This document provides an overview of immunohematology, focusing on blood group systems. It discusses key concepts including antigens, antibodies, and the major blood group systems like ABO and Rh. The ABO system is described in detail, including the antigens (A, B, H), antibodies (anti-A, anti-B), inheritance patterns, common subtypes, frequencies in different populations, and routine testing methods involving direct and indirect agglutination. Causes of discrepancies in ABO testing are also reviewed. Overall, the document serves as an introduction to immunohematology and blood group systems from an immunological perspective.
This document discusses the ABO blood group system. It notes that there are over 20 known blood group systems that are genetically determined. The ABO and Rh systems are most important for blood transfusions. The ABO system involves antigens on red blood cells and corresponding antibodies in plasma. People are categorized into one of the main blood groups - A, B, AB, or O - depending on which antigens are present on their red blood cells and which antibodies are present in their plasma. The exact genetic basis and inheritance of the ABO system is also described.
Rh typing and its technique , BLOOD TYPING , Rhesus (Rh) typing , procedures of rh typing, process of Rh typing, Test limitations, Sources of Error in Rh Antigen Typing, False positive reactions' reason, False negative reactions' reasons
Pretransfusion testing final- ab screening - NAGLAA MAKRAM Naglaa Makram
1. Antibody screening tests patient serum against reagent red blood cells to detect unexpected antibodies that could destroy transfused donor cells.
2. Screening cells must contain many common antigens and include some cells with homozygous antigen expression to detect weakly reacting antibodies.
3. A positive antibody screen requires antibody identification testing to determine the antibody specificity so that antigen-negative blood can be transfused.
The document discusses compatibility testing protocols for blood transfusions. It describes how compatibility testing includes ABO and Rh grouping of donor and recipient samples, screening for unexpected antibodies, and a cross-match. Proper identification of donor and recipient samples is critical to avoid errors. The purpose is to select appropriately compatible blood and ensure the best results for the transfusion by preventing hemolysis or antibody-mediated destruction of transfused red blood cells.
A woman had an anaphylactic reaction during a blood transfusion minutes after it started, with symptoms of flushing, chest tightness and difficulty breathing. Epinephrine was administered with good effect. The most likely cause was the woman being IgA-deficient and having antibodies against IgA in the donor blood.
A woman developed a fever and jaundice after a blood transfusion during surgery. Her hemoglobin had dropped unexpectedly. The most likely cause was recipient antibodies against donor red blood cell antigens not detected on the pre-transfusion testing, causing a delayed hemolytic reaction.
A man receiving a blood transfusion developed a fever within 15 minutes. Tests found no evidence of hemolysis.
The ABO blood group system was discovered in 1900 by Karl Landsteiner, who identified three main blood types: A, B, and O. The fourth type, AB, was discovered later. People have antigens on their red blood cells and corresponding antibodies in their plasma. Blood type is inherited and determines compatibility for transfusions. Type O negative blood can be donated to all recipients, while type AB positive can receive from all donors.
This document discusses the history and science of blood types, including the discovery of antigens and antibodies in blood and their role in determining blood compatibility. It specifically examines the rare Bombay blood type, which can receive blood from any type but can only donate to other Bombay individuals. The document stresses the importance of blood donation and registration to help those with rare blood types.
The LE cell demonstration document describes the LE cell, which is a neutrophil that has phagocytosed nuclear material coated with antinuclear antibodies, a characteristic of lupus erythematosus. It discusses several methods for demonstrating LE cells in blood samples, including using clotted blood, defibrinated blood, or the rotary method. The rotary method involves adding glass beads to heparinized blood and rotating at 50rpm for 30 minutes at 37 degrees Celsius before preparing buffy coat smears to identify LE cells.
This document discusses the ABO blood group system. It notes that there are over 20 known blood group systems that are genetically determined. The ABO and Rh systems are most important for blood transfusions. The ABO system involves antigens on red blood cells and corresponding antibodies in plasma. People are categorized into one of the main blood groups - A, B, AB, or O - depending on which antigens are present on their red blood cells and which antibodies are present in their plasma. The exact genetic basis and inheritance of the ABO system is also described.
Rh typing and its technique , BLOOD TYPING , Rhesus (Rh) typing , procedures of rh typing, process of Rh typing, Test limitations, Sources of Error in Rh Antigen Typing, False positive reactions' reason, False negative reactions' reasons
Pretransfusion testing final- ab screening - NAGLAA MAKRAM Naglaa Makram
1. Antibody screening tests patient serum against reagent red blood cells to detect unexpected antibodies that could destroy transfused donor cells.
2. Screening cells must contain many common antigens and include some cells with homozygous antigen expression to detect weakly reacting antibodies.
3. A positive antibody screen requires antibody identification testing to determine the antibody specificity so that antigen-negative blood can be transfused.
The document discusses compatibility testing protocols for blood transfusions. It describes how compatibility testing includes ABO and Rh grouping of donor and recipient samples, screening for unexpected antibodies, and a cross-match. Proper identification of donor and recipient samples is critical to avoid errors. The purpose is to select appropriately compatible blood and ensure the best results for the transfusion by preventing hemolysis or antibody-mediated destruction of transfused red blood cells.
A woman had an anaphylactic reaction during a blood transfusion minutes after it started, with symptoms of flushing, chest tightness and difficulty breathing. Epinephrine was administered with good effect. The most likely cause was the woman being IgA-deficient and having antibodies against IgA in the donor blood.
A woman developed a fever and jaundice after a blood transfusion during surgery. Her hemoglobin had dropped unexpectedly. The most likely cause was recipient antibodies against donor red blood cell antigens not detected on the pre-transfusion testing, causing a delayed hemolytic reaction.
A man receiving a blood transfusion developed a fever within 15 minutes. Tests found no evidence of hemolysis.
The ABO blood group system was discovered in 1900 by Karl Landsteiner, who identified three main blood types: A, B, and O. The fourth type, AB, was discovered later. People have antigens on their red blood cells and corresponding antibodies in their plasma. Blood type is inherited and determines compatibility for transfusions. Type O negative blood can be donated to all recipients, while type AB positive can receive from all donors.
