A micro - manipulator is a device which is used to physically interact with a sample under a microscope. It is usually used in conjuction with microscope. It is the method used in the isolation of pure culture technique.

1. BY,
HANU PRATAP

2.  A micromanipulator is a device which is used to
physically interact with a sample under a
microscope
 It may typically consist of an input joystick ,
a mechanism for reducing the range of
movement and an output section with the
means of holding a microtool to hold, inject,
cut or otherwise manipulate the object as
required
 Micromanipulators are usually used in
conjuction with microscope.
 It is the method used in the isolation of pure
culture technique

4. Micromanipulator have been
built, which permit one to pick
out a single cell from a mixed
culture.
This instrument is used in
conjuction with a microscope to
pick a single cell (particularly
bacterial cell)from a hanging
drop preparation.

5. The micromanipulator have a micrometer
adjustments by means of which its
micropipette can be move right and left,
forward and backward, and up and down.
A series of hanging drop of a diluted
culture are placed on a special sterile
coverslip by a micropipette.
Now a hanging drop is searched, which
contains only a single microorganism cell

7. This cell is drawn into the micropipette
by gentle suction and then transferred to
a large drop of sterile medium on another
sterile cover slip.
When the number of cell increases in that
drop as a result of multiplication, the
drop is transferred to a culture tube
having suitable medium.
This yield a pure culture of the required
microorganism.

9. The advantage of this method are
that can be reasonably sure that
the cultures come from a single
cell and one can obtain strains
with in the species.

10. Equipment is expensive.
Its manipulation is very tedious.
It required a skilled operator.