Collection of blood, blood sites and anticoagulants used for blood collection for first Prof. DVM, First semester Faculty of A. H. Vety Sciences, SAU Tandojam

1. By
Dr. Allah Bux Kachiwal
Professor
Department of Veterinary Physiology and Biochemistry
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COLLECTION OF BLOOD, SITES OF BLOOD COLLECTION IN DIFFERENT
ANIMAL SPECIES, PRECAUTIONS WHILE BLOOD COLLECTION.

2. Blood Sample Collection Techniques
 Remove the hair by shaving or clipping
 Swab the skin with alcohol, ether or tincture of Iodine to remove all
excessive contamination.
 Raise the vain by pressure, rubbing the site with alcohol help in outlining
the vein more clearly.
 Insert sterilized needle into vein.
 Gently aspirate the blood with syringe to avoid collapse of the vein.
 Remove the needle from syringe and allow the blood to flow out near the
bottom of the collection tube having anticoagulant.
 Do not shake vigorously to avoid rupturing of red cells.
• Note: Needle and syringes should preferably be of sterile disposable
variety.
 The use of dirty needles should be avoided and on needle or syringe should
not be used to sample several animals.
 All syringes must e perfectly dry.

3. Sites of Blood Collection
Horse:
 Blood is obtained from jugular vein in horse.
 Fine bore 3.5 cm needle (21 G) and a syringe or
vacutainer is used to collect blood
 The vein is raised by pressure with left hand in
lower half of the jugular furrow.
 Needle is inserted in the centre of vein and in an
upward direction.
 Plunger of syringe is gently pulled out to facilitate
collection.

4. Collection of blood Jugular Vein

5. Cattle, Sheep and Goat:
• Blood is obtained from Jugular vein.
• 5cm, 16 G needles is used.
• The vein is raised by digital pressure or with a rope.
• In lactating cattle the mammary vein is an alternative site.
• Blood can be collected from coccygeal vein in the base of
the tail with 1 inch needle.
• The needle is inserted in the midline of tail at second to
third coccygeal intervertebral space to depth of an inch.
• Alternatively the needle is inserted until the bone reached
and then withdrawn slightly.

6. COLLECTION OF BLOOD JUGULAR
VEIN OF CATTLE

7. COLLECTION OF BLOOD FROM GOAT.

8. DOG AND CAT
 Blood is obtained from cephalic vein using 21G needle and
syringe.
 Vein is raised by gentle digital pressure.
 Needle is inserted into vein and blood is collected by gentle
traction on the Syringe.

9. BLOOD COLLECTION FROM CAT

10. CONTAINERS FOR SAMPLE
COLLECTION:
 Blood for hematology should be placed in bottles
containing anticoagulants like EDTA (Ethylene
diamine tetra acetate) or Heparin. Needle should
be removed from syringe before collected blood
sample is placed into glass tube.
 A variety of commercially available tubes can be
used for blood collection. These tubes are
commonly known as vacutainer tubes.
• Vacutainers are commonly referred by their
stopper color, which is used to identify the type
of anticoagulation system the tubes contain.

11. TYPES OF VACUTAINERS

12. TYPES OF VACUTAINERS
1) The red-top tubes contain no anti-coagulant, also called
plain tubes. These are used for serum collection.
2) Lavender-Top Tube contains anticoagulant ethylene
diamine tetra acetate (EDTA). This tube is used to
collect blood for hematological determination. The
liquid tripotassium salt is the most commonly used
form of EDTA, and this is the most preferred for use in
preservation of cell volume as measured on automated
hematology analyzer. This also preserves
morphological features on stained film.

13. TYPES OF VACUTAINERS
1) Green-Top or Heparin Tube: The green –top tube contain heparin. This
anticoagulant is used for certain special biochemistry tests, particularly
those that require a whole blood aliquot for determination and that might be
influenced by the presence of other chemical anticoagulant.
2) Blue –Top or Citrate Tube: The blue –top tube contain sodium citrate. It is
used for coagulation biochemistry determination.
3) Sure Step Tube- This tube is variation of the rd –top tube containing no
anti-coagulant. The stopper is red with black mottling, and the tube contain
a gel that separate packed cell friction from serum, thus prevent anyalyte
metabolism from occurring at cell/fluid interface.
4) Gray-Tope or Flouride Tubes- The grey- top tubes contain sodium chloride.
Flouride is not an anticoagulant, however. Rather, it inhibits enzymes in
glycolytic pathway and prevents erythrocyte, from metabolizing glucose
while whole blood is transported to laboratory.

14. https://www.facebook.com/ABKachiwal/
https://www.youtube.com/user/kachiwal2003
Email: kachiwal2003@gmail.com
abkachiwal@sau.edu.com
Watsup# 03003058466