1) Phytase increases the digestibility and availability of calcium (Ca) and phosphorus (P) by breaking down phytate. Excess dietary Ca can negatively impact average daily feed intake, average daily gain, and feed efficiency.
2) Minimum Ca requirements are needed to maximize absorption in the small intestine and regulate absorption in the kidney. The requirements to maximize bone ash and mineralization are greater than to maximize growth performance alone.
3) Ratios between standardized total tract digestible (STTD) Ca and STTD P should be less than 1.35:1 for pigs under 50 kg, less than 1.25:1 for pigs from 50 to 100 kg, and less than 1
As part of the IFPRI Egypt Seminar in partnership with WorldFish: “Leveraging Aquaculture and Fisheries for Improving Food and Nutrition Security in Egypt”
Introduction
The mineral content in animal body is 2-5%.
• Most abundant minerals in
body:
– 36-39% Ca (bone ash)
– 17-19% P (bone ash)
Conclusion
STTD Ca requirements for 11 to 25 kg pigs:
– ADG is between 0.36 and 0.56%, G:F is 0.43%
– Bone ash, bone Ca, and bone P is between 0.48 and 0.56%
– Ca retention and P retention is between 0.48 and 0.52%
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More presentations at http://www.swinecast.com/2014-leman-swine-conference-material
Dr. Joel DeRouchey - Feed Price Update and Daily Feed Efficiency Drivers John Blue
Feed Price Update and Daily Feed Efficiency Drivers - Dr. Joel DeRouchey, Kansas State University, from the 2013 World Pork Expo, June 5 - 7, 2013, Des Moines, IA, USA.
More presentations at http://www.swinecast.com/2013-world-pork-expo
Dr. Brian Richert - Alternative Feed Ingredients: Real Options or Just a Nice...John Blue
Alternative Feed Ingredients: Real Options or Just a Nice Idea? - Dr. Brian Richert, Associate Professor of Animal Sciences, Department of Animal Sciences, Purdue University, from the 2012 Minnesota Pork Congress, January 18-19, Minneapolis, MN, USA.
As part of the IFPRI Egypt Seminar in partnership with WorldFish: “Leveraging Aquaculture and Fisheries for Improving Food and Nutrition Security in Egypt”
Introduction
The mineral content in animal body is 2-5%.
• Most abundant minerals in
body:
– 36-39% Ca (bone ash)
– 17-19% P (bone ash)
Conclusion
STTD Ca requirements for 11 to 25 kg pigs:
– ADG is between 0.36 and 0.56%, G:F is 0.43%
– Bone ash, bone Ca, and bone P is between 0.48 and 0.56%
– Ca retention and P retention is between 0.48 and 0.52%
Dr. Erin Harris - Feeding Strategies for Dried Distillers Grain with Solubles...John Blue
Feeding Strategies for Dried Distillers Grain with Solubles with Immunologically Castrated Pigs-Considerations for Producers and Packers - Dr. Erin Harris, University of Minnesota, from the 2014 Allen D. Leman Swine Conference, September 15-16, 2014, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2014-leman-swine-conference-material
Dr. Joel DeRouchey - Feed Price Update and Daily Feed Efficiency Drivers John Blue
Feed Price Update and Daily Feed Efficiency Drivers - Dr. Joel DeRouchey, Kansas State University, from the 2013 World Pork Expo, June 5 - 7, 2013, Des Moines, IA, USA.
More presentations at http://www.swinecast.com/2013-world-pork-expo
Dr. Brian Richert - Alternative Feed Ingredients: Real Options or Just a Nice...John Blue
Alternative Feed Ingredients: Real Options or Just a Nice Idea? - Dr. Brian Richert, Associate Professor of Animal Sciences, Department of Animal Sciences, Purdue University, from the 2012 Minnesota Pork Congress, January 18-19, Minneapolis, MN, USA.
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More presentations at http://www.swinecast.com/2016-leman-swine-conference-material
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More presentations at http://www.swinecast.com/2016-leman-swine-conference-material
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Overview of the Recent Amino Acids Work in Growing Pigs - Anne Clark, Kansas State Research and Extension, from the 2016 Allen D. Leman Swine Conference, September 17-20, 2016, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2016-leman-swine-conference-material
Dr. Steve Dritz - Latest Field Research and Available Tools to Evaluate and I...John Blue
Latest Field Research and Available Tools to Evaluate and Improve Feed Efficiency - Dr. Steve Dritz, College of Veterinary Medicine, Kansas State University, from the 2016 Allen D. Leman Swine Conference, September 17-20, 2016, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2016-leman-swine-conference-material
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More presentations at http://www.swinecast.com/2015-leman-swine-conference-material
The ATP binding cassette (ABC) transporter ABCG5/ABCG8 mediates sterol excretion in liver and intestines. Mutations inactivating either ABCG5 or ABCG8 cause sitosterolemia, a rare autosomal recessive genetic disorder characterized by hypercholesteromelia and premature atherosclerosis. We crystallized human ABCG5/ABCG8 in lipid bilayers and solved an X-ray structure. ABCG5/ABCG8 is the first ABCG transporter structurally characterized at atomic resolution and presents a new transmembrane fold for the ABC transporter superfamily. It shows the asymmetric nucleotide-binding sites interacting with the transmembrane domain through a conserved polar network, where the coupling-connecting helices are structurally asymmetric. The structure reveals new features of sterol transport at the bilayer-transporter interface, allows us to propose a sterol transport model and provides a new framework to study active lipid transport and to model other ABC transporters. This presentation share slides that describes the background of the physiology, protein purification, structural determination and interpretation of human ABCG5/ABCG8.
