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transgenic breeding

This document discusses transgenic plants and provides several examples. It begins by defining a transgenic as a genotype developed through genetic engineering containing a foreign or modified gene. Several successful commercial transgenic crops are mentioned, including Bt cotton, golden rice, and Flavr Savr tomato. The document then covers the process of creating transgenic plants including identifying genes of interest, cloning genes, transforming plant cells, and backcross breeding. Methods of gene transfer like Agrobacterium-mediated transformation and particle bombardment are described. The document concludes by outlining the development and objectives of first and second generation transgenic crops.

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Submitted By Chanda kumari Submitted to Dr. Anant Department of Genetics and Plant Breeding Lovely Professional University, Punjab AN ASSIGNMENT on Transgenics
What is a transgenic? Transgene Exotic gene added to a species through recombinant DNA technology The organism that develop after successful transformation - transgenic BT COTTON GOLDEN RICE FLAVR SAVR TOMATO  Introduction
What is Transgenic Breeding ? Genetic improvement of crop plants, domestic animals, microbes through Biotechnology. What is Transgenics ? A genotype developed by the process ofgenetic engineering. Or A genotype containing foreign gene or modified geneof different species transferred by process of genetic engineering. What is Transgene ? Foreign gene or modified gene of any species which is used for development of transgenics. These may be from related wild species, microbes (Bacteria, viruses and fungi) or unrelated species.
Fig 1: A diagram re-drawn from Harlan and de Wet (1971) to include a description of gene pools as they relate to the use of transgenes . Circles are representations of the primary- (GP-1), secondary- (GP-2), tertiary- (GP-3), and quaternary-gene pools (GP-4)
Global area (Million Hectares) of Biotech crops, 1996-2016 by Country and Mega-Countries Source: ISAAA, 2016
Global Adoption Rates (%) for Principal Biotech Crops 2017 (Million Hectares) Trait Distribution in Approved Events, 1992-2016
Status of Biosafety Research Trails of Biotech Crops in India, 2016 Source: MOEF&CC, 2016, Analyzed by ISAAA,2016
Why Create Transgenic Plants? 1. Improve agricultural value of plant increase yield (herbicide-resistance, pest- resistance) enhance nutrition enhance taste 2. Plants can produce proteins for human needs (pharmaceutics) 3. Modified plants can be used to study effects of genes An entire plant can be regenerated from a single cell ***TOTIPOTENCY*** No separation of germ and somatic cells
Overview of The Process  There are five major steps involved in genetically engineering plants. These are DNA isolation, single gene cloning, gene designing, cell transformation, and backcross breeding. • DNA is extracted from an organism that has the desired trait. • The desired gene is located and copied. • The gene is inserted into a single plant cell using a transformation method. If the transgene successfully lands in the cells nucleus and is incorporated into one of the chromosomes, then the trait that it codes for will be expressed in the cell's offspring. • The cell multiplies and grows a new plant that contains the transgene in all of its cells. • Through backcross breeding the transgenic plant is crossed with a plant from a high yielding line. The resulting hybrids are the genetically modified plants that can enter the marketplace.
General procedure of recombinant DNA technology
APPLICATION OF TRANSGENIC PLANTS • Resistance to biotic and abiotic stresses
Improving crop yield and nutritional value
Help in alleviating poverty and hunger
Contribute to food, feed and fiber security
Transgenic plants as bioreactors for recombinant proteins
Provide better environment
Conserve biodiversity
GENE CONSTRUCT A typical plant gene Promoters /enhancers Reporter genes
METHODS FOR PLANT GENE TRANSFER INDIRECT METHODS (VECTOR-BASED) DIRECT METHODS (VECTOR-LESS ) • Agrobacterium mediated transfromation • Bacterial transformation • Conjugation • Phage transduction • Retroviral transduction • Transposition IN PLANTA TRANSFORMATION Physical methods • Particle bombardment • Electroporation • Microinjection • Liposome mediated DNA transfer • Silicon Carbide fibre mediated DNA transfer Chemical method • PEG-mediated DNA transfer • Floral Dip • Vacuum infiltration • Agro injection
Figure: A biolistic microprojectile gun. Source: http://en.wikipedia.org/wiki/Gene_gun (cc) DNA- or RNA-coated gold/tungsten particles are loaded into the gun and you pull the trigger.
PARTICLE BOMBARDMENT (BIOLISTICS)  The micro projectile bombardment method was initially named as biolistics by its inventor Sanford (1988).  Biolistics is a combination of biological and ballistics. There are other names for this technique- particle gun, gene gun, bio blaster.  Foreign DNA containing the genes to be transferred is coated onto the surface of minute gold or tungsten particles (1-3 micrometers) and bombarded onto the target tissue or cells using a particle gun.  Two types of plant tissue are commonly used for particle bombardment- Primary explants and the proliferating embryonic tissues.  Successfully used for the transformation of many cereals, e.g. rice, wheat, maize.  A commercially produced particle bombardment apparatus namely PDS-1000/HE is widely used these days.
Advantages : i. Gene transfer can be efficiently done in organized tissues. ii. Different species of plants can be used to develop transgenic plants. Limitations : iii. i. The major complication is the production of high transgene copy number. This may result in instability of transgene expression due to gene silencing. ii. The target tissue may often get damaged due to lack of control of bombardment velocity. Sometimes, undesirable chimeric plants may be regenerated.
VECTORS FOR THE PRODUCTION OF TRANSGENIC PLANTS Plasmid vectors • Ti plasmid • co integrative vector • Binary vectors Plant virus vectors
Bacillus Shigetane Ishiwatari (1901), first isolated thuringiensis. Bt is commonly abbreviated as Bacillus thuringiensis,is a gram-positive, facultative aerobic, rod-like, motile and sporulating bacterium. Bt is a naturally-occurring soil borne bacterium that is found worldwide Ubiquitous in nature. Produces crystals of endotoxin (Cry protien or delta toxin) - toxic to insect mainly in their larval stage, thus they act as insecticides. Bacillus thuringiensis
 These crystal proteins (Cry proteins) are insect stomach poisons.  Insects stop feeding within two hours of a first bite and, if enough toxin is eaten, die within two or three days  Important biological insect control agent.  Bt crystals, sometimes referred as insecticidal crystal proteins (ICP), are protein crystals formed during sporulation in some Bt strains coded by cry genes. Conti.....
• 1. Ingestion • 2. Solublization & proteolytic activation • 3. Binding to target site • 4. Formation of toxic lesions HOW Bt WORKS ?
