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Estimation of variability parameters
(Components of variance)
Estimation of PCV and GCV for replicated data
Problem : 1
Lint index of an experiment with 25 cotton genotypes is
given below. These genotypes have been evaluated in RBD
with 3 replications. Workout components of variance for lint
index.
Genotypes Replication I Replication II Replication III Total
1 36.5 36.0 36.4 108.9
2 51.0 54.0 55.5 160.5
3 42.1 44.8 42.9 129.8
4 46.5 51.0 49.5 147.0
5 49.5 48.0 47.3 144.8
6 51.0 49.5 51.5 152.0
7 50.0 49.0 49.8 148.8
8 54.5 52.0 55.5 162.0
9 50.5 48.5 52.0 151.0
10 53.8 54.0 53.5 161.3
11 57.0 56.0 55.0 168.0
12 52.5 51.0 50.0 153.5
13 47.5 48.0 48.5 144.0
14 57.0 56.0 57.5 170.5
15 39.0 37.0 40.0 116.0
16 47.5 49.0 48.0 144.5
17 60.0 61.0 62.0 183.0
18 61.0 62.5 61.5 185.0
19 46.5 48.5 45.0 140.0
20 47.0 47.5 48.5 143.0
21 54.0 55.0 55.5 164.5
22 48.0 48.5 49.5 146.0
23 47.0 45.5 47.0 139.5
24 58.5 58.0 57.5 174.0
25 55.5 57.5 56.5 169.5
Total 1263.4 1267.8 1275.9 3807.1
Estimation of variability parameters
(Components of variance)
Estimation of PCV and GCV for replicated data
Correction factor =
=
= 193253.46
Raw sum of squares = 36.52
+ …………. 56.52
= 196058.55
(Grand Total)2
N
(3807.1)2
75
( ∑ x 2 )
Total sum of squares = Raw S.S – C.F
= 196058.55 – 193253.46
= 2805.09
Treatment sum of
squares
= 108.92
+ ……. + 169.52
– C.F
= 2708.59
Sum of squares of
Treatment Total
No. of rep
=
– C.F
587886.17
3
= – 193253.46
Replication sum of squares =
Error SS = Total SS – Treat SS – Rep SS
= 93.28
Sum of squares of
Rep.Total
No. of Treatments
=
1263.42
+ …… 1275.92
25
=
– 193253.46
3.22
– C.F
Anova
Sources of
Variation
df SS
MSS
SS
df
F.ratio
Replication (r-1)
3-1 = 2
3.22 1.61 1.61
1.94
Treatment (t-1)
25-1 = 24
2708.59 112.86 112.86
1.94
Error (t-1) (r-1)
24 x 2
= 48
93.28 1.94
Total 74 2805.09
= 0.83
= 58.18
F Table
(48, 24) 5% 1.74
1% 2.20
If the calculated value is
higher than the table value, it
is significant. Treatments are
differing significantly.
Environmental variance (Ve) = EMS (Error Mean square) = 1.94
Genotypic variance (Vg) =
=
= 36.97
(Ms due to treatment – EMS)
r
112.86 – 1.94
3
Phenotypic variance (Vph) = Vg + Ve
= 36.97 + 1.94
= 38.91
GCV =
= 50.76
=
=
= 11.98%
√Vg
x
x 100
3807.1
x =
75
√36.97
x 100
50.76
6.08
x 100
50.76
PCV =
=
= 12.29%
√Vph
x
√38.91
x 100
50.76
x 100
ECV =
=
= 2.76%
< 10 - low
10 - 20 - Moderate
above 20 - High
The PCV and GCV of lint index are moderate. It means that
the variability is moderate. The influence of environment is
low.
√Ve
x
√1.94
x 100
50.76
x 100
Heritability =
=
= 95.01%
GA = K x √Vph x
= 2.06 x √38.91 x
= 2.06 x 6.24 x 0.95
= 12.21
Vg
Vph
x 100
36.97
38.91
x 100
Vg
Vph
36.97
38.91
GA as percent of Mean
=
=
= 24.05
Inference
1. If the GCV is higher than PCV, it indicates that there is little
influence of environment on the expression of character.
Selection for such character will be rewarding.
GA
x
x 100
12.21
50.76
x 100
2. If PCV is higher than GCV, it means that the apparent
variation is not only due to genotypes but also due to
influence of environment. Selection for such traits may be
misleading.
3. If ECV is higher than PCV & GCV, it means the environment
is playing a significant role in the expression of such
character. Selection for the improvement of such character
will be ineffective.
R1 R2 R3 Total
T1 65 95 84 244
T2 46 54 58 158
T3 62 68 67 197
T4 67 61 63 191
T5 75 77 71 223
T6 47 51 53 151
T7 61 66 69 196
Total 423 472 465 1360
Problem : 2
Calculate the components of variance.