This document discusses the history and science of blood types, including the discovery of antigens and antibodies in blood and their role in determining blood compatibility. It specifically examines the rare Bombay blood type, which can receive blood from any type but can only donate to other Bombay individuals. The document stresses the importance of blood donation and registration to help those with rare blood types.
The LE cell demonstration document describes the LE cell, which is a neutrophil that has phagocytosed nuclear material coated with antinuclear antibodies, a characteristic of lupus erythematosus. It discusses several methods for demonstrating LE cells in blood samples, including using clotted blood, defibrinated blood, or the rotary method. The rotary method involves adding glass beads to heparinized blood and rotating at 50rpm for 30 minutes at 37 degrees Celsius before preparing buffy coat smears to identify LE cells.
This document discusses blood group systems, specifically ABO and Rh blood groups. It provides details on:
- The antigens found on red blood cell membranes that determine blood type
- Landsteiner's discovery of the ABO blood group system in 1900 and the four main blood types (A, B, AB, and O)
- The antigens and antibodies present in each blood type
- Rh blood group system including the Rho(D) antigen and typing only for Rho(D) to determine Rh status
- Techniques for blood typing including tube, slide, microplate, and newer gel/cassette methods
- Interpreting and resolving discrepancies in blood typing results
This document provides an overview of basic principles of immunohematology. It defines key terms like antigen and antibody. It describes the characteristics of antigens and factors that contribute to antigen immunogenicity. It also discusses the different types of immunoglobulins involved in blood group antibodies, and the differences between naturally occurring versus immune antibodies. Finally, it explains the stages of antigen-antibody reactions including sensitization and agglutination, and factors that can influence these reactions.
The document discusses the ABO blood group system. Some key points:
- Karl Landsteiner discovered the ABO blood group system in 1900-1901. It identifies four main blood groups: A, B, AB, and O.
- The presence or absence of A and B antigens on red blood cells determines an individual's blood group. Those without A or B antigens are group O.
- Anti-A and anti-B antibodies are naturally present in people's blood, developing after exposure to environmental antigens. These antibodies can cause hemolytic transfusion reactions if incompatible blood is transfused.
- The ABO blood groups are determined genetically based on inheritance of A, B, or O alleles. The A
A blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs). These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system.
This document discusses various techniques used in blood banking and transfusion medicine, including:
1. Pretransfusion testing involves ABO/Rh typing, antibody screening, and crossmatching to select compatible blood and prevent hemolytic transfusion reactions.
2. Antibody identification uses a panel of red blood cells to identify the specific antibody in a patient's serum through various testing phases including immediate spin, LISS incubation, and antiglobulin.
3. Special techniques like elution, hemagglutination inhibition, and titration are used to further characterize antibodies or quantify their concentration.
The document summarizes key information about the rare Bombay blood group:
- The Bombay blood group lacks the H, A, and B antigens found on red blood cells, and those with this group have antibodies against these antigens in their plasma. It was first discovered in Bombay, India and is more common in some parts of India.
- Those with the Bombay blood group must receive blood from other Bombay donors, as their red blood cells will hemolyze if transfused with blood containing the H, A, or B antigens. Testing for this rare group involves reverse grouping or detection of H antibodies.
- It is important for those with the Bombay blood group to register with blood banks
This document provides an overview of antibody identification in blood banking. It discusses the key steps in performing an antibody panel, including using a panel of known red blood cells to test against a patient's unknown serum. The goal is to identify any unexpected antibodies in the patient's serum. It also covers interpreting panel results, such as ruling out non-reactive antigens and looking for a matching antigen pattern. Techniques for identifying multiple antibodies like selected cells, neutralization, and chemical treatments are also outlined.
This document discusses thalassemias and hemoglobinopathies. It begins by outlining the objectives and topics to be covered, which include pathophysiology, classification, laboratory testing correlations, and treatment. The main types and classifications of alpha and beta thalassemias are then defined. Characteristics, demographics, genetics, terminology and classifications of hemoglobinopathies are also introduced. Details on primary and secondary laboratory investigations and correlations are provided. The document closes with descriptions of treatment approaches and management considerations for thalassemias and hemoglobinopathies.
Gel technology provides an innovative approach to performing various tests in immunohaematology with improved sensitivity and specificity compared to conventional tube techniques. It involves centrifuging red blood cells through a gel column where agglutination reactions occur. The distribution of red blood cells throughout the column allows for easy grading of reaction strength. Gel technology is used for blood grouping, antibody screening and identification, compatibility testing, and other immunohaematology applications. It provides standardized, efficient and reliable results compared to conventional methods.
Blood can be separated into components like red blood cells, platelets, cryoprecipitate, and frozen plasma which are useful for different medical purposes. Whole blood is rarely used now due to the risk of volume overload. The Coombs test, also known as the antiglobulin test, detects the presence of antibodies and can be performed directly on a patient's red blood cells or indirectly by incubating their serum with donor red blood cells. A positive result in either test indicates the presence of antibodies.
This document discusses several major blood group systems including Lewis, I, P, MNSs, Kell, Kidd, Duffy, Lutheran, Bg, Sda, and Xg. It provides information on the antigens and genes involved in each system, the clinical significance of associated antibodies, and inheritance patterns. Some key points covered include that Lewis, I, and P antigens produce cold-reacting antibodies while Kell, Kidd, and Duffy produce warm-reacting antibodies. The MNSs, Kell, and Kidd systems can produce clinically significant antibodies implicated in hemolytic transfusion reactions and hemolytic disease of the newborn.
The document provides an overview of crossmatching procedures in blood banking. It discusses the types of crossmatches, including major and minor crossmatches. It describes the steps involved in immediate spin, tube method, and gel card crossmatches. It also covers causes and approaches to dealing with incompatible crossmatches. The roles of antibody screening, direct antiglobulin test, and autocontrol are explained. The document discusses common alloantibodies and autoantibodies. It provides details on Coombs testing and antibody titration.
the presentation will allow you to identify the different state maturation of RBC and to see the the different abnormally including the cell membrane abnormality , the inclusion bodies may appear in RBC ,and other cell abnormality.