Mode of Action of Bt Toxin in Insect Gut
Development of GM Crop/ Transgenics  Identify gene(s) giving a desired trait  Make copies of the gene  Transfer to plant tissue  Regenerate plants  Lab analysis and safety testing  Development of a variety  Field tests  Approval by Government agencies  Commercialization  Monitoring of efficacy and safety
Schematic Diagram of Bt Crop Improvement
Delivering the Gene to the Plant •Transformation cassettes are developed in the lab • They are then introduced into a plant •Two major delivery methods • Agrobacterium Tissue culture required to generate transgenic plants • Gene Gun
The Lab Steps
Cell Culture and Somatic Embryogenesis, a Means to Obtain TransgenicPlants Scheiden and Schwann made a critical observation, that plants possess a remarkable ability to generate free-living cells from plant tissues. Plant cell and tissue culture using sterile technique and in vitro (within glass) conditions are key elements to obtaining transgenic crop plants. Somatic Embryogenesis Totipotentcy: plant cell or cells are able to live independently, and also possess the ability to regenerate into a whole plant under the right environmental conditions. The first methodology of generating plants from cell culture is through the process of somatic embryo formation, whereby somatic cells (those not involved in sexual reproduction), produce an embryo similar to one produced by zygotic embryogenesis it is called somatic embryogenesis. Somatic embryos formed with root and shoot apical meristems are termed a bipolar embryo, and germinate into whole plants (Parrott, 2000). Organogenesis The second methodology of regenerating single cells into whole plants is termed organogenesis, where a meristematic cell from a root or shoot primordiums used to form organs (e.g. shoots, leaves or roots); these recovered organs can then be cultured into whole plants. Cell culture and transformation
Transgenic Crops: Development Objectives First-generation GM crops Herbicide resistance:- Corn,Soybean,rice,and Sugar beet Insect Pest resistance:- Corn, rice tomato and potato Viral resistance:- Papaya, Squash and potato Slow ripening and softening- Tomato and melon Improved oil quality -Canola and soybean Male sterility - Canola and corn
Second generation of GM crops Increased levels of protein Modified and healthier fats Modified carbohydrates Improved flavor characteristics  Increased levels of micronutrients Third generation of GM crops Resistance to abiotic stress “Pharmaplants” Conti..
Transgenic Breeding for Insect Resistance Crop Gene inserte d Resistance to Vector Achievement Year 1) Cotton Cry gene comple x Bollworm complex Agrobacteriu m tumefacience 1) 1 st Bt cotton in world by Monsanto Ltd. USA. 2) 1st Bt cotton in india - 1996 by MAHYCO - Monsanto Ltd. 1987 1996 2) Brinjal Cry 1 Ab Fruit and shoot borer Agrobacteriu m tumefacience 1) 1 st transgenic (Bt) Brinjal in world ( USA) 2004 3) Cabbage Cry gene comple x Cabbage worm and cabbage looper Agrobacteriu m tumefacience 1) 1 st transgenic (Bt) Cabbage in world (USA) 2002 4)Maize Cry gene complex European Corn Borer Agrobacterium tumefacience 1) 1st Transgenic maize in world is Maximizer (Spain) by Company Ciba Geigy 1995 5)Okra Cry 1 Ab Fruit and shoot borer Agrobacteriu m 1) 1 st Transgenic okra in world by U.S.A. 2002
Crop Gene inserted Resistance to Vector Achievement Year 6) Potato Cry-gene complex Colorado potato beetle Agrobacteriu m tumefacience First transgenic potato in world – Monsanto Ltd. U.S.A. 1995 7) Apple Cry-1-Ac Codling moth Agrobacteriu m tumefacience First transgenic Apple in world by U.S.A. 2000 8) Soybean Cry-1-Ac Leaf eating catterpilla r Agrobacteriu m tumefacience First transgenic Soybean in world – Monsanto Ltd. U.S.A. 1996 9) Sugar cane Cry-1-Ab Stem borer Agrobacterium tumefacience First transgenic Sugar cane in world – U.S.A. DNA plant technology company 1997 10) Tomato Cry-1-Ac Tobacco horn worm Agrobacteriu m tumefacience First transgenic Tomato in world – U.S.A by Calgene company 1987 11)Tobacco Trypsin inhibitor gene from cowpea Leaf eating catterpilla r Agrobacteriu m tumefacience First transgenic tobacco resistant to leaf eating catterpillar was developed in USA. 2001. Source – Esseentials of Plant Breeding
Transgenic breeding for keeping quality Crop Gene inserted Traits Vector Achievement 1) Tomato - a) Delay ripening a) Agrobacteriu m tumefacience b) Antisense RNA technology c)Agrobacteriu m tumefacience a) Variety : Endless summer by DNA plant technolog y company 1995 b) Delay fruit softening b) Flavr Savr b y Calgene company in 1994 c) Thicker skin and Alter c) By Zeneca compan
Crop Gene inserted Traits Vector Achievement 2) Apple ACC oxidase gene Delay ripening Agrobacteriu m tumefacience In scion cultivar ROYAL GALA is developed 3) Banana ACC gene Delay ripening Agrobacteriu m tumefacience Cavendish 1 4) Mango Rol C gene Storage and delay ripening Agrobacteriu m tumefacience Golden yellow ( Source - AGROBIAS Newsletter. 2006).
Transgenic breeding for disease resistance Crop Gene inserted Resistance to Vector Achievement 1) Potato PLRV-R PVX, PVY, PLRV Agrobacterium tumefacience First transgenic potato in world by – Monsanto Ltd. U.S.A. 2000 2) Cumcumber GUS and Markar gene NPT II CMV, ZYMV, WMV 2 Agrobacteriu m tumefacience First resistance variety Beit alpha MR 3) Faba bean Chitinase gene from Seratica marcescence Chocolate spot disease Plasmid First resistance variety in 2002 4) Banana FR gene Panama wilt Plasmid First resistance variety isCavendish 5) Papaya PRVR gene Papaya ring spot virus Plasmid First resistance variety is Sunup and second variety is Rainbow in 1999
Transgenic breeding for Herbicide Resistance Crop Gene transfer resistance to Vector Achivement 1.Rice Bar chimeric gene Hygromycine Plasmid Oryza sativa cv. IR 72 2.Nilgiri (Eucalyptus) Cry 3A Glufosinate ammonium(Liberty@ 6 l/ha) Agrobacteriu m tumefacience Variety-Ecofriend 3.Sugarcane PPTR( phospinothricin e resistant gene) Phosphinothricine Agrobacteriu m tumefacience I st herbicide resistant sugarcane was developed in USA. 4.Cotton Phosphinothric acetyl transeferase All non selective herbicides Particle gun bombardmen t method I st variety was releasedIn year 2000.
