Plant tissue culture involves growing plant cells, tissues, or organs in an artificial nutrient medium outside of the intact plant. Some key events in the history of plant tissue culture include: - In the early 1900s, scientists like Haberlandt and Harrison began experimenting with callus formation and cultivating plant fragments outside of the body. - In the 1930s and 1940s, scientists like White, Braun, and Skoog pioneered cultivating plant tissues for unlimited periods and studying crown gall formation and organogenesis in tobacco callus. - Advances in the late 1980s and 1990s allowed for the production of somatic hybrids between tomato species, regeneration of fertile maize plants from protoplast

1. Plant Tissue culture
M.PhooL Badshah

2. History
• In 1756 Duharmel du Monceau H. L discovered callus formation from
elm tree.
• 1853- Trecul. A performed experiment on callus formation by decorticated
trees such as Robinia, Pawlonia and Ulmus.
• 1893- Rechinger, C described callus formation on isolated stem fragments
and root slices.
• 1901- Morgan, T.H coined the term totipotency to describe the capability
of a cell to form an individual plant.
• 1902- Haberlandt, G – Father of plant tissue culture.
• 1907- Harrison, R.G devised methods of cultivating fragments of living
nerve.
• 1910- Carrel, A was the first scientist who demonstrated the culture of
living cells outside the body of an organism.

3. vfc
• 1939- White, P.R., Gautheret, R.J. and Nobecourt, P simultaneously
announced the possibility of cultivating plant tissues for unlimited periods.
• 194l- Van Overbeek, J., Conklin, M.E. and Blackeslee, A.F established
importance of coconut milk for growth and development of very young
Datura embryos.
• 1942- White, P.R. and Braun, A.C initiated studies on crown gall and
tumor formation in plants.
• 1944- Skoog, F started his work on organogenesis in tobacco callus.
• 1986- Kinsara A., Patnaik, S.N., Cocking, E.C. and Power, J.B
produced somatic hybrids between Lycopersicon esculentum and L.
peruvianum.
• 1989- Prioli, L. M. and Sondahl, M. R recovered fertile plants from
protoplasts of maize.
• 1990- Iida, A., Seki, M. Kamada, M. YHamada Y. and Morikawa
delivered genes into cultured plant cells by DNA-coated gold particles
accelerated by a pneumatic particle gun.