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Green Synthesis of Silver
Nanoparticles Using Helichrysum
graveolens for Biomedical
Applications and Wastewater
Treatment
Dani George
199307
MSc. Analytical Chemistry
1
2
Overview
Journal
Paper
Introduction
Plant
Synthesis and characterization
Conclusion
reference
3
Journal
Title Journal of Bionanoscience
Publication Type journal
Subject Area, Categories, Scope Biotechnology (Q3); Biomaterials (Q4); Biomedical Engineering
(Q4); Nanoscience and Nanotechnology (Q4)
h-index 17
Overall Rank/Ranking 17827
SCImago Journal Rank (SJR) 0.202
Impact Factor 1.05
Publisher American Scientific Publishers
Country United States
ISSN 15577929, 15577910
4
Paper
DOI:https://doi.org/10.10s
Mohammad Ehsan Taghavizadeh
Yazdi,
Mohammad Sadegh Amiri,
Setareh Akbari,
Mohammad Sharifalhoseini,
Fahimeh Nourbakhsh,
Mohammad Mashreghi,
EhsanYousefi,
Mohammad Reza Abbasi,
Masoomeh Modarres &
Ali Es-haghi
Authors:
Publishers: BioNanoScience
Introduction
• AgNps synthesized from Helichrysum
Graveolens.
• Structure described by TEM, UV-VIS, FTIR
• Antimicrobial activity
• Anticancer activity against colon cancer
cell
• Green catalyst behavior
•
5
Helichrysum graveolens (SWEET)
Family:Compositae
Tribe: Gnaphalieae
Genus: Helichrysum; Mill.,
•Shoot part is collected, rinsed and dried
•Filtrate used as reducing agent for
AgNps synthesis
•Applied in a variety of medical
treatments including choleretic, diuretic,
anti-inflammatory,
and detoxification
6
Synthesis and characterization
AgNO3(0.1MOL/L) + Plant extract
6hr stirring
The mixture of shoots’ extract of H. graveolens
and AgNO3 (right)
and biofabricated AgNPs (left)
7
1.UV-VISIBLE
Fabrication of nanoparticles were Characterized.
AgNPs displayed peak absorbance at 439 nm
8
2.FTIR Results
Applied to identify the potential molecules accountable for
the fabrication of the AgNPs
peak at 3382 cm−1
3382 (O–H stretching, H-
bonded), 2927 (C–Ostretching,
free),
1608 (C=O stretching),
1513 (C–N stretching
vibration), 1417 (O–H bend),
1035 (C–N stretching),
820 (C–O stretching) 606 (C–X
stretchingvibration) cm−1.
9
3.TEM Results
The form and plan of AgNPs were recognized using TEM. The
images showed that biosynthesized AgNPs are spherical
The mean diameter size of nanoparticles was almost
11 nm. Also, the histogram of size dispersal is designated.
10
1.Antimicrobial test
Escherichia coli and P.aeruginosa
:gram-negative bacteria
S.epidermidis and S. aureus
:gram positive bacteria
11
12
2.Photocyatalytic activity
Effect of extract and nanoparticle catalysts
on the degradation of MO.
13
3.Cell toxicity of AgNps
AgNPs with the plant extract could destroy the cancer cells depending
on the concentration and time
Highest lethal effect on cancer cells at concentrations of 5 μg/mL and
for
72 h.
14
CONCLUSION
easy, cheap, and eco-friendly method.
AgNPs showed effectual
antibacterial efficacy against gram-negative and
positive bacteria.
High anticancer activity :numerous biology-
biomedicine
applications.
Potential materials to treat the wastewater from
industries.
15
Reference
Synthesis of silver nanoparticles and their optical Properties
: https://doi.org/10.1080/17458080903583915
Role of Ribes khorassanicum in the biosynthesis of AgNPs and their
antibacterial properties
IET Nanobiotechnology, 13(2), 189–192
Green synthesis and characterization of zinc oxide nanoparticles with
antibacterial and antifungal activity. Journal of Molecular Structure,
128107.
https://doi.org/10.1016/j.molstruc.2020.128107.