Transgenic breeding for adding Male sterility Crop Gene inserted Source of gene 1.Rapeseed mustard msi (male sterility iducing gene) Bacillus amyloliquefacien ce Source – Essentials of Plant Breeding , Transgenic breeding for drought resistance 1.Drought resistance in sorghum Gene transformed mlt D gene Vector Agrobacterium tumefacience Developed at CRIDA, Hyderabad Number of transgenics released 14.
2. Drought resistance in Wheat 1. Crop American bread wheat 2.Genes transformed mlt D and HVA 1. 3.Source of gene Osmotin gene complex from Atriplex numularia 4. By Microprojectile bombardment system Transgenic breeding for changing morphological character 1. Crop Petunia 2. Character modified Orange flower colour 3.Gene transferred Dihydroflavanal 4 reductase (FR) gene. 4.Source of gene Zea mays L. (Corn) Original Petunia Modified Petunia
Insect resistant cotton – Bt toxin kills the cotton boll worm  transgene = Bt protein Insect resistant corn – Bt toxin kills the European corn borer  transgene = Bt protein Agriculture Transgenics On the Market
Herbicide resistant crops  Now: soybean, corn, canola transgene = modified EPSP synthase or phosphinothricin-N-acetyltransferase Virus resistant papaya • transgene = virus coat protein
Next Generation of Ag Biotech Products Golden Rice – increased Vitamin A content transgene = three pathway enzymes Sunflower – white mold resistance transgene = oxalate oxidase from wheat
Global Status of Commercialized Biotech/GM Crops In 2017, the accumulated biotech crop area (planted since 1996) surged to a record 2.3 billion hectares or 5.8 billion acres .Of the total number of 24 countries planting biotech crops in 2017, 19 were developing countries and 5 industrialized countries . To put the 2017 global area of biotech crops into context, 189.8 million hectares of biotech crops is equivalent to almost 20% of the total land area of China (956 million hectares) or the USA (937 million hectares) and more than 7 times the land area of the United Kingdom (24.4 million hectares). The 3% increase between 2016 and 2017 is equivalent to 4.7 million hectares or 11.6 million acres.( Source: ISAAA, 2017).
Global Adoption of Biotech Soybean, Maize, Cotton, and Canola The most planted biotech crops in 2017 were soybean, maize, cotton, and canola. Although there was only 3% increase in the planting of biotech soybean, it maintained its high adoption rate of 50% of the global biotech crops or 94.1 million hectares. This area is 80% of the total soybean production worldwide . Biotech maize occupied 59.7 million hectares globally, which was 32% of the global maize production in 2017. A slight decline (1%) in the biotech maize area from 2016 is due to the unfavorable weather conditions in Latin America, low market price, lesser pest incidence, high year-end stocks .
Biotech cotton was planted to 24.1 million hectares in 2017, which indicates a decrease by 8% from 2016. The 8% increase in total biotech cotton area globally was due mainly to the improved global market value and the high adoption rate of insect resistant/herbicide tolerant cotton in 2017. Biotech canola increased by 19% from 8.6 million hectares in 2016 to 10.2 million hectares in 2016. This raise is attributed to the two- digit increases in biotech canola plantings in the USA, Canada, and Australia, addressing the demand for edible oil.
Discussion Questions 1. What are regulations supposed to achieve? 2. With GM crops spreading so quickly, how are we assured of their health and environmental safety? 3. How is genetic engineering (biotechnology) regulated? 4. How do the risks posed by products of biotechnology compare to those posed by conventional technologies? 5. How does biotechnology threaten biosafety? 6. How do different countries regulate products of biotechnology?
Environmental concern And Bio- safety Issues Related to BT gene
 Bio-safety of human and animal health  Labeling GM and non-GM food  Antibiotic resistance genes in GM food  Environmental concerns  Gene transfer  Effects on bio-diversity  Public awareness  Socio-economic issues crops:
1.Safety of source organism and genes a. safety of inserted DNA b. safety of DNA c. safety of antibiotic resistance marker 2.Safety issue of newly developed product a. potential for toxicity (protein product) b. potential of allergencity(protein product) c. safety of any unintended effects 3.Equivalence of composition 4.Retention of nutritional value 5.The human dietary exposure Food safety issues
• With the kind of concerns witnessed among the public, keeping GM and non GM products separately with appropriate labelling , perhaps also through colour codes for illiterate people, may be absolutely necessary. • The related issue is the need for a certification agency specializing in certifying the GM nature of a product. • However, labeling may create confusion among consumers and add to the cost of GM products. Labeling GM and non GM Food
• Another concern is the transfer of antibiotic resistance from the GM food consumed by people into the bacteria inhabiting the human gut, which might result in a disease causing bacterial population to become resistant to antibiotics. • The transgenic developers should continue to remove more rapidly all such markers from GM plants and utilize alternative markers for the selection of new varieties. Resistance to Antibiotics
• Effect of transgenic plants on population dynamics of target and non target pests • Secondary pest problems • Insect sensitivity • Evolution of new insect biotypes • Environmental influence on gene expression • Development of resistance in insect population • Development of resistance to herbicide Environmental Concerns
Direct effects on non-target organisms In May 1999, it was reported that pollen from Bacillus thuringiensis (Bt) insect resistant corn had a negative impact on Monarch butterfly larvae. This report raised concerns and questions about potential risks to Monarchs and perhaps other non- target organisms.
Development of insect resistance The use of Bt crops is that it will lead to the development of insect resistance to Bt •Insect resistance management plans have been developed by government, industry, and scientists to address this issue – These plans include a requirement that every field of insect-resistant crops must have an associated refuge of non-GM crops in order for the insects to develop without selection to the insect resistant varieties.
Gene flow • Accidental cross breeding GMO plants and between traditional pollen Varieties transfer through can contaminate the traditional local varieties with GMO genes resulting in the loss of traditional varieties of the farmers.
Increased weediness •Weediness means the tendency of the plant to spread beyond the field where it was first planted. •There are apprehensions about GM crops becoming weeds. • For example, a salt tolerant GM crop if escapes into marine areas could become a potent weed . •There is also fear about the development of superweeds i.e. a weed that has acquired the herbicide tolerant gene due to genetic Contamination. •With a herbicide tolerance GMO through in field cross breeding to related species or through horizontal gene transfer.
