Productivity and Performance in Monogastrics with postbiotics.

1. This document is provided to you, at your request, for informational purposes only. These materials do not, and are not intended to, constitute veterinary,
legal or regulatory advice. The information contained in this document is based on publicly available sources and/or unpublished data and is believed to
be true and accurate, but Diamond V does not guarantee or make any warranty of accuracy or completeness. Legal and regulatory compliance for your
business is your responsibility. The purchaser/user assumes all risks relating to the use of the information contained herein, and agrees that we are not
liable to you or any third party relating to the use of such information. We recommend you consult animal health, regulatory and legal advisors familiar
with all applicable laws, rules and regulations.
Diamond V
Monogastric
Update
Dr Curtis Harms DVM
Feedworks Conference
September 14, 2022

2. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
What’s the Difference – Pre-, Pro-, and
Postbiotics
2

3. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Meeting the Genetic Potential
3
Genetics
Bird Welfare
Vaccination
Program
Nutrition
Stocking
Density
Lighting
Ventilation
Immune System
Temperature
Water Quality
Chick
Quality Feed Supply
Housing
Overall Health
Breeder Age
Feed Form
Management
Biosecurity
Pathogens
Stress

4. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Layer Performance
Highlights:
 Postbiotic: Stable, all natural, fermented product containing functional
bioactive metabolites
Original XPC™
 In Pullets: 2.5 lbs/ton or 1.25 kg/MT
 In Lay Phase: 1.5 lbs/ton or .75 kg/MT
XPC Ultra™
 In Pullets: 1.25lbs/ton or .625kg/MT
 In Lay Phase: .75lbs/ton or .375kg/MT
4

5. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Diamond V Laying Hen Research
Observed Effect of XPC on Laying Hen
Performance
Egg Production Improved
Feed Efficiency Improved
Mortality Improved
Egg Size Improved
Egg Quality Improved
Egg solids Improved
5
Sources:
60+ field trials
North Caroline State University
 Random sample
 18 layer strains
A Solid Production Based
ROI of ≥3:1

6. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Objective
To determine the effect of XPC on performance of commercial
laying hens
 Conventional housing:
 38 Matched Flocks:
 CON: 3,440,257
 XPC: 3,384,109
 Cage-Free housing:
 19 Matched Flocks:
 CON: 285,278
 XPC: 281,074
6

7. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Hen Day Production (%)
Weighted Average
7
85.94
88.15
84.5
85.5
86.5
87.5
88.5
89.5
Conventional
87.73
89.43
84.5
85.5
86.5
87.5
88.5
89.5
Cage-Free
2.21 1.70
CON:3,440,257 XPC: 3,384,109 CON: 285,278 XPC: 281,074

8. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Feed Conversion (lb. feed/doz. Eggs)
Weighted Average
8
3.24
3.16
2.9
3.1
3.3
3.5
Conventional
3.47
3.38
2.9
3.1
3.3
3.5
Cage-Free
CON: 3,440,257 XPC:3,384,109 CON: 285,278 XPC: 281,074
8 point
Advantage
9 point
Advantage

9. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Mortality (%)
Weighted Average
9
3.98
3.57
0
2
4
6
8
10
Conventional
9.68
8.92
0
2
4
6
8
10
Cage-Free
0.76
CON: 3,440,257 XPC: 3,384,109 CON: 285,278 XPC: 281,074
0.41

10. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Observation: 57 Field Trials
93% of XPC flocks…
Measured benefit in 1 or more traits
10
Benefits
No measured benefit 4 flocks 7%
1 trait 11 flocks 19%
2 traits 24 flocks 42%
All 3 traits 18 flocks 32%

11. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Broilers
Proof:
 Mississippi State University
 Auburn University
 Texas A&M University
 North Caroline State University
 University of Georgia
 University of Arkansas
 Southern Poultry Research
 Colorado Quality Research
 USDA / ARS
 Commercial Trials (Confidential Customer Evaluations
11
Production Response
BREEDERS
Egg Production Improved
Feed Efficiency Improved
Fertility Improved
Hatchability Improved
Chick Quality Improved
Progeny Performance Improved
COMMERCIAL BIRDS
Weight Gain Improved
Feed Efficiency Improved
Livability Improved
Uniformity Improved
Carcass/Breast Meat Yield Improved
Production Response ROI 5:1

12. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
12
Broiler Live Performance
Results
Commercial Field Testing

13. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Broiler Feed Conversion:
Performance Summary
13
-0.083
-0.025
-0.001
-0.098
-0.030
-0.024
-0.044
-0.024
-0.033
-0.040
-0.120
-0.100
-0.080
-0.060
-0.040
-0.020
0.000
Adjusted
FCR
Advantage
(points) BC02B BC03 BC07 BC09 BC10 Average
BC14
BC05

14. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Cobb Breeder: 24-39 Weeks
Mississippi State University
14
a,bP<0.05
Kidd et al, 2013 (JAPR)
 Hen-day Egg Production
69.5b
67.3b
81.4a
74.4a
50
60
70
80
90
24-31 W 31-39 W
Percent
Control XPC
Hatchability: Eggs Set
82.9b
81.3b
88.2a
84.3a
75
80
85
90
95
24-31 W 31-39 W
Percent
Control XPC

15. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Cobb Breeder: Egg Production
Auburn University
15
* Weekly Significant Difference (P < 0.05)
Berry et al, 2010
*
*
*
%
P = 0.21
*
*
*
Treatment Average
(26-55wk)
# Eggs/Hen
(26-55wk)
Control 65.5% 137.8
XPC 67.0% 140.7 (+3)

16. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Broiler Breeder Farm
Ross 708 - ONT
16
2015, Broiler Breeders; Ross 708; ONT-CAN
0
20
40
60
80
100
26 30 34 38 42 46 50 54
Egg
Production
(%)
Age (Wks)
% EP to 57 weeks
XPC AVG CON
TRT % EP EPH (total)
AVG
FERT (%)
AVG %
HATCH
Avg. Con 60.96 136.5 94.13 84.32
XPC 63.98 143.3 95.30 85.65

17. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Proven Advantages
Reproduction
 Livability
 Egg numbers
 Egg quality
 Fertility
 Hatch
 Poultry quality
Health
 Mortality
 Enteric strength
 Pathogen reduction
 Virus protection
 Respiratory challenge
 Vaccine titers
17
Food Safety
 Prevalence
 Load
 Virulence
 Resistance
Performance
 Weight gain
 Feed conversion
 Livability
 Condemns
 Breast meat
 Muscle quality

18. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
18
XPC for Sows

19. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Researched Benefits in Sows
19
Performance
 Increased number of pigs weaned (SW021)
 Decreased piglet mortality (SW021)
 Improved litter weaning weight (SW021,
SW014)
 Reduced weight variation (SW021, SW130)
 Reduced wean to estrus interval (SW014)
Immune Strength
 Reduced Clostridium perfringens
concentrations in sow and piglet (SW020)
 Decreased serum neutrophil concentration
during gestation/lactation (SW016)

20. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Heavier Litter Weaning Weight and #
20
XPC Advantage Compared to Control
3.06 kg average improvement across 10 studies
0
2
4
6
8
10
12
SW003 SW007 SW013 SW014 SW016 SW017 SW019 SW021 SW022 SW023
Increase
in
Litter
Weaning
Wt,
kg
XPC Advantage Compared to Control
0.41 average improvement across 10 studies
0.00
0.20
0.40
0.60
0.80
1.00
1.20
SW003
SW007
SW013
SW014
SW016
SW017
SW019
SW021
SW022
SW023
Additional
Pigs
Weaned,
#

21. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Materials and Methods
48 sows were evaluated for farrowing and weaning
performance
Treatments were administered via feed
 Control diet – no antibiotics or probiotics, formulated to EU standards
 Control diet + 2kg/MT Original XPC
Genetics
 Sows were Danbred maternal
 Progeny were composed of Danbred (♀) x Pietrain (♂)
Parity was distributed equally across treatments
Sows were given a chance to nurse 12  1 piglets
21

22. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Nursedam Performance on Piglets in
Lactation
Treatment BW, Birth
kg
BW, 28 d of Ageb
kg
ADBbc
g/d
CVb
%
PWMb
%
Number
Weaned
Control 1.30 7.61 243.0 17.35 5.54 11.30
XPC 1.27 8.20 259.0 16.66 1.75 11.78
SEM 0.01 0.07 2.77 1.14 - 0.15
P NS <0.0001 <0.0001 NS 0.016 0.03
b = random effect of room
c = covariate of birth weight
N = 48 (24 litters/treatment) – nurse sows given a chance to nurse 12 ± 1
SEM = pooled standard of error of mean
BW = body weight
ADG = average daily gain – grams per day
ADFI = average daily feed intake
PWM = pre-wean mortality
NS = not significant, P>0.05
22

23. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Results
23
 Sows fed Original XPC during gestation and lactation improved
weaning weight, number weaned, and pre-weaning mortality.
 ROI of using Original XPC is more than 5-fold (average results)
 Number weaned
 4.5 litters lifetime x 0.30 = 1.35 more pigs weaned
 1.35 x $35 weaned pig value = $44.41
 Weaning weight
 0.45 kg at weaning equates to 1.36 – 1.81 kg advantage at market
 Equates to $1.61 – 2.15 per pig marketed, assuming $1.65 per kg
 Not significant, but sows fed Originial XPC tended to hold body
condition score better than sows fed a control diet as well as return
to estrous quicker and a higher conception rate.
(Further investigations are in the pipeline to evaluate this)

24. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
24
XPC for Grow/Finish

25. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
XPC Ultra Grow/Fin – Brazil 2021
 Commercial research facility
 10 pens per treatment with 11 pigs per pen
 Treatments evaluated:
 Control
 Control + XPC Ultra (0.5kg/MT)
 Control + XPC Ultra (1.0kg/MT)
*2 other experimental treatments were also evaluated
 Gilts and immunocastrates were penned separately
 Trial was for 100 days in grow/fin
 Overall, the trial had clean health (very little mortality or morbidity)
25

26. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Growth and Performance Measurements
of Pig
Trait Control Control + XPCU
(0.5 kg/MT)
Control + XPCU
(1.0 kg/MT)
P-value
Initial Wt (kg) 26.97 26.65 26.79 0.921
End Wt (kg) 134.48 135.57 133.76 0.765
ADG 1.086 1.093 1.080 0.619
End Wt Adj* (kg) 136.65 137.46 135.72 0.763
FCR 2.29a 2.23b 2.21b 0.033
26
*End Weight was adjusted to 101 days in finishing

27. © Diamond V, Inc. All rights reserved. Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use
Table 2: Carcass Traits of Pigs
Trait Control Control + XPCU
(0.5 kg/MT)
Control + XPCU
(1.0 kg/MT)
P-value
Hot Carcass Wt
(kg)
97.06 97.06 96.27 .909
Carcass Yield (%) 70.92 70.83 71.15 .568
Backfat (mm) 15.62 14.69 14.52 0.052
Loin Depth (mm) 65.26a 67.80b 57.77b 0.004
Lean Meat (%) 57.21a 58.23b 58.361b <0.001
27

28. © Diamond V, Inc. All rights reserved.
28
Key Take HOME:
• More Egg Production / More Pigs
• Improved Feed Efficiency
• Improved Mortality -
• Improved Progeny Performance
• Weight Gain – Broiler / Pigs
• Low Risk – Profitable ROI
• Consistent Results – repeatable
Confidential — Feedworks Conference September 2022 — Not For Distribution or Commercial Use

29. © Diamond V, Inc. All rights reserved. All label claims must meet the laws and regulations of each state or country where the product will be used.
This presentation and the information described are not label claims or intended to imply that Diamond V products can be used to diagnose, mitigate, treat, cure, or prevent disease.