Enzyme Technology... What the Future of Innovation looks like.

1. ENZYME TECHNOLOGY
WHAT THE FUTURE OF INNOVATION LOOKS LIKE
Amir E. Ghane, Ph. D. , PAS.
Danisco Animal Nutrition and Health (IFF)

2. Increased
demand
for protein
Optimal management
of the breeding and
starter phases
CHALLENGES
OF ANIMAL
PRODUCTION IN
THE 21ST CENTURY
Lower environmental
impact and
reduced waste
Maintaining
production in a
context of reduced
use of antibiotics
Food safety in a
context of increasing
consumer concerns
Improved
animal welfare
conditions
Feed cost reduction
allowing maximum
profitability
©2021 Property of IFF Inc.

3. SUSTAINABLE ANIMAL PRODUCTION
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Various frameworks & high targets adds pressure across the industry

4. HEADWINDS FOR MORE SUSTAINABLE PRODUCTION
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Unpredictable
opportunistic disease
challenges are increasing
Perceived 50%hit
rate on most alternatives
to antibiotics
Raw material prices
and production/supply
costs threaten feed
quality

5. NEW FEEDING STRATEGIES ARE NEEDED
for improved sustainability and to combat unprecedented price pressures and
increased volatility post Covid-19
©2022 Property of IFF Inc. – Confidential Information
5
Quarterly price forecast of feed grains
(Q1 2015 – Q1 2023f)
Source: Global Pork Quarterly Q2 2022, April 2022, Rabobank
Rock Phosphate prices
(Jun 2018 – Jul 2022)
Source: https://ycharts.com/indicators/morocco_phosphate_rock_price August 2022

6. NEW AREAS OF ENZYME RESEARCH
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6
Move to more
complex diets
Use of cheaper and
local ingredients
Can we replace soy?
Low protein diets
Phytase
use
Development of robust
matrix values
Is formulating without
inorganic P possible?
Combining
technologies
Better understanding of
potential health benefits
of enzymes (Prebiotic
effects)

7. PHYTASE AMINO ACID MATRIX MODELS
7
Dersjant
–Li
et
al.,
2022.
Modeling
improvements
in
ileal
digestible
amino
acids
by
a
novel
consensus
bacterial
6-phytase
variant
in
broilers.
Poultry
Science
101:
101666
G
O
L
D
lobally representative
riginal and based on actual feed formulation
arge number of trials supplied data for 18 amino acids
erived from basal AA improvement corrected for syn AA
©2021 Property of IFF Inc.
First to publish full models in peer reviewed journal paper.
https://doi.org/10.1016/j.psj.2021.101666

8. Sustainable
broiler production
 Broiler meat uses millions of tons
of inorganic P
 Each ton emitting ~ 750 kg CO2
 Maximizing the utilization of plant
based P resource to reduce
pollution
Challenges in total
removal of inorganic P
in broiler production
CAN WE RAISE BIRDS WITH NO INORGANIC PHOSPHORUS?
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8
AvP requirement starter :
0.48% (Aviagen)
AvP in basal diets: 0.15%
0.33% AvP needs
to be released by
phytase

9. BROILER PRODUCTION WITHOUT ADDED INORGANIC P
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9
meta-analysis of 9 datasets from 6 trials (2 datasets are simple corn/SBM diets)
[CELLR
ANGE]
[
2000
2200
2400
2600
2800
3000
3200
Day 35 Day 42
BW
(g)
Control IPF+PhyG
[CELLR
ANGE]
[CELLR
ANGE]
[CELLR
ANGE]
[CELLR
ANGE]
1.36
1.41
1.46
1.51
1.56
1.61
1.66
Up to day 35 Up to day 42
FCR
(g/g)
Control IPF+PhyG
[CELL
RANG
E]
[CELL
RANG
E]
[CELL
RANG
E]
[CELL
RANG
E]
0
10
20
30
40
50
60
Day 21 Day 42
Tibia
Breaking
Strength
(kgF)
Control IPF+PhyG

10. APPROACH TO VEGETABLE 100% INORGANIC P FREE
DIETS
Approaches
Highly efficient phytase:
Breakdown 85-90% IP6
Stimulate gizzard
development
Sufficient phytate-P
(by using phytate-rich ingredients)
Optimize calcium level
(based on limestone solubility)
Phase-specific phytase
dosing strategy
©2021 Property of IFF Inc.