• GM crops could lead to erosion of biodiversity and 'pollute' gene pools of endangered plant species. • The large acreage of elite varieties have pushed and will continue to push the landraces out of cultivation • The potential transfer of a transgene to local flora to and its any possible subsequent impact on specific plant species is thus an issue that needs to be kept in view before commercial release of specific transgenes. Effect on Biodiversity
Consumer response depends on perceptions about risks and benefits of genetically modified foods. In order to maximize the trust, it is essential that relevant and reliable information about the genetically modified food is communicated to the consumers and stake holders. The media, individuals, scientists and administrators, politicians and NGO have the responsibility to educate the people about the benefits of GM foods. Public Attitude
Potential benefits to the consumers and farmers. Due to increasing seed market, the developing countries may get dependent on few suppliers. Countries with effective and efficient technology will obtain and sustain advantage of international market. Reservation of E.U. for acceptance of GM crops. economic issues
Genetic engineering creates novel genetic combinations All GMOs are tested for potential environmental impacts prior to sale •influence on soil and water composition •insect resistance management •gene/trait transfer to weedy relatives •interactions with agricultural environment GMO Crops Have Many Significant Environmental Benefits •Reduced chemical pesticide and herbicide use •More sustainable pest management •Better erosion control through no-till practices fits
Registration of transgenic agricultural organisms
•The Genetically Modified Organisms (GMOs) and products thereof are regulated articles in India in view of potential risks to human health and environment by indiscriminate use under “Rules for Manufacture, Use, Import, Export and Storage of Hazardous Microorganisms/Genetically engineered organisms or cells, 1989 under the EPA (1986)”. • India has ratified the Biosafety Protocol in January 2003 Biosafety Framework Government Commitment
The Cartagena Protocol on Biosafety to the Convention on Biological Diversity has been ratified and signed by 171 parties (Fabruary 2018). According to the Cartagena Protocol, field trials and all other activities involving genetically modified organisms must be regulated and approved by national governments. Regulatory and Approval system for release of GM plant
The European Community (EC) and Development Organization for Economic Cooperation (OECD) WHO/FAO Working Group on Biosafety on the Environment and United Nations Conference Development (UNCED) Codex Alimentarius Commission Agencies of the World
Government of India Department of Biotechnology Ministry of Environment ,forest & Climate Change RDAC RCGM GEAC Institutional Biosafety Committee State Biotechnology Coordination Committee District Level Committee Indian biosafety regulatory framework
for field trials and environmental release of transgenic crops. Institutional Biosafety Committee (IBSC) (Forwarding applications for approval of RCGM) Review Committee on Genetic Manipulation (RCGM) (green house experiments, contained field trails i.e .in –house trial /intial hybrid trial, generation of data on gene stability and expression , biosafetydata) RCGM (approval for conduct of multi-location field trials on selected varieties )/hybrid)and biosafety data) Evaluation of field trails by Monitoring –Cum-Evaluation Committee (MEC) Through SAUs
Genetic Engineering Approval Committee(GEAC) Seed production 10 ha ICAR trials 1St year Large Scale Trials (LST) Large Scale Trials (LST) Seed production 100ha ICAR trials 2St year MEC GEAC (Enviornmental clearance of the event/gene in a given backgrounnd) Material cleared from Enviornmental sensitivity by MoEF/ or otherwise Conti.....
Future Prospects Future envisaged applications of GMOs are diverse and include drugs in food, bananas that produce human vaccines against infectious diseases such as Hepatitis B, metabolically engineered fish that mature more quickly, fruit and nut trees that yield years earlier, and plants that produce new plastics with unique properties. While their practicality or efficacy in commercial production has yet to be fully tested, the next decade may see exponential increases in GM product development as researchers gain increasing access to genomic resources that are applicable to organisms beyond the scope of individual projects. Safety testing of these products will also at the same time be necessary to ensure that the perceived benefits will indeed outweigh the perceived and hidden costs of development.
Biotech crops is not a panacea; but they have the potential to make a substantial contribution in cutting poverty by half, by optimizing crop productivity, which can be achieved by public- private sector partnerships.
• The development of Transgenic plants is the result of integrated application of rDNA technology,Gene transfer methods and Tissue culture techniques. • Transgenic plants are designed to acquire useful quality attributes such as insect resistance, herbicide tolerance, abiotic stress tolerance, disease resistance, high nutritional quality, high yield potential, delayed ripening, enhanced ornamental value, male transgenic crops before sterility, and production of edible vaccines. Biosafety regulations required to assess the safety of its release in to environment. “The controversy about the health safety of Transgenic foods is complex and good science and its communication are required in order to find solutions”
Case studies : 1. Socio-Economic Impact of Bt Cotton — A Case Study of Karnataka V.R. Kiresur and Manjunath Ichangi University of Agricultural Sciences, Dharwad-580 005, Karnataka The performance Bt technology and its impact on farming community have been assessed in northern Karnataka based mainly on primary data processed using production functions, decomposition analysis and logit model. On an average, per farm area under Bt cotton was 2.21 ha, accounting for 66 per cent of the total landholding. With a yield of 24 q/ha, Bt cotton has registered 31 per cent higher yield and 151 per cent higher net return over non-Bt, the net additional benefit being ` 18429/ha. The non-Bt cotton farmers use chemical fertilizers, organic manures and bullock labour excessively which result in a lower net returns. Technology has been found the major contributor to the total productivity difference between Bt and nonBt cottons. Seed cost, yield of Bt cotton and cost of plant protection have been found to greatly influence the probability of adoption of Bt cotton.
Non-availability of quality seeds and in required quantity have been identified as the most important factors constraining Bt technology adoption. The impact of Bt cotton, as perceived by the farmers, has been in terms of enhanced yield; reduced pest and disease incidence; increased income, employment, education and standard of living; and reduced health risk. To foster adoption, availability of quality and quantity of Bt cotton seed to farmers needs greater attention of development agencies, while researchers’ attention is called for incorporating resistance/ tolerance to Spodoptera and pink bollworms. 2.Genetically Modified Crops in India: The Bt Brinjal Controversy The Genetic Engineering Approval Committee (GEAC)5 announced approval for large scale field trials for Bt brinjal6 in September 2007, and probably its commercialization by early 2009. It also cleared proposals for biosafety studies for other food crops such as okra (lady's finger), rice, and tomatoes. In February 2008, the apex legislative body in India, the Supreme Court, revoked the ban it had earlier placed on the approval of large scale field trials of transgenic crops. Following this announcement, Bt brinjal became a hotly debated topic among activists, scientists, farmers and Multi National Companies (MNCs).
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transgenic breeding

  • 1.
    Submitted By Chanda kumari Submittedto Dr. Anant Department of Genetics and Plant Breeding Lovely Professional University, Punjab AN ASSIGNMENT on Transgenics
  • 2.
    What is atransgenic? Transgene Exotic gene added to a species through recombinant DNA technology The organism that develop after successful transformation - transgenic BT COTTON GOLDEN RICE FLAVR SAVR TOMATO  Introduction
  • 3.