1) The ABO blood group system was discovered in 1901 by Karl Landsteiner who identified the A, B, and O blood groups.
2) The blood groups are determined by the presence of antigens on red blood cells and the presence of antibodies in the plasma.
3) The ABO blood groups are inherited following Mendelian principles with A and B genes being co-dominant and O being recessive.
Kell blood group system most important blood group system following to ABO and Rh blood group system, particularly RhD as far as immunogenicity is concerned and Its clinical importance.
description about RBC membrane and its structural peculiarities,how it differs from other cells of our body. How this specialized cell manage homeostasis and function in a well defined manner. This presentation will also help in understanding various RBC storage lesions ,an important aspect of blood banking.
This document summarizes ABO and Rh(D) blood grouping systems. It discusses the key points of:
- The ABO system including the antigens, antibodies produced, and inheritance patterns. Group O is the universal donor.
- The Rh system focuses on the D antigen. About 85% of people are Rh positive. Sensitization can be prevented with anti-D immunoglobulin.
- Testing methods for ABO and Rh(D) typing including cell typing with monoclonal antibodies and serum typing. Weak D phenotypes require additional testing to determine Rh status.
- Clinical significance of blood group matching for transfusions to prevent hemolytic transfusion reactions. Group AB is the universal recipient.
The document discusses ABO blood grouping methods and procedures. The two main methods are the slide method and spin tube method. The slide method uses glass slides while the spin tube method uses test tubes. Procedures include preparing red blood cell suspensions, adding blood and antisera to slides or tubes, incubating, and observing for agglutination. Quality control and potential sources of error are also outlined.
- Karl Landsteiner discovered the ABO blood group system in 1901 through experiments mixing red blood cells and serum between colleagues.
- The Rh blood group system was discovered in 1937 by Landsteiner and Alexander Wiener who believed it involved a similar antigen found in rhesus monkeys.
- Landsteiner's rule states that individuals develop antibodies to ABO blood group antigens that they do not possess, which is critical to understanding blood group compatibility.
1. An absolute eosinophil count (AEC) is a blood test that measures the number of eosinophils, a type of white blood cell, to help diagnose conditions involving allergic reactions, infections, and other medical issues.
2. The AEC test involves diluting a blood sample with a special solution to stain and count eosinophils under a microscope.
3. An increased eosinophil count may indicate allergies, parasites, or certain types of leukemia, while a decreased count can be associated with hyperadrenalism or Cushing's syndrome.
This document provides a summary of Grifols' line of immunohematology products, including reagents, instrumentation, and hemovigilance tools. The reagents section describes Grifols' DG Gel system, an 8-column gel card for blood typing and antibody screening/identification. Key features include Clear Card Technology for consistent clarity, and monoclonal antibodies for antigens in the ABO, Rh, and other blood groups. The system includes cards for general typing, confirmation, and other specialized applications. Instrumentation includes both automated and manual/semiautomated devices. The hemovigilance section describes software tools for monitoring transfusion safety.
The document discusses blood group antigens, antibodies, and complement. It explains that:
1) Individuals normally produce antibodies against the A and/or B antigens absent from their red blood cells, which produce strong reactions during ABO testing.
2) ABO antibodies are initiated at birth but the titer is too low until 3-6 months of age, so tests before then are invalid due to maternal antibodies. Antibody production peaks between 5-10 years then declines in later life.
3) The A, B, and H antigens are constructed on oligosaccharide chains from a precursor substance and are fully developed after age 2-4, remaining constant throughout life. The H antigen is the precursor
This document discusses blood group systems, specifically ABO and Rh blood groups. It provides details on:
- The antigens found on red blood cell membranes that determine blood type
- Landsteiner's discovery of the ABO blood group system in 1900 and the four main blood types (A, B, AB, and O)
- The antigens and antibodies present in each blood type
- Rh blood group system including the Rho(D) antigen and typing only for Rho(D) to determine Rh status
- Techniques for blood typing including tube, slide, microplate, and newer gel/cassette methods
- Interpreting and resolving discrepancies in blood typing results
This document provides an overview of basic principles of immunohematology. It defines key terms like antigen and antibody. It describes the characteristics of antigens and factors that contribute to antigen immunogenicity. It also discusses the different types of immunoglobulins involved in blood group antibodies, and the differences between naturally occurring versus immune antibodies. Finally, it explains the stages of antigen-antibody reactions including sensitization and agglutination, and factors that can influence these reactions.
The document discusses the ABO blood group system. Some key points:
- Karl Landsteiner discovered the ABO blood group system in 1900-1901. It identifies four main blood groups: A, B, AB, and O.
- The presence or absence of A and B antigens on red blood cells determines an individual's blood group. Those without A or B antigens are group O.
- Anti-A and anti-B antibodies are naturally present in people's blood, developing after exposure to environmental antigens. These antibodies can cause hemolytic transfusion reactions if incompatible blood is transfused.
- The ABO blood groups are determined genetically based on inheritance of A, B, or O alleles. The A
A blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs). These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system.
This document discusses various techniques used in blood banking and transfusion medicine, including:
1. Pretransfusion testing involves ABO/Rh typing, antibody screening, and crossmatching to select compatible blood and prevent hemolytic transfusion reactions.
2. Antibody identification uses a panel of red blood cells to identify the specific antibody in a patient's serum through various testing phases including immediate spin, LISS incubation, and antiglobulin.
3. Special techniques like elution, hemagglutination inhibition, and titration are used to further characterize antibodies or quantify their concentration.
The document summarizes key information about the rare Bombay blood group:
- The Bombay blood group lacks the H, A, and B antigens found on red blood cells, and those with this group have antibodies against these antigens in their plasma. It was first discovered in Bombay, India and is more common in some parts of India.
- Those with the Bombay blood group must receive blood from other Bombay donors, as their red blood cells will hemolyze if transfused with blood containing the H, A, or B antigens. Testing for this rare group involves reverse grouping or detection of H antibodies.