11. Soy is a wonderful ingredient.
So why look at replacing it at all?
 Tendency to reduce transport
and CO2 (LULUC)
 Small but growing segment in
NW Europe: soy free demand
 Using alternative ingredients is
often more economic
Literature studies have shown
 Partial replacement of SBM was
successful
 Total replacement of SBM
reduced performance &
increased costs
©2022 Property of IFF Inc.
11
CAN WE FACILITATE SOY REPLACEMENT WITH USE OF
ENZYMES AND OTHER ADDITIVES?

12. LIMITATIONS OF ALTERNATIVE INGREDIENTS:
12
0
20
40
60
80
100
AID Lys AID Met AID Thr AID Trp
AID AA in SBM, RSM, SFM
SBM RSM SFM
 Increased fiber content
 Lower AA digestibility
 Anti-nutritional factors

13. 13
SOY FREE DIETS PROOF OF CONCEPT TRIAL
Trial design
Number: 572 | Type: Ross 308| Sex: male
Treatments: 2 | Replicates: 13| Birds per pen: 22 |
Diets: corn/soybean meal vs low protein soy-free
Form: pelleted | Trial length: 1-42 days
DIETS
SBM
(PC) * ◊
ALT2d
L CP
CP Level, % -2 to 1.5%
Phytase, FTU/kg
• Starter 2000 2000
• Grower 1500 1500
• Finisher 1000 1000
Xylanase ◊, XU/kg 750 -
XB**, Xylanase U/kg - 1200
Protease ◊, U/kg - 800
Probiotics, CFU/g - 150000
XB = xylanase + Beta-glucanase, providing 152 unit of Beta-glucanase
Starter (1-10 D) Grower (10-21 D) Finisher 1 (21-35 D) Finisher 2 (35-42 D)
Nutrients, % SBM (PC)
ALT2 Low
CP
SBM (PC)
ALT2 Low
CP
SBM (PC)
ALT2
Low CP
SBM (PC)
ALT2
Low CP
ME - Poultry 2950 2950 3050 3050 3100 3100 3150 3150
Crude Protein 23.5 21.5 22.0 20.0 20.2 18.2 19.3 17.8
Dig Lys 1.22 1.22 1.11 1.11 0.99 0.99 0.93 0.93
Syn Lys/Total Lys, %* 13 29 15 34 17 39 20 37
Crude Fiber 3.4 6.0 3.9 6.3 4.1 5.7 4.5 5.8

14. ENZYMES PLUS PROBIOTIC GAVE SIMILAR PERFORMANCE
TO BREED SPECS IN LOW PROTEIN SOY-FREE DIETS
325a
1111a
2492a
3256a
308b
1035b
2324b
3082b 3136
d10 d21 d35 d42
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14
Bodyweight (g) FCR
1.08b
1.29b
1.46b
1.57b
1.13a
1.35a
1.53a
1.61a
1.60
d1-10 d1-21 d1-35 d1-42

15. HIGH DOSE XYL (XAP) & PHYTASE IMPROVE PERFORMANCE
FCR (g/g, 1-42 days)
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15
in diets reduced by 120 kcal/kg AME and 3% Crude protein
European Production Index (1-42 days)
XAP at 100g/t provides 2000 U xylanase, 200 U amylase and 4000 U protease/kg feed
XAP at 200g/t provides 4000 U xylanase, 400 U amylase and 8000 U protease/kg feed
1.580ab
No XYL
1.557a
100 GXYL
1.565ab
200g XYL
1.594ab
1.599b
1.572ab
1.577ab 1.577ab
1.576b
0 FTU
2000 FTU
1000 FTU
No XYL
393.5a
100g XYL
414.7abc
432.5c
200 g XYL
412.2abc
398.7ab
428.4bc
423.0abc
420.0abc
434.0c

