coleopteran insects.
Different cry protein produced by Bacillus:
Cry I : kills butterflies and moths
Cry II : kills butterflies and flies
Cry III : kills beetles
Cry IV : kills only flies
Plant made only low levels of toxin because they are designed to express well in
bacteria and not in plants as they are produced from bacterium.
Insect toxin gene was altered by changing many bases of the third position of
the redundant codon to improve its toxicity.
21. Virus resistant plants
Plants may be engineered with genes for resistance to
viruses, bacteria, and fungi.
Virus-resistant plants have a viral protein coat gene
that is overproduced, preventing the virus from
reproducing in the host cell, because the plant shuts off
the virus protein coat gene in response to the
overproduction.
Coat protein genes are involved in resistance to
diseases such as cucumber mosaic virus, tobacco rattle
virus, and potato virus X.
22. Herbicide resistance
Weeds are unwanted and useless plants that grow along with thecrop
plants. To tackle these, herbicides are used.
Phosphoenolpyruvate
Compete
Shikimate
EPSPS
Glyphosate
Enolpyruvylshikimate-3- phosphate
Tryptophan Tyrosine
Phenylalanine
Fig: Glyphosate competes with the phosphoenolpyruvate in the EPSPS
catalyzed synthesis of enolpyruvylshikimate-3- phosphate and inhibits
synthesis of tryptophan, tyrosine and phenylalanine.
EPSPS- Enolpyruvylshikimate-3- phosphate synthase
The 1st crops to be engineered for glyphosate resistance were
produced by Monsanto Co. and called “Roundup Ready”.
23. Vaccine production
Potatoes have been studied using a portion of the E. coli enterotoxin
in mice and humans and then transgenic potatoes were produced.
Ingestion of this transgenic potato resulted in satisfactory
vaccinations and no adverse effects.
Other candidates for edible vaccines include banana and tomato,
and alfalfa, corn, and wheat are possible candidates for use in
livestock.
Edible vaccines are vaccines produced in plants that can be
administered directly through the ingestion of plant materials
containing the vaccine. Eating the plant would then confer
immunity against diseases.
One focus of current vaccine effort is on hepatitis B.
Transgenic tobacco and potatoes were engineered to
express hepatitis B virus vaccine.
24. Golden rice
Normal rice
GOLDEN RICE
Transgenic technology produced a type of rice that
accumulates β-carotene in rice grains.
When it is consumed, β-carotene is converted into vitamin-A.
It contains 37 mg/g of carotenoid of which 84% is β-carotene.
25. FLAVR-SAVR TOMATO
This is produced by antisense technology.
The polygalactouronase gene, which is responsible for fruit decayis
silenced.
26. Biopolymers andplants
a) Plant seeds may be a potential source for plastics that could
be produced and easily extracted.
b) A type of PHA (polyhydroxylalkanoate) polymer called
“poly-beta-hydroxybutyrate”, or PHB, is produced in
Arabidopsis or mustard plant.
c) PHB can be made in canola seeds by the transfer of three
genes from the bacterium Alcaligenes eutrophus, which
codes for enzymes in the PHB synthesis pathway.
d) A polymer called PHBV produced through Alcaligenes
fermentation, which is sold under the name Biopol.
27. Fig: Produced by Gene Silencing
TEARLESS ONION
COLOURFUL
CAULIFLOWER