- It is important for those with the Bombay blood group to register with blood banks
This document provides an overview of antibody identification in blood banking. It discusses the key steps in performing an antibody panel, including using a panel of known red blood cells to test against a patient's unknown serum. The goal is to identify any unexpected antibodies in the patient's serum. It also covers interpreting panel results, such as ruling out non-reactive antigens and looking for a matching antigen pattern. Techniques for identifying multiple antibodies like selected cells, neutralization, and chemical treatments are also outlined.
This document discusses thalassemias and hemoglobinopathies. It begins by outlining the objectives and topics to be covered, which include pathophysiology, classification, laboratory testing correlations, and treatment. The main types and classifications of alpha and beta thalassemias are then defined. Characteristics, demographics, genetics, terminology and classifications of hemoglobinopathies are also introduced. Details on primary and secondary laboratory investigations and correlations are provided. The document closes with descriptions of treatment approaches and management considerations for thalassemias and hemoglobinopathies.
Gel technology provides an innovative approach to performing various tests in immunohaematology with improved sensitivity and specificity compared to conventional tube techniques. It involves centrifuging red blood cells through a gel column where agglutination reactions occur. The distribution of red blood cells throughout the column allows for easy grading of reaction strength. Gel technology is used for blood grouping, antibody screening and identification, compatibility testing, and other immunohaematology applications. It provides standardized, efficient and reliable results compared to conventional methods.
Blood can be separated into components like red blood cells, platelets, cryoprecipitate, and frozen plasma which are useful for different medical purposes. Whole blood is rarely used now due to the risk of volume overload. The Coombs test, also known as the antiglobulin test, detects the presence of antibodies and can be performed directly on a patient's red blood cells or indirectly by incubating their serum with donor red blood cells. A positive result in either test indicates the presence of antibodies.
This document discusses several major blood group systems including Lewis, I, P, MNSs, Kell, Kidd, Duffy, Lutheran, Bg, Sda, and Xg. It provides information on the antigens and genes involved in each system, the clinical significance of associated antibodies, and inheritance patterns. Some key points covered include that Lewis, I, and P antigens produce cold-reacting antibodies while Kell, Kidd, and Duffy produce warm-reacting antibodies. The MNSs, Kell, and Kidd systems can produce clinically significant antibodies implicated in hemolytic transfusion reactions and hemolytic disease of the newborn.
The document provides an overview of crossmatching procedures in blood banking. It discusses the types of crossmatches, including major and minor crossmatches. It describes the steps involved in immediate spin, tube method, and gel card crossmatches. It also covers causes and approaches to dealing with incompatible crossmatches. The roles of antibody screening, direct antiglobulin test, and autocontrol are explained. The document discusses common alloantibodies and autoantibodies. It provides details on Coombs testing and antibody titration.
the presentation will allow you to identify the different state maturation of RBC and to see the the different abnormally including the cell membrane abnormality , the inclusion bodies may appear in RBC ,and other cell abnormality.
1) The ABO blood group system was discovered in 1901 by Karl Landsteiner who identified the A, B, and O blood groups.
2) The blood groups are determined by the presence of antigens on red blood cells and the presence of antibodies in the plasma.
3) The ABO blood groups are inherited following Mendelian principles with A and B genes being co-dominant and O being recessive.
Kell blood group system most important blood group system following to ABO and Rh blood group system, particularly RhD as far as immunogenicity is concerned and Its clinical importance.
description about RBC membrane and its structural peculiarities,how it differs from other cells of our body. How this specialized cell manage homeostasis and function in a well defined manner. This presentation will also help in understanding various RBC storage lesions ,an important aspect of blood banking.
This document summarizes ABO and Rh(D) blood grouping systems. It discusses the key points of:
- The ABO system including the antigens, antibodies produced, and inheritance patterns. Group O is the universal donor.
- The Rh system focuses on the D antigen. About 85% of people are Rh positive. Sensitization can be prevented with anti-D immunoglobulin.
- Testing methods for ABO and Rh(D) typing including cell typing with monoclonal antibodies and serum typing. Weak D phenotypes require additional testing to determine Rh status.
- Clinical significance of blood group matching for transfusions to prevent hemolytic transfusion reactions. Group AB is the universal recipient.
The document discusses ABO blood grouping methods and procedures. The two main methods are the slide method and spin tube method. The slide method uses glass slides while the spin tube method uses test tubes. Procedures include preparing red blood cell suspensions, adding blood and antisera to slides or tubes, incubating, and observing for agglutination. Quality control and potential sources of error are also outlined.
- Karl Landsteiner discovered the ABO blood group system in 1901 through experiments mixing red blood cells and serum between colleagues.
- The Rh blood group system was discovered in 1937 by Landsteiner and Alexander Wiener who believed it involved a similar antigen found in rhesus monkeys.
- Landsteiner's rule states that individuals develop antibodies to ABO blood group antigens that they do not possess, which is critical to understanding blood group compatibility.
1. An absolute eosinophil count (AEC) is a blood test that measures the number of eosinophils, a type of white blood cell, to help diagnose conditions involving allergic reactions, infections, and other medical issues.
2. The AEC test involves diluting a blood sample with a special solution to stain and count eosinophils under a microscope.
3. An increased eosinophil count may indicate allergies, parasites, or certain types of leukemia, while a decreased count can be associated with hyperadrenalism or Cushing's syndrome.
This document provides a summary of Grifols' line of immunohematology products, including reagents, instrumentation, and hemovigilance tools. The reagents section describes Grifols' DG Gel system, an 8-column gel card for blood typing and antibody screening/identification. Key features include Clear Card Technology for consistent clarity, and monoclonal antibodies for antigens in the ABO, Rh, and other blood groups. The system includes cards for general typing, confirmation, and other specialized applications. Instrumentation includes both automated and manual/semiautomated devices. The hemovigilance section describes software tools for monitoring transfusion safety.
The document discusses blood group antigens, antibodies, and complement. It explains that:
1) Individuals normally produce antibodies against the A and/or B antigens absent from their red blood cells, which produce strong reactions during ABO testing.
2) ABO antibodies are initiated at birth but the titer is too low until 3-6 months of age, so tests before then are invalid due to maternal antibodies. Antibody production peaks between 5-10 years then declines in later life.
