POLARIZATION
Polarization is a property of waves that can oscillate with more than one orientation.
Electromagnetic waves such as light exhibit polarization, as do some other types of wave, such as gravitational waves.
Sound waves in a gas or liquid do not exhibit polarization, since the oscillation is always in the direction the wave travels.
Interference of light refers to the redistribution of light energy due to superposition of two light waves. This superposition leads to a pattern of alternate dark and bright fringes. These dark and bright fringes are called as minima and maxima respectively.
Polarization of Light and its Application (healthkura.com)Bikash Sapkota
Download link ❤❤https://healthkura.com/eye-ppt/29/❤❤
Dear viewers Check Out my other piece of works at ❤❤❤ https://healthkura.com/eye-ppt/ ❤❤❤
polarization of light & its application.
PRESENTATION LAYOUT
Concept of Polarization
Types of Polarization
Methods of achieving Polarization
Applications of Polarization
POLARIZATION
Transforming unpolarized light into polarized light
Restriction of electric field vector E in a particular plane so that vibration occurs in a single plane
Characteristic of transverse wave
Longitudinal waves can’t be polarized; direction of their oscillation is along the direction of propagation.............
For Further Reading
•Optics by Tunnacliffe
•Optics and Refraction by A.K. Khurana
•Principle of Physics, Ayam Publication
•Internet
POLARIZATION
Polarization is a property of waves that can oscillate with more than one orientation.
Electromagnetic waves such as light exhibit polarization, as do some other types of wave, such as gravitational waves.
Sound waves in a gas or liquid do not exhibit polarization, since the oscillation is always in the direction the wave travels.
Interference of light refers to the redistribution of light energy due to superposition of two light waves. This superposition leads to a pattern of alternate dark and bright fringes. These dark and bright fringes are called as minima and maxima respectively.
Polarization of Light and its Application (healthkura.com)Bikash Sapkota
Download link ❤❤https://healthkura.com/eye-ppt/29/❤❤
Dear viewers Check Out my other piece of works at ❤❤❤ https://healthkura.com/eye-ppt/ ❤❤❤
polarization of light & its application.
PRESENTATION LAYOUT
Concept of Polarization
Types of Polarization
Methods of achieving Polarization
Applications of Polarization
POLARIZATION
Transforming unpolarized light into polarized light
Restriction of electric field vector E in a particular plane so that vibration occurs in a single plane
Characteristic of transverse wave
Longitudinal waves can’t be polarized; direction of their oscillation is along the direction of propagation.............
For Further Reading
•Optics by Tunnacliffe
•Optics and Refraction by A.K. Khurana
•Principle of Physics, Ayam Publication
•Internet
This article discusses the basics of Interference phenomenon of light. Young's Double Slit Experiment is discussed to understand the phenomenon of Interference and also to understand the wave behaviour of light. Newton's Ring experiment, Lloyd's Mirror experiment, Fresnel's Biprism experiment are studued here to establish the wave nature of light. Also the bright and the dark fringes and there mathematical expressions are elaborated here in this article.
This LO presents conditions for constructive and destructive thin film interference. An example of thin film interference in butter fly wings with a worked solution is provided to assist in application of the concepts and demonstrate the real life applications of this topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
2. Wave Optics
The wave nature of light is needed
to explain various phenomena
Interference
Diffraction
Polarization
3. Interference
Light waves interfere with each other
much like mechanical waves do
All interference associated with light
waves arises when the electromagnetic
fields that constitute the individual
waves combine
4. Conditions for Interference
For sustained interference between two sources of light
to be observed, there are two conditions which must be
met
The sources must be coherent
They must maintain a constant phase with
respect to each other
The waves must have identical wavelengths
5. Producing Coherent Sources
Light from a monochromatic source is allowed to pass
through a narrow slit
The light from the single slit is allowed to fall on a
screen containing two narrow slits
The first slit is needed to insure the light comes from a
tiny region of the source which is coherent
Old method
6. Producing Coherent Sources,
cont
Currently, it is much more common to use a laser as a
coherent source
The laser produces an intense, coherent, monochromatic beam
over a width of several millimeters
The laser light can be used to illuminate multiple slits directly
7. Young’s Double Slit Experiment
Thomas Young first demonstrated interference
in light waves from two sources in 1801
Light is incident on a screen with a narrow slit,
So
The light waves emerging from this slit arrive
at a second screen that contains two narrow,
parallel slits, S1 and S2
8. Young’s Double Slit
Experiment, Diagram
The narrow slits, S1 and
S2 act as sources of
waves
The waves emerging
from the slits originate
from the same wave
front and therefore are
always in phase
9. Resulting Interference
Pattern
The light from the two slits form a visible pattern on a
screen
The pattern consists of a series of bright and dark
parallel bands called fringes
Constructive interference occurs where a bright fringe
appears
Destructive interference results in a dark fringe
10. Fringe Pattern
The fringe pattern
formed from a Young’s
Double Slit Experiment