16
THANK YOU

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Green synthesis of silver nps and appli

  • 1. Green Synthesis of Silver Nanoparticles Using Helichrysum graveolens for Biomedical Applications and Wastewater Treatment Dani George 199307 MSc. Analytical Chemistry 1
  • 3. 3 Journal Title Journal of Bionanoscience Publication Type journal Subject Area, Categories, Scope Biotechnology (Q3); Biomaterials (Q4); Biomedical Engineering (Q4); Nanoscience and Nanotechnology (Q4) h-index 17 Overall Rank/Ranking 17827 SCImago Journal Rank (SJR) 0.202 Impact Factor 1.05 Publisher American Scientific Publishers Country United States ISSN 15577929, 15577910
  • 4. 4 Paper DOI:https://doi.org/10.10s Mohammad Ehsan Taghavizadeh Yazdi, Mohammad Sadegh Amiri, Setareh Akbari, Mohammad Sharifalhoseini, Fahimeh Nourbakhsh, Mohammad Mashreghi, EhsanYousefi, Mohammad Reza Abbasi, Masoomeh Modarres & Ali Es-haghi Authors: Publishers: BioNanoScience
  • 5. Introduction • AgNps synthesized from Helichrysum Graveolens. • Structure described by TEM, UV-VIS, FTIR • Antimicrobial activity • Anticancer activity against colon cancer cell • Green catalyst behavior • 5
  • 6. Helichrysum graveolens (SWEET) Family:Compositae Tribe: Gnaphalieae Genus: Helichrysum; Mill., •Shoot part is collected, rinsed and dried •Filtrate used as reducing agent for AgNps synthesis •Applied in a variety of medical treatments including choleretic, diuretic, anti-inflammatory, and detoxification 6
  • 7. Synthesis and characterization AgNO3(0.1MOL/L) + Plant extract 6hr stirring The mixture of shoots’ extract of H. graveolens and AgNO3 (right) and biofabricated AgNPs (left) 7
  • 8. 1.UV-VISIBLE Fabrication of nanoparticles were Characterized. AgNPs displayed peak absorbance at 439 nm 8
  • 9. 2.FTIR Results Applied to identify the potential molecules accountable for the fabrication of the AgNPs peak at 3382 cm−1 3382 (O–H stretching, H- bonded), 2927 (C–Ostretching, free), 1608 (C=O stretching), 1513 (C–N stretching vibration), 1417 (O–H bend), 1035 (C–N stretching), 820 (C–O stretching) 606 (C–X stretchingvibration) cm−1. 9
  • 10. 3.TEM Results The form and plan of AgNPs were recognized using TEM. The images showed that biosynthesized AgNPs are spherical The mean diameter size of nanoparticles was almost 11 nm. Also, the histogram of size dispersal is designated. 10
  • 11. 1.Antimicrobial test Escherichia coli and P.aeruginosa :gram-negative bacteria S.epidermidis and S. aureus :gram positive bacteria 11
  • 12. 12 2.Photocyatalytic activity Effect of extract and nanoparticle catalysts on the degradation of MO.
  • 13. 13 3.Cell toxicity of AgNps AgNPs with the plant extract could destroy the cancer cells depending on the concentration and time Highest lethal effect on cancer cells at concentrations of 5 μg/mL and for 72 h.
  • 14. 14 CONCLUSION easy, cheap, and eco-friendly method. AgNPs showed effectual antibacterial efficacy against gram-negative and positive bacteria. High anticancer activity :numerous biology- biomedicine applications. Potential materials to treat the wastewater from industries.
  • 15. 15 Reference Synthesis of silver nanoparticles and their optical Properties : https://doi.org/10.1080/17458080903583915 Role of Ribes khorassanicum in the biosynthesis of AgNPs and their antibacterial properties IET Nanobiotechnology, 13(2), 189–192 Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity. Journal of Molecular Structure, 128107. https://doi.org/10.1016/j.molstruc.2020.128107.