3) The A, B, and H antigens are constructed on oligosaccharide chains from a precursor substance and are fully developed after age 2-4, remaining constant throughout life. The H antigen is the precursor
The Rh blood group system is one of the most complex with over 50 antigens. The Rh factor refers to the presence (Rh positive) or absence (Rh negative) of the D antigen on red blood cells. The RHD gene encodes the D antigen, while the RHCE gene encodes the C, c, E, and e antigens. Rh proteins are integral membrane proteins that may function in ammonium or carbon dioxide transport. The Rh system was discovered in 1939 when a woman had an immune response after transfusion with her husband's Rh positive blood after delivering a stillborn infant. This led to the identification of the highly immunogenic Rh antigens which can cause hemolytic transfusion reactions or hemolytic disease of the newborn if a
The document discusses the field of immunohematology, which involves the study of antigen-antibody reactions related to blood components. It covers topics like blood group systems, common tests used in immunohematology like blood typing and crossmatching, hemolytic disease of the newborn, and complications of blood transfusion.
This document discusses inflammation and its triggers, objectives, and results. It describes the cardinal signs of inflammation as redness, swelling, heat, pain, and loss of function. It differentiates between acute and chronic inflammation and discusses the cells, mediators, and systemic effects involved in each. Key aspects of the inflammatory response like increased vascular permeability, leukocyte recruitment, recognition of pathogens, and termination signals are explained.
The document discusses the Rhesus blood group system, which was discovered in 1940 after testing human blood with rabbit antiserum against rhesus monkey red blood cells. It is one of the most polymorphic blood group systems, with over 45 antigens. The Rh system involves two closely linked genes, RHD and RHCE, which determine the presence of various antigens like D, C, c, E, and e. An Rh incompatibility during pregnancy can cause hemolytic disease of the newborn if a mother is Rh negative and carries an Rh positive baby.
This document discusses Rh (Rhesus) isoimmunization, which occurs when an Rh-negative pregnant mother develops antibodies against Rh-positive fetal blood cells. The key points are:
- Anti-D antibody is the most common cause, though anti-Kell, anti-c and anti-E can also cause hemolytic disease of the newborn.
- MCA Doppler of the fetal brain and amniocentesis to measure bilirubin levels (delta OD450) can assess the severity of fetal anemia.
- Prevention involves administering Rhogam prophylaxis to sensitized mothers during and after pregnancy to prevent antibody development.
- Clinical management may include monitoring antibody tit
The document discusses Rh factor incompatibility, which occurs when a pregnant woman who is Rh negative carries a baby who is Rh positive. This can cause the woman's immune system to produce antibodies against the baby's Rh positive blood. For future pregnancies, these antibodies can cross the placenta and destroy the baby's red blood cells, potentially causing mild to fatal anemia. Rh incompatibility is detected via blood tests and treated with medications during pregnancy like RhoGAM to prevent antibody production or exchange transfusions for the baby if issues occur. Proper Rh typing and compatibility testing prevents dangerous transfusion reactions.
1) The document discusses the ABO and Rh blood group systems, which are determined by antigens on red blood cells and the corresponding antibodies found in plasma.
2) The ABO system includes A, B, and H antigens that are synthesized based on inherited genes. The presence or absence of A and B antigens determines the four blood groups - A, B, AB, and O.
3) The Rh system involves the RhD antigen. Most individuals are RhD-positive unless they inherit two copies of the RhD-negative gene.
The ABO blood group system is one of the most important blood group systems for transfusions. It is determined by the ABO gene which has three alleles - IA, IB, and i - that encode for the A, B, and O antigens, respectively. The ABO blood types are determined by which alleles are present: IAIA or IAi produce type A blood, IBIB or IBi produce type B, IAIB produces AB, and ii produces O. The Rh system, specifically the D antigen, is the second most important blood group and sensitization to the D antigen in Rh-negative pregnant women can cause hemolytic disease of the newborn if not prevented.
The document summarizes the history and science behind blood grouping and the ABO blood group system. It describes how Karl Landsteiner discovered the major ABO blood groups in 1901. It explains the antigens and antibodies present in each blood group according to Landsteiner's rule. The genetics and biochemistry of the ABO blood group system are covered, including how the H, A, and B antigens are synthesized on red blood cells. Common blood grouping techniques like forward and reverse grouping are also summarized.
The document discusses blood grouping methodology and focuses on the ABO and Rh blood group systems. It provides details on:
- The ABO blood group system is the most clinically important and is determined by the presence or absence of A and B antigens on red blood cells. The Rh system involves the D antigen.
- Karl Landsteiner discovered the ABO system in 1901 and was awarded the Nobel Prize for his work. Inheritance of blood groups follows genetic patterns.
- Common techniques for blood grouping include slide tests, tube tests, microplates, and gel cards. Proper sample collection and testing procedures must be followed.
- Understanding blood groups is essential for safe blood transfusions and preventing issues like hemolytic
This document provides information about ABO and Rh blood grouping and typing. It discusses the antigens and antibodies involved in these two major blood group systems, including their inheritance, development, expression, and clinical significance. The key points covered include the reciprocal relationship between ABO antigens and antibodies, development of ABO antigens at birth, subgroups within the ABO system, inheritance of ABO blood groups, anti-A and anti-B antibody production, and Rh (D) antigen properties and typing. Practical aspects of ABO and Rh blood grouping using tube, gel, and microplate methods are also described.
This presentation summarizes the ABO blood group system. It defines blood groups as classifications based on antigens on red blood cells, with over 30 known human blood group systems containing over 600 antigens. The ABO system was discovered by Karl Landsteiner in 1901 and recognizes the A, B, AB, and O blood groups based on the presence or absence of A and B antigens. The characteristics of each blood group determine who can donate to and receive from whom. ABO antibodies are clinically significant as they are naturally occurring, universal, and highly reactive. Understanding blood groups is important for transfusion safety and other medical applications.