    What is TransgenicBreeding ? Genetic improvement of crop plants, domestic animals, microbes through Biotechnology. What is Transgenics ? A genotype developed by the process ofgenetic engineering. Or A genotype containing foreign gene or modified geneof different species transferred by process of genetic engineering. What is Transgene ? Foreign gene or modified gene of any species which is used for development of transgenics. These may be from related wild species, microbes (Bacteria, viruses and fungi) or unrelated species.
  • 4.
    Fig 1: Adiagram re-drawn from Harlan and de Wet (1971) to include a description of gene pools as they relate to the use of transgenes . Circles are representations of the primary- (GP-1), secondary- (GP-2), tertiary- (GP-3), and quaternary-gene pools (GP-4)
  • 5.
    Global area (MillionHectares) of Biotech crops, 1996-2016 by Country and Mega-Countries Source: ISAAA, 2016
  • 6.
    Global Adoption Rates(%) for Principal Biotech Crops 2017 (Million Hectares) Trait Distribution in Approved Events, 1992-2016
  • 7.
    Status of BiosafetyResearch Trails of Biotech Crops in India, 2016 Source: MOEF&CC, 2016, Analyzed by ISAAA,2016
  • 8.
    Why Create Transgenic Plants? 1.Improve agricultural value of plant increase yield (herbicide-resistance, pest- resistance) enhance nutrition enhance taste 2. Plants can produce proteins for human needs (pharmaceutics) 3. Modified plants can be used to study effects of genes An entire plant can be regenerated from a single cell ***TOTIPOTENCY*** No separation of germ and somatic cells
  • 9.
    Overview of TheProcess  There are five major steps involved in genetically engineering plants. These are DNA isolation, single gene cloning, gene designing, cell transformation, and backcross breeding. • DNA is extracted from an organism that has the desired trait. • The desired gene is located and copied. • The gene is inserted into a single plant cell using a transformation method. If the transgene successfully lands in the cells nucleus and is incorporated into one of the chromosomes, then the trait that it codes for will be expressed in the cell's offspring. • The cell multiplies and grows a new plant that contains the transgene in all of its cells. • Through backcross breeding the transgenic plant is crossed with a plant from a high yielding line. The resulting hybrids are the genetically modified plants that can enter the marketplace.
  • 10.
    General procedure ofrecombinant DNA technology
  • 11.
    APPLICATION OF TRANSGENICPLANTS • Resistance to biotic and abiotic stresses
  • 12.
    Improving crop yieldand nutritional value
  • 13.
    Help in alleviatingpoverty and hunger
  • 14.
    Contribute to food,feed and fiber security
  • 15.
    Transgenic plants asbioreactors for recombinant proteins
  • 17.
  • 18.
  • 19.
    GENE CONSTRUCT A typicalplant gene Promoters /enhancers Reporter genes
  • 20.
    METHODS FOR PLANTGENE TRANSFER INDIRECT METHODS (VECTOR-BASED) DIRECT METHODS (VECTOR-LESS ) • Agrobacterium mediated transfromation • Bacterial transformation • Conjugation • Phage transduction • Retroviral transduction • Transposition IN PLANTA TRANSFORMATION Physical methods • Particle bombardment • Electroporation • Microinjection • Liposome mediated DNA transfer • Silicon Carbide fibre mediated DNA transfer Chemical method • PEG-mediated DNA transfer • Floral Dip • Vacuum infiltration • Agro injection
  • 23.
    Figure: A biolisticmicroprojectile gun. Source: http://en.wikipedia.org/wiki/Gene_gun (cc) DNA- or RNA-coated gold/tungsten particles are loaded into the gun and you pull the trigger.
  • 24.
    PARTICLE BOMBARDMENT (BIOLISTICS) The micro projectile bombardment method was initially named as biolistics by its inventor Sanford (1988).  Biolistics is a combination of biological and ballistics. There are other names for this technique- particle gun, gene gun, bio blaster.  Foreign DNA containing the genes to be transferred is coated onto the surface of minute gold or tungsten particles (1-3 micrometers) and bombarded onto the target tissue or cells using a particle gun.  Two types of plant tissue are commonly used for particle bombardment- Primary explants and the proliferating embryonic tissues.  Successfully used for the transformation of many cereals, e.g. rice, wheat, maize.  A commercially produced particle bombardment apparatus namely PDS-1000/HE is widely used these days.
  • 25.
    Advantages : i. Genetransfer can be efficiently done in organized tissues. ii. Different species of plants can be used to develop transgenic plants. Limitations : iii. i. The major complication is the production of high transgene copy number. This may result in instability of transgene expression due to gene silencing. ii. The target tissue may often get damaged due to lack of control of bombardment velocity. Sometimes, undesirable chimeric plants may be regenerated.
  • 27.
    VECTORS FOR THEPRODUCTION OF TRANSGENIC PLANTS Plasmid vectors • Ti plasmid • co integrative vector • Binary vectors Plant virus vectors
  • 28.
    Bacillus Shigetane Ishiwatari (1901),first isolated thuringiensis. Bt is commonly abbreviated as Bacillus thuringiensis,is a gram-positive, facultative aerobic, rod-like, motile and sporulating bacterium. Bt is a naturally-occurring soil borne bacterium that is found worldwide Ubiquitous in nature. Produces crystals of endotoxin (Cry protien or delta toxin) - toxic to insect mainly in their larval stage, thus they act as insecticides. Bacillus thuringiensis
  • 29.
     These crystalproteins (Cry proteins) are insect stomach poisons.  Insects stop feeding within two hours of a first bite and, if enough toxin is eaten, die within two or three days  Important biological insect control agent.  Bt crystals, sometimes referred as insecticidal crystal proteins (ICP), are protein crystals formed during sporulation in some Bt strains coded by cry genes. Conti.....
  • 30.
    • 1. Ingestion •2. Solublization & proteolytic activation • 3. Binding to target site • 4. Formation of toxic lesions HOW Bt WORKS ?
  • 32.
    Mode of Actionof Bt Toxin in Insect Gut
  • 33.
    Development of GMCrop/ Transgenics  Identify gene(s) giving a desired trait  Make copies of the gene  Transfer to plant tissue  Regenerate plants  Lab analysis and safety testing  Development of a variety  Field tests  Approval by Government agencies  Commercialization  Monitoring of efficacy and safety
  • 34.
    Schematic Diagram ofBt Crop Improvement
  • 35.
    Delivering the Geneto the Plant •Transformation cassettes are developed in the lab • They are then introduced into a plant •Two major delivery methods • Agrobacterium Tissue culture required to generate transgenic plants • Gene Gun
  • 37.