16. SERUM BIOMARKERS GIVE INSIGHTS INTO ENZYME ACTION
Peptide YY(pg/mL) Uric acid (mmol/L)
2.87a
2.59a
100g XYL
No XYL 200 XYL
4.25b
3.25ab
3.40ab
2.52a
2.51a 2.52a
2.50a
0 FTU
1000 FTU
2000 FTU
XAP at 100g/t provides 2000 U xylanase, 200 U amylase and 4000 U protease/kg feed
XAP at 200g/t provides 4000 U xylanase, 400 U amylase and 8000 U protease/kg feed
No XYL 100 g XYL
98.8a
116.7ab
2oo g XYL
162.9c
115.7ab
130.3b
105.6ab
92.2a
130.4b
98.8a
High inclusion levels of phytase and XAP decrease uric acid and peptide YY in circulation
Lower uric acid in serum indicates
greater utilization of dietary protein
Peptide YY helps limit feed intake.
Lower levels mean birds can eat more

17. RECOMMENDING TECHNOLOGY COMBINATIONS IS A SCIENCE
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17

18. EXOGENOUS ENZYMES PLAY A ROLL IN HEALTH & PERFORMANCE
©2022 Property of IFF Inc.
18
Short arabinose xylose sugars (AXOS)
Prebiotic effect (SCFA production)
Improved digestion
AMINO ACIDS
FAT
STARCH
Xylanase
Amylase
Protease
Nutrients Locked by
Fibre, reduce viscosity Undigested Nutrients, reduce protein reaching
distal gut and avoid NE blooms

19. LESS ABOUT WHO IS THERE AND MORE ABOUT WHAT THEY
ARE DOING… CROSS FEEDING
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19
1,4 Arabinoxylan
HYDROLYSIS
Short chain Arabino-
oxyloligosaccharides
(AXOS)
SCFA Lactate
Butyric acid
Acetic acid
Gases
Water
molecules
DP2-0.3 DP3-0.25 DP4-0.20
Utilised by bacteria in the caeca

20. SYNCRA® GIVES SYNERGISTIC ENERGY
IMPROVEMENTS VERSUS THE COMPONENTS
Copyright © 2022 IFF. All rights reserved
20
Predicted vs measured ileal digestible energy improvement vs control (kcal/kg DM)
SYNCRA.
TR
4
Massey
University
538 Male Ross 308 broilers, Corn/SBM/7% DDGS/6.7% wheat midds
21 day trial, Results are versus an unchallenged control
abc P<0.05
IFF Probiotic IFF Enzyme Syncra®
34
60 63
6
21
22
10
11
25
50
92
110
Predicted Energy
from Starch
Predicted Energy
from Fat
Predicted Energy
from Protein
SYNERGY
Measured IDE
34b
74b
166a
4.2 kcal/g starch;
5.5 kcal/g protein;
9.4 kcal/g fat

21. Copyright © 2022 IFF (Danisco Animal Nutrition & Health). All rights reserved. IFF™, the IFF logo and all products denoted with ® or
™ are trademarks of the affiliates of IFF. The information contained herein is subject to change without further notice, is provided at
the request of and without charge to our customers and is based upon data IFF believes to be reliable. Conditions and methods of use
of the products are beyond our control, and nothing contained herein shall be construed as a representation or warranty on use or of
non-infringement of intellectual property, regulatory or legal compliance. If this presentation is provided in any language other than
English, the original English version will control, and IFF hereby disclaims responsibility for any errors caused by translation.