The main purpose of these slides is to convey information to the Professors, Lecturers, and Students. These slides contain authentic information about this topic which is mentioned in that.

1. EXTRACTION OF DNA FROM
GOAT LIVER
a
M.Phool Badshah

2. Principle
• DNA can be isolated from cells and tissues by high
salt or phenol extraction.
• Here, chromatin and proteins are dissociated by
treatment with SDS.
• The proteins are denatured by phenyl chloroform
treatment.
• It becomes insoluble and can be prepared by the
following chemicals.
Principle

3. Materials :
• Ethanol
• Saline
• EDTA
• pH-8.0 (0.9% NaCl and 0.01 M EDTA)
• 10% SDS
• 80% phenol
• 95% ethanol
Materials

4. A
• 2.5 gm of liver tissue was homogenized in 25 mL
of saline EDTA and transferred to a 250-mL
conical flask.
• To this, 2.5 mL of 10% SDS and 2.5 mL of 80%
phenol was added and shaken well for 10
minutes.
• The entire mixture was centrifuged at 10000 rpm
for 10 minutes. The aqueous layer was separated
out.
Procedure

5. a
• To the remaining material, (interphase +
phenol phase), equal volume of chloroform,
isoamyl alcohol (24 : 1) was added and shaken
for 25 minutes.
• It was again centrifuged at 10000 rpm for 10
minutes.
• The aqueous phase was collected.
• To the aqueous phase, equal volume of 80%
phenol was added and again centrifuged at
10000 rpm for 10 minutes.

6. a
• To the aqueous phase obtained, 95% ice cold
ethanol was added and then the flask was
shaken gently.
• The DNA are precipitated as a white mass.

7. • By the addition of ice-cold ethanol, DNA
strands were obtained as a white mass or
turbidity.
RESULTS
A