  • 38.
    Cell Culture andSomatic Embryogenesis, a Means to Obtain TransgenicPlants Scheiden and Schwann made a critical observation, that plants possess a remarkable ability to generate free-living cells from plant tissues. Plant cell and tissue culture using sterile technique and in vitro (within glass) conditions are key elements to obtaining transgenic crop plants. Somatic Embryogenesis Totipotentcy: plant cell or cells are able to live independently, and also possess the ability to regenerate into a whole plant under the right environmental conditions. The first methodology of generating plants from cell culture is through the process of somatic embryo formation, whereby somatic cells (those not involved in sexual reproduction), produce an embryo similar to one produced by zygotic embryogenesis it is called somatic embryogenesis. Somatic embryos formed with root and shoot apical meristems are termed a bipolar embryo, and germinate into whole plants (Parrott, 2000). Organogenesis The second methodology of regenerating single cells into whole plants is termed organogenesis, where a meristematic cell from a root or shoot primordiums used to form organs (e.g. shoots, leaves or roots); these recovered organs can then be cultured into whole plants. Cell culture and transformation
  • 39.
    Transgenic Crops: Development Objectives First-generationGM crops Herbicide resistance:- Corn,Soybean,rice,and Sugar beet Insect Pest resistance:- Corn, rice tomato and potato Viral resistance:- Papaya, Squash and potato Slow ripening and softening- Tomato and melon Improved oil quality -Canola and soybean Male sterility - Canola and corn
  • 40.
    Second generation ofGM crops Increased levels of protein Modified and healthier fats Modified carbohydrates Improved flavor characteristics  Increased levels of micronutrients Third generation of GM crops Resistance to abiotic stress “Pharmaplants” Conti..
  • 41.
    Transgenic Breeding forInsect Resistance Crop Gene inserte d Resistance to Vector Achievement Year 1) Cotton Cry gene comple x Bollworm complex Agrobacteriu m tumefacience 1) 1 st Bt cotton in world by Monsanto Ltd. USA. 2) 1st Bt cotton in india - 1996 by MAHYCO - Monsanto Ltd. 1987 1996 2) Brinjal Cry 1 Ab Fruit and shoot borer Agrobacteriu m tumefacience 1) 1 st transgenic (Bt) Brinjal in world ( USA) 2004 3) Cabbage Cry gene comple x Cabbage worm and cabbage looper Agrobacteriu m tumefacience 1) 1 st transgenic (Bt) Cabbage in world (USA) 2002 4)Maize Cry gene complex European Corn Borer Agrobacterium tumefacience 1) 1st Transgenic maize in world is Maximizer (Spain) by Company Ciba Geigy 1995 5)Okra Cry 1 Ab Fruit and shoot borer Agrobacteriu m 1) 1 st Transgenic okra in world by U.S.A. 2002
  • 42.
    Crop Gene inserted Resistance to Vector AchievementYear 6) Potato Cry-gene complex Colorado potato beetle Agrobacteriu m tumefacience First transgenic potato in world – Monsanto Ltd. U.S.A. 1995 7) Apple Cry-1-Ac Codling moth Agrobacteriu m tumefacience First transgenic Apple in world by U.S.A. 2000 8) Soybean Cry-1-Ac Leaf eating catterpilla r Agrobacteriu m tumefacience First transgenic Soybean in world – Monsanto Ltd. U.S.A. 1996 9) Sugar cane Cry-1-Ab Stem borer Agrobacterium tumefacience First transgenic Sugar cane in world – U.S.A. DNA plant technology company 1997 10) Tomato Cry-1-Ac Tobacco horn worm Agrobacteriu m tumefacience First transgenic Tomato in world – U.S.A by Calgene company 1987 11)Tobacco Trypsin inhibitor gene from cowpea Leaf eating catterpilla r Agrobacteriu m tumefacience First transgenic tobacco resistant to leaf eating catterpillar was developed in USA. 2001. Source – Esseentials of Plant Breeding
  • 43.
    Transgenic breeding forkeeping quality Crop Gene inserted Traits Vector Achievement 1) Tomato - a) Delay ripening a) Agrobacteriu m tumefacience b) Antisense RNA technology c)Agrobacteriu m tumefacience a) Variety : Endless summer by DNA plant technolog y company 1995 b) Delay fruit softening b) Flavr Savr b y Calgene company in 1994 c) Thicker skin and Alter c) By Zeneca compan
  • 44.
    Crop Gene insertedTraits Vector Achievement 2) Apple ACC oxidase gene Delay ripening Agrobacteriu m tumefacience In scion cultivar ROYAL GALA is developed 3) Banana ACC gene Delay ripening Agrobacteriu m tumefacience Cavendish 1 4) Mango Rol C gene Storage and delay ripening Agrobacteriu m tumefacience Golden yellow ( Source - AGROBIAS Newsletter. 2006).
  • 45.
    Transgenic breeding fordisease resistance Crop Gene inserted Resistance to Vector Achievement 1) Potato PLRV-R PVX, PVY, PLRV Agrobacterium tumefacience First transgenic potato in world by – Monsanto Ltd. U.S.A. 2000 2) Cumcumber GUS and Markar gene NPT II CMV, ZYMV, WMV 2 Agrobacteriu m tumefacience First resistance variety Beit alpha MR 3) Faba bean Chitinase gene from Seratica marcescence Chocolate spot disease Plasmid First resistance variety in 2002 4) Banana FR gene Panama wilt Plasmid First resistance variety isCavendish 5) Papaya PRVR gene Papaya ring spot virus Plasmid First resistance variety is Sunup and second variety is Rainbow in 1999
  • 46.
    Transgenic breeding forHerbicide Resistance Crop Gene transfer resistance to Vector Achivement 1.Rice Bar chimeric gene Hygromycine Plasmid Oryza sativa cv. IR 72 2.Nilgiri (Eucalyptus) Cry 3A Glufosinate ammonium(Liberty@ 6 l/ha) Agrobacteriu m tumefacience Variety-Ecofriend 3.Sugarcane PPTR( phospinothricin e resistant gene) Phosphinothricine Agrobacteriu m tumefacience I st herbicide resistant sugarcane was developed in USA. 4.Cotton Phosphinothric acetyl transeferase All non selective herbicides Particle gun bombardmen t method I st variety was releasedIn year 2000.
  • 47.
    Transgenic breeding foradding Male sterility Crop Gene inserted Source of gene 1.Rapeseed mustard msi (male sterility iducing gene) Bacillus amyloliquefacien ce Source – Essentials of Plant Breeding , Transgenic breeding for drought resistance 1.Drought resistance in sorghum Gene transformed mlt D gene Vector Agrobacterium tumefacience Developed at CRIDA, Hyderabad Number of transgenics released 14.