12- Blood Groups and Blood Transfusion 2018-converted.pptxgimspathcme2022
Blood typing involves determining the presence of antigens on red blood cells. The ABO and Rh blood group systems are most important for blood transfusions. Karl Landsteiner discovered the ABO blood groups in 1901 and was awarded the Nobel Prize for this work. The ABO blood groups are determined by the presence of A and B antigens, and antibodies against antigens not present. Type O blood lacks both antigens and can be donated to all groups, while Type AB has both antigens and is a universal recipient. Compatible blood typing and cross-matching between donor and recipient prevents transfusion reactions.
The document discusses ABO and Rh blood group systems. It provides details on the antigens and antibodies involved, including their reciprocal relationship. It explains how the ABO blood groups are inherited based on codominance and gives examples of inheritance patterns. The synthesis of ABO antigens is described, showing how genes code for enzymes that add specific sugars to produce the A, B, or H antigens. Practical aspects of blood grouping techniques like the tube method and microplate method are also covered.
The ABO blood group system is the most important for transfusion medicine. It includes four main groups - A, B, O, and AB - determined by the presence or absence of antigens on red blood cells. ABO incompatibility between donor and recipient blood can cause hemolytic transfusion reactions. Resolving discrepancies in ABO typing results is important and may be due to technical errors, subgroups, or medical conditions affecting antibody production.
Karl Landsteiner discovered the main human blood groups (A, B, AB, and O) in 1901. This discovery allowed safer blood transfusions by preventing fatal reactions between incompatible blood groups. The presence or absence of antigens (proteins) and antibodies on red blood cells and in plasma determines a person's blood group. The ABO and Rh blood group systems are most important for transfusions. A person can only receive blood from a donor with compatible or identical blood groups to prevent dangerous clumping of red blood cells.
There are over 30 known blood group systems that contain around 400 antigens. The most important systems for blood transfusions are ABO and Rh. The ABO system contains A, B, and H antigens. People are type A, B, AB, or O based on which antigens are present. Naturally occurring antibodies are directed against antigens not present on one's own red blood cells. The Rh system contains D and other antigens. Most people are Rh+ or Rh-. Rh incompatibility can cause hemolytic disease of the newborn.
The document summarizes information about blood group systems and the Kidd blood group system. It discusses that there are over 30 recognized blood group systems in addition to ABO and Rh, including the Kidd system. The Kidd system involves the Jka and Jkb antigens which are carried on the SLC14A1 glycoprotein and can cause hemolytic transfusion reactions. It notes that a patient developed anti-Jkb antibodies after transfusions and now requires Jkb negative blood, which can be difficult to find due to availability and donor frequencies of the antigen.
This document summarizes blood grouping and cross-matching techniques. It discusses that blood grouping is based on antigens on red blood cells, with ABO and Rh being most clinically important. It then describes Landsteiner's discovery of blood groups, antigens, antibodies, and his law. Methods for blood grouping like slide and tube methods are outlined. The document also discusses Rh blood group system, antigens, antibodies, importance in transfusion and disease. It concludes with describing cross-matching techniques and their role in safe blood transfusions.
The document discusses the ABO blood group system, including its discovery, genetics, biochemistry, antigens, antibodies, and implications for transfusion. Some key points:
- Karl Landsteiner discovered the main ABO blood groups (A, B, AB, O) in 1900. The ABO blood type is determined by alleles at a single gene locus.
- The antigens are carbohydrate structures on red blood cells. People naturally produce antibodies against antigens they lack.
- ABO typing must be accurate to avoid transfusion reactions. Discrepancies can occur due to weak subgroups, diseases, or test issues. Resolving discrepancies helps ensure patient and donor safety.
The document summarizes key information about blood groups and genetics. It discusses how blood type is determined by genes inherited from parents, which cause proteins called agglutinogens on red blood cells. The main blood group systems - ABO and Rh - are described. Landsteiner's laws explain the relationship between agglutinogens and agglutinins in different blood types. Compatibility between donor and recipient blood depends on preventing agglutination during transfusion. Complications can be avoided through proper cross-matching and screening of donor blood.
Blood groups are determined by the presence or absence of antigens on red blood cells. The main blood group systems are ABO and Rh. The ABO system categorizes blood into 4 main groups based on A and B antigens: A, B, AB, and O. The Rh system categorizes blood as Rh positive or Rh negative based on the presence of the D antigen. Incompatibility between donor and recipient blood antigens can cause agglutination, where red blood cells clump together. Understanding blood groups is important for safe blood transfusions.
This document discusses blood grouping and Rh typing. It begins by explaining that blood grouping is based on the antigens present on red blood cells, with ABO and Rh being clinically most important. It then covers the history and discovery of blood grouping, the various blood group systems, and Landsteiner's law regarding antigens and antibodies. The rest of the document discusses methods for blood grouping and Rh typing, including slide and tube methods. It covers interpretation of results, universal donor/recipient groups, and rare Bombay blood group. The importance and techniques of cross-matching for blood transfusions are also summarized.
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3.inmuno hematología.inmunologia.2011.dr hilario
1. Immunohematology
Dr. Julio Hilario Vargas
Department of Physiology
School of Medicine
National University of Trujillo
2. DEFINICION
Es la parte de la hematología que estudia los
procesos inmunitarios que tienen lugar en el
organismo en relación con los elementos
sanguíneos.
Uno de los aspectos más importantes de la
inmunohematología es el estudio y cuantificación
de los grupos sanguíneos eritrocitarios que son
componentes antigénicos presentes en la
superficie de los hematíes, ya que se relaciona
directamente con la terapéutica transfusional y la
prevención de accidentes hemolíticos graves.
3. En primer termino se identificaron sobre los hematíes,
pero luego se describieron determinantes antigénicos
plaquetarios, leucocitarios y séricos.
Los genes determinantes de los grupos sanguíneos
transmiten, generalmente, caracteres codominantes ( se
expresan en homocigotos y heterocigotos).
Existen genes “amorfos” que no generan productos que
puedan ser identificados como antígenos ( ej: gen d)
Todos los antígenos de los grupos sanguíneos han sido
definidos serológicamente por la presencia de sus
anticuerpos correspondientes.
