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Diversity of Life:
Prokaryotes
BIOL 1307
Ebeling
What feature do all cells have in common?
All cells contain:
● DNA
● RNA
● proteins
● ribosomes
● cytoplasm
● cell membrane
All cells are relatively small in
size
Cell Size
Microscopy
Size matters…
● Surface is the primary point of exchange between the cell and its environment
(and the organism and its environment)
o Diffusion/Osmosis
o Heat balance
o Elimination of wastes
● As a cell increases in size, its volume increases at a faster rate than its surface. At
some point, a cell is too big to effectively accommodate the exchanges it makes
with its environment.
Surface Area to Volume Ratio
REALLY matters
insert figure 3.5
Fig. 3.5Section 3.2
Cell types
Prokaryotes vs. Eukaryotes
Prokaryote Eukaryote
Prokaryotes
Domain Archaea
Carl Woese 1977
Extremophiles
Archaea Bacteria
Bacterial Structure
Prokaryote Cell Shapes
Gram Staining of Bacteria
Hans Gram 1884
External Structures
Endospores
Capsule
Glycocalyx Pili Flagella
Prokaryote Nutrition and
Metabolism
Classification based on oxygen requirements
obligate aerobes:
REQUIRE O2
facultative anaerobes:
live either with or
without O2
obligate
anaerobes: DIE in
O2
Habitats with oxygen Habitats without oxygen
Fig 17.8
Prokaryotes and their habitats
Reproduction:
-Binary fission
Horizontal gene
transfer
Fun Stuff
How do antibiotics work?
• Inhibit cell wall formation
• Penicillin
• Disrupt cell membranes
• Inhibit protein synthesis
• streptomycin
• Inhibit transcription
• Inhibit metabolic enzymes
• Sulfa drugs
Benefits from Prokaryotes
Escherichia coli
• DNA is the genetic material
• Genetic exchange
• DNA replication
• Gene regulation
• Transgenic technology
• Gene function
https://www.ted.com/talks/rob_knight_how_our_microbes_make_
us_who_we_are?language=en
Normal Flora vs. Transient Flora
1307 prokaryotes blank  f2019

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1307 prokaryotes blank f2019

Editor's Notes

  1. Have students answer this question before revealing commonalities. All cells contain DNA, RNA, proteins, ribosomes, cytoplasm, and cell membrane. [may need to define ribosomes--cell machinery that makes proteins.] All cells are relatively small in size.
  2. As cells increase in size it is more difficult to exchange heat with the environment (which is important for thermoregulation), obtain nutrients, get rid of waste, bring in oxygen, and get rid of carbon dioxide. These items enter or leave a cell through its surface. As a cell increases in size, its volume increases more dramatically than its surface area. At some point, a cell would be too big to effectively exchange these things with its environment.
  3. This figure shows the three domains of life and compares them according to following four criteria: 1) the absence or presence of a nucleus 2) the absence or presence of membrane bound organelles,3) differences in membrane chemistry, and 4) typical cell size.
  4. Some organisms, obligate aerobes, require oxygen to survive. Oxygen plays a critical role in some of their metabolic pathways, specifically cell respiration which helps them obtain energy. [Note: we won’t discuss cell respiration until BIO 1482 so I am deliberately not trying to use the term here. However,  it may help some students connect these terms to previous knowledge about cell respiration, fermentation, and anaerobic respiration.] They will die in the absence of oxygen. However, not all living things require oxygen to survive. Facultative anaerobes, can live with or without oxygen using either. Obligate anaerobes live in environments that lack oxygen and will die in the presence of oxygen.
  5. Nitrogen fixation, cheese, insulin, activated sludge