  • 48.
    2. Drought resistancein Wheat 1. Crop American bread wheat 2.Genes transformed mlt D and HVA 1. 3.Source of gene Osmotin gene complex from Atriplex numularia 4. By Microprojectile bombardment system Transgenic breeding for changing morphological character 1. Crop Petunia 2. Character modified Orange flower colour 3.Gene transferred Dihydroflavanal 4 reductase (FR) gene. 4.Source of gene Zea mays L. (Corn) Original Petunia Modified Petunia
  • 49.
    Insect resistant cotton– Bt toxin kills the cotton boll worm  transgene = Bt protein Insect resistant corn – Bt toxin kills the European corn borer  transgene = Bt protein Agriculture Transgenics On the Market
  • 50.
    Herbicide resistant crops Now: soybean, corn, canola transgene = modified EPSP synthase or phosphinothricin-N-acetyltransferase Virus resistant papaya • transgene = virus coat protein
  • 51.
    Next Generation ofAg Biotech Products Golden Rice – increased Vitamin A content transgene = three pathway enzymes Sunflower – white mold resistance transgene = oxalate oxidase from wheat
  • 52.
    Global Status ofCommercialized Biotech/GM Crops In 2017, the accumulated biotech crop area (planted since 1996) surged to a record 2.3 billion hectares or 5.8 billion acres .Of the total number of 24 countries planting biotech crops in 2017, 19 were developing countries and 5 industrialized countries . To put the 2017 global area of biotech crops into context, 189.8 million hectares of biotech crops is equivalent to almost 20% of the total land area of China (956 million hectares) or the USA (937 million hectares) and more than 7 times the land area of the United Kingdom (24.4 million hectares). The 3% increase between 2016 and 2017 is equivalent to 4.7 million hectares or 11.6 million acres.( Source: ISAAA, 2017).
  • 53.
    Global Adoption ofBiotech Soybean, Maize, Cotton, and Canola The most planted biotech crops in 2017 were soybean, maize, cotton, and canola. Although there was only 3% increase in the planting of biotech soybean, it maintained its high adoption rate of 50% of the global biotech crops or 94.1 million hectares. This area is 80% of the total soybean production worldwide . Biotech maize occupied 59.7 million hectares globally, which was 32% of the global maize production in 2017. A slight decline (1%) in the biotech maize area from 2016 is due to the unfavorable weather conditions in Latin America, low market price, lesser pest incidence, high year-end stocks .
  • 54.
    Biotech cotton wasplanted to 24.1 million hectares in 2017, which indicates a decrease by 8% from 2016. The 8% increase in total biotech cotton area globally was due mainly to the improved global market value and the high adoption rate of insect resistant/herbicide tolerant cotton in 2017. Biotech canola increased by 19% from 8.6 million hectares in 2016 to 10.2 million hectares in 2016. This raise is attributed to the two- digit increases in biotech canola plantings in the USA, Canada, and Australia, addressing the demand for edible oil.
  • 55.
    Discussion Questions 1. Whatare regulations supposed to achieve? 2. With GM crops spreading so quickly, how are we assured of their health and environmental safety? 3. How is genetic engineering (biotechnology) regulated? 4. How do the risks posed by products of biotechnology compare to those posed by conventional technologies? 5. How does biotechnology threaten biosafety? 6. How do different countries regulate products of biotechnology?
  • 56.
    Environmental concern And Bio-safety Issues Related to BT gene
  • 57.
     Bio-safety ofhuman and animal health  Labeling GM and non-GM food  Antibiotic resistance genes in GM food  Environmental concerns  Gene transfer  Effects on bio-diversity  Public awareness  Socio-economic issues crops:
  • 58.
    1.Safety of sourceorganism and genes a. safety of inserted DNA b. safety of DNA c. safety of antibiotic resistance marker 2.Safety issue of newly developed product a. potential for toxicity (protein product) b. potential of allergencity(protein product) c. safety of any unintended effects 3.Equivalence of composition 4.Retention of nutritional value 5.The human dietary exposure Food safety issues
  • 59.
    • With thekind of concerns witnessed among the public, keeping GM and non GM products separately with appropriate labelling , perhaps also through colour codes for illiterate people, may be absolutely necessary. • The related issue is the need for a certification agency specializing in certifying the GM nature of a product. • However, labeling may create confusion among consumers and add to the cost of GM products. Labeling GM and non GM Food
  • 60.
    • Another concernis the transfer of antibiotic resistance from the GM food consumed by people into the bacteria inhabiting the human gut, which might result in a disease causing bacterial population to become resistant to antibiotics. • The transgenic developers should continue to remove more rapidly all such markers from GM plants and utilize alternative markers for the selection of new varieties. Resistance to Antibiotics
  • 61.
    • Effect oftransgenic plants on population dynamics of target and non target pests • Secondary pest problems • Insect sensitivity • Evolution of new insect biotypes • Environmental influence on gene expression • Development of resistance in insect population • Development of resistance to herbicide Environmental Concerns
  • 63.
    Direct effects onnon-target organisms In May 1999, it was reported that pollen from Bacillus thuringiensis (Bt) insect resistant corn had a negative impact on Monarch butterfly larvae. This report raised concerns and questions about potential risks to Monarchs and perhaps other non- target organisms.
  • 64.
    Development of insectresistance The use of Bt crops is that it will lead to the development of insect resistance to Bt •Insect resistance management plans have been developed by government, industry, and scientists to address this issue – These plans include a requirement that every field of insect-resistant crops must have an associated refuge of non-GM crops in order for the insects to develop without selection to the insect resistant varieties.
  • 65.
    Gene flow • Accidentalcross breeding GMO plants and between traditional pollen Varieties transfer through can contaminate the traditional local varieties with GMO genes resulting in the loss of traditional varieties of the farmers.
  • 66.
    Increased weediness •Weediness meansthe tendency of the plant to spread beyond the field where it was first planted. •There are apprehensions about GM crops becoming weeds. • For example, a salt tolerant GM crop if escapes into marine areas could become a potent weed . •There is also fear about the development of superweeds i.e. a weed that has acquired the herbicide tolerant gene due to genetic Contamination. •With a herbicide tolerance GMO through in field cross breeding to related species or through horizontal gene transfer.
  • 67.
    • GM cropscould lead to erosion of biodiversity and 'pollute' gene pools of endangered plant species. • The large acreage of elite varieties have pushed and will continue to push the landraces out of cultivation • The potential transfer of a transgene to local flora to and its any possible subsequent impact on specific plant species is thus an issue that needs to be kept in view before commercial release of specific transgenes. Effect on Biodiversity
  • 68.