4. Immunohematology
Merges aspects of hematology, immunology &
genetics
Serologic, genetic, biochemical and molecular
study of antigens associated with membrane
structures on the cellular constituents of the blood
Immunologic reactions involving all blood
components and constituents
Primary immunological components: antigens &
antibodies provides basis for blood bank testing
and reactions
5. CARDINAL RULE IN BLOOD BANK:
Antigens are found on the surface of
red blood cells and the antibodies
are found in serum or plasma
7. ANTIGENS
Substances that have the capability to stimulate the
production of an antibody
Characteristics:
1. Chemical nature – protein, CHO, nucleic acid or
lipopolysaccharide
2. Molecular weight > 10,000 daltons
3. Complexity – more complex, > antibody
stimulation
4. Stability – if unstable degrade less Ab
stimulation
5. Foreign
8. Chemical composition of antigens
1. Glycoproteins & lipoproteins – most potent
Glycolipids
2. Pure polysaccharides – not immunogenic except in
humans and mice
3. Pure lipids & nucleic acids – not immunogenic but
can be antigenic serve as haptens
9. Grupos Eritrocitarios y sus Antígenos
ANTÍGENOS MAS
GRUPOS SANGUÍNEOS
IMPORTANTES
ABO A, B, AB, O
Rh D, C, c, E, e
MNS M, N, S, s, U
Lewis Lea, Leb
P P1, P2
Lutheran Lua, Lub
Kell K, k, Kpa, Kpb
Duffy Fya, Fyb
Kidd Jka, Jkb
10. SISTEMAS DE GRUPOS SANGUINEOS
Grispan S. Rev. Medica Hondur. 51:103-14. 1983
11. Immunogenicity of Blood Group Antigens
A, B and D (Rho) – most immunogenic
Kell (K)
Duffy: Fya
Fyb
Kidd:Jka
Jkb
12. ANTIBODIES
Also called immunoglobulins
Characteristics:
1. Protein
2. Produced in response to stimulation by an antigen
3. Specific for the stimulating antigen
- Consists of 2 heavy chains & 2 light chains held
together by disulfide bonds
- Produce 3 fragments when cleaved by enzymes 2
Ag- binding fragments (Fab) & 1 crystallizable fragment
(Fc)
13. Classification of Blood Group Antibodies
Alloantibodies
Reacts with foreign Ag not present on patient’s own
RBC
Most produced as result of immune stimulation via
transfusion or pregnancy (usually during
delivery)
Autoantibodies
Reacts with an Ag on patient’s own cells & with that
same Ag on the cells of other individuals
14. ABO BLOOD GROUP SYSTEM
Discovered by Karl Landsteiner; locus on chr 9
Single most important blood group for the selection
and transfusion of blood
Widely expressed tissues & body fluids including
red cells, platelets & endothelial cells
Three antigens: A, B, H
Two major antibodies: anti-A and anti-B
Four phenotypes: A, B, AB, O A & B Ag’s
autosomal co-dominant (expressed on grp A, B and
AB red cells; O phenotype autosomal recessive
(most frequent)
16. ABO BLOOD GROUP SYSTEM
ABO Antigens
Present on the surface of red cells as well as tissue
and endothelial cells in the body
Found in soluble form in plasma & other body
secretions in people known as secretors
Inherited in simple Mendelian fashion from an
individual’s parents
3 possible genes that can be inherited: A, B, O
A and B genes produce a detectable product
O gene does not produce a detectable product
17. ABO BLOOD GROUP SYSTEM
ABO System
Phenotype Antigen Natural Genotype
antibody
A A only Anti-B AA or AO
B B only Anti-A BB or BO
AB A and B None AB
Anti-A,
O None OO
Anti-B
18. ABO BLOOD GROUP SYSTEM
A and B genes do not directly produce
antigens enzymatic reaction products of
enzymes called glycosyltransferases
attaches a sugar molecule to the chemical
structure of the antigen sugar molecule
responsible for specificity
O antigen no transferase no antigen
produced
A and B antigens on surface of RBC
protrude from outermost layer of cell
membrane
21. ABO BLOOD GROUP SYSTEM
Antigen formation
H antigen B antigen B antigen
22. ABO Blood Group System
http://www.ncbi.nlm.nih.gov/gv/rbc/xslcgi.fcgi?cmd=bgmut/systems_info&system=abo
23. ABO BLOOD GROUP SYSTEM
H Antigen
Required to produce either A or B antigens
possible genetic combinations: HH, Hh, or hh
HH or Hh (+) produce H Ag 99.99% of
Caucasians
hh does not produce H Ag Bombay
phenotype (Oh)
Anti-H antibodies rare – found only in individuals
with Bombay phenotype
24. ABO BLOOD GROUP SYSTEM
Example of determining offspring blood types
from known or suspected genotypes:
Genotype parent #1 (AO)
A O
Genotype parent A AA AO
#2 (AB) B AB BO
Phenotypes of possible offsprings: A, AB, B
25. ABO BLOOD GROUP SYSTEM
Frequencies of ABO Blood Groups:
Blood Group Frequency
O 45%
A 41%
B 10%
AB 4%
26. ABO BLOOD GROUP SYSTEM
ABO Subtypes:
1. A variants (A1, A2)
A1 most common (80%) & most antigenic
A1 and A2 differentiated using antisera specific
for A1 Ag (anti-A1 lectin) prepared from seed
known as Dolichos biflorus (+) reaction
with A1 but not A2
Anti-A reacts with both A1 & A2 but more
strongly with A2
27. ABO BLOOD GROUP SYSTEM
ABO Subtypes:
2. Weak A and weak B phenotypes
3. Null phenotypes:
(a) Bombay (Oh)
No A, B or H Ag on red cells & secretions
With anti-A, anti-B & anti-H in their sera
(b) para-Bombay
Absent or only trace A,B & H Ag’s detected
on rbc w/ normal expression in
secretions & body fluids
28. ABO BLOOD GROUP SYSTEM
ABO Antibodies
Natural antibodies antigenic stimulus is
