Details of Bacteria isolated and exploited as Green Fertilizer.

1. Isolation of Plant Growth
Promoting Rhizobacteria
from the
Gangetic Plains of North Bihar
and its impact on the
growth of plant
Momordica charantia Linn
Under the supervision of
Dr. Ashok Kumar Ghosh
H.O.D Research
M.C.S.-Phulwari , Patna
Presented By-
Dr. Mini Tiwari
Ph.D.
A.N.College,Patna

2. Sampling Site
Gangetic Plain Of North Bihar
The GPS coordinates of the five investigation
sites-
S1 - Near GANDHI sETU
S2 - Banana field
S3 - Wheat + Mustard
field
S4 - Stagnant pond
S5 - Near Ganga River

3. Isolation and Screening of microorganism
Isolation of Microbes - Serial dilution method, Sub-culture
and Pure culture method.
Characteristics of microbes - BERGEY’S Manual of
Systematic Bacteriology.

4. Isolation and Screening of microorganism
Rank Name/Title Authors Strain Accession
Pairwise
Similarity
Diff/Total
nt
megaBLAST
score
BLASTN
score
1
Aneurinibacillus
migulanus
(Takagi et al. 19
93) Shida et al.
1996
DSM 2895(T) X94195 99.771 3/1311 2575 2575
S1(V)23-
Rank Name/Title Authors Strain
Accessi
on
Pairwise
Similarity
Diff/Total nt
megaBLAST
score
BLASTN
score
1
Aneurinibacillus
migulanus
(Takagi et al. 19
93) Shida et al.
1996
DSM 2895(T) X94195 99.931 1/1444 2849 2849
S2(V)12-
Source : IMTECH,Chandigarh

5. Pot Experiment- On Momordica chanartia Lin.( Jaunpuri Variety)
Treatments of Soil
Soil for Pot Experiments Collected and Sterilized - Garden of A.N.
College, Patna.
Five type of Soil Combination.
Control soil + Chicken manure
Control soil + Chicken manure + Chemical Fertilizer
Control soil + Chicken manure + Strain S1(V)23
Control soil + Chicken manure + Strain S2(V)12
Control soil + Chicken manure + Strain S1(V)23 x Strain S2(V)12

6. Pot Experiment- On Momordica chanartia Lin.( Jaunpuri Variety)
Propagation of Seeds
Experiment Carried out in the Month - Mid January
5x25 Seeds Inoculated for 24 hours with Ionic free
water , Chemical Fertilizer and PGPR Strains
Fruit Harvested Season – Summer
20 Days Cycle Followed for 95 Days After Sowing

7. Soil pH * A measure of the acidity or alkalinity of the soil
* Affects the availability of nutrients
SOIL ANALYSIS
Soil Electrical Conductivity * Soil Texture
* Cat ion exchange capacity(CEC)
* Organic matter level
* Crop productivity
Soil Organic Carbon * Makes up-2-10% of the soil
* Critical role in the physical, chemical and biological function
* Contributes in nutrients turn-over, moisture retention, degrada-
tion of pollutants and soil buffering.

8. Nutrients in Soil
Nutrients are chemical elements and compounds found in
the environment that plants and animals need to grow and
survive.
The Big 13
The 13 mineral nutrients - Come from soil, dissolved
in water & absorbed through plant’s roots.
Always not enough nutrients in the soil - Plant to grow
healthy.
Many farmers and gardeners use fertilizers –Add
nutrients to the soil.

9. Macronutrients:
Macronutrients are elements and compounds needed in
large quantities for a plant to grow.
The Primary Nutrients
Nitrogen(N), Phosphorous (P), and Potassium (K)- First lacking from the soil as plants
use large amounts for their growth and survival.
The Secondary Nutrients
Calcium (Ca), Magnesium (Mg)- Found enough in the soil so fertilization is not
always needed.
Sulphur(S) - Found in sufficient amount from the decomposition of organic matter.

10. Micronutrients:
Micronutrients are elements essential for plant growth but needed in
very small quantities and sometimes called trace elements.
The Micronutrients
Boron(B), Copper(Cu), Iron(Fe), Chloride(Cl), Manganese(Mn)
Molybdenum(Mo) and Zinc(Zn).

11. Analysis of pH, Salinity and Organic Carbon of Soil
Results
Analysis Scale Standard Control Chemical S1(V)23 S2(V)12
S1(V)23
X
S2(V)12
pH
( 6.5- 7.5)
7.5 7.384 6.02 7.565 7.238 7.452
EC- dsm-1
(>1.00)
0.999 0.428 0.702 0.387 0.271 0.328
OC-%
(0.5-0.95)
0.5 0.539 0.516 0.974 1.041 1.093
7.5 7.384
6.02
7.565
7.238 7.452
0.999
0.428
0.702
0.387 0.271 0.328
0.5
0.539 0.516
0.974 1.041 1.093
0
1
2
3
4
5
6
7
8
Standard Control Chemical S1(V)23 S2(V)12 S1(V)23 X
S2(V)12
pH EC- dsm-1 OC-%
Source: Analysis scale : ICAR
Standard : CSTL, Patna
Table :1

12. Figure :2
Analysis of Macronutrients – N,P,K
Nutrients Analysis Standard Control Chemical S1(V)23 S2(V)12
S1(V)23
X
S2(V)12
Phosphate
(55-95 Kg/ ha)
50 49 97 73 69 80
Nitrogen
( 250-350Kg/ka)
350 276 554 463 354 402
Potash
(125-350 Kg/ha)
300 249 579 452 372 399
Table : 2
Source: Analysis scale : ICAR
Standard : CSTL, Patna
Results
50 49
97
73 69 80
350
276
554 463
354
402
300
249
579
452 372
399
0
100
200
300
400
500
600
700
Standard Control Chemical S1(V)23 S2(V)12 S1(V)23 X
S2(V)12
Phosphate(kg/ha) Nitrogen(Kg/ha) Potash(Kg/ha)

13. Analysis of Micronutrients - Zn, Cu, Mn and Fe
Analysis Scale Standard Control Chemical S1(V)23 S2(V)12
S1(V)23
X
S2(V)12
Zn
(> .85 ppm)
1..1 .98 1.68 1.12 1.24 1.22
Cu
( > .1.2 ppm)
1.5 1.244 2.822 1.584 1.198 1.31
Mn
( > 5ppm)
5 3.679 8.931 6.927 5.552 5.828
Fe
( > 7.0 ppm)
8 8.666 12.858 9.048 8.048 9.211
Source: Analysis scale : ICAR
Standard : CSTL, Patna
Fig :3
Table : 3
Results
1.1 0.98
1.68
1.12 1.24 1.22
1.5 1.244
2.822
1.584
1.198 1.31
5
3.679
8.931
6.927
5.552 5.828
8
8.666
12.858
9.238
8.048
9.211
0
2
4
6
8
10
12
14
Standard Control Chemical S1(V)23 S2(V)12 S1(V)23 X
S2(V)12
Zinc Copper Manganese Ferrous

14. Lets sum up….

15. Acknowledgement
Dr. Sushil Kumar- Astt Dir. Chemistry, CSTL, Patna
Sri. Nashrool Kalaam, ARO, CSTL,Patna