Collection Of blood, sites Of blood collection in different animalsprecautions while blood collection

1. Collection Of blood, sites Of blood
collection in different animals
precautions while blood collection
By:Kashif Ali 2K20-Av-99

2. Blood Sample Collection Techniques:
◦ Remove the hair by shaving or clipping
◦ Swab the skin with alcohol, or tincture of Iodine to remove all excessive
contamination.
◦ Raise the vain by pressure, rubbing the site with alcohol help in outlining the vein
more clearly.
◦ Insert sterilized needle into vein.
◦ Gently aspirate the blood with syringe avoid collapse of the veins
◦ Remove the needle from syringe and allow the blood to flow out near the bottom of
the collection tube having anticoagulant.
◦ Do not shake vigorously to avoid rupturing of red cells.
◦ Note: Needle and syringes should preferably be of sterile disposable variety.
◦ The use of dirty needles should be avoided and on needle or syringe should not be
used to sample several animals.
◦ All syringes must e perfectly dry.
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3. Sites of Blood Collection
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4. Sites of Blood Collection
Horse:
◦ Blood is obtained from jugular vein in horse.
◦ Fine bore 3.5 c.m needle (21 G) and syringe or vacutainer is used to collect blood
◦ The vein is raised by pressure with left hand in lower half of the jugular furrow.
◦ Needle is inserted in the centre of vein and in an upward direction.
◦ Plunger of syringe is gently pulled out to facilitate collection.
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5. Sites of Blood Collection
Cattle, Sheep and Goat:
◦ Blood is obtained from Jugular vein,
◦ 5c.m 16 G needles is used.
◦ The vein is raised by digital pressure or with a rope.
◦ In lactating cattle the mammary vein is an alternative site.
◦ Blood can
◦ be collected from coccygeal vein in the base of the tail with
1 inch needle.
◦ The needle is inserted in the midline of tail at second to
third coccygeal intervertebral space to depth of an inch.
◦ Alternatively the needle is inserted until the bone reached
and then withdrawn slightly.
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6. Sites of Blood Collection
Dog and Cat:
◦ Blood is obtained from cephalic vein using 21 G needle and syringe.
◦ Vein is raised by gentle digital pressure.
◦ Needle is inserted into vein and blood is collected by gentle traction on the Syringe
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7. Containers for Sample Collection:
◦ Blood for hematology should be placed in bottles containing anticoagulants like
EDTA (Ethylene diamine tetra acetate) or Heparin. Needle should be removed from
syringe before collected blood sample is placed into glass tube.
◦ A variety of commercially available tubes can be used for blood collection. These
tubes are commonly known as vacutainer tubes.
◦ Vacutainers are commonly referred by their stopper color, which is used to identify
the type of anticoagulation system the tubes contain.
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8. Containers for Sample Collection:
1. The red-top tubes contain no anti-coagulant, also called plain tubes. These are used for serum
collection.
2. Lavender-Top Tube contains anticoagulant ethylene diamine tetra acetate (EDTA). This tube is
used to collect blood for hematological determination. The liquid tripotassium salt is the most
commonly used form of EDTA, and this is the most preferred for use preservation of cell volume
as measured on automated hematology analyzer. This also preserves morphological features on
stained film.
3. Green-Top or Heparin Tube contain heparin. This anticoagulant is used for certain special
biochemistry tests. particularly those that require a whole blood aliquot for determination and
that might be influenced by the presence of other chemical anticoagulant.
4. Blue -Top or Citrate Tube contain sodium citrate It is used for coagulation biochemistry
determination.
5. Sure Step Tube. This tube is variation of red top tube containing no anti-coagulant. The stopper is
red with black mottling, and the tube contain a gel that separate packed cell friction from serum,
thus prevent anyalyte metabolism from occurring at cell/fluid interface.
6. Gray-Tope or Fluoride Tubes contain sodium chloride. Flouride is not an anticoagulant, however.
Rather, it inhibits enzymes in glycolytic pathway and prevents erythrocyte, from metabolizing
glucose while whole blood is transported to laboratory.
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9. BASIC PRINCIPLES OF BLOOD COLLECTIONS
◦ Approaching The animal.
◦ Handling Needle and restraining of animal.
◦ Gage of material knowledge.
◦ The collecting Tube – type knowledge. (identification)
◦ Site and location of vein in the animal
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10. Procedure
Need following material:
◦ Choose Animal
◦ Hand gloves
◦ Tourniquet (or may use thumb pressure)
◦ Needle / syringe (of required gage)
◦ Vacutainer Tubes & its holder
◦ Alcohol pad & Bandage (confirm?)
Process:
◦ Approach the animal gently and try a friendly behavior, when it is comfortable try
to handle it as per its behavior.
◦ Once the Successfully animal is under control just start the collecting sampling
procedure. The sampler should have followed all the requirements while collection.
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11. Procedure
Process:
◦ Wear gloves, palpate the jugular vein or vein of the tail in case of cattle,
◦ apply pressure the over area of blood to vein, that done by banding may be a
tourniquet on the neck. Or simply applying thumb pressure.
◦ Inject the appropriate gage Syringe in 6 manner that it must be in inclined Position,
the sharpen edge or tip of needle must be inserted in and approach the vein.
◦ Make Sure, the needle is not out or inserted more deeply in the arteries, which can
lead to immediate Swelling of the certain area.
◦ Pull the plunger of the syringe and Collect the blood as Per animal’s Drawing range,
be move dangerous for the than that Could animal.
◦ blood is collected successfully, immediately put out the needle and inject into the
vacutainer which should be of Specificity, according to the analysis required.
◦ Apply the bandage or hold the blood by gauze paper.
◦ Now the Sampling is done The vaccutainer be moved for analysis.
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12. THANK YOU
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