environmental exposure occurs from birth
Newborns without ABO antibodies of their own;
begin to produce Ab with detectable titer at 6
months of age
Other characteristics of ABO antibodies:
- IgM
- Reacts at room temp. after an immediate spin
29. ABO ROUTINE TESTING
(slide or test tube method)
DIRECT TYPING
- test for antigens
- patient’s cells containing unknown antigens tested with
known antisera
- antisera manufactured from human sera
- antisera used:
Antisera Color Source
Anti-A Blue Group B donor
Anti-B Yellow Group A donor
Anti-A,B Clear Group O donor
30. ABO ROUTINE TESTING
Reaction Patterns for ABO Groups
Agglutination Agglutination
Blood group
with Anti-A with Anti-B
A + -
B - +
AB + +
O - -
31. ABO ROUTINE TESTING
INDIRECT/REVERSE TYPING
- Known antigen (cell) vs. unknown antibody
(patient’s serum)
- Serum is combined with cells having known Ag
content in a 2:1 ratio
- Uses commercially prepared reagents
containing saline-suspended A1 and B cells
32. ABO ROUTINE TESTING
Reaction Patterns for ABO Groups
Agglutination Agglutination
Blood Group
with A cells with B cells
A - +
B + -
AB - -
O + +
33. ABO ROUTINE TESTING
Causes of Discrepancies in ABO Testing
Technical
1. Incorrect ID/recording
2. Patient/donor serum not added
3. Reagent contamination
4. Under-/over-centrifugation
5. Hemolysis
6. Warming of test mixture
34. ABO ROUTINE TESTING
Causes of Discrepancies in ABO Testing
B. Red Blood Cells
1. Missing or weak A/B antigen
2. Acquired B Ag – colon or gastric CA,
intestinal obstruction
3. Polyagglutinable RBC
4. Ab-coated RBC – post-transfusion incompatibility;
autoimmune hemolytic anemia
5. Maternal-fetal agglutination – mismatched
transfusion
35. ABO ROUTINE TESTING
Causes of Discrepancies in ABO Testing
C. Serum
1. Roleaux formation – presence of
plasma expanders, monoclonal
gamma globulins
2. Anti-A1
3. Unexpected alloantibodies
4. Expected antibody absent –
Roleaux
hypogammaglobulinemia, extreme
ages, immunosuppression
36. Rh BLOOD GROUP SYSTEM
- Discovered in 1940 by Landsteiner &
Wiener
- Most complex erythrocyte antigen
system; located on chromosome 1
- Found exclusively on surface of rbc
integral part of red cell membrane
- Primary antigen if present, consider
Rh (+)
- Lack corresponding naturally-occurring
antibodies in serum
38. Rh BLOOD GROUP SYSTEM
CLASSIFICATION/NOMENCLATURE SYSTEM
Wiener
Multiple allele hypothesis
5 antigens: Rho, rh’, rh”, hr’, hr”
Single locus inheritance system with 8 alternate
common alleles coding for agglutinogens
1 individual produces 2 agglutinogens
inherited from both parents
39. Rh BLOOD GROUP SYSTEM
CLASSIFICATION/NOMENCLATURE SYSTEM
Fischer & Race
Three alleles: D/d, C/c and E/e
Five antigens: D, C, E, c, e
d no D locus no antigenic products
Rosenfeld
Numerical system
Rh1 to Rh5
40. Comparación de las nomenclaturas para
los Ag del Sistema Rh
Wiener Fisher –Race Rosenfield
Rho D Rh1
rh` C Rh2
rh” E Rh3
h`r c Rh4
hr” e Rh5
41. Rh BLOOD GROUP SYSTEM
Presence of D = presence of Rho factor
Rh (+)
Absence of D Rh (-)
42. Rh BLOOD GROUP SYSTEM
Testing for Rho (D) Antigen
- Use antisera originating from human source
- Antisera with different constituents use of
high protein media necessary to produce
agglutination since antigens are an integral part of
the red cell membrane less numerous than ABO
antigens
43. Rh BLOOD GROUP SYSTEM
Testing for Du Variant
Use bovine or albumin-suspended anti-D
reagent
Incubate at 37oC for 15-60 minutes to facilitate
formation of Ag-Ab complex
Interpretation: (+) Du consider Rh (+)
Person who appear to be Rh (-) should be
proven to be Du (-) before they are considered
to be eligible to receive transfusion
44. Rh BLOOD GROUP SYSTEM
Rh Antibodies
- Not naturally-occurring immune antibodies
produced upon sensitization IgG isotype
- Reactive at 37oC enhanced with enzyme-
treated red cells
- Can cross the placenta
- Associated with hemolytic transfusion
reaction and hemolytic disease of the newborn
(HDN)
45. Rh BLOOD GROUP SYSTEM
Rh Typing – slide or test tube method
False (+) results
a. Drying
b. Roleaux formation
c. Auto-agglutination
d. Patient’s red cells heavily coated with Ab’s
e. Presence of cold agglutinins
46. Rh BLOOD GROUP SYSTEM
Rh Typing
False (-) results
a. Use of old cells
b. Wrong cell concentration
c. Hemolysis
d. Inadequate mixing of cells
e. Inactive typing sera
f. Incorrect temperature
g. Existence of Du variant
h. High concentration of blocking antibodies
47. HEMOLYTIC DISEASE OF THE NEWBORN
Involves hemolysis of red cells in the fetus and
neonate
Antibody is present in the mother that
corresponds to an antigen on the surface of
the red cells of the fetus Ab crosses
placenta attaches to fetal Ag hemolyze
red cells of fetus
Differential diagnosis: physiologic jaundice,
septicemia, CID, toxoplasmosis, congenital
syphilis
48. HEMOLYTIC DISEASE OF THE NEWBORN
Comparison of ABO versus Rh HDN
Characteristic ABO HDN
First pregnancy Yes Rare
Disease predicted by titers No Yes
Antibody IgG Yes (anti-A,B) Yes (anti-D)
Bilirubin at birth Normal range Elevated
Anemia at birth No Yes
Phototherapy Yes Yes
Exchange transfusion Rare Common
Intrauterine transfusion None Sometimes
Spherocytosis Yes Rare