Isolation of Pure culture

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for isolation of different micro organisms it is necessary to obtain their pure culture, for this there are 3 methods spread plate, pour plate, streak plate methods.

Science

ISOLATION OF PURE CULTURES
BY,
HANU PRATAP

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PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-V Part-1 Types of spoilage, factors affecting the microbial spoilage of pharmaceutical products, source and type of contaminants. Introduction: Defintion Types of Microbial Spoilage: 1. Infection induced due to contaminated pharmaceutical products: Table no. 1.1 Common pathogens spoiling pharmaceutical products: 2. Physicochemical spoilage – i) Viable growth ii) Gas production iii) Colouration / Decolouration iv) Odour formation v) Taste change 3. Physical Spoilage: Cracking of emulsion: Odor changes 4. Biological spoilage: Microbial Toxins Microbial Metabolites 5. Chemical spoilage: Table 1.2 Susceptibility of pharmaceutical ingredients to microbial contamination Factors affecting microbial spoilage Size of contaminant inoculum Nutritional factors Moisture content pH Storage temperature Redox potential Packaging design Sources and Types Of Contamination: Personnel, Poor facility design, Incoming ventilation air, Machinery and other equipment for production, Raw material and semi-finished material, Packaging material, Utilities, Different media used in the production process as well as for cleaning and Cleanroom clothing.

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It has been developed for the detection, enumeration & identification of bacteria & yeasts in clinical specimens. It is an instrument used for automatic computer-assisted identification of bacteria It mainly involves staining, motility test, cultural characteristics, a series of biochemical tests. The automatic bacteria identification system automatically identifies the bacteria in very short time.

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Complete Glycolysis in short or easy way to understand Glycolysis is derived from the Greek words glykys = sweet and lysis = splitting. This pathway was described by EMBDEN,MEYERHOFF and PARNAS. Hence, it is also called EMP PATHWAY. glycolysis is the process in which 1 molecule of glucose broken down to form 2 molecules of pyruvic acid.thus, 4 ATP molecules are synthesised and 2 ATP molecules are used during glycolysis. it occur in cytoplasm of animal cells,plant cell.

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