Event Report - SAP Sapphire 2024 Orlando - lots of innovation and old challengesHolger Mueller
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Personal Brand Statement:
As an Army veteran dedicated to lifelong learning, I bring a disciplined, strategic mindset to my pursuits. I am constantly expanding my knowledge to innovate and lead effectively. My journey is driven by a commitment to excellence, and to make a meaningful impact in the world.
1. Thursday Presentations prof hans stein uni of illinois - why we must use desgestible p and calcium in formulations
1. WHY WE MUST USE
DIGESTIBLE CA AND P
IN FORMULATIONS
Hans H. Stein
Division of Nutritional Sciences; Department of Animal Sciences
University of Ilinois
http://nutrition.ansci.ilinois.edu
2. 4
Excess dietary Ca is negative for ADFI, ADG and G:F
3
2
Phytase increases digestibility of Ca and P
1
Excess Ca reduces P digestibility
Take Home Messages
Phytase effect depends on substrate and ingredient
14. STTD of Ca, %
Ca level: P = 0.86; Phytase: P < 0.01; Ca × phytase: P = 0.55
48.2
68.8
0
20
40
60
80
0 0.1 0.2 0.3 0.4
Canola meal without phytase Canola meal with phytase
González-Vega et al., 2013
Ca conc., %
28. Lumen Blood
Adapted from Christakos et al., 2014.
TRPV6 ATP2B1S100G
ZO1
CLDN1
OCLNLow Ca diet
High Ca diet
Transcellular
pathway
Paracellular
pathway
Intestinal Ca absorption
29. Material and Methods
Total Ca, % 0.21 0.45 0.70 0.94 1.19
STTD Ca, % 0.14 0.29 0.44 0.59 0.74
STTDP,%
0.16 0.88:1 1.81:1 2.75:1 3.69:1 4.63:1
0.33 0.42:1 0.88:1 1.33:1 1.79:1 2.24:1
0.42 0.33:1 0.69:1 1.05:1 1.40:1 1.76:1
0.50 0.28:1 0.58:1 0.88:1 1.18:1 1.48:1
Experimental diets
STTD Ca : STTD P
30. Material and Methods
640 Pigs
4 pigs / pen (2♂ – 2♀)
Growth
performance
Bone characteristics
Right femur
21
days
Duodenal tissue
Gene expression
31. Transcellular Ca absorption related genes
0.200
0.320
0.440
0.560
0.680
0.800
0.14 0.29 0.44 0.59 0.74
STTD Ca, %
TRPV6
GE = a + (b × Ca) + (c × Ca2) + (d × P) + (e × P2) + (f × Ca × P) +
(g × Ca2 × P) + (h × Ca × P2) + (i × Ca2 × P2)
0.800
0.920
1.040
1.160
1.280
1.400
0.14 0.29 0.44 0.59 0.74
STTD Ca, %
S100G
TRPV6 = Brush border channel S100G = Ca-binding protein
P < 0.05 P < 0.05
Lagos, 2018
32. GE = a + (b × Ca) + (c × Ca2) + (d × P) + (e × P2) + (f × Ca ×
P) + (g × Ca2 × P) + (h × Ca × P2) + (i × Ca2 × P2)
Paracellular Ca absorption related genes
0.350
0.410
0.470
0.530
0.590
0.650
0.14 0.29 0.44 0.59 0.74
STTD Ca, %
OCLN
0.350
0.410
0.470
0.530
0.590
0.650
0.14 0.29 0.44 0.59 0.74
STTD Ca, %
ZO1
P < 0.05 P < 0.05
Tight junction integral membrane protein Tight junction integral peripheral protein
Lagos, 2018
33. Average daily gain, g
230
330
430
530
630
730
0.14 0.29 0.44 0.59 0.74
STTD Ca, %
0.16
0.33
0.42
0.50
STTD P, %
P < 0.01
Lagos, 2018
45. Average Daily Feed Intake, kg
ADFI = 3.6782-1.2722 × STTD Ca
0.5
1.5
2.5
3.5
4.5
0.08 0.18 0.29 0.38 0.49
STTD Ca, %
Merriman et al., 2017
46. Average Daily Gain, kg
ADG = 1.2141 – 0.0635 x STTD Ca
0.80
0.90
1.00
1.10
1.20
0.08 0.18 0.29 0.38 0.49
STTD Ca, %
Merriman et al., 2017
47. 2
Phytase
increases STTD
of Ca
1
STTD Ca available
for most
ingredients
02
3
Minimum
regulation of Ca
absorption
Conclusions
4
Regulation of
Ca mainly in
kidney
48. Excess Ca
reduces ADG
and G:F
Most
negative
effects if
STTD P is
marginal
Req. to
maximize
bone ash is
greater than
to maximize
gain
Conclusions, cont.
49. Body weight range, kg
11 to 25 25 to 50 50 to 85 100 to 130
Growth performance < 1.40:1 < 1.35:1 < 1.25:1 < 1.10:1
Bone ash ̴ 1.70:1 ̴ 1.80 ̴ 2.0:1 ̴ 2.30:1
Requirements for STTD Ca:STTD P by growing pigs STTD P
at the requirement
González-Vega et al., 2016b; Merriman et al., 2017; Lagos, 2018
Overall conclusions
Lagos, 2018
50. Recommendations
Pigs <50 kg:
Ratio between
STTD Ca and
STTD P:
< 1.35:1
Pigs from 50
to 100 kg:
Ratio
between
STTD Ca and
STTD P:
< 1.25:1
Pigs > 100 kg:
Ratio
between
STTD Ca and
STTD P:
< 1.10:1