would look like this
The bright areas
represent constructive
interference
The dark areas represent
destructive interference
11. Interference Patterns
Constructive interference
occurs at the center point
The two waves travel the
same distance
Therefore, they arrive
in phase
12. Interference Patterns, 2
The upper wave has to
travel farther than the lower
wave
The upper wave travels one
wavelength farther
Therefore, the waves
arrive in phase
A bright fringe occurs
13. Interference Patterns, 3
The upper wave travels
one-half of a wavelength
farther than the lower wave
The trough of the bottom
wave overlaps the crest of
the upper wave
This is destructive
interference
A dark fringe occurs
15. Interference Equations, 2
For a bright fringe, produced by constructive interference, the
path difference must be either zero or some integral multiple
of the wavelength
δ = d sin θbright = m λ
m = 0, ±1, ±2, …
m is called the order number
When m = 0, it is the zeroth order maximum
When m = ±1, it is called the first order maximum
16. Interference Equations, 3
The positions of the fringes can be
measured vertically from the zeroth
order maximum
y = L tan θ L sin θ
Assumptions
L>>d
d>>λ
Approximation
θ is small and therefore the
approximation tan θ sin θ can
be used
17. Interference Equations, 4
When destructive interference
occurs, a dark fringe is observed
This needs a path difference of an
odd half wavelength
δ = d sin θdark = (m + ½) λ
m = 0, ±1, ±2, …
18. Interference Equations,
final
For bright fringes
For dark fringes
0, 1, 2bright
L
y m m
d
1
0, 1, 2
2
dark
L
y m m
d
19. Uses for Young’s Double Slit
Experiment
Young’s Double Slit Experiment provides a method for
measuring wavelength of the light
This experiment gave the wave model of light a great
deal of credibility
It is inconceivable that particles of light could cancel
each other
20. Phase Changes Due To
Reflection
An electromagnetic wave
undergoes a phase change of
180° upon reflection from a
medium of higher index of
refraction than the one in which
it was traveling
Analogous to a reflected
pulse on a string
21. Phase Changes Due To
Reflection, cont
There is no phase change
when the wave is reflected
from a boundary leading to
a medium of lower index of
refraction
Analogous to a pulse in
a string reflecting from a
free support
22. Lloyd’s Mirror
An arrangement for
producing an interference
pattern with a single light
source
Wave reach point P either by
a direct path or by reflection
The reflected ray can be
treated as a ray from the
source S’ behind the mirror
23. Interference Pattern from
the Lloyd’s Mirror
An interference pattern is formed
The positions of the dark and
bright fringes are reversed relative
to pattern of two real sources
This is because there is a 180°
phase change produced by the
reflection
24. Interference in Thin Films
Interference effects are
commonly observed in thin films
Examples are soap bubbles and oil on
water
The interference is due to the
interaction of the waves
reflected from both surfaces of
the film
25. Interference in Thin Films,
2
Facts to remember
An electromagnetic wave traveling from a
medium of index of refraction n1 toward a
medium of index of refraction n2 undergoes
a 180° phase change on reflection when n2
> n1
There is no phase change in the reflected wave if
n2 < n1
The wavelength of light λn in a medium
with index of refraction n is λn = λ/n where
λ is the wavelength of light in vacuum
26. Interference in Thin Films,
3
Ray 1 undergoes a phase
change of 180° with respect
to the incident ray
Ray 2, which is reflected
from the lower surface,
undergoes no phase change
with respect to the incident
wave
27.
28. Interference in Thin Films,
4
Ray 2 also travels an additional distance
of 2t before the waves recombine
For constructive interference
2nt = (m + ½ ) λ m = 0, 1, 2 …
This takes into account both the difference in
optical path length for the two rays and the 180°
phase change
For destruction interference
2 n t = m λ m = 0, 1, 2 …
29. Interference in Thin Films,
5
Two factors influence interference
Possible phase reversals on reflection
Differences in travel distance
The conditions are valid if the medium
above the top surface is the same as
the medium below the bottom surface
If the thin film is between two different
media, one of lower index than the film
and one of higher index, the conditions
for constructive and destructive
interference are reversed
30. Interference in Thin Films,
final
Be sure to include two effects
when analyzing the interference
pattern from a thin film
Path length
Phase change
31. Problem Solving Strategy
with Thin Films, 1
Identify the thin film causing the
interference
Determine the indices of refraction
in the film and the media on either
side of it
Determine the number of phase
reversals: zero, one or two
32. Problem Solving with Thin
Films, 2
The interference is constructive if
the path difference is an integral
multiple of λ and destructive if the
path difference is an odd half
multiple of λ
The conditions are reversed if one of
the waves undergoes a phase change
on reflection
33. Problem Solving with Thin
Films, 3
Equation
1 phase
reversal
0 or 2 phase
reversals
2nt = (m + ½) constructive destructive
2nt = m
destructive constructive
34. Interference in Thin Films,
Example
An example of
different indices
of refraction
A coating on a
solar cell
There are two
phase changes
35. CD’s and DVD’s
Data is stored digitally
A series of ones and zeros read by laser
light reflected from the disk
Strong reflections correspond to
constructive interference
These reflections are chosen to represent
zeros
Weak reflections correspond to
destructive interference
These reflections are chosen to represent
ones