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Basics of crystallography, crystal systems, classes and different forms
 

2. Estimation of variability parameters - Final.pptx

  • 1. Estimation of variability parameters (Components of variance) Estimation of PCV and GCV for replicated data Problem : 1 Lint index of an experiment with 25 cotton genotypes is given below. These genotypes have been evaluated in RBD with 3 replications. Workout components of variance for lint index.
  • 2. Genotypes Replication I Replication II Replication III Total 1 36.5 36.0 36.4 108.9 2 51.0 54.0 55.5 160.5 3 42.1 44.8 42.9 129.8 4 46.5 51.0 49.5 147.0 5 49.5 48.0 47.3 144.8 6 51.0 49.5 51.5 152.0 7 50.0 49.0 49.8 148.8 8 54.5 52.0 55.5 162.0 9 50.5 48.5 52.0 151.0 10 53.8 54.0 53.5 161.3 11 57.0 56.0 55.0 168.0 12 52.5 51.0 50.0 153.5
  • 3. 13 47.5 48.0 48.5 144.0 14 57.0 56.0 57.5 170.5 15 39.0 37.0 40.0 116.0 16 47.5 49.0 48.0 144.5 17 60.0 61.0 62.0 183.0 18 61.0 62.5 61.5 185.0 19 46.5 48.5 45.0 140.0 20 47.0 47.5 48.5 143.0 21 54.0 55.0 55.5 164.5 22 48.0 48.5 49.5 146.0 23 47.0 45.5 47.0 139.5 24 58.5 58.0 57.5 174.0 25 55.5 57.5 56.5 169.5 Total 1263.4 1267.8 1275.9 3807.1
  • 4. Estimation of variability parameters (Components of variance) Estimation of PCV and GCV for replicated data Correction factor = = = 193253.46 Raw sum of squares = 36.52 + …………. 56.52 = 196058.55 (Grand Total)2 N (3807.1)2 75 ( ∑ x 2 )
  • 5. Total sum of squares = Raw S.S – C.F = 196058.55 – 193253.46 = 2805.09 Treatment sum of squares = 108.92 + ……. + 169.52 – C.F = 2708.59 Sum of squares of Treatment Total No. of rep = – C.F 587886.17 3 = – 193253.46
  • 6. Replication sum of squares = Error SS = Total SS – Treat SS – Rep SS = 93.28 Sum of squares of Rep.Total No. of Treatments = 1263.42 + …… 1275.92 25 = – 193253.46 3.22 – C.F
  • 7. Anova Sources of Variation df SS MSS SS df F.ratio Replication (r-1) 3-1 = 2 3.22 1.61 1.61 1.94 Treatment (t-1) 25-1 = 24 2708.59 112.86 112.86 1.94 Error (t-1) (r-1) 24 x 2 = 48 93.28 1.94 Total 74 2805.09 = 0.83 = 58.18
  • 8. F Table (48, 24) 5% 1.74 1% 2.20 If the calculated value is higher than the table value, it is significant. Treatments are differing significantly.
  • 9. Environmental variance (Ve) = EMS (Error Mean square) = 1.94 Genotypic variance (Vg) = = = 36.97 (Ms due to treatment – EMS) r 112.86 – 1.94 3
  • 10. Phenotypic variance (Vph) = Vg + Ve = 36.97 + 1.94 = 38.91 GCV = = 50.76 = = = 11.98% √Vg x x 100 3807.1 x = 75 √36.97 x 100 50.76 6.08 x 100 50.76
  • 12. ECV = = = 2.76% < 10 - low 10 - 20 - Moderate above 20 - High The PCV and GCV of lint index are moderate. It means that the variability is moderate. The influence of environment is low. √Ve x √1.94 x 100 50.76 x 100
  • 13. Heritability = = = 95.01% GA = K x √Vph x = 2.06 x √38.91 x = 2.06 x 6.24 x 0.95 = 12.21 Vg Vph x 100 36.97 38.91 x 100 Vg Vph 36.97 38.91
  • 14. GA as percent of Mean = = = 24.05 Inference 1. If the GCV is higher than PCV, it indicates that there is little influence of environment on the expression of character. Selection for such character will be rewarding. GA x x 100 12.21 50.76 x 100
  • 15. 2. If PCV is higher than GCV, it means that the apparent variation is not only due to genotypes but also due to influence of environment. Selection for such traits may be misleading. 3. If ECV is higher than PCV & GCV, it means the environment is playing a significant role in the expression of such character. Selection for the improvement of such character will be ineffective.
  • 16. R1 R2 R3 Total T1 65 95 84 244 T2 46 54 58 158 T3 62 68 67 197 T4 67 61 63 191 T5 75 77 71 223 T6 47 51 53 151 T7 61 66 69 196 Total 423 472 465 1360 Problem : 2 Calculate the components of variance.