    Consumer response dependson perceptions about risks and benefits of genetically modified foods. In order to maximize the trust, it is essential that relevant and reliable information about the genetically modified food is communicated to the consumers and stake holders. The media, individuals, scientists and administrators, politicians and NGO have the responsibility to educate the people about the benefits of GM foods. Public Attitude
  • 69.
    Potential benefits tothe consumers and farmers. Due to increasing seed market, the developing countries may get dependent on few suppliers. Countries with effective and efficient technology will obtain and sustain advantage of international market. Reservation of E.U. for acceptance of GM crops. economic issues
  • 70.
    Genetic engineering createsnovel genetic combinations All GMOs are tested for potential environmental impacts prior to sale •influence on soil and water composition •insect resistance management •gene/trait transfer to weedy relatives •interactions with agricultural environment GMO Crops Have Many Significant Environmental Benefits •Reduced chemical pesticide and herbicide use •More sustainable pest management •Better erosion control through no-till practices fits
  • 71.
    Registration of transgenicagricultural organisms
  • 72.
    •The Genetically ModifiedOrganisms (GMOs) and products thereof are regulated articles in India in view of potential risks to human health and environment by indiscriminate use under “Rules for Manufacture, Use, Import, Export and Storage of Hazardous Microorganisms/Genetically engineered organisms or cells, 1989 under the EPA (1986)”. • India has ratified the Biosafety Protocol in January 2003 Biosafety Framework Government Commitment
  • 73.
    The Cartagena Protocolon Biosafety to the Convention on Biological Diversity has been ratified and signed by 171 parties (Fabruary 2018). According to the Cartagena Protocol, field trials and all other activities involving genetically modified organisms must be regulated and approved by national governments. Regulatory and Approval system for release of GM plant
  • 74.
    The European Community(EC) and Development Organization for Economic Cooperation (OECD) WHO/FAO Working Group on Biosafety on the Environment and United Nations Conference Development (UNCED) Codex Alimentarius Commission Agencies of the World
  • 75.
    Government of India Departmentof Biotechnology Ministry of Environment ,forest & Climate Change RDAC RCGM GEAC Institutional Biosafety Committee State Biotechnology Coordination Committee District Level Committee Indian biosafety regulatory framework
  • 76.
    for field trialsand environmental release of transgenic crops. Institutional Biosafety Committee (IBSC) (Forwarding applications for approval of RCGM) Review Committee on Genetic Manipulation (RCGM) (green house experiments, contained field trails i.e .in –house trial /intial hybrid trial, generation of data on gene stability and expression , biosafetydata) RCGM (approval for conduct of multi-location field trials on selected varieties )/hybrid)and biosafety data) Evaluation of field trails by Monitoring –Cum-Evaluation Committee (MEC) Through SAUs
  • 77.
    Genetic Engineering ApprovalCommittee(GEAC) Seed production 10 ha ICAR trials 1St year Large Scale Trials (LST) Large Scale Trials (LST) Seed production 100ha ICAR trials 2St year MEC GEAC (Enviornmental clearance of the event/gene in a given backgrounnd) Material cleared from Enviornmental sensitivity by MoEF/ or otherwise Conti.....
  • 78.
    Future Prospects Future envisaged applicationsof GMOs are diverse and include drugs in food, bananas that produce human vaccines against infectious diseases such as Hepatitis B, metabolically engineered fish that mature more quickly, fruit and nut trees that yield years earlier, and plants that produce new plastics with unique properties. While their practicality or efficacy in commercial production has yet to be fully tested, the next decade may see exponential increases in GM product development as researchers gain increasing access to genomic resources that are applicable to organisms beyond the scope of individual projects. Safety testing of these products will also at the same time be necessary to ensure that the perceived benefits will indeed outweigh the perceived and hidden costs of development.
  • 79.
    Biotech crops isnot a panacea; but they have the potential to make a substantial contribution in cutting poverty by half, by optimizing crop productivity, which can be achieved by public- private sector partnerships.
  • 80.
    • The developmentof Transgenic plants is the result of integrated application of rDNA technology,Gene transfer methods and Tissue culture techniques. • Transgenic plants are designed to acquire useful quality attributes such as insect resistance, herbicide tolerance, abiotic stress tolerance, disease resistance, high nutritional quality, high yield potential, delayed ripening, enhanced ornamental value, male transgenic crops before sterility, and production of edible vaccines. Biosafety regulations required to assess the safety of its release in to environment. “The controversy about the health safety of Transgenic foods is complex and good science and its communication are required in order to find solutions”
  • 81.
    Case studies : 1.Socio-Economic Impact of Bt Cotton — A Case Study of Karnataka V.R. Kiresur and Manjunath Ichangi University of Agricultural Sciences, Dharwad-580 005, Karnataka The performance Bt technology and its impact on farming community have been assessed in northern Karnataka based mainly on primary data processed using production functions, decomposition analysis and logit model. On an average, per farm area under Bt cotton was 2.21 ha, accounting for 66 per cent of the total landholding. With a yield of 24 q/ha, Bt cotton has registered 31 per cent higher yield and 151 per cent higher net return over non-Bt, the net additional benefit being ` 18429/ha. The non-Bt cotton farmers use chemical fertilizers, organic manures and bullock labour excessively which result in a lower net returns. Technology has been found the major contributor to the total productivity difference between Bt and nonBt cottons. Seed cost, yield of Bt cotton and cost of plant protection have been found to greatly influence the probability of adoption of Bt cotton.
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    Non-availability of qualityseeds and in required quantity have been identified as the most important factors constraining Bt technology adoption. The impact of Bt cotton, as perceived by the farmers, has been in terms of enhanced yield; reduced pest and disease incidence; increased income, employment, education and standard of living; and reduced health risk. To foster adoption, availability of quality and quantity of Bt cotton seed to farmers needs greater attention of development agencies, while researchers’ attention is called for incorporating resistance/ tolerance to Spodoptera and pink bollworms. 2.Genetically Modified Crops in India: The Bt Brinjal Controversy The Genetic Engineering Approval Committee (GEAC)5 announced approval for large scale field trials for Bt brinjal6 in September 2007, and probably its commercialization by early 2009. It also cleared proposals for biosafety studies for other food crops such as okra (lady's finger), rice, and tomatoes. In February 2008, the apex legislative body in India, the Supreme Court, revoked the ban it had earlier placed on the approval of large scale field trials of transgenic crops. Following this announcement, Bt brinjal became a hotly debated topic among activists, scientists, farmers and Multi National Companies (MNCs).
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