This document provides an introduction to the principles of biology. It begins with questions students may have about the class. It then defines what science and biology are and discusses the importance of scientific literacy. The document outlines the key characteristics of living things and the three domains that make up the tree of life. It introduces the scientific method and discusses how scientists study the natural world through experiments that test hypotheses. The summary concludes by defining science as understanding nature through objective evidence, measurements, and reproducible experiments.