This document discusses Cryptosporidium, a parasite that causes cryptosporidiosis disease. The parasite is protected by an outer shell that allows it to survive outside the body for long periods and tolerate chlorine disinfection. Histologically, the parasite is located within epithelial cells and can cause fusion or loss of intestinal villi. The objective is to evaluate Cryptosporidium infection in malnourished and well-nourished children and how parasitic infections are affected by nutritional status and socio-demographic factors. Material and methods include using PCR and staining techniques to identify Cryptosporidium species. Results show Cryptosporidium was detected in samples. Malnutrition increases risk of infection in children due to

1. VALENTINA PÁJARO ARANGO
3º semestre | Facultad de Medicina | Universidad Pontificia Bolivariana

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INTRODUCTION
Cryptosporidium is a
microscopic parasite that
causes the diarrheal
disease cryptosporidiosis. The parasite is protected by
an outer shell that allows it
to survive outside the body
for long periods of time and
makes it very tolerant to
chlorine disinfection.
Histologically, the
parasite is located
within the epithelial
cells, processes of
fusion or loss of
intestinal villi,
hyperplasia of the
crypts and
inflammatory changes
in the lamina propria
can occur.

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Malnutrition
is a global
problem

4. Objective
Evaluation of Cryptosporidium infection in
malnourished and well-nourished children and
how parasitic infections are affected by the
interactionof nutritional status and socio-
demographic characteristics.
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5. Materiales y métodos

6. Materiales y métodos
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✢ PCR -> esunatécnica de
amplificación enzimatica de
secuenciasespecíficas deADN
usandouna polimeraza, para
finalmenteobtenermiles o
millones decopias, enesoestudio
seusó parapoder definir qué
Cryptosporidium era.
PCR
ANIDADA

7. Materiales y métodos
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✢ TINCIÓN -> La técnica
usada fuelade Ziehl-
Neelsenqueesunatinción
utilizada paraidentificar
ácidosgrasosen
organismos.

8. Materiales y métodos
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✢ SECUENCIACIÓN -> a
partirde unasecuencia de
ADNseestablececuales
la cadenade nucleotidos
de esahebra yesto sehace
para identificar quéclase
de Cryptosporidium era.

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RESULTADOS
Cryptosporidium

10. RESULTADOS
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A: Second round nested-PCR amplification
of Cryptosporidium.
• M: 100pb DNA size marker
• +Ve/-Ve: Positive/Negative control
• Lane 1 and 2: Positive samples
• Lane 3: Negative sample
B: Gel electrophoresis of LAMP products.
• +Ve/-Ve: Positive/Negative control
• Lane 1-3: Cryptosporidium positive sam-
ples.

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RESULTADOS

12. DISCUSION
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D. Mondal
Malnutrition is a known risk
factor for infection; children,
particularly those who are
malnourished, are more
susceptible because of the
lack of acquired immunity.
B.T. Mmbaga,
S.M. Caccio
Cryptosporidiosis is related
to a longer period of diarrhea
and shows mortality rates
that are 2–3-fold higher in
young children than in older
patients.
H. Liu
A wide range of vertebrate
and invertebrate hosts are
infected by different species
of Cryptosporidium.

13. Conclusion
Cryptosporidium mainly
affectschildren and
immunocompromised
people, whether due to
underlying diseasesor
because of the
socioeconomic situation that
puts them in a vulnerable
place to acquire the disease.
The main cause of acquiring
this diseaseis due to poor
hygiene and poor
managementof human
waste,such asfeces,
and can bemanaged by
educating the society and
ensuring the good nutrition
of children.
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15. Thanks!